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Streamlined and on-demand preparation of mRNA products on a universal integrated platform

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Microsystems & Nanoengineering 2023年第4期9卷 119-128页

作者： Hongjuan Wei Zhen Rong Liyan Liu ye sang Jing Yang Shengqi Wang Bioinformatics Center of AMMS Beijing 100850P.R.China

Vaccines are used to protect human beings from various *** vaccines simplify the development process and reduce the production cost of conventional vaccines,making it possible to respond rapidly to acute and severe diseases,such as coronavirus disease *** this study,a universal integrated platform for the streamlined and ondemand preparation of mRNA products directly from DNA templates was *** DNA templates were amplified in vitro by a polymerase chain reaction module and transcribed into mRNA sequences,which were magnetically purified and encapsulated in lipid *** an initial example,enhanced green fluorescent protein(eGFP)was used to test the *** expression capacity and efficiency of the products were evaluated by transfecting them into HEK-293T *** batch production rate was estimated to be 200–300μg of eGFP mRNA in 8 ***,an mRNA vaccine encoding the receptor-binding domain(RBD)of the severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)spike protein was produced by this *** proposed integrated platform shows advantages for the universal and on-demand preparation of mRNA products,offering the potential to facilitate broad access to mRNA technology and enable the development of mRNA products,including the rapid supply of new mRNA-based vaccines in pandemic situations and personalized mRNA-based therapies for oncology and chronic infectious diseases,such as viral hepatitis and acquired immune deficiency syndrome.

关键词： preparation template offering

An mRNA vaccine with broad-spectrum neutralizing protection against Omicron variant sublineages BA.4/5 -included SARS-CoV-2

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Signal Transduction and Targeted Therapy 2022年第11期7卷 4011-4013页

作者： ye sang Zhen Zhang Entao Li Haitao Lu Jinrong Long Yiming Cao Changxiao Yu Tiecheng Wang Jing Yang Shengqi Wang Beijing Institute of Microbiology and Epidemiology Beijing100850China School of Life Science University of HebeiBaoding071002China Veterinary Research Institute Chinese Academy of Agricultural SciencesChangchun130000China

Dear Editor,The COVID-19 pandemic,caused by SARS-CoV-2,has resulted in more than 500 million confirmed cases,with over 6 million deaths by June *** the outbreak of the pandemic,five variants,including Alpha,Beta,Gamma,Delta,and Omicron,have been classified as *** a result of selection pressure and continued replication of the virus in human populations,new variants are sure to emerge,which will affect the spread of SARS-CoV-2 and vaccine efficacy.

关键词： vaccine neutral continued

Australia and China:A Joint Report on the Bilateral Relationship

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Contemporary International Relations 2012年第3期22卷 42-57页

作者： Liu Junhong Guo Chunmei Richard Rigby sang ye China Australia

After China and Australia established diplomatic ties in 1972, bilateral relations developed rapidly. People-to-people links, politicalexchanges and trade and economic cooperation played an increasingly larger part in the foreign policies of the two countries. In the post global financial crisis era, bilateral relations have remained positive and played an imPortant role in ensuring the stability and prosperity of the Pacific region. There have been several unavoidable misunderstandings and even disagreements between the two countries, which has influenced mutual trust and policies. This article will aim to analyze the history and current situation of the Sino-Australian relationship in an objective and comprehensive way; to look ahead to future bilateral relations, and make policy recommendations for the further development of bilateral ties to increase mutual understanding and promote a more positive future for Sino-Australian relations.

关键词：澳大利亚双边中国外交政策经济合作金融危机亚太地区

Clinical longitudinal evaluation of COVID-19 patients and prediction of organ-specific recovery using artificial intelligence

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Precision Clinical Medicine 2021年第1期4卷 62-69页

作者： Winston T.Wang Charlotte L.Zhang Kang Wei ye sang Jun Shen Guangyu Wang Alexander X.Lozano Department of Materials Science&Engineering Stanford UniversityStanfordCA 94305USA Mayo Clinic Alix school of Medicine ScottsdaleAZ 85259USA Guangzhou Women and Children’s Medical Center Guangzhou Medical UniversityGuangzhou 510623China The First College of Clinical Medical Science China Three Gorges UniversityYichang 443000China Department of Radiology Sun Yat-Sen Memorial HospitalSun Yat-Sen UniversityGuangzhou 510120China School of Information and Communication Engineering Beijing University of Posts and TelecommunicationsBeijing 100876China

Within COVID-19 there is an urgent unmet need to predict at the time of hospital admission which COVID-19 patients will recover from the disease,and how fast they recover to deliver personalized treatments and to properly allocate hospital resources so that healthcare systems do not become *** this end,we have combined clinically salient CT imaging data synergistically with laboratory testing data in an integrative machine learning model to predict organ-specific recovery of patients from *** trained and validated our model in 285 patients on each separate major organ system impacted by COVID-19 including the renal,pulmonary,immune,cardiac,and hepatic *** greatly enhance the speed and utility of our model,we applied an artificial intelligence method to segment and classify regions on CT imaging,from which interpretable data could be directly fed into the predictive machine learning model for overall *** all organ systems we achieved validation set area under the receiver operator characteristic curve(AUC)values for organ-specific recovery ranging from 0.80 to 0.89,and significant overall recovery prediction in Kaplan-Meier *** demonstrates that the synergistic use of an artificial intelligence(AI)framework applied to CT lung imaging and a machine learning model that integrates laboratory test data with imaging data can accurately predict the overall recovery of COVID-19 patients from baseline characteristics.

关键词： artificial intelligence medical image COVID-19 longitudinal evaluation

LAMOST/HRS spectroscopic analysis of two new Li-rich giants

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Research in Astronomy and Astrophysics 2021年第1期21卷 198-206页

作者： Ze-Ming Zhou Jian-Rong Shi Hong-Liang Yan Yong-Hui Hou Kai Zhang Qi Gao Xiao-Dong Xu Hai-Long Yuan Yu-Tao Zhou Kaike Pan Zi-ye sang Yong-Heng Zhao CAS Key Laboratory of Optical Astronomy National Astronomical ObservatoriesChinese Academy of ScienceBeijing 100101China School of Astronomy and Space Science University of Chinese Academy of SciencesBeijing 100049China Nanjing Institute of Astronomical Optics&Technology National Astronomical ObservatoriesChinese Academy of SciencesNanjing 210042China Department of Astronomy Peking UniversityBeijing 100871China Kavli Institute for Astronomy and Astrophysics Peking UniversityBeijing 100871China Apache Point Observatory SunspotNew Mexico 88349USA Astronomy Department New Mexico State UniversityLas CrucesNew Mexico 88003USA

Two Li-rich candidates,TYC 1338-1410-1 and TYC 2825-596-1,were observed by the new high-resolution echelle spectrograph,LAMOST/*** on their high-resolution and high-signal-tonoise ratio(SNR)spectra,we derived stellar parameters and abundances of 14 important elements for the two *** stellar parameters and lithium abundances indicate that they are Li-rich K-type giants,and having A(Li)NLTE and 2.91 dex,*** analysis suggests that TYC 1338-1410-1 is probably a red giant branch(RGB)star at the bump stage,while TYC 2825-596-1 is most likely to be a core helium-burning red clump(RC)*** line profiles of both spectra indicate that the two Li-rich giants are slow rotators and do not show infrared(IR)*** conclude that engulfment is not the lithium enrichment mechanism for either *** enriched lithium of TYC 1338-1410-1 could be created via the Cameron-Fowler mechanism,while the lithium excess in TYC 2825-596-1 could be associated with either non-canonical mixing processes or He-flash.

关键词： stars:fundamental parameters stars:late-type stars:low-mass stars:abundances stars:chemically peculiar

Monkeypox virus quadrivalent mRNA vaccine induces immune response and protects against vaccinia virus

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Signal Transduction and Targeted Therapy 2023年第5期8卷 2423-2433页

作者： ye sang Zhen Zhang Fan Liu Haitao Lu Changxiao Yu Huisheng Sun Jinrong Long Yiming Cao Jierui Mai Yiqi Miao Xin Wang Jiaxin Fang Youchun Wang Weijin Huang Jing Yang Shengqi Wang Bioinformatics center of AMMS Beijing100850P.R.China Division of HIV/AIDS and Sex-transmitted Virus Vaccines Institute for Biological Product ControlNational Institutes for Food and Drug Control(NIFDC)Beijing102629P.R.China Institute of Medical Biology Chinese Academy of Medical SciencesKunming650031P.R.China

Monkeypox has been declared a public health emergency by the World Health *** is an urgent need for efficient and safe vaccines against the monkeypox virus(MPXV)in response to the rapidly spreading monkeypox *** the age of COVID-19,mRNA vaccines have been highly successful and emerged as platforms enabling rapid development and large-scale ***,we develop two MPXV quadrivalent mRNA vaccines,named mRNA-A-LNP and mRNA-B-LNP,based on two intracellular mature virus specific proteins(A29L and M1R)and two extracellular enveloped virus specific proteins(A35R and B6R).By administering mRNA-A-LNP and mRNA-B-LNP intramuscularly twice,mice induce MPXV specific IgG antibodies and potent vaccinia virus(VACV)specific neutralizing ***,it elicits efficient MPXV specific Th-1 biased cellular immunity,as well as durable effector memory T and germinal center B cell responses in *** addition,two doses of mRNA-A-LNP and mRNA-B-LNP are protective against the VACV challenge in ***,the passive transfer of sera from mRNA-A-LNP and mRNA-B-LNP-immunized mice protects nude mice against the VACV ***,our results demonstrate that mRNA-A-LNP and mRNA-B-LNP appear to be safe and effective vaccine candidates against monkeypox epidemics,as well as against outbreaks caused by other orthopoxviruses,including the smallpox virus.

关键词： doses protective immunity

Deep learning method for determining the surface elastic moduli of microstructured solids

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Deep learning method for determining the surface elastic mod...

中国力学大会-2021+1

作者： sang ye Wei-Zhi Huang Min Li Xi-Qiao Feng Institute of Biomechanics and Medical Engineering AML and SKLTDepartment of Engineering MechanicsTsinghua University

Introduction Surface microstructures greatly affect many physical and mechanical properties of solid *** constitutive relations are often required to analyze the mechanical responses of materials and structures at the micro scales,but it remains a challenge to determine the surface constitutive parameters of microstructured materials.

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论10世纪拜占庭城市科尔松的重要经济作用

内蒙古大学学报（哲学社会科学版） 2020年第6期52卷 40-47页

作者：桑叶南开大学历史学院天津300350

科尔松是拜占庭帝国在黑海北岸、克里米亚统治区的沿海城市。在帝国马其顿王朝鼎盛时期的10世纪,帝国经济繁荣,商业兴盛,科尔松在帝国经济发展中起到了重要的推动作用。丰沛的渔业、盐业和良好的手工制造业使科尔松成为帝国重要的生产... 详细信息

科尔松是拜占庭帝国在黑海北岸、克里米亚统治区的沿海城市。在帝国马其顿王朝鼎盛时期的10世纪,帝国经济繁荣,商业兴盛,科尔松在帝国经济发展中起到了重要的推动作用。丰沛的渔业、盐业和良好的手工制造业使科尔松成为帝国重要的生产中心。且科尔松地处克里米亚半岛西南沿海,是周围航线的必经之地,优越的地理区位因素使科尔松成为帝国海洋贸易中的转运中心。在广阔而发达的经济腹地上,科尔松为帝国构建起一个连接南俄草原各个民族的陆地贸易区域中心。大量货币和商品流通为科尔松带来了巨大的经济收益,发达的经济使其在帝国历史上的经济地位不容小觑。

关键词：科尔松拜占庭帝国经济作用南俄大草原

“第一次罗斯远征君士坦丁堡”对拜占庭帝国的影响

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内蒙古大学学报（哲学社会科学版） 2021年第2期53卷 77-85页

作者：桑叶南开大学历史学院天津300350

"第一次罗斯远征君士坦丁堡"是罗斯有史以来发动的第一场针对拜占庭帝国的首都——君士坦丁堡及周边的军事行动。这场远征给拜占庭居民的生命财产造成了巨大的损失,拜占庭居民也遭受了严重的精神创伤,帝国损失惨重。由于这场远征的攻势... 详细信息

"第一次罗斯远征君士坦丁堡"是罗斯有史以来发动的第一场针对拜占庭帝国的首都——君士坦丁堡及周边的军事行动。这场远征给拜占庭居民的生命财产造成了巨大的损失,拜占庭居民也遭受了严重的精神创伤,帝国损失惨重。由于这场远征的攻势震惊了帝国,使帝国开始了解、认识并重视这支崛起于南俄大草原的新兴力量。这场远征还让拜占庭改变了以往的外交策略,凶残野蛮的罗斯人成为拜占庭实行宗教外交的新目标。"第一次罗斯受洗"标志着拜占庭基督教世界的版图再次扩大。此后,罗斯成为拜占庭的得力盟友,双方在多个方面建立了联系。

关键词：第一次罗斯远征君士坦丁堡拜占庭帝国基督教罗斯受洗