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Terpenoid Indole Alkaloids Biosynthesis and Metabolic Engineering in Catharanthus roseus

Journal of Integrative Plant Biology 2007年第7期49卷 961-974页

作者： Dong-Hui Liu Hong-Bin Jin Yu-Hui Chen Li-Jie Cui Wei-Wei Ren yi-fu gong Ke-Xuan Tang Shanghai Key Laboratory of Agrobiotechnology Plant Biotechnology Research Center Fudan-Shanghai Jiaotong University ( SJTU)-Nottingham Plant Biotechnology R & D Center School of Agriculture and Biology Shanghai Jiaotong University Shanghai 200030 China Department of Biochemistry and Molecular Biology Shenyang Medical College Shenyang 110034 China Faculty of Life Sciences and Biotechnology Ningbo University Ningbo 315211 China State Key Laboratory of Genetic Engineering School of Life Sciences Fudan-SJTU-Nottingham Plant Biotechnology R & D Center Morgan-Tan International Center for Life Sciences Fudan University Shanghai 200433 China

Catharanthus roseus L. （Madagascar periwinkle） biosynthesizes a diverse array of secondary metabolites including anticancer dimeric alkaloids （vinblastine and vincristine） and antihypertensive alkaloids （ajmalicine and serpentine）. The multi-step terpenoid indole alkaloids （TIAs） biosynthetic pathway in C. roseus is complex and is under strict molecular regulation. Many enzymes and genes involved in the TIAs biosynthesis have been studied in recent decades. Moreover, some regulatory proteins were found recently to control the production of TIAs in C. roseus. Based on mastering the rough scheme of the pathway and cloning the related genes, metabolic engineering of TIAs biosynthesis has been studied in C. roseus aiming at increasing the desired secondary metabolites in the past few years. The present article summarizes recent advances in isolation and characterization of TIAs biosynthesis genes and transcriptional regulators involved in the second metabolic control in C. roseus. Metabolic engineering applications in TIAs pathway via overexpression of these genes and regulators in C. roseus are also discussed.

关键词： biosynthesis pathway Catharanthus roseus metabolic engineering terpenoid indole alkaloids.

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A New Farnesyl Diphosphate Synthase Gene from Taxus media Rehder:Cloning,Characterization and functional Complementation

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Journal of Integrative Plant Biology 2006年第6期48卷 692-699页

作者： Zhi-Hua Liao Min Chen yi-fu gong Zhu-Gang Li Kai-Jing Zuo Peng Wang Feng Tan Xiao-Fen Sun Ke-Xuan Tang State Key Laboratory of Genetic Engineering School of Life Sciences Fudan-SJTU-Nottingham Plant Biotechnology R＆D Center Morgan-Tan International Center for Life Sciences Fudan University Shanghai 200433 China Laboratory of Natural Products and Metabofic Engineering Institute of Biotechnology Chongqing Sweetpotato Research Center School of Life Sciences Southwest University Chongqing 400715 China Plant Biotechnology Research Center Fudan-SJTU-Nottingham Plant Biotechnology R＆D Center School of Agriculture and Biology Shanghai Jiaotong University Shanghai 200030 China Faculty of Life Sciences and Biotechnology Ningbo University Ningbo 315211 China

Farnesyl dlphosphate synthase （FPS; EC 2.***.1.10） catalyzes the production of 15-carbon farnesyl dlphosphate which Is a branch-point Intermediate for many terpenoids. This reaction Is considered to be a ratelimiting step In terpenold biosynthesis. Here we report for the first time the cloning of a new full-length cDNA encoding farnesyl dlphosphate synthase from a gymnosperm plant species, Taxus media Rehder, designated as TmFPS1. The full-length cDNA of TmFPS1 （GenBank accession number： AY461811） was 1 464 bp with a 1 056-bp open reading frame encoding a 351-amino acid polypeptlde with a calculated molecular weight of 40.3 kDa and a theoretical pl of 5.07. Biolnformatlc analysis revealed that TmFPS1 contained all five conserved domains of prenyltransferases, and showed homology to other FPSs of plant origin. Phylogenetlc analysis showed that farnesyl dlphosphate synthases can be divided Into two groups： one of prokaryotic origin and the other of eukaryotic origin. TmFPS1 was grouped with FPSs of plant origin. Homologybased structural modeling showed that TmFPS1 had the typical spatial structure of FPS, whose most prominent structural feature Is the arrangement of 13 core helices around a large central cavity In which the catalytic reaction takes place. Our blolnformatic analysis strongly suggests that TmFPS1 is a functional gene. Southern blot analysis revealed that TmFPS1 belongs to a small FPSgene family in T. media. Northern blot analysis indicated that TmFPS1 is expressed in all tested tissues, Including the needles, stems and roots of T. media. Subsequently, functional complementatlon with TmFPS1 in a FPS-deflclent mutant yeast demonstrated that TmFPS1 did encode farnesyl dlphosphate synthase, which rescued the yeast mutant. This study will be helpful In future Investigations aiming at understanding the detailed role of FPS In terpenold biosynthesis flux control at the molecular genetic level.

关键词： farnesyl diphosphate synthase rapid amplification of cDNA ends Taxus media yeast complementation

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Lipomatous ependymoma with ZFTA:RELA fusion-positive:A case report

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World Journal of Clinical Cases 2025年第1期13卷 48-55页

作者： Xiao-Yu Zhao Juan-Han Yu yi-Hua Wang yi-Xin Liu Lu Xu Lin fu Ning yi China Medical University The First Hospital of China Medical UniversityShenyang 110001Liaoning ProvinceChina Department of Pathology The First Hospital of China Medical UniversityShenyang 110001Liaoning ProvinceChina Anshan Cancer Hospital Anshan 114000Liaoning ProvinceChina

BACKGROUND Ependymoma with lipomatous differentiation is a rare type of *** ZFTA fusion-positive supratentorial ependymoma is a novel tumor type in the 2021 World Health Organization classification of central nervous system *** fusion-positive lipomatous ependymoma has not been reported to *** SUMMARY We reported a case of a 15-year-old Chinese male who had a sudden convulsion lasting approximately six *** resonance imaging showed a round cystic shadow of approximately 1.9 cm×1.5 cm×1.9 cm under the right parieto-occipital *** examination showed characteristic perivascular pseudorosettes and adipose differentiation in the ***-chemical staining showed that the tumor cells were negative for cytokeratin,NeuN,Syn and p53,but positive for GFAP,vimentin and S-100 ***-ficant punctate intracytoplasmic EMA immunoreactivity was *** level of Ki-67 was about 5%.Genetic analysis revealed ZFTA:RELA fusion.A cranio-tomy with total excision of the tumor was *** follow-up time was 36 months,no evidence of disease recurrence was found in magnetic resonance *** Based on these findings,the patient was diagnosed as a ependymoma with ZFTA fusion and lipomatous *** case report provides information on the microscopic morphological features of ependymoma with ZFTA fusion and lipomatous differentiation,which can help pathologists to make a definitive diagnosis of this tumor.

关键词： Ependymoma Lipomatous Molecular classification Supratentorial ZFTA:RELA fusion Case report

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Wrinkle analysis of a space planar film reflect-array

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Journal of Zhejiang University-Science A(Applied Physics & Engineering) 2011年第1期12卷 24-32页

作者： Wei-wei XIAO Wu-jun CHEN gong-yi fu Space Structures Research Centre Shanghai Jiao Tong University Shanghai 200030 China

The presence of wrinkles in a membrane is the main factor that induces surface errors on space planar film reflect-arrays. Based on the commercial finite element (FE) package ABAQUS, a numerical procedure for membrane wrinkle analysis was set up, and used to analyze a square planar film reflect-array under pure shear force to evaluate its induced wrinkle characteristics. First, the effect of shear force on the wrinkle pattern of the array was studied and validated by experiment. Second, the effect of prestress was studied. When the prestress increases, the quantity of the wrinkles increases, and the amplitude of the wrinkles decreases. Third, the influence of the boundary conditions was investigated. A frame side edge structure has a relatively smooth surface, but also relatively high stress. Finally, the behavior of a joint seam was analyzed. The results indicate that a joint band has a significant influence on the wrinkle pattern of the membrane.

关键词： Space planar film reflect-array Wrinkle analysis Joint seam

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An approach for shape optimization of stratosphere airships based on multidisciplinary design optimization

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Journal of Zhejiang University-Science A(Applied Physics & Engineering) 2009年第11期10卷 1609-1616页

作者： Ouan-bao WANG Ji-an CHEN gong-yi fu Deng-ping DUAN Institute of Aerospace Science & Technology Shanghai Jiao Tong University Shanghai 200240 China

Airship shape is crucial to the design of stratosphere airships. In this paper, multidisciplinary design optimization (MDO) technology is introduced into the design of airship shape. We devise a composite objective function, based on this technology, which takes account of various factors which influence airship performance, including aerodynamics, structures, energy and weight to determine the optimal airship shape. A shape generation algorithm is proposed and appropriate mathematical models are constructed. Simulation results show that the optimized shape gives an improvement in the value of the composite objective function compared with a reference shape.

关键词： Airship shape Multidisciplinary design optimization (MDO) Adaptive simulation annealing Stratosphere airship

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Soil Respiration in a Subtropical Mangrove Wetland in the Jiulong River Estuary, China

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Pedosphere 2013年第5期23卷 678-685页

作者： JIN Liang LU Chang-yi YE Yong YE gong-fu College of the Environment & Ecology Xiamen University Xiamen University Tan Kah Kee College fujian Institute of Forestry Science

The diurnal and seasonal variations of soil respiration （SR） were studied at a subtropical mangrove wetland in the Jiulong River Estuary from May 2010 to April 2011. SR rates were measured continuously from 08：00 to 06：00 local time （24-h time system） on July 8-9 and October 3-4, 2010; and January 15-16 and April 11-12, 2011. Similar patterns in the diurnal variation of SR were observed on October 2-3 and April 11-12, with the maximum values at 14：00 and the minimum at 00：00. However, the diurnal dynamics of SR on July 8-9, 2010 and January 15-16, 2011 showed different patterns, with the maximum values at 08：00-10：00 on above sampling dates and the minimum at 22：00 on July 8 and at 04：00 on January 16. The daily mean values of SR approximated to the values measured at 08：00. SR fluctuated with distinct seasonal patterns. The seasonal variation was characterized by a mono-peak pattern, with the highest rate （6.18 ~mol CO2 m-2 s-1） in July and the lowest rate （0.36 ~tmol CO2 m-2 s-1） in December. The results showed that the variation of SR in mangrove wetland was mainly controlled by soil temperature, and there was no significant correlation between SR and soil water content. It also implied that the model of SR in mangrove wetland should not only consider the effect of soil temperature, but also incorporate other factors, such as water level, precipitation, microbial activity and photosynthesis, which also could affect SR.

关键词： controlling factors Kandelia candel microbial activity neap tide soil temperature

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Study on Pressure Coefficient Distribution of the Airship Zhiyuan-1

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Journal of Harbin Institute of Technology(New Series) 2013年第3期20卷 86-92页

作者： Ping Liu gong-yi fu Xiao-Liang Wang Qi Song School of Naval Architecture Ocean & Civil EngineeringShanghai Jiaotong University School of Aeronautics and Astronautics Shanghai Jiaotong University China Academy of Space Technology

A wind tunnel tests with different configurations,pitch and yaw angles were performed to study the wind load characteristics of the rigid model of the airship Zhiyuan-1. The rigid model was aimed to simulate a technical demonstrating stratospheric airship named Zhiyuan-1 according to the similarity principle of geometric and Reynolds number. Based on the results of wind tunnel test,the features of pressure coefficient distributions on the surface of the airship were described. It was indicated that the fins and the gondola of airship hardly have the effect on the pressure distribution on the surface of airship,but have obviously effect on the local areas near the fins and the gondola.

关键词： airship hull configuration low-speed wind tunnel test pressure coefficient distribution

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NMDAR-dependent somatic potentiation of synaptic inputs is correlated withβamyloid-mediated neuronal hyperactivity

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Translational Neurodegeneration 2021年第3期10卷 432-446页

作者： yifei Bao Xin Yang yi fu Zhengyan Li Ru gong Wei Lu MOE Key Laboratory of Developmental Genes and Human Disease School of Life Science and TechnologySoutheast UniversityNanjing 210096China Department of Neurology Huashan HospitalInstitute for Translational Brain ResearchState Key Laboratory of Medical NeurobiologyMOE Frontier Center for Brain ScienceFudan UniversityShanghai 200032China Co-Innovation Center of Neuroregeneration Nantong UniversityNantong 226001China

Background:βAmyloid(Aβ)-mediated neuronal hyperactivity,a key feature of the early stage of Alzheimer’s disease(AD),is recently proposed to be initiated by the suppression of glutamate ***,the underlying mechanism by which the impaired glutamate reuptake causes neuronal hyperactivity remains *** suppression of the glutamate reuptake causes accumulation of ambient glutamate that could difuse from synaptic sites at the dendrites to the soma to elevate the tonic activation of somatic N-methyl-D-aspartate receptors(NMDARs).However,less attention has been paid to the potential role of tonic activity change in extrasynaptic glutamate receptors(GluRs)located at the neuronal soma on generation of neuronal ***:Whole-cell patch-clamp recordings were performed on CA1 pyramidal neurons in acute hippocampal slices exposed to TFB-threo-β-benzyloxyaspartic acid(TBOA)or human Aβ_(1-42) peptide oligomer.A series of dendritic patch-clamp recordings were made at diferent distances from the soma to identify the location of the changes in synaptic ***,single-channel recording in the cell-attached mode was performed to investigate the activity changes of single NMDARs at the ***:Blocking glutamate uptake with either TBOA or the human Aβ_(1-42) peptide oligomer elicited potentiation of synaptic inputs in CA1 hippocampal ***,this potentiation specifcally occurred at the soma,depending on the activation of somatic GluN2B-containing NMDARs(GluN2B-NMDARs)and accompanied by a substantial and persistent increment in the open probability of somatic *** the activity of GluN2B-NMDARs at the soma completely reversed both the TBOA-induced or the Aβ_(1-42)-induced somatic potentiation and neuronal ***:The somatic potentiation of synaptic inputs may represent a novel amplifcation mechanism that elevates cell excitability and thus contributes to neuronal hyperactivity initiated by impai

关键词： NMDA receptor Hyperactivity Somatic modifcation Alzheimer’s disease

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Dynamic nanoscale imaging of enriched CO adlayer on Pt(111)confined under h-BN monolayer in ambient pressure atmospheres

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Nano Research 2019年第1期12卷 85-90页

作者： Hao Wu Pengju Ren Peng Zhao Zhongmiao gong Xiaodong Wen yi Cui Qiang fu Xinhe Bao Department of Chemical Physics University of Science and Technology of ChinaHefei 230026China State Key Lab of Catalysis Dalian Institute of Chemical PhysicsChinese Academy of SciencesDalian 116023China National Energy Center for Coal to Clean fuels Synfuels China Co.LtdBeijing 101400China State Key Laboratory of Coal Conversion Institute of Coal ChemistryChinese Academy of SciencesTaiyuan 030001China Vacuum Interconnected Nanotech Workstation Suzhou Institute of Nano-Tech and Nano-BionicsChinese Academy of SciencesSuzhou 215123China

fun dame ntal un derstandi ng of chemistry confined to nano space remains a challe nge since molecules en capsulated in confined microe nviron merits are difficult to be ***,we show that CO adsorptio n on Pt(111)8nfined un der mono layer hexago nal boron nitride(h-BN)can be dynamically imaged using near ambient pressure seanning tunneling microscope(NAP-STM)and thanks to tunneling transparency of the top h-BN *** observed CO superstructures on Pt(111)in different CO atmospheres allow to derive surface coverages of CO adlayers,which are higher in the 8nfined nano space between h-BN and Pt(111)than those on the ope n Pt surface un der the same *** NAP-STM imaging data together with theoretical calculations confirm confinement-induced molecule enrichment effect within the 2D nano space,which reveals new chemistry aroused by the confined nano reactor.

关键词： two-dimensional materials confinement effect near ambient pressure scanning tunneling microscope(NAP-STM) molecule enrichment

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Seasonal changes in tannin and nitrogen contents of Casuarina equisetifolia branchlets

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2009年第2期10卷 103-111页

作者： Li-hua ZHANG gong-fu YE yi-ming LIN Hai-chao ZHOU Qi ZENG MOE Key Lab for Coastal and Wetland Ecosystems School of Life Sciences Xiamen University Xiamen 361005 China fujian Academy of Forestry Fuzhou 350012 China

Seasonal dynamics of total phenolics (TP), extractable condensed tannins (ECT), protein-bound condensed tannins (PBCT), fiber-bound condensed tannins (FBCT), total condensed tannins (TCT), and protein precipitation capacity (PPC) in young, mature and senescent branchlets of Casuarina equisetifolia were studied at Chishan Forestry Center of Dongshan County, fujian Province, China. In addition, nitrogen contents of branchlets at the different developmental stages were also determined. The contents of TP and ECT, and PPC in young branchlets were significantly higher than those in mature and senescent branchlets through the season. However, PBCT contents were significantly higher in senescent branchlets than those in young and mature branchlets; FBCT fluctuated with season. Young branchlets had the highest N content, which decreased during branch maturity and senescence. The highest contents of TP and the lowest contents of TCT and N in young and mature branchlets were observed in summer. There was a significant negative correlation between TP and N contents. In contrast, TCT contents were positively correlated to N contents. Nutrient resorption during senescence and high TCT:N ratios in senescent branchlets are the important nutrient conservation strategies for C. equisetifolia.

关键词： Casuarina equisetifolia Condensed tannins Total phenolics Nitrogen Seasonal dynamics

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