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Identification of a novel cytochrome P450 CYP321B1 gene from tobacco cutworm （Spodoptera litura） and RNA interference to evaluate its role in commonly used insecticides

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Insect Science 2017年第2期24卷 235-247页

作者： Rui-Long Wang Keyan Zhu-Salzman Scott R. Baerson Xiao-Wei Xin Jun Li yi-juan su Ren-Sen Zeng Key Laboratory of Tropical Agro-Environment Ministry of Agriculture College of Natural Resources and Environment South China Agri- cultural University Guangzhou China College of Life Sciences Fujian Agriculture and Forestry University Fuzhou China Departments of Entomology Texas A&M University Texas United States Department of Agriculture-Agricultural Research Service Natural Products Utilization Research Unit University Mississippi USA Guangdong Engineering Research Center for Modem Eco-agriculture and Circular Agriculture Guangzhou China

Insect cytochrome P450 monooxygenases （CYPs or P450s） play an important role in detoxifying insecticides leading to resistance in insect populations. A polyphagous pest, Spodoptera litura, has developed resistance to a wide range of insecticides. In the present study, a novel P450 gene, CYP321B1, was cloned from S. litura. The function of CYP321B1 was assessed using RNA interference （RNAi） and monitoring resistance levels for three commonly used insecticides, including chlorpyrifos, β-cypermethrin and methomyl. The full-length complementary DNA sequence of CYP321B1 is 1814 bp long with an open reading frame of 1 488 bp encoding 495 amino acid residues. Quantitative reverse-transcriptase polymerase chain reaction analyses during larval and pupal develop- ment indicated that CYP321B1 expression was highest in the midgut of fifth-instar larvae, followed by fat body and cuticle. The expression of CYP321B1 in the midgut was up- regulated by chlorpyrifos,β-cypermethrin and methomyl with both lethal concentration at 15% （LC15）（50, 100 and 150 μg/mL, respectively） and 50%（LC50） dosages （100,200 and 300μg/mL, respectively）. Addition of piperonyl butoxide （PBO） significantly increased the toxicity ofchlorpyrifos,β-cypermethrin and methomyl to S. litura, suggesting a marked synergism of the three insecticides with PBO and P450-mediated detoxification. RNAi- mediated silencing of CYP321B1 further increased mortality by 25.6% and 38.9% when the fifth-instar larvae were exposed to chlorpyrifos and β-cypermethrin, respectively, at the LCso dose levels. The results demonstrate that CYP321B1 might play an important role in chlorpyrifos and β-cypermethrin detoxification in S. litura.

关键词： CYP321B1 cytochrome P450 monooxygenases insecticide resistance RNAinterference Spodoptera litura

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A surface engineering strategy for the stabilization of zinc metal anodes with montmorillonite layers toward long-life rechargeable aqueous zinc ion batteries

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Journal of Energy Chemistry 2025年第1期100卷 94-105页

作者： Wenbo Wang Ruifeng Xu Xu Zhang Peiyu Wang Bao Yang Bingjun Yang juan Yang Kailimai su Pengjun Ma Yanan Deng Xianfeng Fan Wanjun Chen Key Laboratory for Electronic Materials College of Electrical Engineering Northwest Minzu University Resource Chemistry and Energy Materials Research Center State Key Laboratory of Solid Lubrication Lanzhou Institute of Chemical Physics Chinese Academy of Sciences University of Chinese Academy of Sciences Institute for Materials and Processes School of Engineering University of Edinburgh

Rechargeable aqueous zinc-ion batteries （AZIBs） exhibit appreciable potential in the domain of electrochemical energy ***,there are serious challenges for AZIBs,for instance zinc dendrite growth,hydrogen evolution reaction （HER）,and corrosion side ***,we propose a surface engineering modification strategy for coating the montmorillonite （MMT） layer onto the surface of the Zn anode to tackle these issues,thereby achieving high cycling stability for rechargeable *** results reveal that the MMT layer on the surface of the Zn anode is able to provide ordered zincophilic channels for zinc ions migration,facilitating the reaction kinetics of zinc *** functional theory（DFT） calculations and water contact angle （CA） tests prove that MMT@Zn anode exhibits superior adsorption capacity for Zn2+and better hydrophobicity than the bare Zn anode,thereby achieving excellent cycling ***,the MMT@Zn||MMT@Zn symmetric cell holds the stable cycling over 5600 h at 0.5 mA cm-2and 0.125 m A h cm-2,even exceeding 1800 h long cycling under harsh conditions of 5 m A cm-2and 1.25 m A h *** MMT@Zn||V2O5full cell reaches over 3000 cycles at 2 A g-1with excellent rate ***,this surface engineering modification strategy for enhancing the electrochemical performance of AZIBs represents a promising application.

关键词： surface engineering strategy Montmorillonite layer Protection mechanism of Zn anode Cycling stability Aqueous zinc ion batteries

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多种开采方式对覆岩裂隙发育高度的影响研究

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内蒙古煤炭经济 2019年第8期 23-23,66页

作者：施莉易素娟江西省煤田地质勘察研究院

不同开采方式对煤层破坏的影响不同。本文采用数值模拟方法对垮落法开采、分层开采和充填开采的覆岩裂隙发育演化规律进行研究。利用FLAC3D数值模拟平台,对3种开采方式下工作面顶板裂隙带高度随工作面推进变化进行系统分析,得到垮落法... 详细信息

不同开采方式对煤层破坏的影响不同。本文采用数值模拟方法对垮落法开采、分层开采和充填开采的覆岩裂隙发育演化规律进行研究。利用FLAC3D数值模拟平台,对3种开采方式下工作面顶板裂隙带高度随工作面推进变化进行系统分析,得到垮落法开采覆岩裂隙发育高度最大,分层开采次之,充填开采最小。并研究了某矿数值模拟和现场试验结果,验证了数值结果的可靠性。

关键词：开采方法垮落法开采分层开采充填开采覆岩裂隙

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Lipomatous ependymoma with ZFTA:RELA fusion-positive:A case report

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World Journal of Clinical Cases 2025年第1期13卷 48-55页

作者： Xiao-Yu Zhao juan-Han Yu yi-Hua Wang yi-Xin Liu Lu Xu Lin Fu Ning yi China Medical University The First Hospital of China Medical UniversityShenyang 110001Liaoning ProvinceChina Department of Pathology The First Hospital of China Medical UniversityShenyang 110001Liaoning ProvinceChina Anshan Cancer Hospital Anshan 114000Liaoning ProvinceChina

BACKGROUND Ependymoma with lipomatous differentiation is a rare type of *** ZFTA fusion-positive supratentorial ependymoma is a novel tumor type in the 2021 World Health Organization classification of central nervous system *** fusion-positive lipomatous ependymoma has not been reported to *** suMMARY We reported a case of a 15-year-old Chinese male who had a sudden convulsion lasting approximately six *** resonance imaging showed a round cystic shadow of approximately 1.9 cm×1.5 cm×1.9 cm under the right parieto-occipital *** examination showed characteristic perivascular pseudorosettes and adipose differentiation in the ***-chemical staining showed that the tumor cells were negative for cytokeratin,NeuN,Syn and p53,but positive for GFAP,vimentin and S-100 ***-ficant punctate intracytoplasmic EMA immunoreactivity was *** level of Ki-67 was about 5%.Genetic analysis revealed ZFTA:RELA fusion.A cranio-tomy with total excision of the tumor was *** follow-up time was 36 months,no evidence of disease recurrence was found in magnetic resonance *** Based on these findings,the patient was diagnosed as a ependymoma with ZFTA fusion and lipomatous *** case report provides information on the microscopic morphological features of ependymoma with ZFTA fusion and lipomatous differentiation,which can help pathologists to make a definitive diagnosis of this tumor.

关键词： Ependymoma Lipomatous Molecular classification supratentorial ZFTA:RELA fusion Case report

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山西省土地沙质荒漠化现状及发展趋势研究

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水土保持学报 2003年第6期17卷 81-84页

作者：马义娟苏志珠太原师范学院地理系山西太原030012 山西大学黄土高原研究所山西太原030006

山西省的晋西北和大同地区是全国荒漠化监测与防治的重点地区之一,也是环京津唐地区防风固沙、绿色生态屏障建设的重点地区之一。该区由风力侵蚀形成的沙质荒漠化土地分布集中,且危害较为严重。沙质荒漠化土地的发生与扩展是自然因素和... 详细信息

山西省的晋西北和大同地区是全国荒漠化监测与防治的重点地区之一,也是环京津唐地区防风固沙、绿色生态屏障建设的重点地区之一。该区由风力侵蚀形成的沙质荒漠化土地分布集中,且危害较为严重。沙质荒漠化土地的发生与扩展是自然因素和人为因素综合作用的结果,气候干旱多风、地表沙物质丰富和植被稀疏等自然因素的存在,本身就孕育着沙质荒漠化发生与发展的自然过程,即沙质荒漠化的自然过程,而人类不合理的经济活动则是叠加在沙质荒漠化自然过程的基础上起加速、加剧作用,即沙质荒漠化的人为加速过程。动态监测资料表明流动沙地而积有增加趋势,在未来中国北方气候变暖干化背景下,随着人类活动的加强该区沙质荒漠化有加速发展的趋势,应引起高度重视。

关键词：土地沙质荒漠化山西省

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Roles of the pretectum in prey capture of zebrafish larvae

Roles of the pretectum in prey capture of zebrafish larvae

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中国神经科学学会第九届全国学术会议暨第五次会员代表大会

作者： Bai-Bing ZHANG,Xiao-Quan LI,Yu MU,Shu-yi ZHANG,Yu-juan su,Jiu-Lin DU~* Laboratory of Sensory Integration & Behavior,Institute of Neuroscience,Shanghai Institutes of Biological Sciences, Chinese Academy of Sciences,Shanghai 200031,China

＜正＞Objective The pretectum,a region between the thalamus and midbrain,receives binocular sensory inputs from retinal ganglion cells（RGCs）and sends output to the optic *** studies in amphibians suggest important roles of the pretectum in prey capture *** larvae develop the ability to capture prey as early as 5 days post-fertilization（dpf）.Zebrafish pretectum mainly consists of three types of neurons,including dopaminergic, serotoninergic and GABAergic cells,implying its modulatory roles in prey *** present study,we examined how the pretectum regulates prey capture at both neural circuit and behavior *** The morphology of pretectal dopaminergic neurons was revealed by using the transgenic line ETvmat2:GFP,in which monoaminergic neurons express GFP,and Kaede-based photo-conversion in vivo ***,defects in the Paramecium capture behavior of zebrafish larvae with laser ablation of pretectal dopaminergic neurons were ***,in vivo calcium imaging of both tectal and pretectal neurons was carried on to study their response evoked by prey-like cues. Finally,the detection of dopamine release by carbon fiber and pharmacological manipulation of dopamine signaling were used to examine the role of dopaminergic neurons in modulation of optic tectal *** We found that there was a dopaminergic neuron cluster in the pretectum that projected to the deep layer of the tectal neuropil. Focal ablation of these neurons impaired Paramecium capture *** data showed that some sparsely distributed tectal neurons responded to prey-like visual *** experiments will be performed to figure out how pretectal dopaminergic neurons modulate prey-like cue-induced responses in tectal *** The pretectum regulates prey capture behavior through dopaminergic neuromodulation of tectal function.

关键词： zebrafish pretecum optic tectum prey capture dopaminergic neuron in vivo imaging

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World Journal of Clinical Cases 2025年第6期13卷 24-29页

作者： Wen-Jin Yuan yi-juan Zheng Bing-Rong Zhang yi-Jie Lin You Li Yan-Yan Qiu Xue-Ping Yu Department of Infection Disease Clinical Medical Research Center for Bacterial and Fungal Infectious Diseases of Fujian provinceFujian Medical University Affiliated First Quanzhou HospitalQuanzhou 362000Fujian ProvinceChina

BACKGROUND Hydroxyurea,an antimetabolite,is frequently prescribed for various hemato-logical disorders,and its common side effects include gastrointestinal problems,cutaneous or mucosal lesions and pyrexia/*** suMMARY This study reports the case of a 67-year-old woman who developed recurrent abdominal pain after 10 years of continuous hydroxyurea therapy for primary *** revealed an ileocecal *** discontinuing hydroxyurea therapy for 6 months,follow-up colonoscopy showed a significant reduction in the *** We consider cecal ulcers as a rare complication of hydroxyurea therapy which typically resolves upon stopping the drug.

关键词： Hydroxyurea Ulcer Abdominal pain Thrombocythemia Colonoscopy Case report

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Near-infrared brain functional characteristics of mild cognitive impairment with sleep disorders

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World Journal of Psychiatry 2025年第1期15卷 106-116页

作者： Heng Liao Sha Liao Yu-Jiao Gao Xi Wang Li-Hong Guo su Zheng Wu Yang yi-Nan Dai Sleep Psychosomatic Medicine Center Taihe Hospital of Shiyan CityAffiliated Hospital of Hubei University of MedicineShiyan 442000Hubei ProvinceChina Department of Integrated Chinese and Western Medicine Taihe Hospital of Shiyan CityAffiliated Hospital of Hubei University of MedicineShiyan 442000Hubei ProvinceChina Department of Orthopaedics Taihe Hospital of Shiyan CityAffiliated Hospital of Hubei University of MedicineShiyan 442000Hubei ProvinceChina Department of Rehabilitation Taihe Hospital of Shiyan CityAffiliated Hospital of Hubei University of MedicineShiyan 442000Hubei ProvinceChina

BACKGROUND Mild cognitive impairment(MCI)has a high risk of progression to Alzheimer’s *** disease is often accompanied by sleep disorders,and whether sleep disorders have an effect on brain function in patients with MCI is *** To explore the near-infrared brain function characteristics of MCI with sleep *** A total of 120 patients with MCI(MCI group)and 50 healthy subjects(control group)were *** subjects underwent the functional near-infrared spec-troscopy *** baseline data,Mini-Mental State Examination,Montreal Cognitive Assessment scale,fatigue severity scale(FSS)score,sleep parameter,and oxyhemoglobin(Oxy-Hb)concentration and peak time of functional near-infrared spectroscopy test during the task *** relationship between Oxy-REsuLTS Compared with the control group,the FSS score of the MCI group was higher(t=11.310),and the scores of Pittsburgh sleep quality index,sleep time,sleep efficiency,nocturnal sleep disturbance,and daytime dysfunction were higher(Z=-10.518,-10.368,-9.035,-10.661,-10.088).subjective sleep quality and total sleep time scores were lower(Z=-11.592,-9.924).The sleep efficiency of the MCI group was lower,and the awakening frequency,rem sleep latency period,total sleep time,and oxygen desaturation index were higher(t=5.969,5.829,2.887,3.003,5.937).The Oxy-Hb concentration at T0,T1,and T2 in the MCI group was lower(t=14.940,11.280,5.721),and the peak time was higher(t=18.800,13.350,9.827).In MCI patients,the concentration of Oxy-Hb during T0 was negatively correlated with the scores of Pittsburgh sleep quality index,sleep time,total sleep time,and sleep efficiency(r=-0.611,-0.388,-0.563,-0.356).It was positively correlated with sleep efficiency and total sleep time(r=0.754,0.650),and negatively correlated with oxygen desaturation index(r=-0.561)and FSS score(r=-0.526).All comparisons were P<*** Patients with MCI and sleep disorders have lower near-infrared brain function than norm

关键词： Mild Cognitive impairment Sleep disorders Near-infrared Brain functional Characteristics

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晋西黄土高原环境退化及生态建设对策

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山地学报 2001年第3期19卷 278-281页

作者：马义娟苏志珠山西大学师范学院地理系!山西太原030012 山西大学黄土高原研究所!山西太原030006

晋西是黄土高原的重要组成部分 ,环境退化严重地制约了该区社会经济的可持续发展。根据野外调查和前人成果 ,讨论了该区环境退化的表现形式及危害 ,并对环境退化的防治和生态建设提出了若干对策

关键词：环境退化生态建设晋西黄土高原

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Maintaining moderate levels of hypochlorous acid promotes neural stem cell proliferation and differentiation in the recovery phase of stroke

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Neural Regeneration Research 2025年第3期20卷 845-857页

作者： Lin-Yan Huang yi-De Zhang Jie Chen Hai-Di Fan Wan Wang Bin Wang Ju-Yun Ma Peng-Peng Li Hai-Wei Pu Xin-yian Guo Jian-Gang Shen su-Hua Qi School of Medical Technology Xuzhou Key Laboratory of Laboratory DiagnosticsXuzhou Medical UniversityXuzhouJiangsu ProvinceChina School of Pharmacy Xuzhou Medical UniversityXuzhouJiangsu ProvinceChina Department of Laboratory Medicine Branch Hospital of Huai’an First People’s HospitalHuai’anJiangsu ProvinceChina Department of Laboratory Medicine the Affiliated Hospital of Xuzhou Medical UniversityXuzhouJiangsu ProvinceChina School of Chinese Medicine The University of Hong KongHong Kong Special Administrative RegionChina

It has been shown clinically that continuous removal of ischemia/reperfusion-induced reactive oxygen species is not conducive to the recovery of late ***,previous studies have shown that excessive increases in hypochlorous acid after stroke can cause severe damage to brain *** previous studies have found that a small amount of hypochlorous acid still exists in the later stage of stroke,but its specific role and mechanism are currently *** simulate stroke in vivo,a middle cerebral artery occlusion rat model was established,with an oxygen-glucose deprivation/reoxygenation model established in vitro to mimic *** found that in the early stage(within 24 hours)of ischemic stroke,neutrophils produced a large amount of hypochlorous acid,while in the recovery phase(10 days after stroke),microglia were activated and produced a small amount of hypochlorous ***,in acute stroke in rats,hypochlorous acid production was prevented using a hypochlorous acid scavenger,taurine,or myeloperoxidase inhibitor,4-aminobenzoic acid *** results showed that high levels of hypochlorous acid(200μM)induced neuronal apoptosis after oxygen/glucose deprivation/***,in the recovery phase of the middle cerebral artery occlusion model,a moderate level of hypochlorous acid promoted the proliferation and differentiation of neural stem cells into neurons and *** suggests that hypochlorous acid plays different roles at different phases of cerebral ischemia/reperfusion *** levels of hypochlorous acid(5 and 100μM)promoted nuclear translocation ofβ-*** transfection of single-site mutation plasmids,we found that hypochlorous acid induced chlorination of theβ-catenin tyrosine 30 residue,which promoted nuclear ***,our study indicates that maintaining low levels of hypochlorous acid plays a key role in the recovery of neurological function.

关键词： cell differentiation cerebral ischemia/reperfusion injury chlorination hypochlorous acid microglia neural stem cell neurogenesis nuclear translocation stroke β-catenin

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