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A Study of Plaque Adhesion during Implant Treatment of Missing Molars

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Open Journal of Stomatology 2022年第3期12卷 96-102页

作者： Yuka Hasegawa Yu Kishimoto yasunori Kanemitsu Toshiichiro Tanabe Kuniteru Nagahara Tetsuji Nakamoto Department of Maxillofacial Implant School of Dentistry Asahi University Mizuho Japan Medical and Dental Center Asahi University Mizuho Japan

Purpose: In implant treatment, the abutments and superstructures form a transmucosal area. In addition to providing appropriate cleaning instructions to patients, its material properties must be carefully considered. It is not clear how much plaque adhesion occurs at each site during treatment process, and the effect of materials on plaque adhesion. Methods: In this study, 33 patients, 13 maxillary cases and 20 mandibular cases, who had implants placed in the missing molars were evaluated for the plaque adhesion of provisional restorations made of acrylic resin and monolithic zirconia final restoration after a period of more than 4 weeks. In addition, oral hygiene instructions were thoroughly given for 12 cases at the time of staining after the provisional restorations, and re-evaluated in 4 weeks. Results: About 20% of the buccal-lingual area of the prosthetic device fabricated with acrylic resin showed plaque staining, whereas about 40% of the area of the mesial and distal. On the other hand, for the zirconia final restorations, the percentage of staining was about 5% for buccal and lingual surface and 10% for mesial and distal surface. The stained area ratio of the provisional restoration correlated with the zirconia superstructure area ratio. Even with careful oral hygiene, it became clear that the amount of plaque adhesion due to the difference in materials could not be overcome. Conclusion: The zirconia superstructure is extremely good from the viewpoint of plaque adhesion, but cases with a large stained area at the time of provisional restoration should be carefully followed up.

关键词： Superstructure Dental Plaque Yttria-Stabilized Zirconia Polymethyl Methacrylate

Synthesis of Single Wall Carbon Nanotubes by Using Arc Discharge Technique in Nitrogen Atmosphere

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Synthesis of Single Wall Carbon Nanotubes by Using Arc Disch...

Intraductal papillary neoplasm of the bile duct in liver cirrhosis with hepatocellular carcinoma

Ⅻ International Symposium on Small Particles and Inorganic Clusters

作者： yasunori Makita Shinzo Suzuki Hiromichi Kataura Yohji Achiba Department of Chemistry Tokyo Metropolitan UniversityTokyo 192-0397 Japan Department of Chemistry Tokyo Metropolitan UniversityTokyo 192-0397 Japan Department of Physics Tokyo Metropolitan UniversityTokyo 192-0397 Japan Department of Chemistry Tokyo Metropolitan University Tokyo 192-0397 Japan

＜正＞ Recently, it was found that single wall carbon nanotubes （SWNTs） were synthesized in good quality by using laser furnace technique in nitrogen atmosphere [1]. On the other hand, the arc discharge technique has been extensively used for the synthesis of SWNTs, e.g., using Ni/Y or Rh/Pt carbon composite rods in He atmosphere [2]. However, those SWNTs have lots of carbon impurities, and therefore, it has been considered that SWNTs made by arc discharge technique could not be considered as raw materials aimed for further purification procedure.

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World Journal of Gastroenterology 2011年第14期17卷 1923-1926页

作者： Jing Xu yasunori Sato Kenichi Harada Norihide Yoneda Yasuni Nakanuma Teruyuki Ueda Atsushi Kawashima Akishi Ooi Department of Human Pathology Kanazawa University Graduate School of Medicine Kanazawa 920-8640 Japan Department of Pathology Shanxi Medical University Taiyuan 030001 Shanxi Province China Internal Medicine for Digestive Organs Kanazawa University Graduate School of Medicine Kanazawa 920-8640 Japan Department of Molecular Pathology Kanazawa University Graduate School of Medicine Kanazawa 920-8640 Japan

A case of intraductal papillary neoplasm of the bile duct (IPNB) arising in a patient with hepatitis B-related liver cirrhosis with hepatocellular carcinoma (HCC) is reported. A 76-year-old man was admitted to our hospital with recurrent HCC. Laboratory data showed that levels of carcinoembryonic antigen and carbohydrate antigen 19-9 were elevated. He died of progressive hepatic failure. At autopsy,in addition to HCCs,an intraductal papillary proliferation of malignant cholangiocytes with fibrovascular cores was found in the dilated large bile ducts in the left lobe,and this papillary carcinoma was associated with an invasive mucinous carcinoma (invasive IPNB). Interestingly,extensive intraductal spread of the cholangiocarcinoma was found from the reactive bile ductular level to the interlobular bile ducts and septal bile ducts and to the large bile ducts in the left lobe. Neural cell adhesion molecule,a hepatic progenitor cell marker,was detected in IPNB cells. It seems possible in this case that hepatic progenitor cells located in reactive bile ductules in liver cirrhosis may have been responsible for the development of the cholangiocarcinoma and HCC,and that the former could have spread in the intrahepatic bile ducts and eventually formed grossly visible IPNB.

关键词： Papillary carcinoma Bile duct neoplasms Liver cirrhosis Progenitor cells Hepatocellular carcinoma Neural cell adhesion molecules

Effect of L-lysine in culture medium on nodule formation by bone marrow cells

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Journal of Biomedical Science and Engineering 2012年第10期5卷 587-592页

作者： Masataka Yoshikawa yasunori Shimomura, Hideyuki Kakigi Norimasa Tsuji Takayoshi Yabuuchi Hiroyuki Hayashi Department of Endodontics Osaka Dental University Osaka Japan

The aim of this study was to estimate the effect of L-lysine on nodule formation by rat bone marrow cells in vitro. In this study, L-lysine was added to medium for mesenchymal stem cell culture to promote proliferation and differentiation of the cells, and then nodule formation was estimated in an in vitro rat bone marrow cell culture. Bone marrow cells from the bone shafts of the femora of Fischer 344 rats were cultured in minimum essential medium with 20 μl of L-lysine solution at 10﹣4, 10﹣5, 10﹣6, 10﹣7 or 10﹣8 M. Dexamethasone was also added to the medium at 10 nM for differentiation of stem cells from bone marrow into osteoblast progenitor cells. The subculture was performed for 2 weeks. The quantity of osteocalcin in rat bone marrow cell culture with dexamethasone was 392 ng/ml. In the medium with dexamethasone and 10﹣8 M L-lysine, the quantity of osteocalcin was 437 ng/ml. Nodules only formed upon addition of 20 μl of L-lysine at 10﹣8 M. It was indicated that 10﹣8 M L-lysine should be the optimal concentration for calcification. For nodule formation by rat bone marrow cells in vitro, the optimum concentration of L-lysine in culture medium might be 20 μl of 10﹣8 M. L-lysine could play an important role in matrix production for bone formation in vitro.

关键词： L-Lysine Bone marrow Cells Calcium Deposition Dexamethasone In Vitro

Improving Flow Property of Nifedipine Loaded Solid-Lipid Nanoparticles by Means of Silica for Oral Solid Dosage Form

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Pharmacology & Pharmacy 2014年第12期5卷 1119-1129页

作者： Ranjan Kumar Barman yasunori Iwao Shuji Noguchi Mir Imam Ibne Wahed Shigeru Itai Department of Pharmaceutical Engineering and Drug Delivery Science School of Pharmaceutical Sciences University of Shizuoka Shizuoka Japan Department of Pharmacy Faculty of Science University of Rajshahi Rajshahi Bangladesh

In this study, a new formulation of silica nanocomposite containing nifedipine (NI) loaded freeze-dried solid-lipid nanoparticles (NI-SLNs) and silica have been developed with improved flowability of powders, which can lead to the formulation of a widely acceptable oral dosage form. The stable NI-SLNs were prepared using two phospholipids, hydrogenated soybean phosphatidylcholine and dipalmitoylphosphatidylglycerol mixed with 2.5% w/v trehalose as a cryoprotectant followed by lyophilization. We employed various grades of two types of silica, such as fumed and precipitated. Silica improved the poor flow property of NI-SLNs to good category as per USP-29. In addition, most of the silica nanocomposites showed the satisfactory results in their physicochemical properties such as particle size, polydispersity index, zeta potential, and recovered potency by around 100 nm, 0.3, -50 mV, and 80%, respectively. Furthermore, it was found that NI-SLNs were easily released form nanocomposites within 30 min, therefore, suggesting an improvement of drug dissolutions. Among them, precipitated silica cooperated fairly in improving the powder characteristics as well as the physicochemical, morphological, and pharmaceutical properties.

关键词： Silica Solid-Lipid Nanoparticle Solid Dosage Form Nifedipine Flowability

Experimental and Numerical Analysis of Rock Block Stability Using a Remotely Positioned Laser Doppler Vibrometer

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Journal of Civil Engineering and Architecture 2014年第5期8卷 624-632页

作者： Guichen Ma Kazuhide Sawada Atsushi Yashima Fumiaki Uehan Osamu Murata Hideki Saito yasunori Ootsuka Engineering Headquarters O YO Corporation Saitama 331-8688 Japan Department of Civil Engineering Gifu University Gifu 501-1193 Japan Structural Mechanics Laboratory Railway Technical Research Institute Tokyo 185-8540 Japan

This paper examines a new method of evaluating the stability of a rock slope using a remotely positioned LDV （laser Doppler vibrometer）. We conducted an experiment using physical models and performed a numerical analysis to evaluate the new method. The physical model included：（l） concrete blocks on an artificial soil slope with two block sizes and three slopes; （2） concrete blocks bonded to the concrete base with different contact area. The LDV measurements agreed with conventional seismometer measurements. The dominant frequency of the blocks varied with the stability and dominant frequency and the amplitude varied with the block size. The numerical model was used to examine a concrete block adhered to a concrete base with different contact areas. The dominant frequency of the blocks determined using the numerical model agreed with those obtained from the physical experiments. We analyzed different sized blocks to examine the scaling effects. The dominant frequency of the blocks was inversely related to the block size. These results demonstrated the effectiveness of LDV for evaluating the stability of rock slopes and cleared the block size scaling effects.

关键词： Field measurements risks and hazards rock failure rock mass stability analysis.

Dextran coating on and among fibers of polymer sponge scaffold for osteogenesis by bone marrow cells in vivo

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Journal of Biomedical Science and Engineering 2010年第8期3卷 751-757页

作者： Masataka Yoshikawa Norimasa Tsuji Hideyuki Kakigi Takayoshi Yabuuchi yasunori Shimomura Hiroyuki Hayashi Hajime Ohgushi 不详

Although hydroxyapatite is commonly used as a scaffold for bone regeneration, sponges may be suitable because of the adaptability to the defect. To use as a scaffold, the fiber of sponge would be coated with any adhesive to storage stem cells in the sponges. Fiber in the structure of commercially available sponges was coated by immersion in dextran solution and air dried. After seeding of rat bone marrow cells (rBMCs), the sponges were implanted subcutis of rats for estimate osteogenesis in vivo. The level of osteocalcin was 25.28 ±5.71 ng/scaffold and that of Ca was 129.20 ±19.69 μg/scaffold. These values were significantly high- er than those in sponges without dextran coating (p <0.01). It was thought that rBMCs could be stored on the shelf by dextran deposition in the fiber of the sponge. In vivo examination, dextran induced osteogenesis by rBMCs in many spaces in the inner structure of the sponge.

关键词： Dextran Cell Adhesion Scaffold Bone Marrow Cells Osteogenesis

Functional analysis of antibacterial activity of Bacillus amyloliquefaciens phage endolysin

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Functional analysis of antibacterial activity of Bacillus am...

Arsenic Polluted Groundwater and Its Countermeasures in the Middle Basin of the Ganges, Uttar Pradesh State, India

The Seventh China-Japan Joint Symposium on Enzyme Engineering

作者： yasunori Tanji Masatomo Morita Yuji Orito Katsutoshi Hori Hajime Unno Department of Bioengineering Tokyo Institute of Technology

To analyze the antibacterial activity of Bacillus amyloliquefaciens phage endolysin,nine deletion derivatives of the endolysin were *** deletion mutant was overexpressed,purified and *** catalytic domain was located on the N-terminal region and the C-terminus had an affinity with the bacterial *** enzymatic activity remained in spite of the deletion of the C-terminal 116-amino-acid region;however;the antibacterial activity was lost These results indicate that antibacterial action requires both the C-terminal cell-binding and the N-terminal enzymatic activities.

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Journal of Environmental Protection 2012年第8期3卷 856-862页

作者： yasunori Yano Kenichi Ito Akihiko Kodama Koichiro Shiomori Shigeki Tomomatsu Mitsuhiro Sezaki Hiroshi Yokota Center for International Relations University of Miyazaki Japan Faculty of Engineering University of Miyazaki Japan Retec Engineering INC Japan University of Miyazaki Miyazaki Japan

The arsenic contamination of groundwater in Uttar Pradesh State was first recognized in 2003 and is now seen at 20 Districts out of 70 Districts. University of Miyazaki has performed the arsenic mitigation project in Bahraich District, severest arsenic-affected one in the 20 Districts, from June 2008 until now, with JICA (Japan International Cooperation Agency). The integrated mitigation, such as the raising awareness of villager, installing of alternative water supply units and healthcare of arsenocosis patients, have been executed at the 2 villages. The symptom of the arsenocosis patients was not so severe, which will be, therefore, improved by drinking arsenic-safe water supplied through arsenic removal units, installed by this project. In this paper, following results is discussed for the situation and mechanism of arsenic contamination of groundwater, objected in connection with the installation of arsenic removal units: 1) Groundwater is almost contaminated with arsenic in deep tubewell (depth: about 30 m), but scarcely in shallow tubewell (depth: about 10 m);2) Arsenic contaminated groundwater is under the reduced condition with the oxidized condition for no-arsenic contaminated groundwater;3) Arsenic concentration shows almost linear correlation with concentrations of Fe2+ and -N;4) Ground is composed of sand with high arsenic content at around 25 m depth;5) Arsenic exists mainly in the phase of reducible fraction or weak acid soluble fraction but no oxidizable fraction in the ground.

关键词： Arsenic Contamination Ganges Groundwater Soil Mechanism Removal