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Outcomes of the study of intracytoplasmic sperm injection(ICSI)and sperm motility with microdissection testicular sperm extraction

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Asian Journal of Andrology 2022年第2期24卷 221-222页

作者： Yuuka Arai Hiroe Ueno Mizuki Yamamoto Haruna Izumi Kazumi Takeshima Tomonari Hayama Hideya Sakakibara yasushi yumura Etsuko Miyagi Mariko Murase Reproductive Medical Center Yokohama City University Medical CenterYokohama-shi 232-0024Japan Department of Gynecology Yokohama City University Medical CenterYokohama-shi 232-0024Japan Department of Obstetrics and Gynecology Yokohama City University School of MedicineYokohama-shi 236-0004Japan

Dear Editor,Microdissection testicular sperm extraction(micro-TESE)is an optimal sperm extraction method for men with nonobstructive azoospermia(NOA).1 According to a recent systematic review by Corona et al.,2 the sperm retrieval rate by micro-TESE and live birth rate per intracytoplasmic sperm injection(ICSI)cycle among NOA patients were 46%and 24%,respectively.In cases where sperm can be collected from NOA patients,the live birth rate remains low.If the potential outcomes of ICSI can be predicted by sperm motility assessment,such as pregnancy not anticipated because of immotile sperm,unnecessary ovarian stimulation or oocyte retrieval from female partners could be avoided.

关键词： motility injection stimulation

Inverse correlation between reactive oxygen species in unwashed semen and sperm motion parameters as measured by a computer-assisted semen analyzer

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Asian Journal of Andrology 2017年第3期19卷 350-354页

作者： Teppei Takeshima yasushi yumura Kengo Yasuda Hiroyuki Sanjo Shinnosuke Kuroda Hiroyuki Yamanaka Akira Iwasaki Department of Urology Reproductive Center Yokohama City University Medical Center Yokohama Kanagawa Japan YMRAC Urology Office Yokohama Kanagawa Japan.

This study investigated the correlation between sperm motion parameters obtained by a computer-assisted semen analyzer and levels of reactive oxygen species in unwashed semen. In total, 847 patients, except for azoospermic patients were investigated. At the time of each patient＇s first consultation, semen parameters were measured using SMASTM or CellSoft 3000TM, and production of reactive oxygen species was measured using a computer-driven LKB Wallac Luminometer 1251 Analyzer. The patients were divided into two groups： reactive oxygen species - positive and negative. The semen parameters within each group were measured using one of the two computer-assisted semen analyzer systems and then compared. Correlations between reactive oxygen species levels and sperm motion parameters in semen from the reactive oxygen species - positive group were also investigated. Reactive oxygen species were detected in semen samples of 282 cases （33.3%）. Sperm concentration （P 〈 0.01; P 〈 0.01）, motility （P 〈 0.01; P 〈 0.05）, and progressive motility （P 〈 0.01; P 〈 0.01） were markedly lower in the reactive oxygen species - positive group than in the reactive oxygen species - negative group. Among the sperm motion parameters in the reactive oxygen species - positive group, sperm concentration （P 〈 0.01; P 〈 0.01）, motility （P 〈 0.05; P 〈 0.01）, mALH （P 〈 0.05; P 〈 0.01）, and progressive motility （P 〈 0.05; P 〈 0.01） also showed inverse correlations with the logarithmic transformed reactive oxygen species levels. Therefore, this study demonstrated that excessive reactive oxygen species in semen damage sperm concentration, motility, and other sperm motion parameters.

关键词： oxidative stress reactive oxygen species semen analysis sperm motility

Current status of sperm banking for young cancer patients in Japanese nationwide survey

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Asian Journal of Andrology 2018年第4期20卷 336-341页

作者： yasushi yumura Akira Tsujimura Hiroshi Okada Kuniaki Ota Masahumi Kitazawa Tatsuya Suzuki Tosiyuki Kakinuma Seido Takae Nao Suzuki Teruaki Iwamoto Reproduction Center Yokohama City University Medical Center Yokohama 232-0024 Japan Department of Urology Juntendo University Urayasu Hospital Chiba 279-0021 Japan Department of Urology Dokkyo Medical University Koshigaya Hospital Saitama 343-8555 Japan Department of Obstetrics and Gynecology Nasu Red Cross Hospital Tochigi 324-8686 Japan Department of Obstetrics and Gynecology Dokkyo Medical University Tochigi 321-0293 Japan Department of Obstetrics and Gynecology Jichi Medical University Tochigi 329-0498 Japan Department of Obstetrics and Gynecology International University of Health and Welfare Tochigi 329-2763 Japan Department of Obstetrics and Gynecology St. Marianna University School of Medicine Kawasaki 216-8511 Japan Division Male Infertility Center for Human Reproduction International University of Health and Welfare Sanno Hospital Tokyo 107-0052 Japan

This study aimed to ascertain the current status of Japanese sperm banking for young cancer patients. During 2015, we mailed the directors of 695 institutes where sperm cryopreservation might be performed with questionnaires requesting information on the number of patients, age, precryopreservation chemotherapy, semen analyses results and diagnoses, cryopreservation success rate, and causes of unsuccessful cryopreservation. Of these 695 institutes, 92 had cryopreserved sperm before chemotherapy within the study period. In all, 820 cancer patients （237 testicular, 383 hematological, 46 bone and soft tissue, 20 brain, and 134 other malignancy） consulted the responding institutes for sperm cryopreservation. Except for testicular tumor, the number of patients whose sperm was preserved before cancer treatment was low compared to that of young cancer patients. Approximately 20% of patients with malignancies other than testicular tumor underwent chemotherapy before cryopreservation. The success rate of cryopreservation in hematological malignancy was 82.5%, significantly lower than that of both the testicular cancer （93.6%） and other malignancy groups （95.6%）（P 〈 0.05）. The primary reasons for preservation failure were azoospermia and poor semen quality. Patients with hematological malignancies had a higher rate of unsuccessful cryopreservation compared to those in other groups, possibly due to the large number of patients requesting sperm cryopreservation after chemotherapy induction. In Japan, information regarding sperm banking prior to cancer treatment appears to be lacking. Information regarding sperm preservation before chemotherapy should be provided to all Japanese oncologists.

关键词： cancer chemotherapy fertility preservation sperm preservation survey

Certified Infertility Nurses’ Perceptions and Practice on Male Infertility Nursing and Related Factors

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Open Journal of Nursing 2018年第1期8卷 1-13页

作者： Akiko Mori Kyoko Asazawa Ruriko Hoshi yasushi yumura Department of Midwifery and Women’s Health Graduate School of Nursing Science St. Luke’s International University Tokyo Japan Department of Nursing Tokyo Healthcare University Tokyo Japan Nursing Department Yokohama City University Medical Center Yokohama Japan Reproduction Center Yokohama City University Medical Center Yokohama Japan

Purpose: The aims of this study were to clarify the complaints and concerns expressed by couples that included a partner with male infertility, certified infertility nurses’ nursing practice and perceptions of nursing for these couples, and factors related to these perceptions and practices. Methods: A quantitative cross-sectional study was conducted. Prospective participants were sent an anonymous self-administered questionnaire containing items pertaining to the complaints and concerns expressed by couples that included a partner with male infertility, and nursing practice and perceptions of nursing for these couples. Results: In total, 133 questionnaires were distributed, 74 responses were received, and 69 of these responses were valid. Couples’ complaints and concerns consisted of a three-factor structure comprising psychological burden, lack of knowledge and information, and problems with partners. Nurses reported that the complaints and concerns of couples that included a partner with male infertility differed between male and female partners. Factors related to nursing practice and nurses’ perceptions of nursing were identified. Conclusions: Participants felt that psychological burden and problems were more serious for female partners than they were for male partners, and concern regarding the physical health of the partner receiving treatment was greater in male partners than it was in female partners.

关键词： Professional Practice Fertility Nurse Male Infertility

Energetics of dioxygen binding into graphene patches with various sizes and shapes

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Science China Chemistry 2012年第5期55卷 787-795页

作者： yumura Takashi KOBAYASHI Hisayoshi YAMABE Tokio Department of Chemistry and Materials Technology Kyoto Institute of Technology Matsugasaki Sakyo-ku Kyoto 606-8585 Japan Nagasaki Institute of Applied Science 536 Aba-machi Nagasaki 851-0193 Japan

Density functional theory (DFT) calculations were employed to investigate the electronic properties of an H-atom terminated graphene patch (hydrographene) smaller than a rhombic C96H26 structure with zigzag edges. Depending on shapes and sizes of hydrographenes, some hydrographenes have the triplet ground state where unpaired electrons are localized on their zigzag edges. The stability of the triplet spin state is diminished, decreasing the hydrographene sizes. The existence of the localized spin densities allows triplet dioxgen to bind into a hydrographene. According to the DFT calculations, the energetics of the dioxygen bindings is negatively influenced by downsizing hydrographenes, as well as depends on their shapes. The size-and shape-dependences of the dioxygen bindings reflect from the stability of the triplet state of a hydrographene, because its localized unpaired electrons can be utilized to be attached to an unpaired electron of triplet dioxygen.

关键词：形状补丁石墨尺寸能量 DFT计算氧电子性质