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普萘洛尔通过长链非编码RNA核旁丛组装转录本1靶向微小RNA-194-5p调控血管瘤内皮细胞增殖

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中华实验外科杂志 2024年第8期41卷 1716-1720页

作者：王慧明徐伟立胡志刚安雯婷李萌马亚贞孙驰河北医科大学第二医院小儿外科石家庄050004 河北医科大学第二医院普外科石家庄050004 河北医科大学第二医院中心实验室石家庄050004

目的探讨普萘洛尔通过长链非编码RNA核旁丛组装转录本1(lncRNA NEAT1)靶向微小RNA(miR)-194-5p调控血管瘤内皮细胞(HemECs)增殖的作用。方法收集2021年3月至2022年8月在河北医科大学第二医院小儿外科手术切除的血管瘤标本20例,根据Mulli... 详细信息

目的探讨普萘洛尔通过长链非编码RNA核旁丛组装转录本1(lncRNA NEAT1)靶向微小RNA(miR)-194-5p调控血管瘤内皮细胞(HemECs)增殖的作用。方法收集2021年3月至2022年8月在河北医科大学第二医院小儿外科手术切除的血管瘤标本20例,根据Mulliken标准分为增生期组和消退期组。采用实时定量反转录聚合酶链反应(RT-qPCR)方法检测两组瘤体中NEAT1和miR-194-5p水平。制备HemECs,细胞计数试剂盒(CCK-8)试验检测敲低和过表达NEAT1对HemECs增殖影响。双荧光素酶酶报告基因实验及RT-qPCR验证miR-194-5p与NEAT1之间的相互作用。不同浓度普萘洛尔给药后,CCK-8试验检测细胞活性,5-乙炔基-2’脱氧尿嘧啶核苷(EdU)法检测细胞增殖,RT-qPCR方法检测NEAT1和miR-194-5p水平。两组间比较采用独立样本t检验进行分析,多组间比较采用单因素方差分析和重复测量方差分析,采用Spearman分析确定各指标间相关性。结果RT-qPCR结果显示增生期血管瘤NEAT1表达高于退化期血管瘤(17.43±5.38比8.82±3.70,t=4.176,P<0.01);而增生期血管瘤miR-194-5p表达低于退化期血管瘤(0.28±0.11比0.41±0.11,t=2.692,P<0.01)。CKK-8检测结果显示,si-NEAT1组细胞活性低于si-NC组(0.86±0.05比1.11±0.03,t=7.794,P<0.01);OE-NEAT1组细胞活性高于OE-NC组(1.33±0.01比1.11±0.05,t=8.072,P<0.05)。Si-NEAT1组miR-194-5p表达水平高于si-NC组(2.88±0.33比1.14±0.47,t=5.206,P<0.01);OE-NEAT1组miR-194-5p表达水平低于OE-NC组(0.43±0.06比1.24±0.11,t=10.830,P<0.01)。双荧光素酶报告基因实验结果显示miR-194-5p和NEAT1-WT共转染组荧光素酶活性高于miR-NC和NEAT1-WT共转染组(0.35±0.05比0.65±0.04,t=5.273,P<0.01)。OE-NEAT1+普萘洛尔组细胞EdU染色阳性细胞率显著低于OE-NEAT1组和OE-NC组(35.97±1.09比69.66±2.85、51.89±1.09,F=65.100,P<0.01),OE-NEAT1+普萘洛尔组NEAT1、miR-194-5p表达量均与OE-NEAT1组比较,差异有统计学意义(1.63±0.30比3.94±0.50,t=6.851,P<0.01;0.86±0.26比0.37±0.03,t=3.213,P<0.05)。结论普萘洛尔可通过NEAT1靶向miR-194-5p调节HemECs增殖,NEAT1可能作为婴幼儿血管瘤分子治疗的潜在靶点。

关键词：普萘洛尔长链非编码RNA 微小RNA 血管瘤内皮细胞增殖

Myc inhibition tips the immune balance to promote antitumor immunity

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Cellular & Molecular Immunology 2022年第9期19卷 1030-1041页

作者： Chao Yang Yun Liu Yudi hu Liang Fang Zhe huang huanhuan Cui Jun Xie yazhen Hong Wei Chen Nengming Xiao Qiyuan Li Wen-Hsien Liu Changchun Xiao State Key Laboratory of Cellular Stress Biology School of Life SciencesFaculty of Medicine and Life SciencesXiamen UniversityXiamen361102FujianChina Institute of Hematology School of MedicineXiamen UniversityXiamen361102FujianChina Shenzhen Key Laboratory of Gene Regulation and Systems Biology School of Life Sciences and Academy for Advanced Interdisciplinary StudiesSouthern University of Science and TechnologyShenzhen518005GuangdongChina Sanofi Institute for Biomedical Research Suzhou215123JiangsuChina Department of Immunology and Microbiology The Scripps Research InstituteLa JollaCA92037USA

Aberrant expression of Myc is one of the most common oncogenic events in human *** of Myc inhibitors are currently under development for treating Myc-driven *** addition to directly targeting tumor cells,Myc inhibition has been shown to modulate the tumor microenvironment to promote tumor ***,the effect of Myc inhibition on immune cells in the tumor microenvironment remains poorly ***,we show that the adaptive immune system plays a vital role in the antitumor effect of pharmacologic inhibition of *** genetic and pharmacologic approaches,we found that Myc inhibition enhanced CD8 T cell function by suppressing the homeostasis of regulatory T(Treg)cells and the differentiation of resting Treg(rTreg)cells to activated Treg(aTreg)cells in ***,we demonstrated that different Myc expression levels confer differential sensitivity of T cell subsets to pharmacologic inhibition of *** ablation of the Myc gene has been shown to suppress CD8 T cell function,Treg cells,which express much less Myc protein than CD8 T cells,are more sensitive to Myc *** differential sensitivity of CD8 T and Treg cells to Myc inhibitors resulted in enhanced CD8 T cell function upon Myc *** findings revealed that Myc inhibitors can induce an antitumor immune response during tumor progression.

关键词： Myc inhibition Regulatory T cells Differential sensitivity Antitumor immunity Cancer immunotherapy

Hybrid speciation via inheritance of alternate alleles of parental isolating genes

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Molecular Plant 2021年第2期14卷 208-222页

作者： Zefu Wang Yuanzhong Jiang Hao Bi Zhiqiang Lu yazhen Ma Xiaoyue Yang Ningning Chen Bin Tian Bingbing Liu Xingxing Mao Tao Ma Stephen P.DiFazio Quanjun hu Richard J.Abbott Jianquan Liu Key Laboratory for Bio-Resource and Eco-Environment of Ministry of Education College of Life Sciences&State Key Lab of Hydraulics&Mountain River EngineeringSichuan UniversityChengdu 610065China State Key Laboratory of Grassland Agro-Ecosystem Innovation Institute of Ecology and Life SciencesLanzhou UniversityLanzhou 730000China CAS Key Laboratory of Tropical Forest Ecology Xishuangbanna Tropical Botanical GardenChinese Academy of SciencesMengla 666303China Key Laboratory of Biodiversity Conservation in Southwest China State Forestry and Grassland AdministrationSouthwest Forestry UniversityKunming 650224China Institute of Loess Plateau Shanxi UniversityTaiyuan 030006China Department of Biology West Virginia UniversityMorgantownWV 25606USA School of Biology University of St AndrewsSt Andrews KY169THUK

It is increasingly realized that homoploid hybrid speciation(HHS),which involves no change in chromosome number,is an important mechanism of *** will likely increase in frequency as ecological and geographical barriers between species are continuing to be disrupted by human *** requires the establishment of reproductive isolation between a hybrid and its parents,but the underlying genes and genetic mechanisms remain largely *** this study,we reveal by integrated approaches that reproductive isolation originates in one homoploid hybrid plant species through the inheritance of altemate alleles at genes that determine parental premating *** parent species of this hybrid species are reproductively isolated by differences in flowering time and survivorship on soils containing high concentrations of *** found that the hybrid species inherits alleles of parental isolating major genes related to flowering time from one parent and alleles of major genes related to iron tolerance from the other *** this way,it became reproductively isolated from one parent by the difference in flowering time and from the other by habitat adaptation(iron tolerance).These findings and further modeling results suggest that HHS may occur relatively easily via the inheritance of alternate parental premating isolating genes and barriers.

关键词： hybrid speciation genetic mechanism reproductive isolation alternate alleles parental species

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重度子痫前期产前风险评估及临床意义

实用妇产科杂志 2011年第11期27卷 856-859页

作者：林雅真胡继芬厦门大学附属第一医院福建厦门361003 福建医科大学附属第一医院福建福州350003

目的:探讨重度子痫前期产前风险评估的方法及临床意义。方法:回顾性分析我院收治的152例重度子痫前期患者的临床资料,根据改良子痫前期风险评估方法评分,将患者分为A组(评分≤6分)71例、B组(6分0.05),C组分别与A组、B组比较,差异有高度... 详细信息

目的:探讨重度子痫前期产前风险评估的方法及临床意义。方法:回顾性分析我院收治的152例重度子痫前期患者的临床资料,根据改良子痫前期风险评估方法评分,将患者分为A组(评分≤6分)71例、B组(6分0.05),C组分别与A组、B组比较,差异有高度统计学意义(P<0.01);有并发症的孕妇其新生儿窒息率、FGR发生率和围生儿死亡率均高于无并发症的孕妇(P<0.05)。结论:重度子痫前期的风险评分越高、病情越重,但其病情呈一定的发展过程,仅病情进展到一定程度才影响母婴预后。重视对患者病情的整体风险评估对临床治疗及选择合适分娩时机有积极意义。

关键词：子痫前期重度风险评估分娩

Pervasive hybridization during evolutionary radiation of Rhododendron subgenus Hymenanthes in mountains of southwest China

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National Science Review 2022年第12期9卷 50-60页

作者： yazhen Ma Xingxing Mao Ji Wang Lei Zhang Yuanzhong Jiang Yuying Geng Tao Ma Liming Cai Shuangquan huang Pete Hollingsworth Kangshan Mao Minghui Kang Yiling Li Wenlu Yang Haolin Wu Yang Chen Charles C.Davis Nawal Shrestha Richard H.Ree Zhenxiang Xi Quanjun hu Richard I.Milne Jianquan Liu Key Laboratory for Bio-Resource and Eco-Environment of Ministry of Education College of Life SciencesSichuan University State Key Laboratory of Grassland Agro-ecosystem College of EcologyLanzhou University Department of Organismic and Evolutionary Biology and Harvard University Herbaria Harvard University Institute of Evolution and Ecology School of Life SciencesCentral China Normal University Royal Botanic Garden Edinburgh Negaunee Integrative Research Center Field Museum Institute of Molecular Plant Sciences The University of Edinburgh

Radiations are especially important for generating species biodiversity in mountainous *** contribution of hybridization to such radiations has rarely been ***,we use extensive genomic data to test whether hybridization was involved in evolutionary radiation within Rhododendron subgenus Hymenanthes,whose members show strong geographic isolation in the mountains of southwest *** sequenced genomes for 143 species of this subgenus and 93 species of four other subgenera,and found that Hymenanthes was monophyletic and radiated during the late Oligocene to middle *** hybridization events were inferred within and between the identified clades and *** suggests that hybridization occurred both early and late during diversification of subgenus Hymenan thes,although the extent to which hybridization,speciation through mixing-isolation-mixing or hybrid speciation,accelerated the diversification needs further *** of isolation and contact in such and other montane ecosystems may have together promoted species radiation through hybridization between diverging populations and *** radiation processes may apply to other montane floras in this region and elsewhere.

关键词： evolutionary radiation hybridization rhododendron subgenus Hymenanthes montane flora

基于瞬态面波法的道路地下病害无损探测技术应用

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岩土工程学报 2023年第S1期45卷 189-192页

作者：胡智尹方东王金昌孙雅珍浙江省交通运输科学研究院浙江省道桥检测与养护技术研究重点实验室浙江杭州310023 沈阳建筑大学交通与测绘工程学院辽宁沈阳110168 浙江大学建筑工程学院浙江杭州310058

针对杭州市某道路产生塌陷和裂缝的问题,利用瞬态面波法开展道路地下病害无损探测。根据地基波速试验探测结果分析不同地层情况,确定道路地下病害范围,为处理道路安全隐患提供了及时准确的数据资料。探测结果表明,道路下方7 m范围内存... 详细信息

针对杭州市某道路产生塌陷和裂缝的问题,利用瞬态面波法开展道路地下病害无损探测。根据地基波速试验探测结果分析不同地层情况,确定道路地下病害范围,为处理道路安全隐患提供了及时准确的数据资料。探测结果表明,道路下方7 m范围内存在土体疏松病害,给出的建议为部分区域进行注浆加固,部分区域清除表面碎石;瞬态面波法图像直观、经济快速,能够确定出具有工程灾害隐患的病害,为道路的检修和维护提供充分的科学依据。

关键词：无损检测瞬态面波测线道路病害

超声引导下高渗葡萄糖注射对于距腓前韧带慢性损伤的疗效研究

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空军航空医学 2024年第4期41卷 331-334页

作者：廖东初毛佶胡泽莹王明峰吴志彬李鹭朱俊杰吴洪光王玉琪毛龙飞孙熙洋王雅珍陆军厦门特勤疗养中心康复科厦门361002 南部战区总医院超声诊断科广州510000

目的研究超声引导下高渗葡萄糖注射对于距腓前韧带慢性损伤患者的治疗效果,为临床保守治疗距腓前韧带慢性损伤提供相关经验。方法选取单侧踝关节扭伤慢性疼痛患者60例,将患者随机分为A组、B组,A组注射高渗葡萄糖治疗,B组行超短波联合... 详细信息

目的研究超声引导下高渗葡萄糖注射对于距腓前韧带慢性损伤患者的治疗效果,为临床保守治疗距腓前韧带慢性损伤提供相关经验。方法选取单侧踝关节扭伤慢性疼痛患者60例,将患者随机分为A组、B组,A组注射高渗葡萄糖治疗,B组行超短波联合肌内效贴布治疗,分别评估患者治疗前与治疗后1、3个月和6个月的距腓前韧带超声表现、视觉模拟评分(Visual Analogue Scale,VAS)和美国足踝外科医师协会(American Orthopedic Foot and Ankle Society,AOFAS)后足评分。结果 2组治疗期间VAS评分结果显示,时间效应、组间效应和交互作用差异均有统计学意义(F组别=34.276,F时间=369.061,F交互=23.759,P均<0.001)。2组治疗期间AOFAS评分结果显示,时间效应、组间效应和交互作用差异均有统计学意义(F组别=97.236,F时间=322.951,F交互=11.175,P均<0.001)。A组治疗后1、3个月和6个月的VAS评分均较前改善,差异具有统计学意义(P均<0.001);A组治疗后1、3个月和6个月的AOFAS评分均较前改善,差异具有统计学意义(P均<0.001);B组治疗后1、3个月和6个月的VAS评分均较前改善,差异具有统计学意义(P均<0.001);B组治疗后1、3个月和6个月的AOFAS评分均较前改善,差异具有统计学意义(P均<0.001);治疗后1个月、3个月、6个月,A组VAS分数均比B组低,且差异有统计学意义(P=0.010、<0.001、<0.001);治疗后1、3个月和6个月,A组AOFAS分数均比B组高,且差异有统计学意义(P均<0.001)。结论对于距腓前韧带慢性损伤患者,超声引导下行高渗葡萄糖注射治疗安全、有效,值得临床进一步推广。

关键词：高渗葡萄糖距腓前韧带增生疗法超短波肌内效贴

MXene-mediated regulation of local electric field surrounding polyoxometalate nanoparticles for improved lithium storage

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Science China Materials 2022年第11期65卷 2958-2966页

作者： huixia Chao Yanan Li Yukun Lu yazhen Yao Yifan Zhu Hao Yang Kai Wang Yi Wan Qian Xu Lu Guan Han hu Mingbo Wu State Key Laboratory of Heavy Oil Processing Institute of New EnergyCollege of Chemical EngineeringChina University of Petroleum(East China)Qingdao 266580China College of Electrical Engineering Qingdao UniversityQingdao 266071China

Due to their capability of reversibly accepting multi lithium ions,polyoxometalates(POMs)have been widely regarded as promising candidates for electrochemical lithium ***,the insulating nature of POMs hinders fast migration kinetics of lithium within the bulk of these ***,we propose the introduction of a local electric field surrounding the POM nanoparticles consisting of Mn and V where the concomitant Coulomb forces can accelerate the migration of lithium *** rationally hybridizing POMs with MXene nanosheets,the imbalanced charge distribution emerging at their interface produces the local electric field,thereby leading to a 250-fold increase of lithium diffusion *** this regard,a capacitive contribution as high as 81.7%at 1.0 mV sis ***,the POM nanoparticles could densely assemble on the surface of MXene nanosheets,offering highly packed electrodes and thus high volumetric *** to the improved lithiumion transfer kinetics,the POMs/MXenes composites are paired with activated carbon to produce lithium-ion capacitors which could offer a high energy density of 195.5 W h kgand a large power capability of 3800 W *** findings in this work could build a clear relationship between materials with different conductivities for designing electrode materials.

关键词： local electric field polyoxometalate nanoparticles MXenes lithium storage

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面粉糊化特性与鲜湿及煮后面条质构特性关系

中国粮油学报 2005年第6期20卷 12-14,24页

作者：宋亚珍闫金婷胡新中西北农林科技大学农学院陕西杨凌712100 西安市优质农产品服务中心陕西西安710061 西北农林科技大学食品科学与工程学院陕西杨凌712100

为了研究小麦面粉糊化特性对于面条加工品质和食用品质的影响,选用了黄淮冬麦区(陕西省、河南省)的25个主栽小麦品种及2个专用面粉(新疆拉条子粉、日本乌东专用粉)为材料,研究了面粉糊化特性与鲜面条加工特性(面条长度、面条厚度、鲜面... 详细信息

为了研究小麦面粉糊化特性对于面条加工品质和食用品质的影响,选用了黄淮冬麦区(陕西省、河南省)的25个主栽小麦品种及2个专用面粉(新疆拉条子粉、日本乌东专用粉)为材料,研究了面粉糊化特性与鲜面条加工特性(面条长度、面条厚度、鲜面条最大拉伸阻力、拉伸长度和拉伸能量)、面条煮后品质(TPA测试、表面韧性、剪切韧性和压缩特性)的关系。研究结果认为对于鲜面条加工而言,低谷粘度、最终粘度、回生值、破损值、峰值时间和峰值温度贡献较大;对于煮后面条质构而言,最终粘度、回生值、破损值、峰值时间和峰值温度更为重要。总之,小麦品种的最终粘度、回生值、破损值对于面条加工和食用品质影响很大。

关键词：小麦淀粉糊化特性面条品质