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BRIEF ARTICLE New reduced volume preparation regimen in colon capsule endoscopy

World Journal of Gastroenterology 2012年第17期18卷 2092-2098页

作者： yasuo Kakugawa Yutaka Saito Shoichi Saito Kenji Watanabe Naoki Ohmiya Mitsuyuki Murano Shiro Oka Tetsuo Arakawa Hidemi Goto Kazuhide Higuchi Shinji Tanaka Hideki Ishikawa Hisao Tajiri Division of Screening Technology and Development Research Center for Cancer Prevention and ScreeningNational Cancer CenterTokyo 104-0045Japan Endoscopy Division National Cancer Center HospitalTokyo 104-0045Japan Department of Endoscopy Jikei University School of MedicineTokyo 105-8461Japan Department of Gastroenterology Osaka City University Graduate School of MedicineOsaka 545-8685Japan Department of Therapeutic Medicine Nagoya University Graduate School of MedicineNagoya 466-8560Japan Second Department of Internal Medicine Osaka Medical CollegeOsaka 569-8686Japan Department of Endoscopy Hiroshima University HospitalHiroshima 734-8551Japan Department of Molecular-Targeting Cancer Prevention Graduate School of Medical ScienceKyoto Prefectural University of MedicineKyoto 602-0841Japan

AIM:To evaluate the effectiveness of our proposed bowel preparation method for colon capsule ***:A pilot,multicenter,randomized controlled trial compared our proposed "reduced volume method"(group A) with the "conventional volume method"(group B) preparation *** A did not drink polyethylene glycol electrolyte lavage solution(PEGELS) the day before the capsule procedure,while group B drank 2 *** the procedure day,groups A and B drank 2 L and 1 L of PEG-ELS,respectively,and swallowed the colon capsule(PillCam COLON capsule).Two hours later the first booster of 100 g magnesium citrate mixed with 900 mL water was administered to both groups,and the second booster was administered six hours post capsule ingestion as long as the capsule had not been excreted by that *** videos were reviewed for grading of cleansing level,RESULTS:Sixty-four subjects were enrolled,with results from 60 *** A and B included 31 and 29 subjects,***-nine(94%) subjects in group A and 25(86%) subjects in group B had adequate bowel preparation(ns).Twenty-two(71%) of the 31 subjects in group A excreted the capsule within its battery life compared to 16(55%) of the 29 subjects in group B(ns).Of the remaining 22 subjects whose capsules were not excreted within the battery life,all of the capsules reached the left side colon before they stopped functioning.A single adverse event was reported in one subject who had mild symptoms of nausea and vomiting one hour after starting to drink PEG-ELS,due to ingesting the PEG-ELS faster than ***:Our proposed reduced volume bowel preparation method for colon capsule without PEG-ELS during the days before the procedure was as effective as the conventional volume method.

关键词：胶囊结肠内镜检查随机对照试验制备方法电池寿命编制方案

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Inverse Source Locating Method Based on Graphical Analysis of Dye Plume Images in a Turbulent Flow

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Open Journal of Fluid Dynamics 2016年第4期6卷 343-360页

作者： Qianqian Shao Daichi Sekine Takahiro Tsukahara yasuo Kawaguchi Department of Mechanical Engineering Tokyo University of Science Chiba Japan

The inverse estimation of a source location of pollutant released into a turbulent flow is a probability problem instead of a deterministic one, as the turbulent flow is chaotic and irreversible. However, researches can be conducted to provide helpful instructions to the possible source location with corresponding uncertainty. This study aims to propose a method of inverse estimation of a passive-scalar source location. Experimental investigation of the dye plume characteristics released into a fully-developed turbulent flow is performed in a water channel. A planar laser-induced fluorescence (PLIF) technique is used to obtain two-dimensional images of spreading dye plumes at a bulk Reynolds number of 20,000. The distributions of high concentration areas in the PLIF images are chosen as features that characterize the traveling (diffusion) distance or time from the dye source. Graphical analysis is used to extract these high concentration areas. The procedure of graphical analysis has three steps: 1) binarization using a threshold to extract high concentration dye patches;2) labeling individual high-concentration dye patches in the binarized images;and 3) pixel-counting to measure the area and perimeter of each dye patch. We examine the variations of fractal dimension of patches, and the fractal dimension is observed to be almost constant irrespective of the distance from the source. The kurtosis of the probability density function curve of the logarithm dimensionless dye patch areas is found to be related with the downstream diffusion distance, based on which an inverse estimation method to locate a passive-scalar point source is proposed and evaluated.

关键词： Graphical Analysis Inverse Problem Passive Scalar Diffusion Source Estimation Turbulent Flow

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汞在微生物中的跨膜运输机制研究进展

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微生物学报 2014年第10期54卷 1109-1115页

作者：杜红霞 yasuo Igarashi 王定勇西南大学生物能源与环境修复研究中心重庆400715 西南大学资源环境学院重庆400715

甲基汞是一种强亲脂性、高神经毒性的有机汞化合物,可以通过生物富集或生物放大造成人类甲基汞暴露。环境中甲基汞的产生主要是厌氧微生物所调控的无机汞的甲基化。主流观点认为厌氧微生物对汞的甲基化是一种细胞内反应,因此,甲基汞的... 详细信息

甲基汞是一种强亲脂性、高神经毒性的有机汞化合物,可以通过生物富集或生物放大造成人类甲基汞暴露。环境中甲基汞的产生主要是厌氧微生物所调控的无机汞的甲基化。主流观点认为厌氧微生物对汞的甲基化是一种细胞内反应,因此,甲基汞的产生速率不仅与环境中具有汞甲基化能力的厌氧微生物的存在与活性相关,同时也与无机汞在微生物细胞中的跨膜运输过程有着重要联系。要明确无机汞经微生物甲基化的机制,就必须了解无机汞被微生物细胞生物吸收的过程,即无机汞在微生物中的跨膜运输路径。目前研究认为该过程主要有Mer抗汞操纵子转运体系、被动扩散、促进扩散和主动运输4种路径。本综述主要围绕无机汞被微生物细胞生物吸收的这4种路径展开,将系统介绍科学界对这4种路径的最新研究进展,并对相关研究进行展望,指出无机汞经促进扩散或主动运输进入到微生物细胞内将是未来研究的重点。

关键词：汞微生物甲基化跨膜运输

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Diffusion Process of High Concentration Spikes in a Quasi-Homogeneous Turbulent Flow

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Open Journal of Fluid Dynamics 2016年第4期6卷 371-390页

作者： Masaya Endo Qianqian Shao Takahiro Tsukahara yasuo Kawaguchi Department of Mechanical Engineering Tokyo University of Science Chiba Japan

When a mass spreads in a turbulent flow, areas with obviously high concentration of the mass compared with surrounding areas are formed by organized structures of turbulence. In this study, we extract the high concentration areas and investigate their diffusion process. For this purpose, a combination of Planar Laser Induced Fluorescence (PLIF) and Particle Image Velocimetry (PIV) techniques was employed to obtain simultaneously the two fields of the concentration of injected dye and of the velocity in a water turbulent channel flow. With focusing on a quasi-homogeneous turbulence in the channel central region, a series of PLIF and PIV images were acquired at several different downstream positions. We applied a conditional sampling technique to the PLIF images to extract the high concentration areas, or spikes, and calculated the conditional-averaged statistics of the extracted areas such as length scale, mean concentration, and turbulent diffusion coefficient. We found that the averaged length scale was constant with downstream distance from the diffusion source and was smaller than integral scale of the turbulent eddies. The spanwise distribution of the mean concentration was basically Gaussian, and the spanwise width of the spikes increased linearly with downstream distance from the diffusion source. Moreover, the turbulent diffusion coefficient was found to increase in proportion to the spanwise distance from the source. These results reveal aspects different from those of regular mass diffusion and let us conclude that the diffusion process of the spikes differs from that of regular mass diffusion.

关键词： Turbulent Transport High Concentration Spikes Quasi-Homogeneous Turbulent Flow Conditional Sampling Technique PIV and PLIF Measurements Passive Scalar Diffusion

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Analysis of High Concentration Spikes Appearing in Mass Plume in Nearly Homogeneous Turbulent Flow Based on the PDF Transport Equation

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Open Journal of Fluid Dynamics 2016年第4期6卷 472-495页

作者： Masaya Endo Qianqian Shao Takahiro Tsukahara yasuo Kawaguchi Department of Mechanical Engineering Tokyo University of Science Chiba Japan

To evaluate the pollutant dispersion in background turbulent flows, most researches focus on statistical variation of concentrations or its fluctuations. However, those time-averaged quantities may be insufficient for risk assessment, because there emerge many high-intensity pollutant areas in the instantaneous concentration field. In this study, we tried to estimate the frequency of appearance of the high concentration areas in a turbulent flow based on the Probability Density Function (PDF) of concentration. The high concentration area was recognized by two conditions based on the concentration and the concentration gradient values. We considered that the estimation equation for the frequency of appearance of the recognized areas consisted of two terms based on each condition. In order to represent the two terms with physical quantities of velocity and concentration fields, simultaneous PIV (Particle Image Velocimetry) and PLIF (Planar Laser-Induced Fluorescence) measurement and PLIF time-serial measurement were performed in a quasi-homogeneous turbulent flow. According to the experimental results, one of the terms, related to the condition of the concentration, was found to be represented by the concentration PDF, while the other term, by the streamwise mean velocity and the integral length scale of the turbulent flow. Based on the results, we developed an estimation equation including the concentration PDF and the flow features of mean velocity and integral scale of turbulence. In the area where the concentration PDF was a Gaussian one, the difference between the frequencies of appearance estimated by the equation and calculated from the experimental data was within 25%, which showed good accuracy of our proposed estimation equation. Therefore, our proposed equation is feasible for estimating the frequency of appearance of high concentration areas in a limited area in turbulent mass diffusion.

关键词： High Concentration Spikes Quasi-Homogeneous Turbulence Turbulent Mass Diffusion Concentration PDF Transport Equation PIV and PLIF Simultaneous Measurement

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Field-deployable rapid multiple biosensing system for detection of chemical and biological warfare agents

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Microsystems & Nanoengineering 2018年第1期4卷 498-508页

作者： Masato Saito Natsuko Uchida Shunsuke Furutani Mizuho Murahashi Wilfred Espulgar Naoki Nagatani Hidenori Nagai Yuki Inoue Tomohiko Ikeuchi Satoshi Kondo Hirotaka Uzawa yasuo Seto Eiichi Tamiya Department of Applied Physics Graduate School of EngineeringOsaka University2-1YamadaokaSuitaOsaka 565-0871Japan Advanced Photonics and Biosensing Open Innovation Laboratory(PhotoBIO-OIL) National Institute of Advanced Industrial Science and Technology(AIST)Photonics Center Osaka University P3 Bldg2-1 Yamada-OkaSuitaOsaka 565-0871Japan Nanomaterials Research Institute National Institute of Advanced Industrial Science and Technology(AIST)1-1-1 HigashiTsukuba 305-8565Japan Health Research Institute National Institute of Advanced Industrial Science and Technology(AIST)1-8-31 MidorigaokaIkedaOsaka 563-8577Japan Department of Applied Chemistry Graduate School of EngineeringOkayama University of Science1-1Ridai-choKita-kuOkayama-shi 700-0005Japan National Research Institute of Police Science 6-3-1 KashiwanohaKashiwaChiba 277-0882Japan

The threat of biological and chemical terror acts remains a growing worldwide *** is therefore a need to develop appropriate technology for the detection of chemical and biological warfare agents,with early identification intended for use by first ***,we disclose the developed autonomous air sampling and detection system for evaluation of the presence of chemical and biological warfare agents that can be harmful to the *** current device utilizes the designed mist generator-assisted air collection system(338 l min^(−1))and biosensing chip technologies,such as electrochemical measurement,Au nanoparticle-based localized surface plasmon resonance,and rapid microfluidic chip PCR for detection of minute concentrations lower than the mean lethal dose(LD_(50))of nerve gases(sarin and VX),toxic proteins(BTX/A/Hc and ricin),and pathogens(anthrax simulant).An operation time of only 5–15 min is needed for the collection and detection;sample preparation is already integrated into the system without the need for direct human *** addition to the system’s sensitivity and ease of use,its portability makes it highly beneficial for first responders,which could aid in immediate risk assessment and mitigation of on-site events.

关键词： biological and chemical agents biosensing electrochemical detection LSPR PCR rapid detection

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Separation and Purification of GST-glycerol-3-phosphate Dehydrogenase

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Agricultural Biotechnology 2016年第5期5卷 44-45页

作者： Hongmei ZHAO Shihai LI yasuo WATANABE College of Animal Sciences Yangtze University Agriculture Department Ehime University

In order to investigate the expression of glycerol-3 -phosphate dehydrogenase by GCY1 gene in recombinant Saccharomyces cerevisiae, induction culture of the S. cerevisiaestrain was performed with SD-URA 2% galactose, 3 × YP ＋ 6% glucose, SC-URA 2% galactose, and SC-URA 2% galactose ＋ 5% NaCI glyeerol-3-phosphate dehydregenase, the cultured S. cerevisiaewas comminuted followed by full-automatic high-speed purification, and SDS-PAGE gel electrophoresis was performed for molecular weight of the GST fusion protein. The results showed that after shaking culture of the S. cerevisiae containing GCY1 at 25 ℃, the OD values of its 3 × YP ＋ 6% glucose culture and SC-URA 2% galaetose ＋ 5% NaC1 culture were 8.75 and 7.35, respectively. It was shown by purification with a Profinia low-pressure liquid chromatograph that only the S. cerevisiae cultured in SC-URA 2% galactose ＋ 5% NaC1 medium expressed glycerel-3-phosphate de- hydrogenase, the molecular weight of which was detected as 65 ku by SDS-PAGE gel electrophoresis.

关键词： Saccharomyces cerevisiae Glycerol-3-phosphate dehydrogenase Galactose SDS-PAGE gel electrophoresis Separation and purification

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Angle-Resolved and Resonant Photoemission Study of the Valence Bands of α-La(0001) on W(110)

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World Journal of Condensed Matter Physics 2016年第1期6卷 17-26页

作者： Yoshiharu Enta Osamu Morimoto Hiroo Kato yasuo Sakisaka Graduate School of Science and Technology Hirosaki University Hirosaki Japan Spring-8 Service Co. Ltd. Tatsuno Japan

We report a photoelectron spectroscopic study of the valence bands of double hexagonal-close-packed (dhcp) α-La(0001) films epitaxially grown on W(110) at room temperature. The La 5d photoemission cross section in the photon energy region from 20 eV to 130 eV was obtained and the valence-band structure of α-La was determined. Except for 4f-related structures, the valence-band structures of dhcp α-La and dhcp β-Ce were found to resemble each other. From the band structure, the crystal structure of the La film was confirmed. No evidence for the existence of a 5d-like surface state near the Fermi energy at the point of the surface Brillouin zone was obtained and a 6s band bottom was identified.

关键词： Lanthanum Thin Film Electronic Structure Valence Band Tungsten Surface Photoemission Angle-Resolved Spectroscopy

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Examination of bridge inspection data by SOM/LVQ

Examination of bridge inspection data by SOM/LVQ

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The First China-Japan-Korea Joint Symposium on Optimization of Structural and Mechanical Systems

作者： yasuo CHIKATA Satoshi HIROSE Graduate School of Kanazawa Univ.（2-40-20 Kodatsuno Kanazawa920-8667Japan） Graduate School of Kanazawa Univ.（2-40-20 Kodatsuno Kanazawa920-8667Japan）

＜正＞This paper deals with the examination of the inspection or diagnosis data of existing bridges. In the inspection or diagnosis, experts make damage evaluations. But their judgments are subjective, and then sometime the judgments include contradictions. Therefore, examination of the data is required when the inspection data is used as teacher signal for the learning process of neural networks or as base data for composing expert system. This paper shows an approach by SOM/LVQ for the re-examination of the inspection data of existing bridges.

关键词： data examination bridge inspection SOM LVQ

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DERIVATIONS OF CORRELATION AND LIQUID-SOLID CONTACT MODEL OF TRANSITION BOILING HEAT TRANSFER

DERIVATIONS OF CORRELATION AND LIQUID-SOLID CONTACT MODEL OF...

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第十三届国际核工程大会

作者： Hiroyasu Ohtake yasuo Koizumi Kogakuin University Kogakuin University

＜正＞ 1 INTRODUCTIONThe objective of the present study is to examine heat transfer characteristics of transition boiling through steady heat transfer experiments and modeling. The transition boiling heat transfer was...

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