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Agrobacterium tumefaciens-mediated genetic transformation of the phytopathogenic fungus Penicillium digitatum

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2008年第10期9卷 823-828页

作者： Ji-ye WANG Hong-ye LI Institute of Biotechnology Zhejiang University Hangzhou 310029 China

Agrobacterium tumefaciens-mediated transformation (ATMT) system was assessed for conducting insertional mutagenesis in Penicillium digitatum, a major fungal pathogen infecting post-harvest citrus fruits. A transformation efficiency of up to 60 transformants per 106 conidia was achieved by this system. The integration of the hph gene into the fungal genome was verified by polymerase chain reaction (PCR) amplification and sequencing. These transformants tested were also shown to be mitotically stable. Southern blot analysis of 14 randomly selected transformants showed that the hph gene was randomly integrated as single copy into the fungal genome of P. digitatum. Thus, we conclude that ATMT of P. digitatum could be used as an alter-natively practical genetic tool for conducting insertional mutagenesis in P. digitatum to study functional genomics.

关键词： Penicillium digitatum Agrobacterium tumefaciens-mediated transformation (ATMT) Hygromycin B resistance gene lnsertional mutagenesis

Enhancing blocking temperature using inverse hydrogen bonds for non-radical bridged dimeric Dy(Ⅲ)single-molecule magnets

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Science China Chemistry 2024年第10期67卷 3328-3338页

作者： Peng-Bo Jin Qian-Cheng Luo ye-ye Liu Yan-Zhen Zheng Frontier Institute of Science and Technology State Key Laboratory of Electrical Insulation and Power EquipmentMOE Key Laboratory for Nonequilibrium Synthesis and Modulation of Condensed MatterXi’an Key Laboratory of Electronic Devices and Material Chemistry and School of ChemistryXi’an Jiaotong UniversityXi’an 710054China

Single-molecule magnets(SMMs)are a kind of nanosized magnetic materials that are capable of storing massive bytes of *** coupling the spin centers in a proper manner is a usual approach to promote the working temperature(or blocking temperature)for *** delocalized radicals have been widely employed to accomplish this ***,we show a new manner by using weak but multiple B–H^(δ-)···Dy^(3+)inverse hydrogen bonding(IHB)interactions to control the magnetic couplings in a series of dimeric dysprosiacaborane *** approach leads to a record high T_(B)^(100s)of 10 K among non-radical bridged dimeric SMMs,which is mainly ascribed to strong ferromagnetic coupling(4.38 cm^(-1))and the proper alignment of the magnetic principle axes of the adjacent dysprosium(Ⅲ)*** verifying by theoretical calculations,these results demonstrate that IHB interactions can be used to construct strong axial ferromagnetic coupling and enhancing magnetic blocking temperature for SMMs.

关键词： single-molecule magnets carborane dysprosium ferromagnetic coupling inverse hydrogen bond

Advances in epigenetic biomarker research in colorectal cancer

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World Journal of Gastroenterology 2014年第15期20卷 4276-4287页

作者： Xi Wang ye-ye Kuang Xiao-Tong Hu Biomedical Research Center Sir Run Run Shaw HospitalZhejiang University and Key Laboratory of Biotherapy of Zhejiang Province

Colorectal cancer(CRC)causes approximately 600000deaths annually and is the third leading cause of cancer mortality *** significant advancements in treatment options,CRC patient survival is still poor owing to a lack of effective tools for early diagnosis and a limited capacity for optimal therapeutic decision *** there exists a need to find new biomarkers to improve diagnosis of CRC,the research on epigenetic biomarkers for molecular diagnostics encourages the translation of this field from the bench to clinical *** alterations are thought to hold great promise as tumor *** this review,we will primarily focus on recent advances in the study of epigenetic biomarkers for colorectal cancer and discuss epigenetic biomarkers,including DNA methylation,microRNA expression and histone modification,in cancer tissue,stool,plasma,serum,cell lines and *** studies have improved the chances that epigenetic biomarkers will find a place in the clinical practices of screening,early diagnosis,prognosis,therapy choice and recurrence surveillance for CRC ***,these studies have typically been small in size,and evaluation at a larger scale of well-controlled randomized clinical trials is the next step that is necessary to increase the quality of epigenetic biomarkers and ensure their widespread clinical use.

关键词： Colorectal cancer Epigenetic biomarker DNA methylation MicroRNA biomarker Histone modification

Removal of Ocular Artifacts from Electroencephalo-Graph by Improving Variational Mode Decomposition

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China Communications 2022年第2期19卷 47-61页

作者： Miao Shi Chao Wang Wei Zhao Xinshi Zhang ye ye Nenggang Xie Department of Mechanical Engineering Anhui University of TechnologyAnhuiMa’anshan 243002PR China College of Civil Engineering and Architecture Anhui Polytechnic UniversityAnhuiWuhu 241000PR China Department of Computer Science and Technology Anhui University of TechnologyAnhuiMa’anshan 243002PR China Institute of Artificial Intelligence Hefei Comprehensive National Science CenterAnhuiHefei230000PR China Department of Management science and Engineering Anhui University of TechnologyAnhuiMa’anshan 243002PR China

Ocular artifacts in Electroencephalography(EEG)recordings lead to inaccurate results in signal analysis and *** Mode Decomposition(VMD)is an adaptive and completely nonrecursive signal processing *** are two parameters in VMD that have a great influence on the result of signal ***,this paper studies a signal decomposition by improving VMD based on squirrel search algorithm(SSA).It’s improved with abilities of global optimal guidance and opposition based *** original seasonal monitoring condition in SSA is *** feedback of whether the optimal solution is successfully updated is used to establish new seasonal monitoring ***-based learning is introduced to reposition the position of the population in this *** is applied to optimize the important parameters of ***-VMD model is established to remove ocular artifacts from EEG *** have verified the effectiveness of our proposal in a public dataset compared with other *** proposed method improves the SNR of the dataset from-2.03 to 2.30.

关键词： ocular artifact variational mode decomposition squirrel search algorithm global guidance ability opposition-based learning

Hydrothermal synthesis and crystal structure of copper （Ⅱ） coordination polymer composed of helix-like chains： [Cu（NIeH）（bpy）]

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Chinese Science Bulletin 2006年第14期51卷 1682-1686页

作者： ye Junwei ZHANG Ping ye Kaiqi ye Ling YANG Guangdi WANG Yue Key Laboratory for Supramolecular Structure and Materials Jilin University Changchun 130012 China

Hydrothermal reactions of Cu (II) ace-tate, 2,2′-bipyridyl (bpy) with 5-nitroisophthalic acid (H2NIPH) resulted in a new coordination polymer [Cu(NIPH)(bpy)] 1. Single crystal X-ray diffraction experiment indicates that 1 possesses a single helix- like chains, of which Cu atoms are coordinated by NIPH ligands and bpy ligands. Compound 1 crystal-lizes in the space group P2(1)/c, a = 0.955(19) nm, b = 1.259(3) nm, c = 1.3737(3) nm, β = 95.13(3)°, V = 1.6455(6) nm3 and Z = 4. The TGA analysis shows that 1 has no remarkable weight loss up to 284℃, as a result of its high thermal stability. Magnetic meas-urements indicate an antiferromagnetic behavior of compound 1.

关键词：超分子配位聚合物螺旋状链铜水热合成晶体结构

A New Type of Evolutionary Strategy Based on a Multi-player Iterated Prisoner’s Dilemma Game

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Journal of Systems Science and Information 2020年第1期11卷 67-81页

作者： Nenggang XIE ye ye Wei BAO Meng WANG School of Management Science and Engineering Anhui University of TechnologyMa’anshan 243002China School of Mechanical Engineering Anhui University of TechnologyMa’anshan 243002China School of Business Anhui University of TechnologyMa’anshan 243002China

According to the philosophy of self-cultivation that"one should refine his personal virtue when in poverty,and help save the world when in success",a new type of evolutionary strategy,PoorCompetition-Rich-Cooperation(PCRC),is *** discuss its superiority and inferiority,based on a multi-player iterated Prisoner’s Dilemma game,PCRC and other six kinds of strategies are played by using the roulette method in three different populations(a uniformly distributed population,a cooperation-preference population,a defection-preference population).The payoff characteristics for each strategy under different temptation coefficients and noise values are also *** results indicate that PCRC has a sufficient robustness and its payoff presents a basically monotonic increasing tendency with the increment of *** superiority of PCRC becomes more obvious when the temptation coefficient becomes ***,a higher population preference for defection yields a more obvious advantage for PCRC.

关键词： game Prisoner’s Dilemma PCRC robustness

Effects of appendectomy and oral tolerance on dextran sulfate sodium colitis

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World Journal of Gastroenterology 2011年第19期17卷 2437-2445页

作者： Min Yue Zhe Shen Chao-Hui Yu Hua ye Yue-Fang ye You-Ming Li Department of Gastroenterology The First Affili- ated Hospital Medical School Zhejiang University Hangzhou 310003 Zheiian~ Province China

AIM： To evaluate the concomitant effects of appendec- tomy and oral tolerance on ***： Delayed-type hypersensitivity （DTH） was investigated at a 7-d interval after ovalbumin （OVA） ad- ministration and immunization under normal and colitis conditions in appendectomized or sham-operated mice. Pathological scores for the colon were graded after in- gestion of colon-extracted protein （CEP） and induction of dextran sulfate sodium （DSS） colitis in appendecto- mized or sham-operated mice. Thereafter, Thl and Th2 in Peyer＇s patches and spleen lymphocytes were de- tected in CEP-treated and bovine serum albumin （BSA）-treated control ***： In appendectomized mice, DTH was not inhibited at day 7 after OVA administration and at the initial phase of DSS colitis, whereas it was inhibited at day 14 and day 21. However, in sham-operated mice, it was inhibited during the whole procedure and the onset of DSS colitis. The protective role of CEP against DSScolitis was present in sham-operated mice, with pre- dominant improvement of colonic pathological changes, while vanished in the appendectomized mice. A shift from Thl to Th2 in Peyer＇s patches resulted from a de- crease of Thl cells with the ingestion of CEP. Compared with BSA in the sham-operated group, no predominant changes were observed in the appendectomized ***： Appendectomy interferes with the pro- tective role of CEP in DSS colitis via a shift from Th2 to Thl during oral tolerance induction.

关键词： Appendicectomy Oral tolerance Dextrin sulfate sodium Murine colitis Th1-Th2 balance

Glacial Refugia of Ginkgo biloba and Human Impact on Its Genetic Diversity:Evidence from Chloroplast DNA

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Journal of Integrative Plant Biology 2008年第3期50卷 368-374页

作者： Wei Gong Zhen Zeng ye-ye Chen Chuan Chen Ying-Xiong Qiu Cheng-Xin Fu Laboratory of Systematic Evolutionary Botany and Biodiversity and State Conservation Center for Gene Resources of Endangered Wildlife College of Life Sciences Zhejiang University Hangzhou 310058 China

Variations in the trnK region of chloroplast DNA were investigated in the present study using polymerase chain reactionrestriction fragment length polymorphism to detect the genetic structure and to infer the possible glacial refugia of Ginkgo biloba L. in China. In total, 220 individuals from 12 populations in China and three populations outside China were analyzed, representing the largest number of populations studied by molecular markers to date. Nineteen haplotypes were produced and haplotype A was found in all populations. Populations in south-western China, including WC, JF, PX, and SP, contained 14 of the 19 haplotypes and their genetic diversity ranged from 0.771 4 to 0.867 6. The TM population from China also showed a high genetic diversity （H = 0.848 5）. Most of the genetic variation existed within populations and the differentiation among populations was low （GsT = 0.2）. According to haplotype distribution and the historical record, we suggest that populations of G. biloba have been subjected to extensive human impact, which has compounded our attempt to infer glacial refugia for Ginkgo. Nevertheless, the present results suggest that the center of genetic diversity of Ginkgo is mainly in south-western China and in situ conservation is needed to protect and preserve the genetic resources.

关键词： chloroplast DNA Ginkgo biloba glacial refugia human activities polymerase chain reaction-restriction fragment length polymorphism

Strong Antiferromagnetic Exchange-Coupling Observed in Hydride-Bridged Dimeric Dysprosium(Ⅲ)Single-Molecule Magnet

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Chinese Journal of Chemistry 2024年第4期42卷 391-396页

作者： Qian-Cheng Luo Ke-Xin Yu Peng-Bo Jin ye-ye Liu Yuan-Qi Zhai Yan-Zhen Zheng Frontier Institute of Science and Technology State Key Laboratory of Electrical Insulation and Power EquipmentXi'an Key Laboratory of Electronic Devices and Materials ChemistrySchool of Chemistry and School of PhysicsXi'an Jiaotong University99 Yanxiang RoadXi'anShaanxi710054 China

One dihydride-bridged dimeric Dy(Ⅲ)guanidinate complex,formulated as[{(Me_(3)Si)_(2)NC(NiPr)_(2)}_(2)Dy(μ-H)]_(2)(1Dy),was successfully isolated and the introduction of hydride bridges significantly reduces the intramolecular Dy(Ⅲ)…Dy(Ⅲ)distance to only 3.688(1)Å.To investigate the effect of such a short Dy(Ⅲ)…Dy(Ⅲ)distance on magnetism,we also prepared its dibromide-bridged analogue[{(Me_(3)Si)_(2)NC(NiPr)_(2)}_(2)Dy(μ-Br)]_(2)(2Dy),which has a much longer Dy(Ⅲ)…Dy(Ⅲ)distance of 4.605(4)Å.Surprisingly,2Dy demonstrates much larger effective energy barrier for magnetization reversal(U_(eff))and higher blocking temperature(T_(B)).The worse performance of 1Dy is attributed to the concerted effect of strong antiferromagnetic interactions between Dy(Ⅲ)ions(J_(total)=–2.683 cm^(–1))and the unparallel arrangement of magnetic principle axes of the Dy(Ⅲ)ions for 1Dy.

关键词： Single-molecule magnets Lanthanides Guanidinate ligand Hydride bridge Magnetic properties Ab initio calculations