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Antibody-platinum(IV)prodrugs conjugates for targeted treatment of cutaneous squamous cell carcinoma

Journal of Pharmaceutical Analysis 2024年第3期14卷 389-400页

作者： Xiangye Yin Yingjie Zhuang Haiqin Song Yujian Xu Fan zhang Jianxin Cui Lei Zhao Yingjie Yu Qixu zhang Jun ye Youbai Chen Yan Han Department of Plastic and Reconstructive Surgery The First Medical CenterChinese PLA General HospitalBeijing100853China Department of General Surgery Ruijin HospitalShanghai Jiao Tong University School of MedicineShanghai200023China State Key Laboratory of Organic-Inorganic Composites Beijing Laboratory of Biomedical MaterialsBeijing University of Chemical TechnologyBeijing100029China Department of Plastic Surgery University of Texas MD Anderson Cancer CenterHoustonTX77030USA State Key Laboratory of Bioactive Substance and Function of Natural Medicines Institute of Materia MedicaChinese Academy of Medical Sciences&Peking Union Medical CollegeBeijing100050China

Antibody-drug conjugates(ADCs)are a new type of targeting antibodies that conjugate with highly toxic anticancer drugs via chemical linkers to exert high specificity and efficient killing of tumor cells,thereby attracting considerable attention in precise oncology ***(Cet)is a typical antibody that offers the benefits of good targeting and safety for individuals with advanced and inoperable cutaneous squamous cell carcinoma(cSCC);however,its anti-tumor activity is limited to a single ***(CisPt)shows good curative effects;however,its adverse effects and non-tumor-targeting ability are major *** this study,we designed and developed a new ADC based on a new cytotoxic platinum(IV)prodrug(C8Pt(IV))and *** so-called antibody-platinum(IV)prodrugs conjugates,named Cet-C8Pt(IV),showed excellent tumor targeting in ***,it accurately delivered C8Pt(IV)into tumor cells to exert the combined anti-tumor effect of Cet and ***,metabolomic analysis showed that Cet-C8Pt(IV)promoted cellular apoptosis and increased DNA damage in cSCC cells by affecting the vitamin B6 metabolic pathway in tumor cells,thereby further enhancing the tumor-killing ability and providing a new strategy for clinical cancer treatment using antibody-platinum(IV)prodrugs conjugates.

关键词： Antibody drug conjugate Cutaneous squamous cell carcinoma DNA damage Platinum drug Targeted therapy

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Supercoiled DNA Minicircles under Double-strand Breaks

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Chinese Journal of Polymer Science 2024年第9期42卷 1353-1359,I0007页

作者： ye-Peng Qiao Chun-Lai Ren National Laboratory of Solid State Microstructures and Department of Physics Collaborative Innovation Center of Advanced MicrostructuresNanjing UniversityNanjing210093China

Understanding how supercoiled DNA releases intramolecular stress is essential for its functional ***,the molecular mechanism underlying the relaxation process remains insufficiently *** we employed md simulations based on the oxDNA2 model to investigate the relaxation process of a 336-base pair supercoiled minicircular DNA under double-strand breaks with two fixed *** simulations show that the conformational changes in the DNA occur continuously,with intramolecular stress release happening abruptly only when the DNA chain traverses the breakage *** relaxation process is influenced not only by the separation distance between the fixed ends but also their ***,we observe an inhibitory effect on the relaxation characterized by small angles,where short terminal loops impede DNA conformational adjustments,preserving the supercoiled *** findings elucidate the intricate interplay between DNA conformational change,DNA motion and intramolecular stress release,shedding light on the mechanisms governing the relaxation of supercoiled DNA at the molecular level.

关键词： Supercoiled DNA DNA minicircle DNA relaxation oxDNA model md simulation

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细粒度情感和情绪分析中损失函数的设计与优化

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中文信息学报 2024年第1期38卷 124-134页

作者：叶施仁丁力 Ali md Rinku 常州大学计算机与人工智能学院江苏常州213164

在细粒度情感分析和情绪分析数据集中,标签之间的相关性和标签分布的不均匀性非常突出。类别标签分布不均匀,标签之间存在相关性容易影响学习模型的性能。针对这一问题,该文受计算机视觉领域中的Circle loss方法的启发,将梯度衰减、成... 详细信息

在细粒度情感分析和情绪分析数据集中,标签之间的相关性和标签分布的不均匀性非常突出。类别标签分布不均匀,标签之间存在相关性容易影响学习模型的性能。针对这一问题,该文受计算机视觉领域中的Circle loss方法的启发,将梯度衰减、成对优化、添加余量引入损失函数来优化深度学习模型的性能。该方法可以很好地与预训练模型相结合,不需要修改骨干网络。与当前最新的经典方法相比,该方法在SemEval18数据集上Jaccard系数、micro-F1、macro-F1分别提升了1.9%、2%、1.9%;在GoeEmotions数据集上Jaccard系数、micro-F1、macro-F1分别提升了2.6%、1.9%、3.6%。实验表明,该文提出的损失函数对情感分析和情绪分析问题具有显著的提升作用。

关键词：情感分析情绪分析成对优化损失函数

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Exploring pore-scale production characteristics of oil shale after CO_(2) huff‘n’puff in fractured shale with varied permeability

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International Journal of Coal Science & Technology 2024年第1期11卷 194-203页

作者： Tianhan Xu Jian Wang Yuhao Lu Danling Wang Li Yu ye Tian State Key Laboratory of Oil and Gas Reservoir Geology and Exploitation Southwest Petroleum UniversityChengdu610500SichuanChina Institute for Carbon Neutrality Southwest Petroleum UniversityChengdu610500SichuanChina Research Institute of Shenzhen Branch of CNOOC(China)Co. LtdShenzhen518000GuangdongChina PetroChina Huabei Oilfield Company Cangzhou062552HebeiChina

Recent studies have indicated that the injection of carbon dioxide(CO_(2))can lead to increased oil recovery in fractured shale reservoirs following natural *** advancements in understanding mass exchange processes in subsurface formations,there remains a knowledge gap concerning the disparities in these processes between the matrix and fractures at the pore scale in formations with varying *** study aims to experimentally investigate the CO_(2) diffusion behaviors and in situ oil recovery through a CO_(2) huff‘n’puff process in the Jimsar shale oil *** achieve this,we designed three matrix-fracture models with different permeabilities(0.074 md,0.170 md,and 0.466 md)and experimented at 30 MPa and 91℃.The oil concentration in both the matrix and fracture was monitored using a low-field nuclear magnetic resonance(LF-NMR)technique to quantify in situ oil recovery and elucidate mass-exchange *** results showed that after three cycles of CO_(2) huff‘n’puff,the total recovery degree increased from 30.28%to 34.95%as the matrix permeability of the core samples increased from 0.074 to 0.466 md,indicating a positive correlation between CO_(2) extraction efficiency and matrix *** similar fracture conditions,the increase in matrix permeability further promoted CO_(2) extraction efficiency during CO_(2) huff‘n’***,the increase in matrix permeability of the core had the greatest effect on the extraction of the first-cycle injection in large pores,which increased from 16.42%to 36.64%.The findings from our research provide valuable insights into the CO_(2) huff‘n’puff effects in different pore sizes following fracturing under varying permeability conditions,shedding light on the mechanisms of CO_(2)-enhanced oil recovery in fractured shale oil reservoirs.

关键词： Shale reservoir Hydraulic fracturing CO_(2)huff‘n’puff NMR Production characteristics

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Functional characterization,structural basis,and protein engineering of a rare flavonoid 2′-O-glycosyltransferase from Scutellaria baicalensis

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Acta Pharmaceutica Sinica B 2024年第8期14卷 3746-3759页

作者： Zilong Wang Xueqing Du Guo ye Haotian Wang Yizhan Liu Chenrui Liu Fudong Li HansÅgren Yang Zhou Junhao Li Chao He De-An Guo Min ye State Key Laboratory of Natural and Biomimetic Drugs School of Pharmaceutical SciencesPeking UniversityBeijing 100191China Anhui Key Laboratory of Modern Biomanufacturing and School of Life Sciences Anhui UniversityHefei 230601China National Science Center for Physical Sciences at Microscale Division of Molecular&Cell Biophysics and School of Life Sciences University of Science and Technology of ChinaHefei 230026China Department of Physics and Astronomy Uppsala UniversityUppsala SE-75120Sweden School of Pharmacy Jinan UniversityGuangzhou 510632China Shanghai Research Center for Modernization of Traditional Chinese Medicine Shanghai Institute of Materia MedicaChinese Academy of SciencesShanghai 201203China

Glycosylation is an important post-modification reaction in plant secondary metabolism,and contributes to structural diversity of bioactive natural *** plants,glycosylation is usually catalyzed by *** 2′-O-glycosides are rare ***,no UGTs have been reported,thus far,to specifically catalyze 2′-O-glycosylation of *** this work,UGT71AP2 was identified from the medicinal plant Scutellaria baicalensis as the first flavonoid 2′-*** could preferentially transfer a glycosyl moiety to 2′-hydroxy of at least nine flavonoids to yield six new *** of the 2′-O-glycosides showed noticeable inhibitory activities against cyclooxygenase *** crystal structure of UGT71AP2(2.15Å)was solved,and mechanisms of its regio-selectivity was interpreted by pKa calculations,molecular docking,md simulation,MM/GBSA binding free energy,QM/MM,and hydrogen‒deuterium exchange mass spectrometry *** structure-guided rational design,we obtained the L138T/V179D/M180T mutant with remarkably enhanced regio-selectivity(the ratio of 7-O-glycosylation byproducts decreased from 48%to 4%)and catalytic efficiency of 2′-O-glycosylation(kcat/Km,0.23 L/(s·μmol),12-fold higher than the native).Moreover,UGT71AP2 also possesses moderate UDP-dependent de-glycosylation activity,and is a dual function *** work provides an efficient biocatalyst and sets a good example for protein engineering to optimize enzyme catalytic features through rational design.

关键词： Glycosyltransferase Regio-selectivity Crystal structure De-glycosylation Catalytic mechanisms

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鞘内注射吗啡预处理激活大鼠δ、κ、μ受体致心肌保护

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麻醉与镇痛 2009年第6期5卷 22-28页

作者： Rui Li Gordon T. C. Wong Tak Ming Wong ye zhang, md Zhengyuan Xia Michael G. Irwin 潘鹏(译) 李文志(校) Department of Anesthesiology University of Hong Kong Hong Kong Department of Anesthesiology Anhui Medical University China Department of Physiology University of Hong Kong Hong Kong 不详

背景鞘内注射小剂量吗啡可产生与静脉注射吗啡相似的心肌保护作用，但鞘内注射吗啡预处理（IT—MPC）与缺血预处理（IPC）作用强度的差别还未知，IT-MPC是否由阿片类受体所介导也未阐明。因此，本实验比较IT—MPC与IPC作用强度差别并探... 详细信息

背景鞘内注射小剂量吗啡可产生与静脉注射吗啡相似的心肌保护作用，但鞘内注射吗啡预处理（IT—MPC）与缺血预处理（IPC）作用强度的差别还未知，IT-MPC是否由阿片类受体所介导也未阐明。因此，本实验比较IT—MPC与IPC作用强度差别并探讨阿片类受体的作用。方法80只SD大鼠麻醉后开胸，成功置入鞘内导管后随机分为13组（n=6—7）。IPC组接受3次5分钟缺血-5分钟再灌注的心肌IPC（阻闭左冠状动脉），随之给予30分钟缺血-2小时再灌注诱导心肌缺血再灌注（IR）损伤。IT-MPC组按剂量不同分为4个亚组（分别是0．03、0．3、3及30μg／kg），以3次5分钟注射-5分钟间停的模式平均注入鞘内，随之诱导IR损伤。静脉注射吗啡预处理组（IV-MPC）组经静脉给予吗啡300μg／kg，对照组鞘内给予10μl生理盐水。受体阻滞剂组选择在IT—MPC（3μg/kg）前分别给予选择性δ、κ、μ受体阻滞剂NTD、nor-BNI及CTOP，以评价阿片类受体亚型的作用。通过2，3，5-tripheny ltetrazolium染色，计算心肌梗死面积（IS），即梗死心肌占缺血危险区（AAR）的百分比。结果与对照组相比，鞘内注射0．3～30μg／kg吗啡降低心肌IS，3组IS／AAR分别为33％±10％（0．3μg／kg）、29％±10％（3μg／kg）、29％±16％（30μg/kg），对照组为53％±8％（P〈0．01）。IT-MPC降低心肌IS／AAR程度与IV-MPC（33％±6％，P=0．84）及IPC（22％±4％，P=0．41）相近。注射选择性受体阻滞剂后，由IT-MPC（3μg/kg）产生的心肌预处理效果降低（NTD＋IT-MPC，50％±9％：nor-BNI＋IT-MPc，43％±6％；CTOP＋IT—MPC，53％±9％；与对照组相比，P=0．14）。结论IT—MPC产生与IPC及IV—MPC相近的心肌保护作用，其机制涉及到δ、κ、μ受体。

关键词：心肌保护作用小剂量吗啡缺血预处理鞘内注射 SD大鼠 μ受体心肌缺血再灌注阿片类受体

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Dynamics design of a non-natural transcription factor responding to androst-4-ene-3,17-dione

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Synthetic and Systems Biotechnology 2024年第3期9卷 436-444页

作者： Ming Zhao Mengkai Hu Rumeng Han Chao ye Xiangfei Li Tianwen Wang Yan Liu Zhenglian Xue Kun Liu Anhui Engineering Laboratory for Industrial Microbiology Molecular Breeding College of Biology and Food EngineeringAnhui Polytechnic UniversityWuhu 241000China

The production of androst-4-ene-3,17-dione(AD)by the steroidal microbial cell factory requires transcription factors(TFs)to participate in metabolic ***,microbial cell factory lacks effective TFs that can respond to AD in its metabolic ***,finding and obtaining natural TFs that specifically respond to AD is a complex and onerous *** this study,we devised an artificial TF that responds to AD,termed AdT,based on structure-guided molecular dynamics(md)*** to md analysis of the conformational changes of AdT after binding to AD,an LBD in which the N-and C-termini exhibited convergence tendencies was used as a microswitch to guide the assembly of a DNA-binding domain lexA,a linker(GGGGS)2,and a transcription activation domain B42 into an artificial *** a proof of design,a AD biosensor was designed and constructed in yeast on the basis of the ligand-binding domain(LBD)of hormone *** addition,the transcription factor activity of AdT was increased by 1.44-fold for its variant ***,we created non-natural TF elements for AD microbial cell factory,and expected that the design TF strategy will be applied to running in parallel to the signaling machinery of the host cell.

关键词： Protein design Artificial transcription factor Molecular dynamics Steriods Site-mutations

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The effectiveness of physical activity interventions on blood pressure in children and adolescents:A systematic review and network meta-analysis

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Journal of Sport and Health Science 2024年第5期13卷 699-708页

作者： Mohamed A.Hassan Wanjiang Zhou Mingyi ye Hui He Zan Gao School of Kinesiology University of Minnesota-Twin CitiesMinneapolisMN 55455USA Department of Methods and Curriculum Physical Education College for MenHelwan UniversityCairo 12552Egypt China Institute of Sport and Health Science Beijing Sport UniversityBeijing 100084China Department of Kinesiology Recreationand Sport StudiesUniversity of TennesseeKnoxvilleTN 37996USA

Background:High blood pressure(BP)is a major contributor to mortality and cardiovascular *** the known benefits of exercise for reducing BP,it is crucial to identify the most effective physical activity(PA)*** systematic review and network meta-analysis(NMA)aimed to evaluate the available evidence on the effectiveness of various PA interventions for reducing BP and to determine their hierarchy based on their impact on ***:A search of PubMed,SPORTDiscus,PsycINFO,Web of Science,CINAHL,Cochrane,and Eric databases was conducted up to December 2022 for this systematic review and *** controlled trials and quasi-experimental studies targeting healthy children and adolescents aged 6-12 years old were included in this *** studies that compared controlled and intervention groups using PA or exercise as the major influence were *** followed the Preferred Reporting Items for Systematic Reviews and Meta-Analyses(PRISMA)*** independent investigators performed the literature screening,data extraction,and risk of bias *** used Bayesian arm-based NMA to synthesize the *** primary outcomes were systolic BP and diastolic *** calculated the mean differences(mds)in systolic BP and diastolic BP before and after *** treatment differences were estimated using NMA and random-effect ***:We synthesized 27 studies involving 15,220 children and *** combined with nutrition and behavior change was the most effective intervention for reducing both systolic BP and diastolic BP(md=-8.64,95%credible interval(95%CI):-11.44 to-5.84;md=-6.75,95%CI:-10.44 to-3.11),followed by interventions with multiple components(md=-1.39,95%CI:-1.94 to-0.84;md=-2.54,95%CI:-4.89 to-0.29).Conclusion:Our findings suggest that PA interventions incorporating nutrition and behavior change,followed by interventions with multiple components,are most effective for reducing both systolic BP and diastolic BP

关键词： Children Diastolic blood pressure Physical activity Systolic blood pressure

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FOX-7在不同溶剂中的形貌预测研究

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兵器装备工程学报 2024年第5期45卷 167-172页

作者：张诗敏叶宝云王晶禹石宋超中北大学环境与安全工程学院太原030051

采用分子动力学(md)方法,预测了1,1-二氨基-2,2-二硝基乙烯(FOX-7)在真空条件下以及3种不同溶剂-非溶剂体系(DMSO-H_(2)O、DMSO-乙醇、DMAC-乙醇,溶剂与非溶剂体积比为1∶2)下的晶体形貌,确定真空条件下FOX-7的形态学重要生长面和附着能... 详细信息

采用分子动力学(md)方法,预测了1,1-二氨基-2,2-二硝基乙烯(FOX-7)在真空条件下以及3种不同溶剂-非溶剂体系(DMSO-H_(2)O、DMSO-乙醇、DMAC-乙醇,溶剂与非溶剂体积比为1∶2)下的晶体形貌,确定真空条件下FOX-7的形态学重要生长面和附着能,计算溶剂与晶面之间的相互作用能、溶剂影响下的晶面附着能。结果表明:FOX-7在真空中的主要生长面有6个面,分别为(0 1 1)、(1 0-1)、(1 0 1)、(0 0 2)、(1 1 0)、(1 1-1),其中(0 1 1)晶面面积最大,占比达到49.38%,是FOX-7晶体形态的最重要晶面。在DMSO-H_(2)O体系中形貌为方形,在DMSO-乙醇中晶体较扁,趋于圆形,在DMAC-乙醇中晶体形貌更为立体,趋于球形。对预测效果较好的DMAC-乙醇体系进行试验,得到的晶体形貌规整,为类球形,与模拟结果相符,说明了预测的准确性。

关键词： 1 1-二氨基-2 2-二硝基乙烯(FOX-7) 形貌预测分子动力学附着能结晶

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Hazard-based duration modelling of merging time interval on freeway on-ramps

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Transportation Safety and Environment 2023年第1期5卷 8-17页

作者： ye Li Jichen Zhu md Mazharul Haque Jaeyoung Lee School of Traffic and Transportation Engineering Central South UniversityChangsha 410075HunanChina Key Laboratory of Road and Traffic Engineering of Ministry of Education Tongji UniversityShanghai 201804China School of Civil and Environmental Engineering Queensland University of TechnologyBrisbaneQLD 4001Australia.

Freeway on-ramps suffer high crash risks due to frequent merging *** study developed hazard-based duration models to investigate the merging time interval on freeway on-ramps based on microscopic trajectory *** effect,random effect and random parameters Weibull distributed accelerated failure time models were developed to capture merging time as a function of various dynamic *** random parameters model was found to outperform the two counterparts since the unobserved heterogeneity of individual drivers was *** estimation results indicate that drivers along the merging section with an auxiliary lane perform a smooth merging process and are easily affected by speed *** of leading and following vehicles on the merging and target lanes are found to influence the merging time interval for merging without an auxiliary lane,whereas the influence of surrounding vehicles ismarginal for thosewith an auxiliary *** findings of this study identify potential countermeasures for improving safety during the merging process.

关键词： hazard-based duration model on-ramp merging trajectory data freeway safety

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