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Erratum to “Analysis of collinear regions of Oryza AA and CC genomes” [Journal of Genetics and Genomics(2009) 3.,667-677]

纪念殷宏章先生百年诞辰暨全国光合作用学术研讨会

作者： Fenhong Hu, jirong huang, Yungang Shen shanghai {3. of plant Physiology and ecology, shanghai institutes for biological sciences, chinese academy of sciences, 3.0 feng lin road, shanghai 2.003.

＜正＞THF1 （Thylakoid Formation 1） is conserved in all oxygenic organisms. The null mutant thf1 exhibits yellow cotyledons, variegated leaves and slower growth rate. The previous studies have shown that THF1 is invo... 详细信息

关键词： Arabidopsis THF1 (Thylakoid Formation 1) PSII complex Stay-green phenotype

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Journal of Genetics and Genomics 2009年第12期36卷 744-744页

作者： Qi feng a,b,Tao huang a,Qiang Zhao a,b,Jingjie Zhu a,b,Zhixin lin a,Bin Han b,a College of Life Science & Biotechnology,{3. Jiao Tong University,{3. 200240,China b National Center for Gene Research & institute of plant Physiology and ecology,{3. institutes of biological sciences,chinese {13. of sciences,{3. 20023.,China College of Life Science & Biotechnology Shanghai Jiao Tong University Shanghai 200240 China National Center for Gene Research & institute of plant Physiology and ecology Shanghai Institutes of Biological Sciences Chinese Academy of Sciences Shanghai 200233 China

In the author line on page 667,the affiliation of the author "Tao huang" was *** the reader’s convenience,the correct affiliation of

关键词： Analysis of collinear regions of Oryza AA and CC genomes Erratum to AA CC

Arabidopsis TT19 Functions as a Carrier to Transport Anthocyanin from the Cytosol to Tonoplasts

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Molecular plant 2012年第2期5卷 387-400页

作者： yi sun Hong Li Ji-Rong huang National Key Laboratory of plant Molecular Genetics Shanghai Institute of Plant Physiology and EcologyShanghai institutes for Biological SciencesChinese Academy of Sciences300 Feng Lin RoadShanghai 200032China

Anthocyanins are synthesized in the cytosolic surface of the endoplasmic reticulum （ER） but dominantly accumulate in the vacuole. Little is known about how anthocyanins are transported from the ER to the vacuole. Here, we provide evidence supporting that Transparent Testa 19 （TT19）, a glutathione 5-transferase （GST）, functions as a carrier to transport cyanidin and/or anthocyanins to the tonoplast. We identified a novel tt19 mutant （tt19-7）, which barely accumulates anthocyanins but produces a 3.% higher level of flavonol than the wild-type （WT）, from ethyl methanesulfonate mutagenized seeds. Expressing TT19-fused green fluorescence protein （GFP） in tt19-7 rescues the mutant phenotype in defective anthocyanin biosynthesis, indicating that TT19-GFP is functional. We further showed that TT19-GFP is localized not only in the cytoplasm and nuclei, but also on the tonoplast. The membrane localization of TT19-GFP was further ascertained by immunoblot analysis. In vitro assay showed that the purified recombinant TT19 increases water solubility of cyanidin （Cya） and cyanidin-3.O-glycoside （C3.）. Compared with C3., Cya can dramatically quench the intrinsic tryptophan fluorescence of TT19 to much lower levels, indicating a higher affinity of TT19 to Cya than to C3.. Isothermal titration calorimetry analysis also confirmed physical interaction between TT19 and C3.. Taken together, our data reveal molecular mechanism underlying TT19-mediated anthocyanin transportation.

关键词： anthocyanin cyanidin transportation TT19 Arabidopsis.

Analysis of collinear regions of Oryza AA and CC genomes

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Journal of Genetics and Genomics 2009年第11期36卷 667-677页

作者： Qi feng Tao huang Qiang Zhao Jingjie Zhu Zhixin lin Bin Han College of Life Science & Biotechnology Shanghai Jiao Tong University Shanghai 200240 China National Center for Gene Research & institute of plant Physiology and ecology Shanghai Institutes of Biological Sciences Chinese Academy of Sciences Shanghai 200233 China

Comparative analyses of genome structure and sequence of closely related species have yielded insights into the evolution and function of plant genomes. A total of 103.844 BAC end sequences delegated -73.8 Mb of O. officinalis that belongs to the CC genome type of the rice genus Oryza were obtained and compared with the genome sequences office cultivar, O. sativa *** cv. Nipponbare. We found that more than 45% of O. officinalis genome consists of repeat sequences, which is higher than that of Nipponbare cultivar. To further investigate the evolutionary divergence of AA and CC genomes, two BAC-contigs of O. officinalis were compared with the collinear genomic regions of Nipponbare. Of 57 genes predicted in the AA genome orthologous regions, 3. had orthologs in the regions of the CC genome. Alignment of the orthologous regions indicated that the CC genome has undergone expansion in both genic and intergenic regions through primarily retroelement insertion. Particularly, the density of RNA transposable elements was 17.95% and 1.78% in O. officinalis and O. sativa, respectively. This explains why the orthologous region is about 100 kb longer in the CC genome in comparison to the AA genome.

关键词： Oryza sativa Oryza officinalis retroelement orthologous

A Crucial Role of the RGS Domain in Trans- Golgi Network Export of AtRGS1 in the Protein Secretory Pathway

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Molecular plant 2013年第6期6卷 1933-1944页

作者： Guangzhen Hu yiping Suo jirong huang National Key Laboratory of plant Molecular Genetics Shanghai Institute of Plant Physiology and Ecology Shanghai Institutes for Biological SciencesChinese Academy of Sciences 300 Feng Lin Road. Shanghai 200032 China Present address: Division of Hematology Department of Internal Medicine Mayo Clinic Rochester MN 55905 USA

The secretory pathway is responsible for the transport of newly synthesized transmembrane proteins from the endoplasmic reticulum to their destinations via the Golgi/trans-Golgi network （TGN）, Cargo proteins at each sta- tion are actively sorted by specific sorting signals on the cargo and the corresponding coat complexes. Here, we used the Arabidopsis regulator of G-protein signaling （AtRGS1）, which contains an N-terminal potentially sensing glucose seven-transmembrane domain and a C-terminal RGS domain, as a model to uncover sorting motifs required for its cell surface expression. Expression of wild-type and truncated or mutated AtRGS1 fluorescent fusion proteins identified two cysteine residues in the extracellular N-terminus that are essential for endoplasmic reticulum exit and/or correct folding of AtRGS1. The linker between the seven-transmembrane and RGS domains contains an endoplasmic reticulum export signal, whereas the C-terminus is dispensable for the plasma membrane expression of AtRGS1. Interestingly, deletion of the RGS domain results in Golgi/TGN localization of the truncated AtRGS1. Further analysis using site-directed mutagen- esis showed that a tyrosine-based motif embedded in the RGS domain is essential for Golgi/TGN export of AtRGS1. These results reveal a new role for the RGS domain in regulating AtRGS1 trafficking from the Golgi/TGN to the plasma membrane and explain the interaction between the seven-transmembrane and RGS domains.

关键词： G-proteins AtRGS1 TGN sorting signal RGS domain Arabidopsis.

Translational Regulation of plant Response to High Temperature by a Dual-Function tRNAHls Guanylyltransferase in Rice

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Molecular plant 2019年第8期12卷 1123-1142页

作者： Ke Chen Tao Guo Xin-Min Li yi-Min Zhang yi-Bing Yang Wang-Wei Ye Nai-Qian Dong Chuan-lin Shi yi Kan You-huang Xiang Hai Zhang Ya-Chao Li Ji-Ping Gao Xuehui huang Qiang Zhao Bin Han Jun-Xiang Shan Hong-Xuan lin National Key Laboratory of plant Molecular Genetics CAS Centre for Excellence in Molecular Plant Sciences and Collaborative Innovation Center of Genetics & DevelopmentShanghai Institute of Plant Physiology & EcologyShanghai Institute for Biological SciencesChinese Academic of SciencesShanghai 200032China University of the chinese academy of sciences Beijing 100049China School of Life Science and Technology ShanghaiTech UniversityShanghai 201210China National Center for Gene Research CAS Center for Excellence in Molecular Plant SciencesShanghai Institute of Plant Physiology and EcologyShanghai Institutes for Biological SciencesChinese Academy of SciencesShanghai 200233China College of Life and Environmental sciences Shanghai Normal UniversityShanghai 200234China

As sessile organisms,plants have evolved numerous strategies to acclimate to changes in environmental ***,the molecular basis of this acclimation remains largely *** this study we identified a tRNAHis guanylyltransferase,AET1,which contributes to the modification of pre-tRNAH,s and is required for normal growth under high-temperature conditions in ***,AET1 possibly interacts with both RACK1A and elF3. in the endoplasmic ***,AET1 can directly bind to OsARF mRNAs including the uORFs of OsARF19 and OsARF23.indicating that AET1 is associated with translation ***,polysome profiling assays suggest that the translational status remains unaffected in the aet1 mutant,but that the translational efficiency of OsARF19 and OsARF23.is reduced;moreover,OsARF23.protein levels are obviously decreased in the aet1 mutant under high temperature,implying that AET1 regulates auxin signaling in response to high *** provide new insights into the molecular mechanisms whereby AET1 regulates the environmental temperature response in rice by playing a dual role in tRNA modification and translational control.

关键词： rice translation regulation tRNA modification auxin signaling environmental temperature

Identification and functional characterization of doublesex gene in the testis of Spodoptera litura

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Insect Science 2019年第6期26卷 1000-1010页

作者： Qian Du Liang Wen Si-Chun Zheng Hong-Lun Bi Yong-Ping huang Qi-Li feng lin Liu Guangzhou Key Laboratory of Insect Development Regulation and Applied Research Institute of Insect Science and Technology School of Life Sciences South China Normal University GuangzhouChina Key Laboratory of Insect Developmental and Evolutionary Biology Institute of Plant Physiology and Ecology Shanghai Institutes for Biological Science Chinese Academy of Sciences ShanghaiChina

Fusion of the testis occurs in most Lepidoptera insects, including Spodoptera litura, an important polyphagous pest. Testicular fusion in S. litura is advantageous for male reproduction, and the molecular mechanism of fusion remains unknown. Doublesex influences the formation of genitalia, the behavior of courtship, and sexually dimorphic traits in fruit-fly and silkworm, and is essential for sexual differentiation. However, its purpose in the testis of S. litura remains unknown. The doublesex gene of S. litura (Sldsx) has male-specific SldsxM and female-specific SldsF isoforms, and exhibits a higher expression level in the male testis. At the testicular fusion stage (L6D6), Sldsx attained the highest expression compared to the pre-fusion and post-fusion periods. Moreover, Sldsx had a higher expression in the peritoneal sheaths of testis than that of germ cells in the follicle. CRISPR/Cas9 (Clustered Regularly Interspaced Short Palindromic Repeats/Cas9) was applied to S. litura to determine the role of Sldsx. A mixture of single guide RNA messenger RNA and Cas9 protein (3.0 ng/μL each) was injected into eggs within 2 h following oviposition. CRISPR/Cas9 successfully induced genomic mutagenesis of Sldsx at Go generation. The mutant males had smaller testis surrounded by less tracheae. Moreover, the mutant males had abnormal external genitalia and could not finish mating with wild-type females. Additionally, testes were fused for almost all mutant males. The results showed that Sldsx was not related to testicular fusion, and is required for both testis development and the formation and function of external genitalia in S. litura. The main roles of doublesex on the male are similar to other insects.

关键词： CRISPR/Cas9 system doublesex external genitalia Spodoptera litura testis

Role of juvenile hormone receptor Methoprene-tolerant 1 in silkworm larval brain development and domestication

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Zoological Research 2021年第5期42卷 637-649页

作者： Yong Cui Zu-Lian Liu Cen-Cen Li Xiang-Min Wei Yong-Jian lin Lang You Zi-Dan Zhu Hui-Min Deng Qi-Li feng Yong-Ping huang Hui Xiang Guangdong Provincial Key Laboratory of Insect Developmental Biology and Applied Technology Guangzhou Key Laboratory of Insect Development Regulation and Application ResearchInstitute of Insect Science and TechnologySchool of Life SciencesSouth China Normal UniversityGuangzhouGuangdong 510631China Key Laboratory of Insect Developmental and Evolutionary Biology Institute of Plant Physiology and EcologyShanghai Institutes for Biological SciencesChinese Academy of SciencesShanghai 200032China College of Life sciences Xinyang Normal UniversityXinyangHenan 464000China

The insect brain is the central part of the neurosecretory system,which controls morphology,physiology,and behavior during the insect’s *** are holometabolous insects,and their brains develop during the larval period and metamorphosis into the adult *** the only fully domesticated insect,the Lepidoptera silkworm Bombyx mori experienced changes in larval brain morphology and certain behaviors during the domestication *** regulation in insects is a key factor in multiple ***,how juvenile hormone(JH)signals regulate brain development in Lepidoptera species,especially in the larval stage,remains *** recently identified the JH receptor Methoprene tolerant 1(Met1)as a putative domestication *** artificial selection on Met1 impacts brain and behavioral domestication is another important issue addressing Darwin’s theory on ***,CRISPR/Cas9-mediated knockout of Bombyx Met1 caused developmental retardation in the brain,unlike precocious pupation of the *** the whole transcriptomelevel,theecdysteroid(20-hydroxyecdysone,20 E)signaling and downstream pathways were overactivated in the mutant cuticle but not in the *** related to cell proliferation and specialization processes,such as extracellular matrix(ECM)-receptor interaction and tyrosine metabolism pathways,were suppressed in the *** evolutionary analysis and in vitro assay identified an amino acid replacement located in a novel motif under positive selection in ***,which decreased transcriptional binding *** *** MET1 protein showed a changed structure and dynamic features,as well as a weakened co-expression gene network,compared with *** on comparative transcriptomic analyses,we proposed a pathway downstream of JH signaling(i.e.,tyrosine metabolism pathway)that likely contributed to silkworm larval brain development and domestication and highlighted the importance of the biogenic amine syste

关键词： Met1 Brain Artificial selection Tyrosine metabolism pathway Silkworm

The Genome of Medicinal plant Macleaya cordata Provides New Insights into Benzylisoquinoline Alkaloids Metabolism

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Molecular plant 2017年第7期10卷 975-989页

作者： Xiubin Liu yisong Liu Peng huang Yongshuo Ma Zhixing Qing Qi Tang Huifen Cao Pi Cheng Yajie Zheng Zejun Yuan Yuan Zhou Jinfeng Liu Zhaoshan Tang yixiu Zhuo Yancong Zhang linlan Yu Jialu huang Peng Yang Qiong Peng dinbo Zhang Wenkai Jiang Zhonghua Zhang Kui lin Dae-Kyun Ro Xiaoya Chen Xingyao Xiong yi Shang Sanwen huang Jianguo Zeng National and Local Union Engineering Research Center of Veterinary Herbal Medicine Resource and Initiative Hunan Co-Innovation Center for Utilization of Botanical Functional Ingredients Horticulture and Landscape College Veterinary Medicine College Hunan Agricultural University Changsha 410128 China institute of Vegetables and Flowers Chinese Academy of Agricultural Sciences Key Laboratory of Biology and Genetic Improvement of Horticultural Crops of the Ministry of Agriculture Sino-Dutch Joint Laboratory of Horticultural Genomics Beijing 100081 China Agricuitural Genome institute at Shenzhen Chinese Academy of Agricultural Sciences Shenzhen 518124 China School of Pharmacy Hunan University of Chinese Medicine Changsha 410208 China MOE Key Laboratory for Biodiversity Science and Ecological Engineering and College of Life sciences Beijing Normal University Beijing 100875 China Micolta Bioresource Inc. Changsha 410016 China Herbal Extract Engineering Research Center Changsha 410331 China Biotechnology Research Center Hunan Academy of Agricultural Sciences Changsha 410125 China Novogene Bioinformatics institute Beijing 100083 China Department of biological sciences University of Calgary Calgary T2N1N4 Canada National Key Laboratory of plant Molecular Genetics National Plant Gene Research Center Institute of Plant Physiology and Ecology Shanghai Institutes forBiological Sciences Chinese Academy of Sciences Shanghai 200032 China plant Science Research Center Shanghai Chenshan Botanical Garden Shanghai 201602 China

The overuse of antibiotics in animal agriculture and medicine has caused a series of potential threats to public health. Macleaya cordata is a medicinal plant species from the Papaveraceae family, providing a safe resource for the manufacture of antimicrobial feed additive for livestock. The active constituents from M. cordata are known to include benzylisoquinoline alkaloids （BIAs） such as sanguinarine （SAN） and chelerythrine （CHE）, but their metabolic pathways have yet to be studied in this non-model plant. The active biosynthesis of SAN and CHE in M. cordata was first examined and confirmed by feeding ^13.-labeled tyrosine. To gain further insights, we de novo sequenced the whole genome of M. cordata, the first to be sequenced from the Papaveraceae family. The M. cordata genome covering 3.8 Mb encodes 22,3.8 predicted protein-coding genes with 43.5% being transposable elements. As a member of basal eudicot, M. cordata genome lacks the paleohexaploidy event that occurred in almost all eudicots. From the genomics data, a complete set of 16 metabolic genes for SAN and CHE biosynthesis was retrieved, and 14 of their biochemical activities were validated. These genomics and metabolic data show the conserved BIA metabolic pathways in M. cordata and provide the knowledge foundation for future productions of SAN and CHE by crop improvement or microbial pathway reconstruction.

关键词： Macleaya cordata genome Benzylisoquinoline alkaloids Secondary metabolism Sanguinarine Chelerythrine Isotopic labeling