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Highβ-sitosterol-D-glucoside content in sweet potato varieties and its anti-breast cancer potential through multiple metastasis-associated signals

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Food Science and Human Wellness 2024年第5期13卷 2779-2789页

作者： Jinyin Zhang Lu Huang Xiang Tang Miao Pan xiaoli ye Xuegang Li Hang Ma School of Pharmaceutical Sciences Southwest UniversityChongqing 400716China School of Life Sciences Southwest UniversityChongqing 400715China

β-Sitosterol-D-glucoside(β-SDG)is a phytosterol compound whose antitumor activity has been confirmed by previous ***,its suppression on breast cancer remains *** that purpose,we isolatedβ-SDG from sweet potato and investigated the breast-cancer-inhibiting mechanism using proteomic *** sweet potato species S6 with highβ-SDG content were chosen form 36 species andβ-SDG was isolated by ***,an in situ animal model of breast cancer was established,andβ-SDG significantly reduced the tumor volume of MCF-7 xenograft *** analysis of tumor tissues revealed that 127 of these proteins were upregulated and 80 were *** ontology and network analysis showed that regulatory proteins were mainly associated with epithelial-mesenchymal transition(EMT),myogenesis,cholesterol homeostasis,oxidative phosphorylation and reactive oxygen pathways,while Vimentin,NDUF,VDAC1,PPP2CA and SNx9 were the most significant 5 node degree ***,in vitro and in vivo results showed that the protein expression of PPP2CA and Vimentin,which are markers of EMT,were involved in breast cancer cell metastasis and could be reversed byβ-*** work highlightsβ-SDG as a bioactive compound in sweet potato and the potential therapeutic effect ofβ-SDG for the treatment of breast cancer by inhibiting metastasis.

关键词： β-Sitosterol-D-glucoside(β-SDG) Sweet potato Breast cancer Proteomic Metastasis

Highly Efficient Hydrogenation of Methyl Propionate to Propanol over Hydrous Zirconia Supported Ruthenium

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Chinese Journal of Chemistry 2011年第2期29卷 229-236页

作者： Fan Guangyin Zhou Yafen Fu Haiyan ye xiaoli Li Ruixiang Chen Hua Li Xianjun Key Lab of Green Chemistry and Technology Ministry of Education College of Chemistry Sichuan University Chengdu Sichuan 610064 China College of Chemistry and Chemical Engineering China West Normal University Nanchong Sichuan 637002 China

Hydrous zirconia supported ruthenium catalyst Ru/ZrO2 xH2O, prepared by co-precipitating ruthenium trichloride and zirconium oxychloride with ammonia, was able to catalyze efficiently methyl propionate to propanol under the mild conditions. In aqueous system, the propanol yield of 〉99% was achieved under the conditions of reaction temperature of 150 ℃ and hydrogen pressure of 5.0 MPa, while in non-aqueous system the maximum propanol yield was only 47.0%. FTIR spectra and hydrogenation results indicated that the high catalytic performance of Ru/ZrO2 xH2O in aqueous phase results from the cooperation effect between water as a solvent and hydroxyl groups on the surtace of carrier.

关键词： ruthenium zirconium carboxylic acid ester alcohol hydrogenation

The frequency dependence of AC transport losses in stacked Bi—2223／Ag superconducting tapes

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Chinese Physics B 2003年第5期12卷 553-556页

作者： ZhangGuo-Min LinLiang-Zhen xiaoli-ye QiuMing YuYun-Jia HuiDong InstituteofElectricalEngineering ChineseAcademyofSciencesBeijing100080China

AC transport losses in a single superconducting tape, double- and triple-stacked Bi-2223/Ag superconducting tapes were measured by use of electrical method. The measurements were carried out at 77K with the frequency of AC transport currents ranging from 50 to 1000Hz. The dependence of AC losses on frequency and the number of tapes in the stack were presented and analysed.

关键词： AC losses stacked Bi-2223 tapes frequency dependence

Dorsomorphin induces cancer cell apoptosis and sensitizes cancer cells to HSP90 and proteasome inhibitors by reducing nuclear heat shock factor 1 levels

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Cancer Biology & Medicine 2019年第2期16卷 220-233页

作者： Na Li Ting Wang Zongmeng Li xiaoli ye Bo Deng Shu Zhuo Pengle Yao Mengmei Yang Hong Mei Xiaofang Chen Tengfei Zhu Shiting Chen Hui Wang Jiming Wang Yingying Le CAS Key Laboratory of Nutrition Metabolism and Food Safety Shanghai Institute of Nutrition and Health Shanghai/ Institutes for Biological SciencesUniversity of Chinese Academy of Sciences Chinese Academy of Sciences Shanghai 200031 China Key Laboratory of Food Safety Risk Assessment Ministry of Health Beijing 100022 China Cancer and Inflammation Program Center for Cancer Research National Cancer Institute at Frederick Frederick 21702 MD USA

Objective: Heat shock factor 1(HSF1), a transcriptional regulator of heat shock proteins(HSPs), is an attractive therapeutic target for cancer. However, only a few HSF1 inhibitors have been identified so ***: The mRNA and protein levels of HSF1, HSPs, cleaved PARP, and phosphorylated HSF1 were examined by real-time PCR and Western blot. Forced expression, RNA interference, and immunofluorescence assay were used for mechanistic *** viability and apoptosis were measured by WST-8 assay and flow cytometry, respectively. Xenograft studies were performed in nude mice to evaluate the effect of dorsomorphin and an HSP90 inhibitor on tumor ***: Dorsomorphin suppressed multiple stimuli-induced and constitutive HSPs expression in cancer cells. Mechanistic studies revealed that dorsomorphin reduced heat-induced HSP expression independent of adenosine monophosphate activated protein kinase. Dorsomorphin reduced heat-stimulated HSF1 Ser320 phosphorylation and nuclear translocation, as well as resting nuclear HSF1 levels in cancer cells. Dorsomorphin induced cancer cell apoptosis by inhibiting HSF1 expression. A structure-activity study revealed that the 4-pyridyl at the 3-site of the pyrazolo [1, 5-a]pyrimidine ring is critical for the anti-HSF1 activities of dorsomorphin. Dorsomorphin sensitized cancer cells to HSP90 and proteasome inhibitors and inhibited HSP70 expression induced by these inhibitors in vitro. In tumor-bearing nude mice, dorsomorphin enhanced HSP90 inhibitor-induced cancer cell apoptosis, tumor growth inhibition, and HSP70 ***: Dorsomorphin is an HSF1 inhibitor. It induces cancer cell apoptosis, sensitizes cancer cells to both HSP90 and proteasome inhibitors, and suppresses HSP upregulation by these drugs, which may prevent the development of drug ***, dorsomorphin and its derivates may serve as potential precursors for developing drugs against cancer.

关键词： Dorsomorphin heat shock factor 1 heat shock protein apoptosis cancer

A strategy with label-free quantification of the targeted peptides for quantitative peptidome analysis of human serum

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Science China Chemistry 2010年第4期53卷 759-767页

作者： ZHU Jun, WANG FangJun, DONG xiaoli, ye MingLiang & ZOU HanFa Key Laboratory of Separation Sciences for Analytical Chemistry National Chromatographic Research and Analysis Center Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China Key Laboratory of Separation Sciences for Analytical Chemistry Chinese Academy of Sciences Dalian China National Chromatographic Research and Analysis Center Chinese Academy of Sciences Dalian China Dalian Institute of Chemical Physics Chinese Academy of Sciences Dalian China

Peptidomics draws more and more attention in discovering useful biomarkers for early diagnosis of disease. However, there is lack of efficient quantification strategy in peptidome analysis. In this study, a strategy with label-free quantification of the targeted endogenous peptides based on peak intensity using μUPLC-Q-TOF-MS/MS was developed for quantitative peptidome analysis of human serum. Different amounts of standard BSA tryptic digesting peptides were added into the same serum extracts for evaluation of the developed strategy, and it was observed that the average relative error of the targeted peptides was 6.42%, which was superior to the result obtained directly by commercially available software PLGS. It was also demonstrated that this quantification strategy could obviously increase the detection sensitivity of the peptide by DDA analysis. Then, this strategy was applied to comparatively analyze the peptides extracted from the serum of HCC or breast cancer patients and healthy individuals, respectively. Peptides with charge states up to 5 and molecular weight over 4000 can be reliably identified and quantified. This quantitative analysis method based on μUPLC-Q-TOF-MS/MS exhibited superior sensitivity than that by MALDI-TOF-MS commonly used in peptidome analysis. Finally, some interesting endogenous peptides related to corresponding diseases were successfully obtained.

关键词： peptidomics label-free quantification hepatocellular carcinoma breast cancer human serum

美国大学协会本科STEM教育计划:理论框架与系统网络

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高教探索 2021年第9期 81-87页

作者：叶晓力安徽大学高等教育研究所合肥230039

美国大学协会(AAU)是美国高等教育领域最负盛名的社会组织之一,由62所世界一流研究型大学组成。2011年,AAU倡议开展本科STEM教育计划,致力于研究型大学本科STEM教育的系统性变革。为指导本科STEM教学改革,AAU开发了理论框架,框架以教学... 详细信息

美国大学协会(AAU)是美国高等教育领域最负盛名的社会组织之一,由62所世界一流研究型大学组成。2011年,AAU倡议开展本科STEM教育计划,致力于研究型大学本科STEM教育的系统性变革。为指导本科STEM教学改革,AAU开发了理论框架,框架以教学法为核心,以教学支持和文化变革为重要内容,旨在改进本科STEM教学的关键制度要素,从而影响研究型大学STEM教育文化。随着该计划的深入推进,AAU逐步构建了本科STEM教育的系统性交互网络,以8所大学为项目站点,建立并衍生了更大范围的STEM网络和社区等平台。规模化网络社区的拓展不仅扩大了该计划的影响力,而且成为本科STEM教育改革可持续发展的重要组织基础,对提高研究型大学本科STEM教育质量至关重要。

关键词：美国大学协会本科STEM教育计划理论框架 STEM网络

蔗糖脂肪酸酯调节大豆叶片蔗糖酶生物合成机理研究(英文)

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大豆科学 2005年第2期24卷 95-100,105页

作者：叶小利李学刚西南师范大学生命科学学院重庆400715 西南农业大学中心实验室重庆400716

2mmol/L的SFE能显著地提高大豆蔗糖酶的活力。SDS-PAGE结果表明SFE主要通过增加大豆叶片蔗糖酶的生物合成量来大幅度地提高蔗糖酶的活力。用转录抑制剂AMD(8mg/L)和翻译抑制剂CHI(1.5mg/L)处理大豆叶片,发现它们都能在较短时间内大幅度... 详细信息

2mmol/L的SFE能显著地提高大豆蔗糖酶的活力。SDS-PAGE结果表明SFE主要通过增加大豆叶片蔗糖酶的生物合成量来大幅度地提高蔗糖酶的活力。用转录抑制剂AMD(8mg/L)和翻译抑制剂CHI(1.5mg/L)处理大豆叶片,发现它们都能在较短时间内大幅度地降低蔗糖酶的活性。SFE对大豆开花期和结荚期的蔗糖酶生物合成量的增加是通过SFE促进蔗糖酶的转录水平和翻译水平来实现的,转录起主要作用。

关键词：大豆叶片蔗糖酶蔗糖脂肪酸酯机理研究生物合成 SDS-PAGE SFE 转录水平大幅度合成量抑制剂结荚期开花期活力翻译

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效率与民主之争:博比特与杜威课程观之比较

教育学报 2023年第5期19卷 39-50页

作者：叶晓力徐培鑫厦门大学教育研究院福建厦门361001 安徽大学高等教育研究所合肥230039

作为同一时代的教育家,博比特与杜威都曾深刻影响了美国公立学校的课程变革。博比特的社会效率取向课程观对美国20世纪初的学校课程发展产生了重要影响,但其伴随着经济危机的爆发趋于式微;而杜威的社会民主取向课程观则在经济危机后的... 详细信息

作为同一时代的教育家,博比特与杜威都曾深刻影响了美国公立学校的课程变革。博比特的社会效率取向课程观对美国20世纪初的学校课程发展产生了重要影响,但其伴随着经济危机的爆发趋于式微;而杜威的社会民主取向课程观则在经济危机后的课程重建中发挥了重要作用。在课程目标观上,博比特坚持“为成人生活做准备”,而杜威则强调“儿童经验的不断生长”;在课程内容观上,博比特重视协调直接经验与间接经验的结合,而杜威则更推崇直接经验的价值;在课程编制观上,博比特创造了一种基于社会逻辑的“活动分析法”,而杜威则更关注学生的身心发展规律,提出了“教材心理学化”;在课程实施观上,博比特指出只有通过大量的专门训练才能够实现预设目标,而杜威则强调“手脑并用”的亲身参与。重新审视博比特与杜威的课程观,尽管他们的课程主张存在诸多“立场对峙”,但其中亦不乏思想契合之处,如都主张教育为社会服务、都重视经验的积极作用、都遵循一定的科学逻辑以及都强调学习的亲身实践。

关键词：博比特杜威社会效率社会民主课程观

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氨水/Fe~Ⅱ EDTA溶液同时脱硫脱硝实验研究

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环境科学学报 2014年第6期34卷 1560-1566页

作者：叶小莉吴晓琴王淑娟武汉科技大学化学工程与技术学院武汉430081

在间歇鼓泡反应器中,采用FeⅡEDTA复配氨水对协同吸收SO2和NO进行了研究,研究过程中考察了NH3-NH+4的浓度、吸收液温度、初始pH值、络合剂浓度及种类对SO2和NO吸收效果的影响.结果表明,当NH3-NH+4的浓度小于2.1412 mol·L-1时,NO的脱除... 详细信息

在间歇鼓泡反应器中,采用FeⅡEDTA复配氨水对协同吸收SO2和NO进行了研究,研究过程中考察了NH3-NH+4的浓度、吸收液温度、初始pH值、络合剂浓度及种类对SO2和NO吸收效果的影响.结果表明,当NH3-NH+4的浓度小于2.1412 mol·L-1时,NO的脱除率随着NH3-NH+4浓度的增加而增大,当NH3-NH+4的浓度大于2.1412 mol·L-1以后,由于过多的硫酸根的影响,对NO的吸收有抑制作用;温度的升高不利于SO2和NO的吸收;随着pH值由小到大的变化,FeⅡEDTA在液相中的组成形态发生改变,其络合能力在pH为6.5时达到最大值;络合剂浓度增加,脱硝率随之增加.当FeⅡEDTA浓度增加到0.03 mol·L-1以后,脱硝率的增长趋势随着络合剂浓度的增大变缓;与氯化钴、高锰酸钾、三乙醇胺、尿素和氨水复配后的溶液吸收NO过程相比较,FeⅡEDTA络合剂对NO的脱除率明显高于其他添加剂,是最佳的氨水复配络合吸收剂.根据实验结果,本系统中最佳协同脱除SO2和NO的工艺条件为:NH3-NH+4浓度为2.1412 mol·L-1,吸收温度为50℃,pH为6.5和FeⅡEDTA浓度为0.03 mol·L-1.

关键词： FeⅡ EDTA 氨水脱硫脱硝