检索结果-南通市图书馆

The peritoneum of fish expresses a specific gene pattern

Aquaculture and Fisheries 2024年第4期9卷 573-580页

作者： Yaoji He yawei shen Jinliang Zhao Xiaowu Chen Shanghai Ocean University National Experimental Science and Education Demonstration Center for Fishery ScienceShanghai201306China Shanghai Ocean University Shanghai Collaborative Innovation Center for Aquatic Animal Genetics and BreedingShanghai201306China

The peritoneum is mainly composed of mesothelial cells,fiber cells,and fibers,with many free cells that come from tissue *** chuatsi is a valuable fish commodity and important freshwater aquaculture species in *** the increase in the scale of its breeding,bacterial and viral disease of the *** has become increasingly rampant in recent *** study analyzed the peritoneal gene expression profile using transcriptome technology for the first *** results showed that 95,711 unigenes were expressed in the peritoneum with an average length of 920.72 *** addition to the housekeeping genes,the genes of extracellular matrix protein 1a(ecm1),Angiopoietin-related protein 4-like(angptl4)and cysteine-rich protein 1(crip1)were highly expressed genes in the peritoneum,and they are key factors in peritoneal development and the maintenance of ***,many immune genes were also expressed in the peritoneum,including beta-2-microglobulin(b2m),MHC II invariant chain(cd74),prostate stem cell antigen-like protein(psca),claudin(cldn),heat shock protein 90(hsp90),complement component 4(c4),radical S-adenosyl methionine domain-containing protein 2(rsad2),and cathepsin S(ctss).The RT-PCR results showed that different genes exhibited different expression *** transcriptome data indicated that b2m,cldn,hsp90,c4,and rsad2 were highly expressed in the peritoneum.b2m,cd74,psca,c4,and ctss were highly expressed after embryonic hatching,whereas cldn,hsp90,and rsad2 were expressed at early embryonic *** PCR demonstrated that b2m,cd74,cldn,hsp90,c4,and ctss genes were highly expressed after *** study provides significant data for studying the morphological and functional diversity of fish peritoneum and lays a foundation for research on mandarin fish immunity.

关键词： Siniperca chuatsi Peritoneum Transcriptome

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

microRNA expression profile of fish erythrocytes

引用

Aquaculture and Fisheries 2021年第6期6卷 558-564页

作者： Ziwei Zhao yawei shen Jinliang Zhao Xiaowu Chen National Demonstration Center for Experimental Fisheries Science Education(Shanghai Ocean University) Shanghai201306China Shanghai Engineering Research Center of Aquaculture Shanghai201306China Shanghai Collaborative Innovation for Aquatic Animal Genetics and Breeding Shanghai201306China

MicroRNAs(miRNAs)are short noncoding sequences that can regulate the expression of target ***,also called red blood cells(RBCs),are the largest proportion of cells in *** deliver oxygen in the body of vertebrates and contribute to the body’s immune *** sequencing technology was used to analyze pooled RNA samples isolated from erythrocytes,in order to characterize their gene expression profile and increase the range of miRNAs identified in Nile tilapia *** study identified conserved(n=309)and novel(n=194)*** reverse-transcription polymerase chain reaction and next-generation sequencing results indicated that miR-451 was the most abundant in RBCs,followed by let-7a-1,miR-2188 and ***,the tissue expression mode of these highly expressed miRNAs was *** show that miR-451,miR-2188,and miR-144 were all highly expressed in RBCs but were low abundance in other ***-1 and miR-10b were also abundantly expressed in muscle,while miR-122 was abundantly expressed in the ***-7a-1,miR-10b,and miR-1 were significantly higher in the testis than in the *** tissue distribution of miRNAs implies that these noncoding sequences have a potential role in regulating tissue differentiation or maintaining tissue *** results of a dual-luciferase reporter assay indicated that miR-144 and miR-10b inhibited the expression of the meningioma expressed antigen 5 gene in *** study contributes to the Nile tilapia miRNA database and reveals the profile of erythrocyte miRNA expression.

关键词： Erythrocyte miRNA MGEA5 miR-144 miR-10b Nile tilapia

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Fish red blood cells express immune genes and responses

引用

Aquaculture and Fisheries 2018年第1期3卷 14-21页

作者： yawei shen Dan Wang Jinliang Zhao Xiaowu Chen National Demonstration Center for Experimental Fisheries Science Education Shanghai Ocean UniversityShanghai 201306China Shanghai Engineering Research Center of Aquaculture Shanghai Ocean UniversityShanghai 201306China Shanghai Collaborative Innovation for Aquatic Animal Genetics and Breeding Shanghai Ocean UniversityShanghai 201306China

The erythrocytes or red blood cells(RBCs)and leucocytes or white blood cells(WBCs)are the most abundant cell types in the circulatory system of *** RBCs are responsible for gas exchange while WBCs are solely involved with *** this study,we present evidence that fish RBCs despite their respiratory role they also express immune-related genes and responses in the teleost fish,Nile tilapia(Oreochromis niloticus).Fluorescent staining identified the presence of numerous mitochondria and lysosomes in tilapia *** sequencing of tilapia RBCs and WBCs led to the assembly and annotation of 20.876 transcripts of which 1017 and 3628 transcripts were specific to RBCs and WBCs,*** sequence comparisons identified 3251 transcripts that were significantly differential expressed of which 707(21.75%)were up-regulated and 2544(78.25%)down-regulated in RBCs when compared to WBCs.809 transcripts were assigned to 16 immune system pathways,21 and 163 transcripts were specifically expressed in tilapia RBCs and WBCs respectively,and 23 and 233 transcripts were highly expressed in RBCs and WBCs *** of 7 immune genes(interferon regulatory factors(irf)1e5 and 9 and tlr3)in both WBCs and RBCs was validated by RT-PCR *** real-time PCR revealed that all genes were up-regulated in poly(I:C)-challenged WBCs and irf(1,3,4,and 9)and tlr3 were also significantly up-regulated in poly(I:C)-challenged ***,fish RBCs expressed immunity genes and responses and they may play a complementary role in vertebrate immunity.

关键词： Red blood cell Toll-like receptor Transcriptome White blood cell

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Hemocytes transcriptome profile of the Chinese mitten crab(Eriocheir sinensis)

引用

Aquaculture and Fisheries 2018年第5期3卷 191-195页

作者： Xiaowu Chen yawei shen Yanwui Bi Xugan Wu National Demonstration Center for Experimental Fisheries Science Education Shanghai Ocean UniversityShanghai 201306China Shanghai Engineering Research Center of Aquaculture Shanghai Ocean UniversityShanghai 201306China Shanghai Collaborative Innovation for Aquatic Animal Genetics and Breeding Shanghai 201306China

The Chinese mitten crab(Eriocheir sinensis)possesses an open circulatory system,in which hemolymph moves through interconnected sinuses or spaces surrounding organs called *** hemocytes are classified into hyalinocytes,semigranulocytes,and granulocytes;and limited transcriptomics research is *** this study,the transcriptome of the crab *** hemocytes was characterized.A total of 14,380,229 clean reads representing a total of 4.31 Gb nucleotides dataset were produced.A total of 67,047 contigs were obtained and 12.49%(8375)and 9.74%(6533)of the contigs were matched to data publicly available from the GenBank nr nucleotide and Uniprot databases,*** these contigs,4344 contigs belong to three categories of Gene Ontology,126 contigs to 21 subcategories of KEGG,and 4962 contigs to 25 categories of the COG database.A total of 508,379 and 345 transcripts of the *** showed>40%sequence similarity with transcripts expressed in the vertebrate blood cells from tilapia(Oreochromis niloticus),Chinese softshell turtle(Trionyx sinensis)and chicken(Gallus gallus)respectively.A total of 53,077 single nucleotide polymorphisms were identified and 9912 SSRs for microsatellite mining were *** most frequent repeat motifs detected were dinucleotide repeats,accounting for 37.52%of the total *** data provides an important gene resource for the exploitation of molecular marker-assisted breeding,immune mechanisms and conservation of germplasm in the crab ***.

关键词： Eriocheir sinensis Hemocytes Transcriptome

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Prostaglandin E_(2) involvement in the reproduction of small abalone,Haliotis diversicolor

引用

Aquaculture and Fisheries 2024年第5期9卷 804-811页

作者： yawei shen Lingling Li Xuan Luo Miaoqin Huang Caihuan Ke Weiwei You Weidong Li State Key Laboratory of Marine Environmental Science College of Ocean and Earth SciencesXiamen UniversityXiamen361102China Fujian Key Laboratory of Genetics and Breeding of Marine Organisms Xiamen UniversityXiamen361102China School of Life Sciences Xiamen UniversityXiamen361102China College of Ecology and Environment Hainan UniversityHaikou570228China

Prostaglandin E2(PGE2)has been reported to be involved in reproduction as one of the most abundant and widely distributed prostaglandins in *** gonad development and gamete production in mollusks can greatly assist artificial breeding,which is believed to promote the commercial benefits of various mollusk *** the present study,the contents of PGE2 in the gonads of abalone at different developmental stages(maturing stage,ripe stage,and spawning stage)and reproduction states(before and after spawning)were measured.A higher PGE2 level was found in the gonads of abalones at the ripe stage than other stages,and the highest PGE2 level was detected in the ovaries of female *** in the UV-stimulated *** the effects of PGE2 injection on the spawning efficiency were explored,and the gamete emission rates of female and male abalone were significantly increased after PGE2 *** qRT-PCR and in situ hybridization assay,higher expression levels and wide distribution of phospholipase A2(PLA2),cyclooxygenase 2(COX2),prostaglandin E synthases(PGES2),and prostaglandin E2 receptor 4(EP4)in the gonads of *** were observed after *** is speculated that PGE2 may promote the reproductive cycle in both male and female ***,although the regulation mechanism of PGE2 in female and male abalone might be *** results will be useful for the application of PGE2 in the abalone aquaculture practices to increase the reproduction efficiency in future.

关键词： Prostaglandin Abalone Reproduction Gonad

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Transcriptome analysis of gill from Lateolabrax maculatus and aqp3 gene expression

引用

Aquaculture and Fisheries 2019年第6期4卷 247-254页

作者： yawei shen Yudong He Yanhui Bi Jianpeng Chen Ziwei Zhao Jun Li Xiaowu Chen National Demonstration Center for Experimental Fisheries Science Education Shanghai Ocean UniversityShanghai201306China Shanghai Engineering Research Center of Aquaculture Shanghai201306China Shanghai Collaborative Innovation for Aquatic Animal Genetics and Breeding Shanghai201306China CAS Key Laboratory of Experimental Marine Biology Institute of OceanologyChinese Academy of SciencesQingdao26000China Laboratory for Marine Biology and Biotechnology Qingdao National Laboratory for Marine Science and TechnologyQingdao26000China

The Lateolabrax maculatus is an important marine fish in China that can adapt to a wide range of water *** gill is the main organ involved in fish osmoregulation and in this study,a cDNA library of the *** gill was constructed to understand gill function in Lateolabrax maculatus and it adaptation to salinity.A total raw data of 5.3G base pairs was obtained,followed removal of low-quality and linker DNA sequences and it yielded a total of 23,038,590 clean *** splicing and clustering,31,976 unigenes were *** average transcript length was 1327 bp and N50 was 2057 *** comparisons with other tissues analysis of the gill transcriptome revealed a high level of relative expression of apolipoprotein E,CD74,keratin,type I cytoskeleton 13,carbonic anhydrase,betaine-homocysteine S-methyltransferase,and aquaporin 3(AQP3)*** *** aqp3(Lm-aqp3)gene was specific for the gills Lm-aqp3 and this gene has a typical transmembrane structure of six alpha helices and a water channel *** analysis indicated that Lm-aqp3 clusters with the homologue genes from Labrus bergylta and Dicentrarchus *** RT-PCR confirmed that Lm-aqp3 was highly abundant in the gills and low expressed in other tissues(stomach,liver,brain,spleen and testis).Analysis of Lm-aqp3 in freshwater aquaculture fish revealed that gene expression was significantly statistical higher than in seawater(20 ppt)fish,suggesting that Lm-aqp3 may play an important role in water metabolism and osmotic pressure regulation in ***.

关键词： Lateolabrax maculatus Gill Transcriptome Aquaporin 3

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

基于鲁棒反馈控制理论的路径跟踪控制器设计

引用

汽车技术 2023年第7期 47-53页

作者：沈亚伟赵又群南京航空航天大学南京210016

为了提高智能车辆路径跟踪的精确性和鲁棒性,基于李雅普诺夫稳定性(Lyapunov Stability)理论设计了鲁棒反馈路径跟踪控制器,运用舒尔(Schur)补引理并求解线性矩阵不等式(LMI)得到控制系统的反馈矩阵。通过搭建CarSim/Simulink联合仿真... 详细信息

为了提高智能车辆路径跟踪的精确性和鲁棒性,基于李雅普诺夫稳定性(Lyapunov Stability)理论设计了鲁棒反馈路径跟踪控制器,运用舒尔(Schur)补引理并求解线性矩阵不等式(LMI)得到控制系统的反馈矩阵。通过搭建CarSim/Simulink联合仿真平台将鲁棒反馈控制器与线性二次型调节器(LQR)进行对比分析,以不同的车速分别在不同附着系数的路面上进行仿真,验证所设计控制器的性能。仿真结果表明:在不同路面和车速工况下,相较于LQR控制器,基于李雅普诺夫稳定性理论的鲁棒反馈控制器具有更高的控制精度,同时具有更强的鲁棒性。

关键词：智能车辆路径跟踪线性矩阵不等式鲁棒控制李雅普诺夫稳定性

来源：

维普期刊数据库

同方期刊数据库博看期刊评论

在线全文

学校读者我要写书评

暂无评论

地铁施工对邻近桥桩变形和受力影响研究

引用

现代城市轨道交通 2019年第10期 32-37页

作者：沈亚威南京地铁建设有限责任公司

以南京地铁11号线下穿南京长江大桥北引桥为工程背景,利用数值模拟软件研究地铁施工过程中临近桥桩的变形和受力变化规律。研究结果表明,当桥桩底标高和隧道底标高平齐时,地铁施工会使得桥桩产生竖向刚体位移1.15m m,在与隧道轴线平齐... 详细信息

以南京地铁11号线下穿南京长江大桥北引桥为工程背景,利用数值模拟软件研究地铁施工过程中临近桥桩的变形和受力变化规律。研究结果表明,当桥桩底标高和隧道底标高平齐时,地铁施工会使得桥桩产生竖向刚体位移1.15m m,在与隧道轴线平齐处桥桩侧向位移最大达到2.06mm;桥桩中部竖向正应力最大达到0.64MPa,相比开挖前增长了276%;隧道开挖扰动影响桥桩变形和受力的范围为桥桩距隧道掌子面水平距离前后20m。为确保桥桩的稳定,需采取相应的加固措施减小隧道开挖扰动的影响。

关键词：地铁隧道桥桩变形受力数值模拟

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

术前中性粒细胞/淋巴细胞比值、血小板/淋巴细胞比值对UAE患者术后疼痛的预测价值

引用

介入放射学杂志 2023年第10期32卷 992-995页

作者：沈亚伟刘凯吕军郑州大学第五附属医院介入科河南郑州450052

目的探讨术前中性粒细胞/淋巴细胞比值(neutrophil to lymphocyte ratio,NLR)、淋巴细胞/单核细胞比值(lymphocyte to monocyte ratio,LMR)、血小板/淋巴细胞比值(platelet to lymphocyte ratio,PLR)与子宫动脉栓塞术(uterine artery embolization,UAE)后疼痛的关系。方法回顾性分析2020年7月至2022年1月郑州大学第五附属医院193例接受UAE治疗的子宫肌瘤患者的临床资料,计算术前NLR、PLR、LMR值。采用数值评分法(numerical rating scale,NRS)评估患者术后1、6、24、48、72 h疼痛程度。Pearson分析NLR、LMR、PLR与术后疼痛的相关性。用ROC曲线分析NLR、PLR的AUC值并确定最佳截断值,将PLR、NLR组合成高NLR+高PLR组、高NLR+低PLR组、低NLR+高PLR组、低NLR+低PLR组,对比各组术后疼痛的差异。结果术前NLR(R=0.217,P<0.05)、PLR(R=0.475,P<0.05)均与UAE术后疼痛程度呈明显正相关。以NLR=1.78,PLR=166.91为界值,高NLR组和高PLR组患者的住院时间、各时间点NRS评分、镇痛药摄入量均大于低NLR和低PLR组(P<0.05)。与低NLR+低PLR组相比,高NLR+高PLR组术后1、6、24、48、72 h NRS评分及镇痛药摄入量均显著升高(P<0.05)。结论NLR、PLR对子宫肌瘤UAE术后疼痛有较高的评估价值,联合指标评估术后疼痛的作用优于单独指标。

关键词：中性粒细胞/淋巴细胞比值淋巴细胞/单核细胞比值血小板/淋巴细胞比值子宫动脉栓塞术疼痛

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

矿山法地铁隧道下穿既有铁路路基影响研究

引用

现代城市轨道交通 2019年第7期 67-71页

作者：沈亚威南京地铁建设有限责任公司

南京地铁4号线桦墅站—仙林东站区间隧道需下穿铁路专用线路基,区间隧道顶部与铁路路基轨道最小净距仅6.2m。以此工程为背景,采用三维数值模拟方法对矿山法隧道施工引起的地表路基整体沉降及路基不均匀沉降进行分析,并提出相应的保护措施。

关键词：地铁隧道下穿铁路近接施工路基沉降

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：