检索结果-南通市图书馆

维普期刊数据库

Carpel of cucumber (Cucumis sativus. L) male flowers maintains early primordia characteristics during organ development

在线全文

学校读者我要写书评

暂无评论

Chinese Science Bulletin 2000年第8期45卷 729-733页

作者： YANG Lingling CHEN Min LIU Fuquan geng yi CHEN Chong LI yiqin CAO Zongxun XU Zhihong BAI Shunong Department of Biological Sciences and Biotechnology Tsinghua UniversityBeijing 100084China College of Life Sciences Peking UniversityBeijing 10871China Shanghai Institute of Plant Physiology CASShanghai 200032China

Diclinism is not only an interesting natural phenomenon in plant kingdom, but also of significance in the yield of crops, e.g. cucumber and watermelon. Cucumber has been a model plant for studying the diclinism for decades. However, it is stili obscure how the carpel primordia of cucumber male flower undergo the developmental processes after the initiation of their development. By showing morphological alternation, metabolic activity, as well as CFL gene expression, the present study demonstrated that the carpel primordia of cucumber male flowers maintained the characteristics of primordium until anthesis, although it developed significantly slower than that of the female flowers.

关键词： cucumber dilinism primordium carpel development.

维普期刊数据库

Copper-Free Click Chemistry-Mediated Assembly of Single Quantum Dot Nanosensor for Accurately Monitoring Locus-Specific m^(6)A in Cancer Cells

在线全文

学校读者我要写书评

暂无评论

Chinese Journal of Chemistry 2024年第5期42卷 499-504页

作者： Yue-ying Li Ning-Ning Zhao yi-Xuan geng Qian Han Jian-Ge Qiu Bing-Hua Jiang Zi-Yue Wang Chun-Yang Zhang Translational Medicine Center The First Affiliated Hospital of Zhengzhou UniversityThe Academy of Medical SciencesHenan Institute of Medical and Pharmaceutical Sciences BGI CollegeZhengzhou UniversityZhengzhouHenan450052 China College of Chemistry Chemical Engineering and Materials ScienceShandong Normal UniversityJinanShandong250014 China School of Chemistry and Chemical Engineering Southeast UniversityNanjingJiangsu211189 China

N6-methyladenosine(m^(6)A)plays an important role in embryogenesis,nuclear export,transcription splicing,and protein translation ***,we demonstrate a copper-free click chemistry-mediated assembly of single quantum dot(QD)nanosensor for accurately monitoring locus-specific m^(6)A in cancer *** m^(6)A-sensitive endoribonuclease MazF can digest the unmethylated A-RNA,and the intact m^(6)A-RNA then hybridizes with DNA probes a and b to produce a sandwich hybrid,initiating the click chemistry to generate probe a–b ligation product via first tandem ligation detection reaction(LDR)***,DNA probes c and d can hybridize with the probe a–b ligation product to generate the probe c–d ligation product via second LDR *** LDR cycles can be repeated through denaturation and annealing reaction to generate abundant biotin-/fluorophore-modified probe c–d ligation products that can easily assemble on the QD surface to induce distinct fluorescence resonance energy transfer(FRET)between QD and *** assay can be homogenously performed without the involvement of copper catalyst,m^(6)A-specific antibody,radioactive labeling,ligase enzyme,enzymatic reverse transcription,and next-generation ***,it can discriminate even 0.01% m^(6)A level in complex samples and accurately measure cellular m^(6)A-RNA expression,providing a promising avenue for clinical diagnostics and biomedical research.

关键词： RNA methylation Click chemistry Förster resonance energy transfer Biosensor Cancer

Annulare With Tuberculosis and Cutis Laxa: A Rare Case Report

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

International Journal of Dermatology and Venereology 2023年第3期6卷 172-174页

作者： yi-Fei Wang yi geng Xiu-Lian Xu Department of Pathology Hospital for Skin Diseases(Institute of Dermatology)Chinese Academy of Medical Sciences and Peking Union Medical CollegeNanjingJiangsu 210042China

Introduction:Granuloma annulare(GA)is a benign,noninfectious granulomatous disorder of unknown etiology,characterized by an annular arrangement of erythematous small nodules and plaques,classically localized on dorsal surfaces of the hands and *** shows palisading granulomas with a central zone of necrobiotic collagen and mucin deposition surrounded by a palisade of ***,we reported a 24-year-old man with GA combined with tuberculosis and cutis *** presentation:A 24-year-old man was presented with skin annular lesions on the trunk and extremities without subjective symptoms for three *** annular lesions first arose in the trunk that slowly progressed to most parts of the body and cutis laxa-like lesions appeared on the left inguinal region with inguinal hernia two years *** the same time,the patient was diagnosed with pulmonary tuberculosis due to the hemoptysis for two years and the sputum culture for tuberculosis bacillus was *** was treated with triple anti-tuberculosis drugs *** remission of the skin lesions was noted since *** examination showed similar histological changes in the interstitial pattern of ***:Tuberculosis was a possible etiologic factor in GA because of the coincidental occurrence,granulomatous histologic features,and positive tuberculin skin *** fact,some cases of GA with chronic pulmonary tuberculosis responding to a specific anti-tuberculosis therapy were *** is worth noting that tuberculids,generalized exanthems in the skin of tuberculous patients possibly resulting from hypersensitivity reactions to the tubercle bacillus,may mimic ***,our patient also showed extremely characteristic slack skin in posterior lumbar and groin,which was deemed to be related to reduction and destruction of elastic ***:Generalized interstitial GA with tuberculosis and cutis laxa is vary rare,which is worth being aware of the possibility of such a occ

关键词： case report cutis laxa interstitial granuloma annulare

维普期刊数据库

THE TINY PAINTINGS OF THE BUDDHIST WORLD——A RECORD OF LIU ENJUN AND HIS TSAKA PAINTINGS

在线全文

学校读者我要写书评

暂无评论

China's Tibet 2010年第6期21卷 50-61页

作者： Chen Dan Liu Enjun & geng yi

EDITOR’S COMMENTARY This special column consists of devoted fans of Tibetan culture. They have spent their life,their energy and their finances to eagerly collect and preserve unique Tibetan traditional culture while virtually also enjoying the spiritual and material happiness which those treasures bring to *** now on,this journal is going to invite Chen Dan to contribute her detailed and delicate stories about collectors of Tibetan artifacts one by one,

关键词：绘画微型佛教油画民族传统文化藏族文化

Expression of SNC73, a transcript of the immunoglobulin α-1 gene, in human epithelial carcinomas

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

World Journal of Gastroenterology 2007年第16期13卷 2305-2311页

作者： Li-yi geng Zheng-Zhen Shi Qi Dong Xin-Han Cai Yan-Ming Zhang Wei Cao Jia-Ping Peng Yong-Ming Fang Lei Zheng Shu Zheng Cancer Institute Zhejiang University School of Medicine Hangzhou 310009 Zhejiang Province China

AIM： To investigate the expression of SNC73, a transcript of the immunoglobulin α-1 gene （IgA1-H chain）, in human epitheliα-derived tumor cells. METHODS： Total RNAs and cell lysates were prepared from five different human epithelial cell lines derived from lung, stomach, liver, skin, and breast, respectively. RT-PCR and immunoblot analysis of these five cell lines were done. Both RT-PCR and immunochemistry were used to detect the expression of SNC73 in these cell lines. We also examined the expression of SNC73 in normal epithelial cells of colon mucosa by in situ hybridization. RT-PCR and immunoblot analysis were used to determine whether the recombination activating gene1/2 （RAG1 and RAG2） is present. The expression of three immunoglobulin transcription factors, EBF, E2A and Pax5, and the heavy chain of IgA1 and two types of light chains of immunoglobulin （κ and λ） in the aforementioned cell lines were analyzed by RT-PCR and immunochemistry, respectively. All the RT-PCR products were analyzed by sequencing. RESULTS： The results of RT-PCR and immunochemistry showed that both mRNA and protein of SNC73 were expressed in five human epitheliα-derived cancer cell lines. These data were further confirmed in the normal epithelial cells of colon mucosa by in situ hybridization. Also, the heavy chain of IgA1 and κ light chain were detected in these cells, but no λ light chain was observed. Both RAG1 and RAG2 were expressed in these human epitheliα-derived cancer cell lines and the sequence was identical to that expressed in pre-B and pre-T cells. In addition to RAG1 and RAG2, the mRNA in one of the immunoglobulin transcription factors, EBF, was also detected in these cell lines, and Pax5 was only expressed in SW480 cells, but no expression of E2A was observed in all the five cell lines. CONCLUSION： Immunoglobulin A1 is originally expressed and V（D）J recombination machine is also present in non-lymphoid cells, suggesting that V（D）J recombination machine mediates the assembly

关键词： SNC73 Immunoglobulin A1 Epithelial cancer cells Recombination activating gene1/2 Immunoglobulin transcription factor

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

p53活性四聚体全原子分子动力学分析

物理学报 2024年第4期73卷 300-313页

作者：周晗耿轶钊晏世伟北京师范大学物理学系北京100875 河北工业大学理学院天津300131 北京师范大学文理学院珠海519085

p53是一种肿瘤抑制蛋白,对阻碍癌症发展、维持遗传完整性起着至关重要的作用.在细胞核内,4个p53分子通过高度协同的方式、通过DNA结合域与DNA结合,形成稳定的四聚体活性结构,并转录激活或抑制其靶向基因.然而,大多数肿瘤细胞中存在大量... 详细信息

p53是一种肿瘤抑制蛋白,对阻碍癌症发展、维持遗传完整性起着至关重要的作用.在细胞核内,4个p53分子通过高度协同的方式、通过DNA结合域与DNA结合,形成稳定的四聚体活性结构,并转录激活或抑制其靶向基因.然而,大多数肿瘤细胞中存在大量p53的突变,其中绝大部分突变发生在p53的DNA结合域,而p53的DNA结合域又是p53形成四聚体活性结构、调控下游靶基因转录的重要区域.本文通过全原子分子动力学模拟,研究了野生型p53四聚体内分子间的相互作用机制.结果表明,位于DNA两侧的对称二聚体是一个稳定的二聚体,在与DNA结合前后都能维持稳定的结构.位于DNA同侧的两个单体依靠两个接触面提供的蛋白-蛋白相互作用和DNA的骨架作用使四聚体活性结构保持稳定,这些相互作用为四聚体的形成机制提供了重要支撑.该工作厘清了p53四聚体在动力学过程中的内部相互作用机制和关键残基,揭示了四聚化过程中各个相互作用界面的关键位点,对于理解p53的抑癌机制、探索有效治癌策略、发展治癌药物具有重要意义.

关键词： p53核心四聚体 p53核心结构域蛋白-蛋白相互作用分子动力学模拟

维普期刊数据库博看期刊

Nutlin-3a对p53-MDM2复合物稳定性影响

在线全文

学校读者我要写书评

暂无评论

物理学报 2023年第6期72卷 359-366页

作者：周晗耿轶钊晏世伟北京师范大学物理学系北京100875 河北工业大学理学院天津300131

p53蛋白是一种与细胞周期停滞和细胞凋亡有关的蛋白质.在受到细胞压力或环境扰动后,p53促进下游多个靶基因的转录,介导肿瘤抑制.MDM2是主要的E3泛素连接酶,也是p53的负调控因子.MDM2可促进p53的泛素化和核输出,抑制p53的抑癌活性.因此M... 详细信息

p53蛋白是一种与细胞周期停滞和细胞凋亡有关的蛋白质.在受到细胞压力或环境扰动后,p53促进下游多个靶基因的转录,介导肿瘤抑制.MDM2是主要的E3泛素连接酶,也是p53的负调控因子.MDM2可促进p53的泛素化和核输出,抑制p53的抑癌活性.因此MDM2对p53的负调控始终是肿瘤治疗中急切需要解决的问题.Nutlin-3a是被证明可以有效抑制p53-MDM2相互作用的小分子抑制剂.本文使用全原子分子动力学模拟,研究Nutlin-3a对p53-MDM2复合物的稳定性的影响.结果表明,通过引起p53和MDM2间Phe19-Gln72的氢键和Glu17-Lys94的盐桥发生的断裂,Nutlin-3a可以削弱p53和MDM2间的相互作用.我们的工作对Nutlin-3a小分子抑制剂的作用机制进行了说明,揭示了抗癌药物Nutlin-3a介导的p53-MDM2复合物亲和力降低的分子机制,并为针对p53蛋白的有效抗癌治疗提供了理论基础.

关键词：治癌药物设计 p53-MDM2复合物 Nutlin-3a抑制剂分子分子动力学模拟

维普期刊数据库博看期刊