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The chemical reprogramming of unipotent adult germ cells towards authentic pluripotency and de novo establishment of imprinting

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Protein & Cell 2023年第7期14卷 479-498页

作者： Yuhan Chen Jiansen Lu Yanwen Xu Yaping Huang Dazhuang Wang Peiling Liang Shaofang Ren Xuesong Hu yewen qin Wei Ke Ralf Jauch Andrew Paul Hutchins Mei Wang Fuchou Tang Xiao-Yang Zhao State Key Laboratory of Organ Failure Research Department of Developmental BiologySchool of Basic Medical SciencesSouthem Medical UniversityGuangzhou 510515China Beijing Advanced Innovation Center for Genomics School of Life SciencesPeking UniversityBeijing 100871China Biomedical Pioneering Innovation Center Ministry of Education Key Laboratory of Cell Proliferation and DifferentiationBeijing 100871China Departmentof Urology Nanfang HospitalSouthem Medical UniversityGuangzhou 510515China School of Biomedical Sciences Li Ka Shing Faculty of MedicineThe University of Hong KongHong Kong SARChina Department of Biology Southerm University of Science and TechnologyShenzhen 518055China Department of Neonatology Zhujiang HospitalSouthem Medical UniversityGuangzhou 510280China Guangdong Provincial Key Laboratory of Construction and Detection in Tissue Engineering Southem Medical UniversityGuangzhou 510515China Department of Gynecology Zhujiang HospitalSouthern Medical UniversityGuangzhou 510280China Key Laboratory of Mental Health of the Ministry of Education Guangzhou 510515China Bioland Laboratory(Guangzhou Regenerative Medicine and Health Guangdong Laboratory) Guangzhou 510005China Department of Plastic Surgery Affliated Hangzhou First People's HospitalZhejiang University School of MedicineHangzhou 310006China

Although somatic cells can be reprogrammed to pluripotent stem cells(PsCs)with pure chemicals,authentic pluripotency of chemically induced pluripotent stem celis(CipsCs)has never been achieved through tetraploid complementation *** reprogramming of spermatogonial stem cells(ssCs)was another non-transgenic way to obtain PsCs,but this process lacks mechanistic ***,we reconstructed the trajectory of mouse SsC reprogramming and developed a five-chemical combination,boosting the reprogramming effciency by nearly 80-to *** importantly,chemical induced germline-derived PsCs(5C-gPSCs),but not gpsCs and chemical induced pluripotent stem cells,had authentic pluripotency,as determined by tetraploid ***,ssCs traversed through an inverted pathway of in vivo germ ceil development,exhibiting the expression signatures and DNA methylation dynamics from spermatogonia to primordial germ cells and further to ***,ssC-specific imprinting control regions switched from biallelic methylated states to monoallelic methylated states by imprinting demethylation and then re-methylation on one of the two alleles in 5c-gPsCs,which was apparently distinct with the imprinting reprogramming in vivo as DNA methylation simultaneously occurred on both *** work sheds ight on the unique regulatory network underpinning SsC reprogramming,providing insights to understand generic mechanisms for cell-fate decision and epigenetic-relateddisorders in regenerative medicine.

关键词： reprogramming spermatogonial stem cell tetraploid complementation imprinting

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Study on the Effects of Helium-Argon Gas Mixture on the Laser Welding Performance of High Temperature Alloys

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Research and Application of Materials Science 2023年第2期5卷 21-24页

作者： Xiongzi CHEN Hesi PENG Chunchen YAO Yu DAI yewen qin Hunan Dingli Technology Co. Ltd.ChangshaHunan 410137China Changsha Tianchen Laser Technology Co. Ltd.ChangshaHunan 410100China Jiangnan Industrial Group Co. Ltd.XiangtanHunan 411207China School of Materials Science and Engineering Nanchang UniversityNanchangJiangxi330031China

In order to solve the problem of porosity in laser deep penetration welding of GH3625 high-temperature alloy plates,five different ratios of high-purity helium gas and high-purity argon gas mixed gases were compared in welding experiments after various process parameter improvements and adjustments failed to achieve Class I *** experimental results show that using high-purity helium gas or a mixture of 50%high-purity helium gas and 50%high-purity argon gas can both achieve Class I *** indicates that using high-purity helium gas or an appropriate mixed gas instead of pure argon is one of the effective ways to solve the problem of porosity in laser deep penetration welding of high-temperature *** mixture of 50%high-purity argon gas and 50%high-purity helium gas can reduce the consumption of high-purity helium gas,lower production costs,and is more suitable.

关键词： high-temperature alloy laser welding pores shielding gas welding quality

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Action of space charge on aging and breakdown of polymers

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Chinese Science Bulletin 2001年第10期46卷 796-800页

作者： LI Jixiao ZHANG yewen XIA Zhongfu qin Xiaoqiu PENG Zongren Pohl Institute of the Solid State Physics Tongji UniversityShanghai 200092China State Key Laboratory of Electrical Insulation for Power Equipment Xi’an UniversityXi’an 710049China

The study on how space charges affect aging and breakdown of polymers becomes one of the most important domains. Most of the models are based on the injected charges increasing the local field to induce the breakdown of polymers and breaking the large molecule chains. These models ignore the effects of space charge on the microstruc-ture of dielectric materials. In this review, with the calculation of the electromagnetic energy and the electromechanical energy around a trapped charge and with some new experimental results, it is proved that aging and breakdown in polymers are caused during the detrapping of the trapped charges. Aging and breakdown of the polymers are related to the release of the electromechanical energy around trapped charges.

关键词： space charge breakdown polymer.

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Screening of Host Proteins Interacting with PorcineEpidemic Diarrhea Virus (PEDV) N Protein by YeastTwo-hybrid System

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Animal Husbandry and Feed Science 2018年第4期10卷 267-271页

作者： Wang Zhongze qin Cuili Kong Ning Zuo yewen Wang Meng Zheng Hao Tong Wu Li Liwei Yu Hai Li Zhili Shan Tongling Tong Guangzhi Li Xue Henan University of Science and Technology Luoyang 471023 China Shanghai Institute of Veterinary Medicine Chinese Academy of Agricultural Sciences Shanghai 200241 China 不详

[Objective] The paper was to obtain host proteins interacting with porcine epidemic diarrhea virus （PEDV） N protein. [Method] The re-combinant vector pGBKT7-N of PEDV N gene was constructed and used as the bait plasmid to screen the proteins interacting with N protein ofPEDV from the cDNA library of porcine alveolar macrophage （PAM） by yeast two-hybrid method. [Result] There was no toxicity and self activationof bait protein in yeast hybridization system, and six proteins （FTH1, LGALS3, CORO1C, SNRPG, KRTAP5-3, ZNF598） interacting with N proteinwere indentified. It was confirmed that LGALS3 and SNRPG had specific interaction with N protein by return experiment and co-immunoprecipitation（CoIP） test. [Conclusion] The study lays a foundation for further studying the function of PEDV N protein and the pathogenic mechanism of PEDV.

关键词： Porcine epidemic diarrhea virus (PEDV) Yeast two-hybrid N protein Protein interaction

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早产儿经口置入胃管置管长度测量方法的改良及效果评价

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中华护理杂志 2022年第2期57卷 193-197页

作者：谢帅华杨芹吴旭红蒋衍李烨文周佳李微黑明燕北京国家儿童医学中心/首都医科大学附属北京儿童医院新生儿重症监护室北京市100045

目的改良早产儿经口置入胃管置管长度的测量方法,并评价其效果。方法选取北京市某三级甲等医院在新生儿中心实施经口置入胃管的306例早产儿作为研究对象。将2019年8月—2021年4月,经口置入胃管的155例早产儿作为试验组,胃管置管测量长... 详细信息

目的改良早产儿经口置入胃管置管长度的测量方法,并评价其效果。方法选取北京市某三级甲等医院在新生儿中心实施经口置入胃管的306例早产儿作为研究对象。将2019年8月—2021年4月,经口置入胃管的155例早产儿作为试验组,胃管置管测量长度的方法为从患儿鼻尖到外耳廓中点2倍的距离;将2018年3月—2019年7月经口置入胃管的151例早产儿作为对照组,胃管置管测量长度的方法为从患儿鼻尖到耳垂到剑突的距离,比较两组胃管管端到位率、胃肠减压前3 d每日的引流量、呕吐发生率及胃肠减压总时间。结果试验组经口置入胃管管端到位率为85.81%,对照组为15.23%,两组比较,差异具有统计学意义(χ^(2)=152.44,P<0.001);试验组胃肠减压前3 d引流量多于对照组,两组比较,差异具有统计学意义(t=14.38、t=15.78、t=11.06,均P<0.001);试验组胃肠减压期间呕吐发生率为9.68%,低于对照组的20.58%,两组比较,差异具有统计学意义(χ^(2)=7.05,P=0.008);试验组胃肠减压时间为(6.90±3.57)d,短于对照组的(8.27±3.88)d,两组比较,差异具有统计学意义(t=12.74,P<0.001)。结论采用改良的早产儿经口置入胃管置管长度测量方法,能有效提高胃管管端到达理想位置的精准度,避免胃管管端位置过浅或过深,并提高胃肠减压的效果。

关键词：重症监护病房早产儿胃管胃肠减压儿科护理学

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脑钠肽对AECOPD患者的生活质量评估及病情严重程度的意义

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中国社区医师 2016年第21期32卷 130-131,133页

作者：秦超刘叶文胡敏捷苏州大学附属瑞华医院 215000

目的:探讨脑钠肽对AECOPD患者生活质量的影响,以及BNP水平与病情严重程度的关系。方法:收治COPD患者40例,其中急性加重期20例为A组,稳定期20例B组。选取20例健康体检者作为C组。比较3组的血浆BNP、动脉血氧分压(Pa O2)和呼吸问卷(SGRQ)... 详细信息

目的:探讨脑钠肽对AECOPD患者生活质量的影响,以及BNP水平与病情严重程度的关系。方法:收治COPD患者40例,其中急性加重期20例为A组,稳定期20例B组。选取20例健康体检者作为C组。比较3组的血浆BNP、动脉血氧分压(Pa O2)和呼吸问卷(SGRQ)情况。结果:C组血浆BNP和Pa O2明显低于A、B组(P<0.05)。B组BNP明显低于A组(P<0.05)。A组治疗后血浆BNP值明显低于治疗前(P<0.05)。A组治疗后Pa O2明显高于治疗前(P<0.05)。COPD组SGRQ得分明显高于C组(P<0.05)。A组SGRQ得分明显高于B组(P<0.05)。A组治疗前SGRQ得分明显高于治疗后(P<0.05)。结论:COPD患者血浆BNP水平随着病情加重而升高,并且随着病情的好转BNP、Pa O2水平也逐渐好转。脑钠肽给患者生活质量带来了严重影响,对评估AECOPD患者的生活质量和病情严重程度意义重大。

关键词：脑钠肽慢性阻塞性肺疾病生活质量病情严重程度

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苦木提取物对肉鸡生长性能、屠宰性能、免疫功能及抗氧化性能的影响

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中国饲料 2023年第15期 76-80页

作者：臧青民黄华希辛桂瑜覃兰郭艺鹏卢恩科陈荣章烨雯玉林师范学院生物与制药学院广西玉林537000 广西农产资源化学与生物技术重点实验室广西玉林537000 广西高校亚热带生物资源保护与利用重点实验室广西玉林537000

为研究苦木提取物(PQE)替代抗生素对肉鸡生长性能、免疫功能、屠宰性能以及抗氧化性能的影响,试验选取300只体重相近的1日龄肉仔鸡为研究对象。对照组饲喂基础日粮,PQE1、PQE2和PQE3组分别在基础日粮中添加100、200、400 mg/kg的苦木提... 详细信息

为研究苦木提取物(PQE)替代抗生素对肉鸡生长性能、免疫功能、屠宰性能以及抗氧化性能的影响,试验选取300只体重相近的1日龄肉仔鸡为研究对象。对照组饲喂基础日粮,PQE1、PQE2和PQE3组分别在基础日粮中添加100、200、400 mg/kg的苦木提取物,抗生素组在基础日粮中添加250 mg/kg的金霉素(含量20%),试验期为42 d。结果表明:与对照组相比,PQE2组的平均日增重提高了6.3%(P 0.05)。法氏囊指数增加显著(P 0.05)。肉鸡血清抗氧化性能中,丙二醛(MDA)水平呈现下降趋势(P <0.05),其中PQE3组下降了19.15%,谷胱甘肽过氧化物酶(GSH-Px)、总抗氧化能力(T-AOC)和超氧化物歧化酶(SOD)水平也高于对照组。综上所述,在日粮中添加200 mg/kg苦木提取物对肉鸡的生长性能、免疫功能、屠宰性能和血清抗氧化性能效果最佳。

关键词：苦木提取物肉鸡生长性能免疫功能血清抗氧化屠宰性能

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