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A novel equation and nomogram including body weight for estimating prostate volumes in men with biopsy-proven benign prostatic hyperplasia

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Asian Journal of Andrology 2012年第5期14卷 703-707页

作者： Yasukazu Nakanishi Hitoshi Masuda Satoru Kawakami Mizuaki Sakura yasuhisa fujii Kazutaka Saito Fumitaka Koga Masaya Ito lunji Yonese Iwao Fukui Kazunori Kihara Department of Urology Tokyo Medical and Dental University Tokyo 113-8519 Japan Department of Urology Cancer institute Hospital Tokyo 135-8550 Japan

Anthropometric measurements, e.g., body weight （BW）, body mass index （BMI）, as well as serum prostate-specific antigen （PSA） and percent-free PSA （%fPSA） have been shown to have positive correlations with total prostate volume （TPV）. We developed an equation and nomogram for estimating TPV, incorporating these predictors in men with benign prostatic hyperplasia （BPH）. A total of 1852 men, including 1113 at Tokyo Medical and Dental University （TMDU） Hospital as a training set and 739 at Cancer Institute Hospital （CIH） as a validation set, with PSA levels of up to 20 ng m1-1, who underwent extended prostate biopsy and were proved to have BPH, were enrolled in this study. We developed an equation for continuously coded TPV and a logistic regression-based nomogram for estimating a TPV grater than 40 mh Predictive accuracy and performance characteristics were assessed using an area under the receiver operating characteristics curve （AUC） and calibration plots. The final linear regression model indicated age, PSA, %fPSA and BW as independent predictors of continuously coded TPV. For predictions in the training set, the multiple correlation coefficient was increased from 0.38 for PSA alone to 0.60 in the final model. We developed a novel nomogram incorporating age, PSA, %fPSA and BW for estimating TPV greater than 40 mh External validation confirmed its predictive accuracy, with AUC value of 0.764. Calibration plots showed good agreement between predicted probability and observed proportion. In conclusion, TPV can be easily estimated using these four independent predictors.

关键词： body weight equation nomogram prediction prostate-specific antigen prostate volume

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Immunohistochemical Study of Serum Proteins in Normal and Cadmium-Treated Mouse Testis by in vivo Cryotechnique

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Journal of Reproduction and Contraception 2005年第3期16卷 137-146页

作者： Nobuo TERADA Nobuhiko OHNO yasuhisa fujii Shinichi OHNO Department of Anatomy Interdisciplinary Graduate School of Medicine and Engineering University of Yamanashi Yamanashi 409-3898 Japan

Objective To investigate time-dependent changes of serum proteins permeability in the normal and cadmium（Cd）-treated mouse testis, reflecting tight junctional （TJ） barriers of Sertoli cells Methods The serum proteins, albumin and immunoglobulin-G（IgG）, in the seminiferous tubules were firstly immobilized by the “in vivo cryotechnique”, in which the dynamic blood circulation was always kept. The cryofixed testicular tissues were then processed for the freeze-substitution method, and embedded in the paraffin wax. Serial sections of 5μm thickness were immunostained by anti-mouse albumin or IgG antibody with peroxidase immunostaining, and also stained with hematoxylin-eosine （HE）for morphological observation. Results In normal seminiferous tubules, albumin immunoreaction products were localized around peritubular myoid cells and among Leydig cells, as well as in blood vessels. They were also localized as arch-like patterns around some spermatogonia in basal compartments. The number of the immunopositive arch structures was different according to developmental stages of the seminiferous cycle, judging from the arrangement of germ cells by HE-staining. The patterns of localization of IgG immunostaining in normal mouse testis were similar to that of albumin. In 24 h after Cd-treatment, some enlarged spaces and vesicular formation in the seminiferous epithelium were observed on the paraffin sections by HE-staining. The albumin or IgGimmunolocalization was seen not only in the basal compartments, but also in the adluminal compartments between *** and germ cells. Conclusion The structural changes of inter-Sertoli TJ barriers in vivo, such as different immunostaining patterns of serum proteins between the normal and Cd-treated mouse seminiferous tubules, could be clearly detected by the “in vivo cryotechnique” with albumin or IgG immunohistochemistry.

关键词： immunohistochemistry in vivo cryotechnique serum proteins testis cadmium

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Diffusion-weighted magnetic resonance imaging in management of bladder cancer, particularly with multimodal bladder-sparing strategy

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World Journal of Radiology 2014年第6期6卷 344-354页

作者： Soichiro Yoshida Fumitaka Koga Shuichiro Kobayashi Hiroshi Tanaka Shiro Satoh yasuhisa fujii Kazunori Kihara Department of Urology Tokyo Medical and Dental University Graduate School Tokyo 113-8677 Japan Department of Urology Tokyo Metropolitan Cancer and Infectious Diseases Center Komagome Hospital Tokyo 113-0021 Japan Department of Radiology Ocha-nomizu Surugadai Clinic Tokyo 101-0062 Japan

Bladder-sparing strategy for muscle-invasive bladdercancer(MIBC) is increasingly demanded instead ofradical cystectomy plus urinary diversion. Multimodaltherapeutic approaches consisting of transurethralresection, chemotherapy, radiotherapy and/or partialcystectomy improve patients' quality of life by preserv-ing their native bladder and sexual function withoutcompromising oncological outcomes. Because a favor-able response to chemoradiotherapy(CRT) is a prereq-uisite for successful bladder preservation, predictingand monitoring therapeutic response is an essentialpart of this approach. Diffusion-weighted magneticresonance imaging(DW-MRI) is a functional imagingtechnique increasingly applied to various types of can-cers. Contrast in this imaging technique derives fromdifferences in the motion of water molecules among tissues and this information is useful in assessing the biological behavior of cancers. Promising results in pre-dicting and monitoring the response to CRT have been reported in several types of cancers. Recently, growing evidence has emerged showing that DW-MRI can serve as an imaging biomarker in the management of blad-der cancer. The qualitative analysis of DW-MRI can be applied to detecting cancerous lesion and monitoring the response to CRT. Furthermore, the potential role of quantitative analysis by evaluating apparent diffu-sion coefficient values has been shown in character-izing bladder cancer for biological aggressiveness and sensitivity to CRT. DW-MRI is a potentially useful tool for the management of bladder cancer, particularly in multimodal bladder-sparing approaches for MIBC.

关键词： Diffusion magnetic resonance imaging Bladder cancer Urothelial carcinoma Chemotherapy Radiotherapy

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体内冷冻的正常及镉处理小鼠睾丸内血清蛋白质的免疫组织化学研究

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生殖与避孕 2006年第3期26卷 135-141页

作者：廖晓岗 Nobuo TERADA 李子龙 Nobuhiko OHNO yasuhisa fujii Shinichi OHNO 重庆医科大学电子显微镜室重庆400016 山梨大学医学工学综合研究部解剖学系中国医科大学第一附属医院沈阳110001

目的:了解正常和镉(Cd)处理小鼠睾丸内血清蛋白质渗透性的时相性变化,揭示支持细胞间紧密连接的屏障功能。方法:保持正常动力学的血循环下,运用活体内冷冻技术对曲细精管内白蛋白和IgG进行快速冷冻固定。冷冻固定的睾丸行冷冻置换后作... 详细信息

目的:了解正常和镉(Cd)处理小鼠睾丸内血清蛋白质渗透性的时相性变化,揭示支持细胞间紧密连接的屏障功能。方法:保持正常动力学的血循环下,运用活体内冷冻技术对曲细精管内白蛋白和IgG进行快速冷冻固定。冷冻固定的睾丸行冷冻置换后作石蜡包埋。5μm厚连续切片分别用于抗鼠血清白蛋白和IgG的免疫组化染色和H.E.染色。结果:正常曲细精管,白蛋白免疫反应产物主要定位于管周肌样细胞周围,以及间质细胞之间和毛细血管内,部分免疫反应产物呈拱型出现在曲细精管上皮基底室内,沿精原细胞表面分布。在依据生精细胞排列而区分的曲细精管上皮周期的不同发育时相,拱型免疫反应产物的数量不同。正常小鼠睾丸内IgG免疫反应的定位型式与白蛋白的免疫定位相似。Cd处理后24h,部分曲细精管上皮内空泡形成和管腔增大,白蛋白和IgG免疫反应产物不仅出现在基底室,而且也见于近腔室的支持细胞和生精细胞之间。结论:运用活体内快速冷冻技术结合血清白蛋白和IgG的免疫组化方法,能清楚地揭示正常和Cd处理小鼠曲细精管内血清蛋白质的不同免疫染色型式,反映体内支持细胞间紧密连接结构的变化。

关键词：免疫组化活体内冷冻技术血清蛋白质睾丸镉(Cd)

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活体冷冻固定及冷冻置换的小鼠睾丸组织内血清白蛋白和IgG的免疫组织化学定位

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中国组织化学与细胞化学杂志 2006年第3期15卷 251-255页

作者：廖晓岗 Nobuo Terada 李子龙 Nobuhiko Ohno yasuhisa fujii Shinichi Ohno 重庆医科大学电子显微镜室重庆400016 山梨大学医学工学综合研究部解剖学讲座中国医科大学第一附属医院沈阳110001

目的了解小鼠睾丸组织内血清白蛋白和IgG渗透性的时相性变化,揭示支持细胞间紧密连接的屏障功能。方法运用活体内冷冻固定技术对小鼠睾丸进行快速冷冻固定和冷冻置换,并作石蜡包埋。5μm厚连续切片用于抗鼠血清白蛋白和IgG的免疫组化染... 详细信息

目的了解小鼠睾丸组织内血清白蛋白和IgG渗透性的时相性变化,揭示支持细胞间紧密连接的屏障功能。方法运用活体内冷冻固定技术对小鼠睾丸进行快速冷冻固定和冷冻置换,并作石蜡包埋。5μm厚连续切片用于抗鼠血清白蛋白和IgG的免疫组化染色和H.E染色。结果H.E染色切片显示距冷冻组织表面300-400μm深度范围内的精曲小管结构保存完好,无明显冰晶形成所致的人工损伤。血清白蛋白和IgG免疫反应产物主要定位于精曲小管管周肌样细胞周围,以及间质细胞之间和毛细血管内,部分免疫反应产物呈拱型出现在精曲小管上皮内,并严格限定在基底室,沿精原细胞表面分布。拱型免疫反应产物的数量与精曲小管上皮周期的发育时相密切相关。结论活体内快速冷冻固定和冷冻置换结合血清白蛋白和IgG的免疫组化方法,可很好地保存精曲小管组织结构并清楚地揭示支持细胞间紧密连接结构的时相变化。

关键词：免疫组化活体内冷冻技术冷冻置换白蛋白 IgG 睾丸

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Association of a Tobacco-specific Nitrosamine Carcinogen with Urinary Cotinine,Urinary Sodium Excretion,and Total Energy Intake in Adolescents and Children

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Current Medical Science 2021年第2期41卷 270-278页

作者： Jong Weon Choi Tatsuyoshi fujii Noriyoshi fujii Department of Laboratory Medicine College of MedicineInha UniversityIncheon 22332Republic of Korea Deparment of Intermnal Medicine Tsukuba University Hospital Mito Clinical Education and Training CenterMito Kyodo General HospitalMito 310-0015Japan School of Medicine School of Medicine and Medical SciencesUniversity of TsukubaTsukuba 305-8577Japan

This study investigated the association of a tobacco-specific nitrosamine carcinogen,4-(methylnitrosamino)-1-(3-pyridy1)-I-butanol(NNAL)with urinary cotinine(uCot),urinary sodium(uNa)excretion,systolic blood pressure(sBP),and total energy intake in adolescents and children in relation to the subjects'age.A total of 790 subjects aged 6-19 years were ***,uCot,corrected NNAL(cNNAL),the NNAL/uCot ratio,uNa,sBP,and nutrient intake were measured.A strong association between uCot and cNNAL was observed in children who were 11 years of age(r=0.881,P<0.001);however,no significant association was noted in adolescents who were 19 years of *** uNa level was significantly higher(133.9 mmol/L vs.107.8 mmol/L,P<0.001)and sBP was significantly lower(105.3 mmHg vs.110.6 mmHg,P=0.012)in adolescents with elevated NNAL than in those without elevated *** was significantly higher in subjects with increased uNa excretion than in those without increased uNa *** was positively correlated with uNa(r=0.183,P<0.001)and negatively correlated with sBP(r=0.142,P<0.001).Non-smokers with elevated NNAL/uCot ratios had significantly lower total energy intake than those without elevated NNAL/uCot ratios(1729.0 kcal/day vs.1911.0 kcal/day,P=0.008).The relationship between NNAL and uCot varied according to the subjects'*** seems to play a role in decreasing sBP by enhancing uNa *** nutrient intake may contribute to endogenous formation of NNAL in non-smoking adolescents and children.

关键词： nitrosamine cotinine urinary sodium excretion nutrient intake blood pressure

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Interface strengthening in dissimilar double-sided friction stir spot welding of AZ31/ZK60 magnesium alloys by adjustable probes

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Journal of Materials Science & Technology 2021年第26期85卷 158-168页

作者： Xiaopei Wang Yoshiaki Morisada Hidetoshi fujii Joining and Welding Research Institute Osaka University11-1 MihogaokaIbarakiOsaka 567-0047Japan

Dissimilar welding of AZ31/ZK60 magnesium alloys with a thickness of 2 mm was successfully carried out by the double-sided friction stir spot welding with adjustable probes.A dissimilar joint bearing flat surfaces on both sides without a keyhole was obtained and the shear failure load of 8.7±0.5 kN was *** role of the adjustable probes has been revealed in *** the center of the stir zone,the welding interface structure was heterogeneous around which some distinct oxides still remained,leading to a weak interface *** the contrary,the welding interface structure around the shoulder/probe interface was homogeneous with no oxides giving rise to a strong interface strength,which is attributed to the severe material flow introduced by the adjustable *** addition,the vicinity outside the shoulder/probe interface,where the fracture occurred during the shear tensile tests,was also strengthened owing to the shearing and torsion by the adju stable ***,a stable plug failure can be obtained and the joint properties can be improved.

关键词： Friction stir spot welding Dissimilar welding Interface strengthening Microstructure Magnesium alloys

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Redox regulation of fertilisation and the spermatogenic process

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Asian Journal of Andrology 2011年第3期13卷 420-423,511,512页

作者： Junichi fujii Satoshi Tsunoda Department of Biochemistry and Molecular Biology Graduate School of Medical Science Yamagata University Yamagata 990-9585 Japan Respiratory and Cardiovascular Diseases Research Center Research institute for Advanced Molecular Epidemiology Yamagata University Yamagata 990-9585 Japan Global COE Program for Medical Sciences Japan Society for the Promotion of Science Tokyo Japan

Oxidative stress is one of the major causes of male infertility; it damages spermatogenic cells, the spermatogenic process and sperm function. Recent advances in redox biology have revealed the signalling role of reactive oxygen species （ROS） that are generated by cells. While highly reactive oxidants, such as the hydroxyl radical, exert largely deleterious effects, hydrogen peroxide can feasibly serve as a signal mediator because it is moderately reactive and membrane permeable and because it can oxidize only limited numbers of functional groups of biological molecules. The amino acid side chain most sensitive to oxidation is cysteine sulphydryl, which is commonly involved in the catalysis of some enzymes. Although the reactivity of cysteine sulphhydryl is not very high in ordinary proteins, some phosphatases possess a highly reactive sulphydryl group at their catalytic centre and are thereby oxidatively inactivated by transiently elevated hydrogen peroxide levels after extracellular stimuli and under certain environmental conditions. Peroxiredoxins, in turn, show moderate hydrogen peroxide-reducing activity, and their role in the modulation of ROS-mediated signal transduction in ordinary cells, mediated by protecting phosphatases from oxidative inactivation, has attracted much attention. Although knowledge of the signalling role of ROS in the male reproductive system is limited at present, its significance is becoming a focal issue. Here, we present a review of the emerging signalling role of hydrogen peroxide in testes.

关键词： hydrogen peroxide oxidative stress peroxiredoxin signal transduction

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Charge and Heat Transport in Polycrystalline Metallic Nanostructures

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Chinese Physics Letters 2008年第9期25卷 3360-3363页

作者：张兴 TAKAHASHI Koji fujii Motoo Key Laboratory for Thermal Science and Power Engineering of Ministry of Education Department of Engineering Mechanics Tsinghua University Beijing 100084 Graduate School of Engineering Kyushu University Fukuoka 819-0395 Japan Research Center for Hydrogen Industrial Use and Storage AIST Fhkuoka 819-0395 Japan

Metals are typically good conductors in which the abilities to transport charge and to transport heat can be related through the Wiedemann-Franz law. Here we report on an abnormal charge and heat transport in polycrystalline metallic nanostructures in which the ability to transport charge is weakened more obviously than that to transport heat. We attribute it to the influence of the internal grain boundaries and have formulated a novel relation to predict the thermal conductivity. The Wiedemann-Franz law is then modified to account for the influence of the grain boundaries on the charge and heat transport with the predictions now agreeing well with the measured results.

关键词： the power-law exponents precipitation durative abrupt precipitation change

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Integrating the effect of abutments in estimating the average vertical stress of elastic hard rock pillars by combining numerical modelling and artificial neural networks

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Underground Space 2023年第6期13卷 121-135页

作者： Nevaid Dzimunya Yoshiaki fujii Youhei Kawamura Faculty of Engineering Hokkaido UniversitySapporo N13W8Japan

Estimating average vertical pillar stresses is a critical step in designing room-and-pillar *** analytical methods can be used to estimate the vertical stresses acting on the ***,the present analytical methods fail to adequately account for the influence of abutments on the distribution of vertical stresses,especially when applied to narrow panel widths and pillar layouts comprising evenly spaced *** this study,a multi-layer perceptron neural network(MLPNN)was applied to predict the vertical loads of regular pillars more *** of room-and-pillar mine layouts were modeled using a displacement discontinuity method(DDM),and a database of 2355 sampled pillar cases was *** MLPNN was trained based on this database,and its prediction capabilities were further validated using simulations by a finite difference code(i.e.,FLAC3D).The model predictions and the FLAC3D simulations reasonably agreed with a regression coefficient of *** model was also adapted for mine cases with evenly spaced barrier pillars,and its application to a real case study mine has shown to provide accurate pillar stress estimations;hence,this model is suitable for practical use at *** though the MLPNN model cannot be applied universally to all mine situations,it seems as a significant improvement over existing analytical techniques in terms of accounting for the influence of abutments on pillar stresses.

关键词： Pillar stress Abutments Multi-layer perceptron neural network Numerical simulation Room-and-pillar mine

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