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Concentrated growth factor regulates the macrophage-mediated immune response

Regenerative Biomaterials 2021年第6期8卷 59-68页

作者： Haiyun Luo Wenjing liu Yachuan Zhou Xiao Jiang yeungyeung liu Qin Yang Longquan Shao Department of Endodontics Stomatological HospitalSouthern Medical University366 Jiangnan Avenue SouthGuangzhou 510280China Department of Prosthodontics Stomatological HospitalSouthern Medical UniversityGuangzhou 510280China State Key Laboratory of Oral Diseases&National Clinical Research Center for Oral Diseases&Department of Cariology and Endodontics West China Hospital of StomatologySichuan UniversityNO.143rd Section of Ren Min Nan Rd.Chengdu 610041China Department of Oral Medicine Stomatological HospitalSouthern Medical UniversityGuangzhou 510280China Department of Periodontics Stomatological HospitalSouthern Medical UniversityGuangzhou 510280China

Concentrated growth factor(CGF)is a promising regenerative material that serves as a scaffold and adjunct growth factor for tissue engineering.The host immune response,particularly macrophage activity,plays a critical role in injury repair and tissue regeneration.However,the biological effect of CGF on the immune response is not clear.To enrich the theoretical groundwork for clinical application,the present study examined the immunoregulatory role of CGF in macrophage functional activities in vitro.The CGF scaffold appeared as a dense fibrin network with multiple embedded leukocytes and platelets,and it was biocompatible with macrophages.Concentrated bioactive factors in the CGF extract enhanced THP-1 monocyte recruitment and promoted the maturation of suspended monocytes into adherent macrophages.CGF extract also promoted THP-1 macrophage polarization toward the M2 phenotype with upregulated CD163 expression,as detected by cell morphology and surface marker expression.A cytokine antibody array showed that CGF extract exerted a regulatory effect on macrophage functional activities by reducing secretion of the inflammatory factor interleukin-1b while inducing expression of the chemokine regulated on activation,normal T cell expressed and secreted.Mechanistically,the AKT signaling pathway was activated,and an AKT inhibitor partially suppressed the immunomodulatory effect of CGF.Our findings reveal that CGF induces a favorable immune response mediated by macrophages,which represents a promising strategy for functional tissue regeneration.

关键词： concentrated growth factor immune response macrophage signaling pathway

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