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Rapid fabrication of modular 3D paper-basedmicrofluidic chips using projection-based 3D printing

Bio-Design and Manufacturing 2024年第5期7卷 611-623页

作者： mingjun Xie Zexin Fu Chunfei Lu Sufan Wu Lei Pan Yong He yi Sun ji Wang Plastic and Reconstructive Surgery Center Department of Plastic and Reconstructive SurgeryZhejiang Provincial People’s HospitalAffiliated People’s HospitalHangzhou Medical CollegeHangzhou 310014China State Key Laboratory of Fluid Power and Mechatronic Systems School of Mechanical EngineeringZhejiang UniversityHangzhou 310058China Key Laboratory of Materials Processing and Mold Zhengzhou UniversityZhengzhou 450002China Key Laboratory of 3D Printing Process and Equipment of Zhejiang Province College of Mechanical EngineeringZhejiang UniversityHangzhou 310058China Cancer Center Zhejiang UniversityHangzhou 310058China

Paper-based microchips have different advantages,such as better biocompatibility,simple production,and easy handling,making them promising candidates for clinical diagnosis and other *** study describes amethod developed to fabricate modular three-dimensional(3D)paper-based microfluidic chips based on projection-based 3D printing(PBP)technology.A series of two-dimensional(2D)paper-based microfluidic modules was designed and *** evaluating the effect of exposure time on the accuracy of the flow channel,the resolution of this channel was experimentally ***,several 3D paper-based microfluidic chips were assembled based on the 2D ones using different methods,with good channel ***-based 2D and hydrogel-based 3D cell culture systems based on 3D paper-based microfluidic chips were verified to be ***,by combining extrusion 3D bioprinting technology and the proposed 3D paper-based microfluidic chips,multiorgan microfluidic chips were established by directly printing 3D hydrogel structures on 3D paperbased microfluidic chips,confirming that the prepared modular 3D paper-based microfluidic chip is potentially applicable in various biomedical applications.

关键词： Paper-based microfluidic chip Projection-based 3D printing(PBP) Modularization Cell culture

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A novel transcription factor FnMYB4 regulates pigments metabolism of yellow leaf mutants in Fragaria nilgerrensis

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Horticultural Plant Journal 2024年第5期10卷 1134-1150页

作者： Shu jiang yi ji jingyu Yue mingqian Wang Yumeifeng jia Li Xue jiajun Lei College of Horticulture Shenyang Agricultural UniversityShenyangLiaoning 110866China

The strawberry species Fragaria nilgerrensis Schlechtendal ex ***,renowned for its distinctive white,fragrant peach-like fruits and strong disease resistance,is an exceptional research *** a previous study,an ethyl methane sulfonate(EMS)mutant library was established for this species,resulting in various yellow leaf *** yellowing materials are not only the ideal materials for basic studies on photosynthesis mechanism,chloroplast development,and molecular regulation of various pigments,but also have important utilization value in ornamental plants *** present study focused on four distinct yellow leaf mutants:mottled yellow leaf(MO),yellow green leaf(YG),light green leaf(LG),and buddha light leaf(BU).The results revealed that the flavonoid content and carotenoid-to-chlorophyll ratio exhibited a significant increase among these mutants,while experiencing a significant decrease in chlorophyll and carotenoid contents compared to the wild type(WT).To clarify the regulatory mechanisms and network relationships underlying these mutants,the RNA-seq and weighted gene coexpression network(WGCNA)analyses were *** results showed flavonoid metabolism pathway was enriched both in MO and YG mutants,while the chlorophyll biosynthesis pathway and carotenoid degradation pathway were only enriched in MO and YG mutants,***,key structural genes and transcription factors were identified on metabolic pathways of three pigments through correlation analyses and quantitative ***,a R2R3-MYB transcription factor,FnMYB4,was confirmed to be positively correlated with flavonoid synthesis through transient overexpression,virus-induced gene silencing(VIGS),and RNA interference(RNAi),accompanying by reoccurrence and attenuation of mutant ***,dual-luciferase(LUC)and yeast one-hybrid assays confirmed the binding of FnMYB4 to the FnFLS and FnF3H promoters,indicating that FnMYB4 positively regulates flavonoid sy

关键词： Fragaria nilgerrensis Mutant Leaf yellowing RNA-seq Flavonoid MYB

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NLRP3 activation in macrophages promotes acute intestinal injury in neonatal necrotizing enterocolitis

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World Journal of Pediatrics 2024年第2期20卷 153-164页

作者： Bo Shi Cheng-jie Lyu Zhen-Kai Le Hao-Sen ji yi Xiao Yuan-Yuan Zhang Shou-jiang Huang Lin-Jun Yu Qiang Shu jin-Fa Tou Deng-ming Lai Department of Neonatal Surgery Children’s HospitalZhejiang University School of MedicineNational Clinical Research Center for Child HealthHangzhou 310052China Department of Pulmonology Children’s HospitalZhejiang University School of MedicineNational Clinical Research Center for Child HealthHangzhou 310052China Department of Thoracic and Cardiovascular Surgery Children’s HospitalZhejiang University School of MedicineNational Clinical Research Center for Child HealthHangzhou 310052China

Background Macrophages are involved in various immune inflammatory disease *** study aimed to investigate the role and mechanism of macrophages in regulating acute intestinal injury in neonatal necrotizing enterocolitis(NEC).Methods CD68,nucleotide-binding oligomerization domain,leucine-rich repeat,and pyrin domain-containing 3(NLRP3),cysteine aspartate-specific protease-1(caspase-1),and interleukin-1β(IL-1β)in paraffin sections of intestinal tissues from NEC and control patients were detected with immunohistochemistry,immunofluorescence,and western *** pet milk,hypoxia and cold stimulation were used to establish a mouse(wild type and Nlrp3^(-/-))model of *** mouse macrophage(RAW 264.7)and rat intestinal epithelial cell-6 lines were also cultured followed by various ***,intestinal epithelial cell injuries,and IL-1β release were *** Compared to the gut“healthy”patients,the intestinal lamina propria of NEC patients had high macrophage infiltration and high NLRP3,caspase-1,and IL-1β ***,in vivo,the survival rate of Nlrp3^(-/-)NEC mice was dramatically improved,the proportion of intestinal macrophages was reduced,and intestinal injury was decreased compared to those of wild-type NEC ***3,caspase-1,and IL-1β derived from macrophages or supernatant from cocultures of macrophages and intestinal epithelial cells also caused intestinal epithelial cell *** Macrophage activation may be essential for NEC ***3/caspase-1/IL-1β cellular signals derived from macrophages may be the underlying mechanism of NEC development,and all these may be therapeutic targets for developing treatments for NEC.

关键词： Caspase-1 Interleukin-1β Macrophage Necrotizing enterocolitis NLRP3

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Measurement of the neutron-induced total cross sections of ^(nat)Pb from 0.3 eV to 20 MeV on the Back-n at CSNS

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Nuclear Science and Techniques 2024年第2期35卷 85-93页

作者： jie-ming Xue Song Feng Yong-Hao Chen Han yi Min Xiao Pin-jing Cheng Xin-Xiang Li Rong Liu yi-Wei Yang Zi-jie Han Da-Jun Zhao Hao-Qiang Wang Bao-Qian Li ji-Rong Zhao Lei-Xun Tang Wen Luo Bo Zheng School of Nuclear Science and Technology University of South ChinaHengyang 421001China Institute of High Energy Physics Chinese Academy of Sciences(CAS)Beijing 100049China Spallation Neutron Source Science Center Dongguan 523803China Institute of Nuclear Physics and Chemistry China Academy of Engineering PhysicsMianyang 621900China

The neutron-induced total cross sections of natural lead have been measured in a wide energy range(0.3 eV-20 MeV)on the back-streaming white neutron beamline(Back-n)at the China Spallation Neutron *** energy was determined by the neutron total cross-section spectrometer using the time-of-flight technique.A fast multi-cell fission chamber was used as the neutron detector,and a 10-mm-thick high-purity natural lead sample was employed for the neutron transmission *** on-beam background was determined using Co,In,Ag,and Cd *** excitation function of ^(nat)Pb(n,tot)reaction below 20 MeV was calculated using the TALYS-1.96 nuclear-reaction modeling *** present results were compared with previous results,the evaluated data available in the five major evaluated nuclear data libraries(i.e.,ENDF/B-VIII.0,JEFF-3.3,JENDL-5,CENDL-3.2,and BROND-3.1),and the theoretical calculation *** agreement was found between the new results and those of previous experiments and with the theoretical curves in the corresponding *** measurement obtained the neutron total cross section of natural lead with good accuracy over a wide energy range and added experimental data in the resonance energy *** provides more reliable experimental data for nuclear engineering design and nuclear data evaluation of lead.

关键词： Neutron-induced total cross section Natural lead Time-of-flight TALYS-1.96 CSNS Back-n white neutron beamline

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Probing the interaction between asphaltene-wax and its effects on the crystallization behavior of waxes in heavy oil via molecular dynamics simulation

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Petroleum Science 2024年第4期21卷 2839-2848页

作者： Yong Hu Xi Lu Hai-Bo Wang ji-Chao Fang yi-Ning Wu jian Fang Sun SINOPEC Petroleum Exploration and Production Research Institute Shandong Key Laboratory of Oilfield Chemistry School of Petroleum Engineering China University of Petroleum (East China)

High content of asphaltenes and waxes leads to the high pour point and the poor flowability of heavy oil,which is adverse to its efficient development and its transportation in *** the interaction mechanism between asphaltene-wax is crucial to solve these problems,but it is still *** this paper,molecular dynamics simulation was used to investigate the interaction between asphaltenewax and its effects on the crystallization behavior of waxes in heavy *** show that molecules in pure wax are arranged in a paralleled *** wax molecules in heavy oil,which are close to the surface of asphaltene aggregates,are bent and arranged *** the mass fraction of asphaltenes in asphaltene-wax system(ωasp) is 0-25 wt%,the attraction among wax molecules decreases and the bend degree of wax molecules increases with the increase of ω*** ωaspincreases from 0 to 25 wt%,and the attraction between asphaltene-wax is stronger than that among *** causes that the wax precipitation point changes from 353 to 333 *** the ωaspincreases to 50 wt%,wax molecules are more dispersed owing to the steric hindrance of asphaltene aggregates,and the interaction among wax molecules transforms from attraction to *** causes that the ordered crystal structure of waxes can't be formed at normal ***,the asphaltene,with the higher molecular weight or the more hetero atoms,has more obvious inhibition to the formation of wax ***,resins also have an obvious inhibition on the wax crystal due to the formation of asphalteneresin aggregates with a larger *** results reveal the interaction mechanism between asphaltene-wax,and provide useful guidelines for the development of heavy oil.

关键词： Heavy oil Interaction mechanism Asphaltenes Waxes Molecular dynamics

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Movement of ACC oxidase 3 mRNA from seeds to flesh promotes fruit ripening in apple

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Molecular Plant 2024年第8期17卷 1221-1235页

作者： Ting Wang yi Zheng Chen Xu Yulin Deng Xinyi Hao Zicheng Chu ji Tian yi Wang Xinzhong Zhang Zhenhai Han Ting Wu College of Horticulture China Agricultural UniversityBeijingChina Plant Science and Technology College Bioinformatics CenterBeijing University of AgricultureBeijingChina

Xenia,the phenomenon in which the pollen genotype directly affects the phenotypic characteristics of maternal tissues(i.e.,fruit ripening),has applications in crop production and ***,the underlying molecular mechanism has yet to be ***,we investigated whether mobile mRNAs from the pollen affect the ripening and quality-related characteristics of the fruit using cross-pollination between distinct Malus domestica(apple)*** demonstrated that hundreds of mobile mRNAs originating fromthe seeds are deliveredto *** themovementof one of these mRNAs,AcC oxidase 3(MdAco3),is coordinated with fruit *** acid treatment,which can cause plasmodesmal closure,blocks MdAcO3 movement,indicating that MdAcO3 transcripts may move through the *** assess the role of mobile MdAcO3 transcripts in apple fruit,we created MdACO3-GFP-expressing apple seeds using MdACO3-GFP-overexpressing pollen for pollination and showed that MdACO3 transcripts in the transgenic seeds move to the flesh,where they promote fruit ***,we demonstrated that MdAco3 can be transported from the seeds to fruit in the fleshy-fruited species tomato and *** results underscore the potential of mobile mRNAs from seeds to influence fruit characteristics,providing an explanation for the xenia ***,our findings highlight the feasibility of leveraging diverse pollen genomic resources,without resorting to genome editing,to improve fruitquality.

关键词： fruit ripening xenia ACO3 mobile RNA seed(to flesh)

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Real-World Evidence of Multiple Air Pollutants and Mortality:A Prospective Cohort Study in an Oldest-Old Population

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Environment & Health 2024年第1期2卷 23-33页

作者： Linxin Liu yi Zeng John S.ji Vanke School of Public Health and School of Medicine Tsinghua UniversityBeijingChina 100084 Center for the Study of Aging and Human Development School of MedicineDuke UniversityDurhamNorth Carolina 27710United States Center for Healthy Aging and Development Studies National School of DevelopmentPeking UniversityBeijingChina 100091 Vanke School of Public Health Tsinghua UniversityBeijingChina 100084

We aimed to report real-world longitudinal ambient air pollutants levels compared to WHO Air Quality Guidelines(AQG)and analyze multiple air pollutants’joint effect on longevity,and the modification and confounding from the climate and urbanization with a focus on the *** study included 13,207 old participants with 73.3%aged 80 and beyond,followed up from 2008 to 2018 in 23 Chinese *** used the Cox-proportional hazards model and quantile-based g-computation model to measure separate and joint effects of the multiple *** adjusted for climate and area economic factors based on a directed acyclic *** 2018,no participants met the WHO AQG for PM_(2.5) and O_(3),and about one-third met the AQG for NO_(2).The hazard ratio(HR)for mortality was 1.07(95%confidence interval-CI:1.05,1.09)per decile increase in all three pollutants,with PM_(2.5) being the dominant contributor according to the quantile-based g-computation *** the three-pollutant model,the HRs(95%CI)for PM_(2.5) and NO_(2) were 1.27(1.25,1.3)and 1.08(1.05,1.12)per 10μg/m3 increase,*** oldest-old experienced a much lower mortality risk from air pollution compared to the *** mortality risk of PM_(2.5) was higher in areas with higher annual average *** adjustment of road density considerably intensified the association between NO_(2) and *** ambient PM_(2.5) and O_(3) levels in China exceeded the WHO AQG target *** pollutants coexposure,confounding,and modification of the district economic and climate factors should not be ignored in the association between air pollution and mortality.

关键词： air quality multiple exposures climate district economic health equity

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Efficacy and safety of remimazolam in bronchoscopic sedation:A meta-analysis

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World Journal of Clinical Cases 2024年第6期12卷 1120-1129页

作者： ying Zhou Cheng Zhao yi-Xun Tang ji-Tong Liu Department of Anesthesiology The First Affiliated Hospital of Hunan Normal University(Hunan Provincial People’s Hospital)Changsha 410005Hunan ProvinceChina Department of Anesthesiology Zhangjiajie People's HospitalZhangjiajie 427000Hunan ProvinceChina

BACKGROUND Remimazolam is a new benzodiazepine used for procedural sedation and general *** studies have used remimazolam for bendable *** To assess the safety and efficacy of remimazolam for sedation in patients undergoing bendable bronchoscopy by performing a meta-analysis of randomized controlled trials(RCTs).METHODS We searched the EMBASE,PubMed,Cochrane Library,and Web of Science databases for RCTs on bendable bronchoscopic procedural sedation with remimazolam vs conventional sedatives(CS).RESULTS Five studies with 1080 cases were *** had the same sedation success rate compared with CS[relative risk(RR):1.35,95%CI:0.60-3.05,P=0.474,I2=99.6%].However,remimazolam was associated with a lower incidence of hypotension(RR:0.61;95%CI:0.40-0.95,P=0.027;I2=65.1%)and a lower incidence of respiratory depression(RR:0.50,95%CI:0.33-0.77,P=0.002,I2=42.3%).A subgroup analysis showed a higher success rate of sedation with remimazolam than midazolam(RR:2.45,95%CI:1.76-3.42,P<0.001).Compared with propofol,the incidence of hypotension(RR:0.45,95%CI:0.32-0.64,P<0.001,I2=0.0%),respiratory depression(RR:0.48,95%CI:0.30-0.76,P=0.002,I2=78.4%),hypoxemia(RR:0.36,95%CI:0.15-0.87,P=0.023),and injection pain(RR:0.04,95%CI:0.01-0.28,P=0.001)were *** Remimazolam is safe and effective during *** sedation success rate was similar to that in the CS ***,remimazolam has a higher safety profile,with fewer inhibitory effects on respiration and circulation.

关键词： Remimazolam Bronchoscopy Procedural sedation Meta-analysis

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儿童行为抑制测验及行为训练

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中国儿童保健杂志 2020年第5期28卷 493-496页

作者：魏书珍衣明纪青岛大学附属医院儿童保健科山东青岛266003

行为抑制是执行功能的核心内容,是为完成任务而对无关信息或行为的抑制。其早期缺陷易导致社会性交流障碍,工作记忆、情绪调节、注意力等执行功能问题。随着对行为抑制认识的逐渐深入,出现了一系列成熟的测验方法,包括Stroop任务、侧抑... 详细信息

行为抑制是执行功能的核心内容,是为完成任务而对无关信息或行为的抑制。其早期缺陷易导致社会性交流障碍,工作记忆、情绪调节、注意力等执行功能问题。随着对行为抑制认识的逐渐深入,出现了一系列成熟的测验方法,包括Stroop任务、侧抑制、Navon任务、Simon任务以及多源冲突任务等,这些行为抑制测验方法在行为障碍的诊断中发挥了重要作用。对正在发育的儿童实施行为抑制训练可促进执行功能发展。行为抑制的早期健康发展可有效提高儿童的自控力和注意力,从而改善学习能力。了解有关行为抑制的测验方法及行为训练,对促进儿童执行功能发展有重要作用。

关键词：行为抑制儿童执行功能行为训练

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Spatiotemporal drivers of urban water pollution:Assessment of 102 cities across the Yangtze River Basin

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Environmental Science and Ecotechnology 2024年第4期20卷 300-314页

作者： yi-Lin Zhao Han-Jun Sun Xiao-DanWang jie Ding Mei-Yun Lu ji-Wei Pang Da-Peng Zhou ming Liang Nan-Qi Ren Shan-Shan Yang State Key Laboratory of Urban Water Resource and Environment School of EnvironmentHarbin Institute of TechnologyHarbin 150090China China Energy Conservation and Environmental Protection Group Beijing 100082China China Energy Conservation and Environmental Protection Group CECEP Digital Technology Co.Ltd.Beijing 100089China China Railway Engineering Design and Consulting Group Co. Ltd.Beijing 100055China

Effective management of large basins necessitates pinpointing the spatial and temporal drivers of primary index exceedances and urban risk factors,offering crucial insights for basin ***,comprehensive examinations of multiple pollutants within the Yangtze River Basin remain *** we introduce a pollution inventory for urban clusters surrounding the Yangtze River Basin,analyzing water quality data from 102 cities during *** assessed the exceedance rates for six pivotal indicators:dissolved oxygen(DO),ammonia nitrogen(NH_(3)-N),chemical oxygen demand(COD),biochemical oxygen demand(BOD),total phosphorus(TP),and the permanganate index(COD_(Mn))for each *** random forest regression and SHapley Additive exPlanations(SHAP)analyses,we identified the spatiotemporal factors influencing these key *** results highlight agricultural activities as the primary contributors to the exceedance of all six indicators,thus pinpointing them as the leading pollution source in the ***,forest coverage,livestock farming,chemical and pharmaceutical sectors,along with meteorological elements like precipitation and temperature,significantly impacted various indicators'***,we delineate five core urban risk components through principal component analysis,which are(1)anthropogenic and industrial activities,(2)agricultural practices and forest extent,(3)climatic variables,(4)livestock rearing,and(5)principal polluting *** cities were subsequently evaluated and categorized based on these risk components,incorporating policy interventions and administrative performance within each *** comprehensive analysis advocates for a customized strategy in addressing the discerned risk factors,especially for cities presenting elevated risk levels.

关键词： Basin management Primary indices Urban risk factors Yangtze river basin Local conditions

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