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3D tumor model biofabrication

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Bio-Design and Manufacturing 2021年第3期4卷 526-540页

作者： Ming Li Xueer Song sha Jin Kaiming ye Department of Biomedical Engineering Center of Biomanufacturing for Regenerative MedicineBinghamton UniversityState University of New York(SUNY)PO Box 6000BinghamtonNY 13902USA

Animal models have been extensively used in cancer pathology studies and drug *** models,however,fail to reflect the complex human tumor microenvironment and do not allow for high-throughput drug screening in more human-like physiological ***-dimensional(3D)cancer models present an alternative to automated high-throughput cancer drug discovery and *** this review,we highlight recent technology innovations in building 3D tumor models that simulate the complex human tumor microenvironment and responses of patients to *** discussed various biofabrication technologies,including 3D bioprinting techniques developed for characterizing tumor progression,metastasis,and response to treatment.

关键词： Tumor models Biofabrication Tumor spheroids Microfluidic devices 3D bioprinting

Carbon dioxide adsorption behaviors of aluminum-pillared montmorillonite-supported alkaline earth metals

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Journal of Environmental Sciences 2020年第12期32卷 109-117页

作者： Kai Wu Qing ye Runping Wu sha Chen Hongxing Dai Key Laboratory of Beijing on Regional Air Pollution Control Department of Environmental ScienceCollege of Environmental and Energy EngineeringBeijing University of TechnologyBeijing 100124China Beijing Key Laboratory for Green Catalysis and Separation Key Laboratory of Beijing on Regional Air Pollution ControlKey Laboratory of Advanced Functional MaterialsEducation Ministry of Chinaand Laboratory of Catalysis Chemistry and NanoscienceDepartment of Chemistry and Chemical EngineeringCollege of Environmental and Energy EngineeringBeijing University of TechnologyBeijing 100124China

The Al-pillared montmorillonite-supported alkaline earth metal 5M/Al-PILC(PILC = pillared clay, M = Mg, Ca, Sr, and Ba) and x Mg/Al-PILC( x = 1, 3, 5, and 7 wt.%) samples were prepared using an impregnation method. Physical properties of the materials were determined by means of X-ray diffraction(XRD) and N2 adsorption-desorption, and their CO2 adsorption behaviors were investigated using the thermogravimetric analyzer(TG), CO2 temperatureprogrammed desorption(CO2-TPD), and in situ diffuse reflectance infrared transform spectroscopy(in situ-DRIFTS) techniques. It is shown that 5 Mg/Al-PILC possessed the highest CO2 adsorption capacity(2.559 mmol/g). The characterization results indicate that Alpillaring increased the specific surface area of montmorillonite, which was beneficial for the adsorption of CO2. The CO2 adsorption process on the sample was mainly chemical adsorption, and alkalinity was the main factor influencing its adsorption capacity. The alkalinity of the sample was enhanced by loading an appropriate amount of alkaline earth metal, and the adsorbed CO2 was present in the form of bicarbonate and carbonate. In addition, the 5Mg/Al-PILC sample exhibited an excellent regeneration efficiency. We believe that the outcome of this research would provide a good option for developing highly effective CO2 adsorption materials.

关键词： Montmorillonite Al-pillaring Adsorbent CO2 adsorption Alkaline earth metal

Influence of Kinematic Analysis Parameters of Drag Anchor Trajectory Prediction Using Yield Envelope Method

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China Ocean Engineering 2020年第2期34卷 257-266页

作者： WU Xiao-ni WANG Teng LIAO Qian LI ye State Key Laboratory of Ocean Engineering School of Naval ArchitectureOcean and Civil EngineeringShanghai Jiao Tong UniversityShanghai 200240China Department of Naval Architecture and Offshore Engineering China University of Petroleum(East China)Qingdao 266580China

Drag anchor is widely applied in offshore engineering for offshore mooring *** prediction of the invisible trajectory during its drag-in installation is challenging for anchor design in determining the anchor final position for ensuring sufficient holding *** yield envelope method based on deep anchor failure for kinematic analysis was proposed as a promising trajectory prediction method for drag ***,there is a lack of analysis on the effects of the parameters applied in the kinematic *** current work studies the effects of the yield envelope parameters,anchor line bearing capacity factor and the anchor/soil interface *** is found that the accuracy of the yield envelope parameters has large impact on the prediction results based on deep yield *** of cases with smooth fluke predict deeper embedment depth than that from analyses of cases with rough *** decrease of the capacity factor results in the increase of the anchor embedment depth,the anchor line load,the anchor chain angle and the stable value of the normalized horizontal load component for the same drag length,while the stable value of the normalized vertical load component decreases when the capacity factor *** illustrates the importance in applying reasonable parameters and improving the method for more reliable prediction of the anchor trajectory.

关键词： trajectory prediction kinematic analysis drag anchor yield envelope

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三种芸香糖苷酶的底物特异性和酶学特征

食品工业科技 2025年第2期46卷 25-34页

作者：肖枫伟孙江叶家影李利君倪辉集美大学海洋食品与生物工程学院福建省食品微生物与酶工程重点实验室厦门市食品生物工程技术研究中心厦门海洋职业技术学院海洋生物学院

芸香糖苷类黄酮化合物的水解产物槲皮素、柚皮素、橙皮素具有丰富的生物活性和应用前景。为了探讨不同芸香糖苷酶水解制备槲皮素、柚皮素和橙皮素的特性，选择了Aspergillus niger CBS 513.88的芸香糖苷酶AnRut,Acremonium sp. DSM 2469... 详细信息

芸香糖苷类黄酮化合物的水解产物槲皮素、柚皮素、橙皮素具有丰富的生物活性和应用前景。为了探讨不同芸香糖苷酶水解制备槲皮素、柚皮素和橙皮素的特性，选择了Aspergillus niger CBS 513.88的芸香糖苷酶AnRut,Acremonium sp. DSM 24697的αRβD Ⅰ和αRβD Ⅱ进行底物特异性和酶学性质的比较研究。结果表明，三种芸香糖苷酶均只能水解α-1,6连接的芸香糖苷类黄酮化合物，对α-1,2连接的新橙皮糖苷类黄酮化合物无作用。AnRut偏好水解3-O连接的芦丁，αRβD Ⅰ偏好水解7-O连接的芸香柚皮苷和橙皮苷，αRβD Ⅱ对两种类型底物都具有良好的水解活性。分子对接结果表明三种酶与芦丁、芸香柚皮苷和橙皮苷的结合模式不同，三种酶与底物的苷元结构的相互作用差异影响了底物特异性。AnRut最适温度50℃，最适pH4.0,10 mmol/L的β-ME和DTT对AnRut酶活有明显促进作用，相对酶活是未处理酶的223%和242%。αRβD Ⅰ最适温度70℃，最适pH4.0，在酸性条件下对芸香柚皮苷和橙皮苷具有良好的水解活性。αRβD Ⅱ最适温度40℃，最适pH6.0，较适用于中性条件下对三种底物的水解。本研究为应用不同芸香糖苷酶制备槲皮素、柚皮素、橙皮素以及芸香糖苷酶构效关系的后续研究提供了实验和理论依据。

关键词：芸香糖苷酶芸香糖苷类黄酮底物特异性酶学特征分子对接

Scattering of Water Waves by Dual Symmetric Inclined Floating Porous Barriers Using the DBEM

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China Ocean Engineering 2024年第1期38卷 156-168页

作者： WANG Li-xian DENG Yan-wen ye Yang-sha DENG Zheng-zhi Key Laboratory of High Performance Ship Technology(Wuhan University of Technology) Ministry of EducationWuhan 430063China School of Naval Architecture Ocean and Energy Power EngineeringWuhan University of TechnologyWuhan 430063China Key Laboratory of Water-Sediment Sciences and Water Disaster Prevention of Hunan Province Changsha 410114China Institute of Port Coastal and Offshore EngineeringZhejiang UniversityZhoushan 316021China

The scattering of normally incident water waves by two surface-piercing inclined perforated barriers in water with a uniform finite depth is investigated within the framework of linear water wave *** that thin barriers are zero-thickness,a novel numerical method involving the the coupling of the dual boundary element method(DBEM)with damping layers is *** order to effectively damp out the reflected waves,two damping layers,instead of pseudoboundaries are implemented near the two side boundaries of the computational ***,the modified linearized free surface boundary conditions are formulated and used for solving both the ordinary boundary integral equation as well as the hypersingular boundary integral equation for degenerate *** newly developed numerical method is validated against analytical methods using the matched eigenfunction expansion method for the special case of two vertical barriers or the inclined angle to the vertical being *** influence of the length of the two damping layers has been ***,these findings are also validated against previous results for several *** validation,the numerical results for the reflection coefficient,transmission coefficient and dissipation coefficient are obtained by varying the inclination angle and porosity-effect *** effects of both the inclination angle and the porosity on the amplitudes of wave forces acting on both the front and rear barriers are also *** is found that the effect of the inclination angle mainly shifts the location of the extremal values of the reflection and the transmission ***,a moderate value of the porosity-parameter is quite effective at dissipating wave energy and mitigating the wave loads on dual barriers.

关键词： dual boundary element method inclined perforated floating breakwater reflection coefficient transmission coefficient damping layer

Multi-enzyme cascade for sustainable synthesis of L-threo-phenylserine by modulating aldehydes inhibition and kinetic/thermodynamic controls

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Systems Microbiology and Biomanufacturing 2022年第4期2卷 705-715页

作者： Yuansong Xiu Guochao Xu ye ni Key Laboratory of Industrial Biotechnology Ministry of EducationSchool of BiotechnologyJiangnan UniversityWuxi 214122JiangsuChina

l-Threonine transaldolase could catalyze the transaldolation of l-threonine and aldehyde to generateβ-hydroxy-α-amino acids with high diastereoselectivity.A novel l-threonine transaldolase(PmLTTA)was identified from Pseudomonas *** genome *** exhibited high activity in the synthesis of l-threo-phenylserine from l-threonine and benzaldehyde,with specific activity of 5.48 U ***,the application of PmLTTA was impeded by the low conversion ratio and variable diastereoselectivity,which were caused by the toxicity of aldehydes and kinetic/thermodynamic controls in the transaldolation *** solve these issues,alcohol dehydrogenase was used to remove the by-product acetaldehyde,and then carboxylic acid reductase was introduced to alleviate the inhibition of benzaldehyde and toxicity of ***,a multi-enzyme cascade reaction,comprising of PmLTTA,carboxylic acid reductase,alcohol dehydrogenase and glucose dehydrogenase,was constructed to prepare l-threo-phenylserine from cheap benzoic acid,in which alleviated inhibition of aldehydes and desirable diastereoselectivity were *** the optimized conditions,the conversion ratio of 57.1%and de value of 95.3%were *** study provides an efficient and green approach for the synthesis of chiral l-threo-phenylserine from industrial byproduct,and provides guidance for the development of cascade reactions influenced by the toxic intermediates and complicated kinetic/thermodynamic controls.

关键词： L-Thronine transaldolase l-Threo-phenylserine Reaction optimization Cascade reaction Diastereoselectivity

Quantum State Transfer for Two Atoms with a Single Resonant Interaction

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Communications in Theoretical Physics 2007年第5X期48卷 831-833页

作者： WU Tao ni Zhi-Xiang ye Liu Department of Physics Fuyang Teachers College Fuyang 236041 China School of Physics & Material Science Anhui University Hefei 230039 China

We present a scheme for transferring an unknown atomic entangled state with a single resonant interaction. This scheme only requires a single resonant interaction of two atoms with a cavity mode and does not use the cavity mode as the memory. Thus the scheme is very simple and the interaction time is very short, which is important, in view of decoherence. Quantum state can be directly transferred from two atoms to another two at, oms with a successful probability of 100 percent.

关键词： transfer cavity QED resonant interaction quantum entanglement

Novel Stereoselective Carbonyl Reductase from Kluyveromyces marxianus for Chiral Alcohols Synthesis

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Chemical Research in Chinese Universities 2013年第6期29卷 1140-1148页

作者： LI Hai-dong SUN Zhi-hao ni ye Key Laboratory of Industrial Biotechnology Ministry of Education School of BiotechnologyJiangnan University Wuxi 214122 P.R.China

A novel nicotinamide adenine dinucleotide phosphate（NADPH）-dependent carbonyl reductase from Kluyverornyces marxianus（KmCR） was identified, which can convert various prochiral ketone esters and ketone substrates to their corresponding chiral alcohols. KmCR was over-expressed in E. coli BL21（DE3）, purified to homogeneity, and characterized. The purified enzyme exhibits the highest activity at 40℃ and pH=6.0. Based on the gel filtration and sodium dodecyl sulfate-polyacrylamide gel eiectrophoresis（SDS-PAGE） analysis, the monomeric protein was determined to have a molecular weight of approximate 39000. Vmax and Km of KmCR are 4.28 μ***^-1·mg^-1 and 0.41 mmol/L for ketone ester substrate ethyl 2-oxo-4-phenylbutyrate（OPBE）, 3.09μ***^-1·mg^-1 and 1.21 mmol/L for cofactor NADPH, respectively. Cofactor recycle was achieved by co-expression of KmCR and glucose dehydrogenase（GDH） in E. coli. Recombinant E. coli harboring KmCR and GDH showed moderate asymmetric reduction activity towards various α- and β-ketoesters, diaryl ketone substrates. In an aqueous/butyl acetate biphasic system, the whole-cell biocatalyst was used to prepare ethyl （R）-2-hydroxy-4- phenylbutanoate[（R）-HPBE] in an e.e. of 99.5% with a space-time yield of 433.6 g.L-1.d-1 and a yield of 80.3% at 270 g/L OPBE.

关键词： Carbonyl reductase Asymmetric reduction Chiral alcohol co-Expression Kluyveromyces marxianus

Transferring of a Two-Mode Entangled State Between Two Cavities via Cavity QED

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Communications in Theoretical Physics 2007年第3期47卷 451-453页

作者： WU Tao ni Zhi-Xiang ye Liu School of Physics ＆ Material Science Anhui University Hefei 230039 China Department of Physics Fuyang Teachers College Fuyang 236032 China

We propose a scheme for transferring of a two-mode entanglement of zero- or one-photon entangled states between two cavities via atom-cavity field resonant interaction. In our proposal, in order to transfer the entangled state, we only need two identical two-level atoms and a two-mode cavity for receiving the teleported state. This scheme does not require Bell-state measurement and performing any transformations to reconstruct the initial state. And the transfer can occur with 100% success probability in a simple manner. And a network for transferring of a two-mode entangled state between cavities is suggested. This scheme can also be extended to transfer N-mode entangled state of cavity.

关键词： transfer cavity QED quantum entanglement resonant interaction