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Characterization of a novel laccase isozyme from the basidiomycete Trametes ***28-2

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Characterization of a novel laccase isozyme from the basidio...

The Eighth China-Japan-Korea Joint symposium on Enzyme Engineering

作者： Y.Z.XIAO M.Zhang H.H.GE s.X.Zhang R.Jia Y.P.Wang Laboratory of Microorganism and Gene Technology School of Life SciencesAnhui University

Laccase,a kind of polyphenol oxidase containing copper atoms,can oxidize an array of organic and inorganic substrates,including mono-,di-,and polyphenols,aminophenols,methoxyphenols as well as metal complexes:ferrocene,ferrocyanide or iodide,by concomitant four-electron reduction of oxygen to *** can be found in plants,insects and bacteria,but its major source is *** ***28-2 is a white-rot basidiomycete that selectively degrades lignin when grown on wood,and decreases the level of chemical oxygen demand in the wastewater produced in the process of straw *** studies indicate that this microorganism can be induced to secrete high levels of extracellular laccase,Laccase isozyme,laccase B,was selectively induced by 3,5-dihydroxytoluene,and purified to homogeneity by two-step *** laccase B appeared as blue with a broad peak at about 600 nm and a shoulder peak at about 330 *** ratio of absorbance at 280 nm to that at 600 nm was *** molecule of laccase B had approximately four copper *** mass of laccase B was estimated to be 74 kDa on sDs-PAGE,72 kDa by FPLC,and was determined to be 71454 Da by mass *** being treated with N-glycosidase F,laccase B lost 25%of its molecular *** isoelectric point of laccase B was *** optimal pH and temperature for oxidizing guaiacol were 4.7 and 45 ℃ *** half-life of the enzyme at 60 ℃ was 14.0 *** enzyme showed a good stability in a range of pH value 3.5 to *** K values of the enzyme toward substrates syringaldazine,guaiacol,ABTs,and DMOP were 28.0,1249.0,177.0and 109.8 μM,*** corresponding V are 504.0,1910.0,117.4 and159.0 uM ***,activity of laccase B was inhibited strongly by sodium azide and cyanide,mildly by sDs and trifluoroacetic acid,but only weakly by dimethyl sulfoxide.

关键词： 3,5-dihydroxytoluene laccase isozyme Trametes sp.ah28-2

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酒精性肝炎的治疗现状

中华肝脏病杂志 2003年第11期11卷 703-704页

作者：范建高丁晓东上海交通大学附属第一人民医院消化内科/脂肪肝诊治中心 200080

酒精性肝炎(alcoholic hepatitis,ah)目前尚无特效的治疗药物,现有多种措施主要源于动物实验研究,然而临床随机对照试验(randomised controlled trials,RCT)结果并不理想[1-3],见表1.

关键词：酒精性肝炎药物治疗糖皮质激素己酮可可碱肠内营养

重组脊髓灰质炎病毒用于治疗复发胶质母细胞瘤（英文）

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中华神经外科疾病研究杂志 2018年第4期17卷 375-375页

作者： Desjardins A Gromeier M Herndon JE 2nd Beaubier N Bolognesi DP Friedman ah Friedman Hs Mcsherry F Muscat AM Nair s Peters KB Randazzo D sampson JH Vlahovic G Harrison WT McLendon RE Ashley D Bigner DD

Background The prognosis of patients with recurrent World Health Organization(WHO)grade IV malignant glioma is dismal,and there is currently no effective *** conducted a dose-finding and toxicity study in this population of patients,evaluating convection-enhanced,intratumoral delivery of the recombinant nonpathogenic polio-rhinovirus chimera(PVsRIPO).PVsRIPO recognizes the poliovirus receptor CD155,which is widely expressed in neoplastic cells of solid tumors and in major components of the tumor *** We enrolled consecutive adult patients who had recurrent supratentorial WHO grade IV malignant glioma,confirmed on histopathological testing,with measurable disease(contrast-enhancing tumor of≥1 cm and≤5.5 cm in the greatest dimension).The study evaluated seven doses,ranging between 107 and 1010 50%tissue-culture infectious doses(TCID50),first in a dose-escalation phase and then in a dose-expansion *** From May 2012 through May 2017,a total of 61 patients were enrolled and received a dose of *** level-1(5.0×107TCID50)was identified as the phase 2 *** dose-limiting toxic effect was observed;a patient in whom dose level 5(1010TCID50)was administered had a grade 4 intracranial hemorrhage immediately after the catheter was *** mitigate locoregional inflammation of the infused tumor with prolonged glucocorticoid use,dose level 5 was deescalated to reach the phase 2 *** the dose-expansion phase,19%of the patients had a PVsRIPO-related adverse event of grade 3 or *** survival among the patients who received PVsRIPO reached a plateau of 21%(95%confidence interval,11 to 33)at 24 months that was sustained at 36 *** Intratumoral infusion of PVsRIPO in patients with recurrent WHO grade IV malignant glioma confirmed the absence of neurovirulent *** survival rate among patients who receivedPVsRIPO immunotherapy was higher at 24 and 36 months than the rate among historical controls.

关键词： WHO

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法医人类学中插入/缺失的多态性数据库

法医人类学中插入/缺失的多态性数据库

Taphonomic characterisation of tooth marks of extinct Eurasian carnivores through geometric morphometrics

第十五届国际法庭科学大会

作者： Inderjeet Kaur s.Chakrabati V.k.sarhadi A.J.s.Bhanwer J.R.s.ngh P.P.Majumder 刘雅诚 Dept.of Human Genetics G.N.D.UniversityAmritsarand B.RoyP.P.MajumderIndian Statistical Inst.CalcuttaIndia

1．在法医个体识别中多态性数据库的作用。 *** 插入/缺失在法医人类学中的应用。人类学为了解人群及其结构的特性提供了科学的基础。通过随机选择个体产生的频率分布情况通常给我们比较保守的判断。在这样一个数据库中随机偶然匹配的... 详细信息

1．在法医个体识别中多态性数据库的作用。 *** 插入/缺失在法医人类学中的应用。人类学为了解人群及其结构的特性提供了科学的基础。通过随机选择个体产生的频率分布情况通常给我们比较保守的判断。在这样一个数据库中随机偶然匹配的可能性是微不足道的。讨论应用 DNA 数据库调查频率情况,可以启发人们用 ah 插入/缺失多态性进行人群的

关键词：多态性数据库插入/缺失基因型

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science Bulletin 2022年第16期67卷 1644-1648,M0003,M0004页

作者： José Yravedra Lloyd Austin Courtenay Darío Herranz-Rodrigo Gonzalo Linares-Matás Juan JoséRodríguez-Alba Verónica Estaca-Gómez Carmen Luzón Alexia serrano-Ramos MiguelÁngel Maté-González JoséAntonio solano Diego González-Aguilera Juan Manuel Jiménez-Arenas Department of Prehistory Ancient History and ArchaeologyComplutense University of MadridMadrid 28040Spain C.A.I.Archaeometry and Archaeological Analysis Complutense UniversityMadrid 28040Spain Grupo de Investigación Ecosistemas Cuaternarios Complutense UniversityMadrid 28040Spain Grupo de Investigación Arqueología Prehistórica Complutense UniversityMadrid 28040Spain Museo Primeros Pobladores de Europa‘Josep Gibert’ Orce 18858Spain Department of Cartographic and Land Engineering Higher Polytechnic School of AvilaUniversity of SalamancaAvila 05003Spain school of Archaeology St.Hugh’s CollegeUniversity of OxfordOxford 01865United Kingdom History and Arts Doctoral Program University of GranadaGranada 18071Spain Escuela Técnica superior de Ingenieros en Topografía Geodesia y Cartografía.Universidad Politécnica de MadridMadrid 28031Spain Department of Prehistory and Archaeology University of GranadaGranada 18071Spain

Determining the cause and nature of the postmortem processes that living organisms experience is one of the main common issues faced by forensic experts,zooarchaeologists,palaeontologists,and other *** are among the most destructive agents that can interact with a corpse,since their feeding behaviour can lead to very extensive alterations,complicating the diagnostic identification of which carnivore species was responsible for the death of an individual,a livestock unit,or the formation of a fossil *** though some currently available techniques enable forensic experts to undertake a differential diagnosis of carnivore agency from corpse examination.

关键词：几何形态测量学埋藏特征有蹄类动物齿痕几何形态学机器学习分类预测多变量分析

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基于辣椒基因组测序探究辣味在种群中的进化

辣椒杂志 2014年第2期12卷 40-50页

作者： seungill Kim Minkyu Park seon-In Yeom Yong-Min Kim Je Min Lee Hyun-ah Lee Eunyoung seo Jaeyoung Choi Kyeongchae Cheong Ki-Tae Kim Kyongyong Jung Gir-Won Lee s.ng-Keun Oh Chungyun Bae saet-Byul Kim Hye-Young Lee shin-Young Kim Myung-shin Kim Byoung-Cheorl Kang yeong Deuk Jo Hee-Bum Yang Hee-Jin Jeong Won-Hee Kang Jin-Kyung Kwon Chanseok shin Jae Yun Lim June Hyun Park Jin Hoe Huh June-sik Kim Byung-Dong Kim Oded Cohen Ilan Paran Mi Chung suh saet Buyl Lee yeon-Ki Kim Younhee shin seung-Jae Noh Junhyung Park Young sam seo suk-Yoon Kwon Hyun A Kim Jeong Mee Park Hyun-Jin Kim s.ng-Bong Choi Paul W Bosland Gregory Reeves s.ng-Hwan Jo Bong-Woo Lee Hyung-Taeg Cho Hee-seung Choi Min-soo Lee Yeisoo Yu Yang Do Choi Beom-seok Park Allen van Deynze Hamid Ashrafi Theresa Hill Woo Taek Kim Hyun-sook Pai Hee Kyung ahn Inhwa Yeam James J Giovannoni Jocelyn K C Rose Iben sφrensen s.ng-Jik Lee Ryan W Kim Ik-Young Choi Beom-soon Choi Jong-s.ng Lim Yong-Hwan Lee Doil Choi 刘峰张西露 Department of Plant science seoul National University Plant Genomics and Breeding Institute Seoul National University Department of Agricultural Biotechnology Seoul National University Department of Bioinformatics and Life science Soongsil University Vegetable Breeding Research Center Seoul National University Agricultural Research Organization Institute of Plant Science Volcani Center Bet DaganIsrael. Agricultural Research Organization Institute of Plant Science Volcani Center Bet DaganIsrael Department of Bioenergy science and Technology Chonnam National University Genomics Genetics Institute GreenGene BioTech Inc. Codes Division Insilicogen Inc. Gins.ng Resources Research Laboratory Korea Ginseng Corporation Korea Research Institute of Bioscience and Biotechnology Division of Bioscience and Bioinformatics Myongji University Department of Plant and Environmental sciences New Mexico State University Chile Pepper Institute New Mexico State University seeders Inc. Department of Biological sciences Seoul National University Arizona Genomics Institute University of Arizona Agricultural Genome Center National Academy of Agricultural Science Rural Development Administration seed Biotechnology Center University of California Davis Davis California USA Department of systems Biology Yonsei University Department of Horticulture and Breeding Andong National University Us Department of Agriculture-Agricultural Research service Robert W. Holley Center Ithaca New York USA Boyce Thompson Institute for Plant Research Cornell University Ithaca New York USA Department of Plant Biology Cornell University Ithaca New York USA Biotechnology Institute Nongwoo Bio Yeoju Korea Genome Center University of California Davis Davis California USA National Instrumentation Center for Environmental Management Seoul National University Seoul Korea 湖南省蔬菜研究所

辣椒(Capsicum annuum)是美洲最古老的驯化作物之一,在全世界被广泛种植。本研究对CM334(墨西哥地方品种)进行了全基因组测序和组装(覆盖度为186.6×),同时还对另外两个栽培种和一个野生种分别进行了重测序和从头(de novo)测序。结果表... 详细信息

辣椒(Capsicum annuum)是美洲最古老的驯化作物之一,在全世界被广泛种植。本研究对CM334(墨西哥地方品种)进行了全基因组测序和组装(覆盖度为186.6×),同时还对另外两个栽培种和一个野生种分别进行了重测序和从头(de novo)测序。结果表明辣椒基因组大小约为番茄的4倍,辣椒基因组上存在大量gypsy转座子和花椰菜病毒家族元件的积累。结合转录组综合分析认为,辣椒素合成酶基因表达模式的改变及新功能化是导致辣椒素生物合成的原因,研究还发现辣椒与番茄在乙烯合成及果实成熟调控方面存在明显不同的分子模式。辣椒参考基因组的发布为辣椒营养品质和药用价值的改善提供了重要平台。

关键词：基因组测序辣椒素参考基因 Capsicum 转座子辣椒果实分子模式生物合成基因表达模式基因组大小

活动性葡萄膜炎患者房水中的趋化因子和明胶酶

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世界核心医学期刊文摘（眼科学分册） 2005年第3期1卷 34-35页

作者： Abu El Asrar A.M. struyf s. Descamps F.J. 秦雪娇 Department of Ophthalmology College of Medicine Kin g Saud University Riyadh Saudi Arabia Dr

Purpose To investigate the involvement of the chemokines CXCL10/ IP-10, CXCL1 1/I-TAC, CXCL8/1L-8, CXCL6/GCP-2, CCL3/MIP-1α, and CCL18/PARC, and gelatina ses A and B in uveitis. Design Prospective, experimental, case control study. M ethods Aqueous humor samples from 30 patients with active uveitis, and 14 contro l patients and paired serum samples were assayed for chemokines with specific en zyme linked immunosorbent assays (ELIsAs) and for gelatinase levels by quantita tive zymography. Results In control ah, none of the chemokines was detected. Gel atinase A was detected in all samples, and gelatinase B was detected in only one sample. In patients with uveitis, IP-10 was detected in all ah samples, wherea s I-TAC, IL-8, GCP-2, MIP-1α, and PARC were detected in three, 16, six, two , and 12 samples, respectively. IP-10 levels were significantly higher in ah sa mples than those of serum (P=.006). Gelatinase A was detected in 29 ah samples a nd gelatinase B was detected in 26 samples. Gelatinase A levels were significant ly higher in ah samples from patients than those of controls (Psamples, gelatinase B was detected in complex with lipocalin (ngAL). Disease a ctivity correlated significantly with the levels of IP 10 (r=.627; Pse A (r=.508 ; P=.002), gelatinase B (r=.685 ; PngAL gelat inase B complex (r=.595 ; Psions These data suggest a pathogenic role of the T lymphocyte chemoattractant IP 10 and gelatinases in the recruitm ent and activity of T cells into the eye in patients with uveitis and in the pat hogenesis of uveitis.

关键词：葡萄膜炎明胶酶趋化因子脂质运载蛋白特异性酶血清样本定量性病例对照研究复合体趋化作用

高稳定人胰岛素的晶体学研究:Ⅲ,A21-Asp突变体的结晶和初步晶体学分析

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生物物理学报 1992年第1期8卷 76-80页

作者：金雷黄伟军伍伯牧王大成 s.Havelund 中国科学院生物物理研究所北京100101 NOVO Research Institute DK-2880 Bagsvaerd Denmark

研究一系列有明确意义改性胰岛素的结构将有益于新型胰岛素分子的设计。经蛋白质工程制备的AspA21—人胰岛素(ADHI)基本保持了生物活力,而在酸性溶夜(pH3—4)中的稳定性比天然胰岛素在中性溶液中高5—10倍。本文报道ADHI的结晶、初步晶... 详细信息

研究一系列有明确意义改性胰岛素的结构将有益于新型胰岛素分子的设计。经蛋白质工程制备的AspA21—人胰岛素(ADHI)基本保持了生物活力,而在酸性溶夜(pH3—4)中的稳定性比天然胰岛素在中性溶液中高5—10倍。本文报道ADHI的结晶、初步晶体学研究及中等分辨率衍射数据的收集。ADHI晶体属R3空间群:a_H=b_H=82.72埃,c_H=34.02埃。

关键词：人胰岛素晶体学 A21Asp突变体

使Arealometer自动化——检验Kozeny公式

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纤维标准与检验 1993年第2期 28-30,18页

作者： K.E.Duckett J.Cain R.s.Krowicki D.P.Thibodeaux 敬人美国田纳西大学人类生态学院美国农业部农业研究局南方地区研究中心

在一种实验用计算机化的系统上,对Arealometer度量的11个具有广泛细度和成熟度范围的棉样,进行了气流习性研究。对范围为0.6～0.9的孔隙度按Kozeny公式求得比表面积。观测到用孔隙度得到的纤维细度变异性是原先Arealometer所预料不到的... 详细信息

在一种实验用计算机化的系统上,对Arealometer度量的11个具有广泛细度和成熟度范围的棉样,进行了气流习性研究。对范围为0.6～0.9的孔隙度按Kozeny公式求得比表面积。观测到用孔隙度得到的纤维细度变异性是原先Arealometer所预料不到的。然而,分别在低和高试样压缩下的比表面积A与ah却和Arealometer结果非常相关。当新型的自动系统用固定孔隙度而不用类似装在Arealometer中的固定多孔塞气阻时,所得到的值具有最佳相关性。

关键词：棉花细度自动化系统 Kozeny公式