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Effect of L-lysine in culture medium on nodule formation by bone marrow cells

Journal of Biomedical Science and Engineering 2012年第10期5卷 587-592页

作者： Masataka Yoshikawa yasunori shimomura, Hideyuki Kakigi Norimasa Tsuji Takayoshi Yabuuchi Hiroyuki Hayashi Department of Endodontics Osaka Dental University Osaka Japan

The aim of this study was to estimate the effect of L-lysine on nodule formation by rat bone marrow cells in vitro. In this study, L-lysine was added to medium for mesenchymal stem cell culture to promote proliferation and differentiation of the cells, and then nodule formation was estimated in an in vitro rat bone marrow cell culture. Bone marrow cells from the bone shafts of the femora of Fischer 344 rats were cultured in minimum essential medium with 20 μl of L-lysine solution at 10﹣4, 10﹣5, 10﹣6, 10﹣7 or 10﹣8 M. Dexamethasone was also added to the medium at 10 nM for differentiation of stem cells from bone marrow into osteoblast progenitor cells. The subculture was performed for 2 weeks. The quantity of osteocalcin in rat bone marrow cell culture with dexamethasone was 392 ng/ml. In the medium with dexamethasone and 10﹣8 M L-lysine, the quantity of osteocalcin was 437 ng/ml. Nodules only formed upon addition of 20 μl of L-lysine at 10﹣8 M. It was indicated that 10﹣8 M L-lysine should be the optimal concentration for calcification. For nodule formation by rat bone marrow cells in vitro, the optimum concentration of L-lysine in culture medium might be 20 μl of 10﹣8 M. L-lysine could play an important role in matrix production for bone formation in vitro.

关键词： L-Lysine Bone marrow Cells Calcium Deposition Dexamethasone In Vitro

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Dextran coating on and among fibers of polymer sponge scaffold for osteogenesis by bone marrow cells in vivo

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Journal of Biomedical Science and Engineering 2010年第8期3卷 751-757页

作者： Masataka Yoshikawa Norimasa Tsuji Hideyuki Kakigi Takayoshi Yabuuchi yasunori shimomura Hiroyuki Hayashi Hajime Ohgushi 不详

Although hydroxyapatite is commonly used as a scaffold for bone regeneration, sponges may be suitable because of the adaptability to the defect. To use as a scaffold, the fiber of sponge would be coated with any adhesive to storage stem cells in the sponges. Fiber in the structure of commercially available sponges was coated by immersion in dextran solution and air dried. After seeding of rat bone marrow cells (rBMCs), the sponges were implanted subcutis of rats for estimate osteogenesis in vivo. The level of osteocalcin was 25.28 ±5.71 ng/scaffold and that of Ca was 129.20 ±19.69 μg/scaffold. These values were significantly high- er than those in sponges without dextran coating (p <0.01). It was thought that rBMCs could be stored on the shelf by dextran deposition in the fiber of the sponge. In vivo examination, dextran induced osteogenesis by rBMCs in many spaces in the inner structure of the sponge.

关键词： Dextran Cell Adhesion Scaffold Bone Marrow Cells Osteogenesis

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