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Biosynthesis of raw starch degradingβ-cyclodextrin glycosyltransferase by immobilized cells of Bacillus licheniformis using potato wastewater

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BIOCELL 2021年第6期45卷 1661-1672页

作者： yasser S.MOSTAFA SAAD A.ALAMRI SULIMAN A.ALRUMMAN TAREK H.TAHA MOHAMED HASHEM MAHMOUD moustafa LAMIAA I.FAHMY Department of Biology College of ScienceKing Khalid UniversityAbha9004Saudi Arabia Prince Sultan Bin Abdulaziz Center for Environmental and Tourism Research and Studies King Khalid UniversityAbha9004Saudi Arabia Environmental Biotechnology Department Genetic Engineering and Biotechnology Research InstituteCity of Scientific Research&Technological ApplicationsNew Borg El-Arab City21934Egypt Department of Botany and Microbiology Faculty of ScienceAssiut UniversityAssiut71515Egypt Department of Botany and Microbiology Faculty of ScienceSouth Valley UniversityQena83523Egypt Department of Microbiology and Immunology Faculty of PharmacyOctober University for Modern Science and ArtGiza12451Egypta

The study was sought to enhance the synthesis of thermal stableβ-cyclodextrin glycosyltransferase(β-CGTase)using potato wastewater as a low-cost medium and assess the degree to which it is efficient for industrial production ofβ-cyclodextrin(β-CD)from raw potato *** bacteria producingβ-CGTase was isolated from Saudi Arabia and the promising strain was identified as Bacillus licheniformis using phylogenetic analysis of the 16S rRNA ***-encapsulated cultures exhibited twice-fold ofβ-CGTase production more than free *** electron microscopy(SEM)of polymeric capsules indicated the potential for a longer shelf-life,which promotes the restoration of activity in bacterial cells across semi-continuous fermentation ofβ-CGTase production for 252 *** optimal conditions forβ-CGTase synthesis using potato wastewater medium were at 36 h,pH of 8.0,and 50°C with 0.4%potato starch and 0.6%yeast extract as carbon and nitrogen sources,*** purified enzyme showed a specific activity of 63.90 U/mg with a molecular weight of∼84.6 kDa as determined by SDS-PAGE *** high enzyme activity was observed up to 60°C,and complete stability was achieved at 75°*** levels of activity and stability were shown at pH 8.0,and the pH range from 7.0–10.0,*** enzyme has an appreciable affinity for raw potato starch with a Km of 5.7×10−6 M and a Vmax of 87.71μmoL/mL/min.β-CD production was effective against 25 U/g of raw potato *** outcomes demonstrated its feasibility to develop a fermentation process by integrating the cost-effective production ofβ-CGTase having distinctive properties forβ-CD production with ecofriendly utilization of potato wastewater.

关键词： Potato wastewater Bacillus licheniformis β-CGTase 16S rDNA gene Semi-continuous fermentation β-cyclodextrin

Evaluation of the microstructure,thermal and mechanical properties of Cu/SiC nanocomposites fabricated by mechanical alloying

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International Journal of Minerals,Metallurgy and Materials 2021年第3期28卷 475-486页

作者： Essam B.moustafa Mohammed A.Taha Mechanical Engineering Departments Faculty of EngineeringKing Abdulaziz UniversityJeddah 21589Saudi Arabia Solid State Physics Department National Research CentreEl Buhouth St.Dokki12622 GizaEgypt

Nano-sized silicon carbide(SiC:0wt%,1wt%,2wt%,4wt%,and 8wt%)reinforced copper(Cu)matrix nanocomposites were manufactured,pressed,and sintered at 775 and 875℃in an argon atmosphere.X-ray diffraction(XRD)and scanning electron microscopy were performed to characterize the microstructural *** density,thermal expansion,mechanical,and electrical properties were *** analyses showed that with increasing SiC content,the microstrain and dislocation density increased,while the crystal size *** coefficient of thermal expansion(CTE)of the nanocomposites was less than that of the Cu *** improvement in the CTE with increasing sintering temperature may be because of densification of the ***,the mechanical properties of these nanocomposites showed noticeable enhancements with the addition of SiC and sintering temperatures,where the microhardness and apparent strengthening efficiency of nanocomposites containing 8wt%SiC and sintered at 875℃were 958.7 MPa and 1.07 vol%^(−1),*** electrical conductivity of the sample slightly decreased with additional SiC and increased with sintering *** prepared Cu/SiC nanocomposites possessed good electrical conductivity,high thermal stability,and excellent mechanical properties.

关键词： copper matrix nanocomposites silicon carbide coefficient of thermal expansion elastic moduli electrical conductivity mechanical alloying

Geotectonic significance of the Neoproterozoic ophiolitic metagabbros of Muiswirab area,South Eastern Desert,Egypt:constraints from their mineralogical and geochemical characteristics

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Acta Geochimica 2020年第6期39卷 887-910页

作者： moustafa M.Mogahed Wlaa Mahmoud Saad Geology Department Faculty of ScienceBenha University-EgyptP.O.Box 13518BenhaEgypt

Petrological and geochemical studies of Neo-proterozoic metagabbros were carried out in the Muiswirab area,South Eastern Desert of *** Muiswirab area comprises of ophiolitic metagabbroic rocks(MOM),which are tectonically thrusted over a thick pile of metavolcanic rocks and intruded by syn-and post-tectonic granitoid *** whole-rock geochemical variations coupled with chemical compositions of mineral constituents are used to attain the genesis and tectonic evolution of the studied *** geothermobarometric investiga-tion of the analyzed amphiboles from(MOM)revealed that these metagabbros underwent regional metamorphism under lower to upper greenschist facies(biotite zone)conditions(at a temperature of 450 to 500℃and pressure of 1-3 kbar).Geochemically,the metagabbros(MOM)show tholeiitic affinity and cxhibiting both arc-and MORB-like characters as evidenced by their clinopyrox-ene compositions and the Ti/V ratios(11.84-31.65),which considered as prominent features of forearc tectonic *** geochemical features suggest a probable fractionation of olivine±clinopyroxene±plagioclase as well as insignificant crustal *** parental magma of the investigated MOM rocks seems to be developed in a sub-arc mantle wedge setting due to the enrichments of LILE(e.g..Rb,Ba,Sr,Pb)over HFSE(e.g,Ti,Nb,Y,Zr,Hf,Ta).The studied MOM rocks have lower values of Nb/U relative to MORB and OIB indicating that their geochemical variation produced due to the enrichment of a lithosphere mantle by OIB like *** ratios of Zn/Fet,La/Sm,Sm/Yb,Th/Yb and Nb/Yb indicate that the MOM rocks represent a fragment of oceanic crust originated at a supra-subduction zone environment and their parental magma developed by 5-30%partial melting of a spinel lherzolite mantle rather than pyroxenite in an island arc setting and conformable with most of the Egyptian ophiolitic metagabbros.

关键词： Egypt Eastern Desert Muiswirab area Ophiolitic metagabbros N-MORB Arc tholeiites Foreare tectonic regime Volcanic-arc setting

Relative performance of novel blast wave mitigation system to conventional system based on mitigation percent criteria

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Defence Technology（防务技术） 2021年第3期17卷 912-922页

作者： moustafa Esa Mostafa S.Amin Ahmed Hassan Eng.Mechanics Dept. Military Technical CollegeKobry ElkobbahCairoEgypt Civil Eng.Dept. Military Technical CollegeKobry ElkobbahCairoEgypt

Recent researches focused on developing robust blast load mitigation systems due to the threats of terrorist *** of the main embraced strategies is the structural systems that use mitigation *** are developed from a combination of structural elements and described herein as conventional *** the promising techniques is that redirect the waves propagation through hollow *** blast wave propagation through tubes provides an efficient system since it combines many blast wave phenomena,such as reflection,diffraction,and *** this research,a novel blast load mitigation system,employed as a protection fence,is developed using a technique similar to the technique of the bent tube in manipulating the *** relative performance of the novel system to the conventional system is evaluated based on mitigation percent *** of both systems are calculated through numerical *** proposed novel system proved to satisfy high performance in mitigating the generated blast waves from charges weight up to 500 kg TNT at relatively small standoff distances(5 m and 8 m).It mitigates at least 94%of the blast waves,which means that only 6%of that blast impulse is considered as the applied load on the targeted structure.

关键词： Blast mitigation Blast barriers Protection system Mitigation percent Protection evaluation

Emerging small-molecule therapeutic approaches for Alzheimer's disease and Parkinson's disease based on targeting microRNAs

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Neural Regeneration Research 2022年第2期17卷 336-337页

作者： Somaya A.Abdelrahman moustafa T.Gabr Department of Medicinal Chemistry Faculty of PharmacyMansoura UniversityMansouraEgypt Department of Radiology Stanford University School of MedicineStanfordCAUSA

The role of micro RNAs in the progression of neurodegenerative diseases: Micro RNAs(mi RNAs) are endogenous, non-coding and short RNA nucleotides that regulate gene expression through base pairing with the targeted me... 详细信息

关键词： endogenous Alzheimer diseases

Framework for COVID-19 Segmentation and Classification Based on Deep Learning of Computed Tomography Lung Images

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Journal of Electronic Science and Technology 2022年第3期20卷 246-256页

作者： Wessam M.Salama moustafa H.Aly the Department of Basic Science Faculty of EngineeringPharos UniversityAlexandria12455Egypt the Department of Electronics and Communications Engineering College of Engineering and TechnologyArab Academy for ScienceTechnology and Maritime TransportAlexandria1029Egypt

Corona Virus Disease 2019(COVID-19) has affected millions of people worldwide and caused more than6.3 million deaths(World Health Organization, June 2022). Increased attempts have been made to develop deep learning methods to diagnose COVID-19 based on computed tomography(CT) lung images. It is a challenge to reproduce and obtain the CT lung data, because it is not publicly available. This paper introduces a new generalized framework to segment and classify CT images and determine whether a patient is tested positive or negative for COVID-19 based on lung CT images. In this work, many different strategies are explored for the classification ***50 and VGG16 models are applied to classify CT lung images into COVID-19 positive or negative. Also,VGG16 and ReNet50 combined with U-Net, which is one of the most used architectures in deep learning for image segmentation, are employed to segment CT lung images before the classifying process to increase system performance. Moreover, the image size dependent normalization technique(ISDNT) and Wiener filter are utilized as the preprocessing techniques to enhance images and noise suppression. Additionally, transfer learning and data augmentation techniques are performed to solve the problem of COVID-19 CT lung images deficiency, therefore the over-fitting of deep models can be avoided. The proposed frameworks, which comprised of end-to-end, VGG16,ResNet50, and U-Net with VGG16 or ResNet50, are applied on the dataset that is sourced from COVID-19 lung CT images in Kaggle. The classification results show that using the preprocessed CT lung images as the input for U-Net hybrid with ResNet50 achieves the best performance. The proposed classification model achieves the 98.98%accuracy(ACC), 98.87% area under the ROC curve(AUC), 98.89% sensitivity(Se), 97.99 % precision(Pr), 97.88%F-score, and 1.8974-seconds computational time.

关键词： Augmentation classification computed tomography(CT) Corona Virus Disease 2019(COVID-19) deep learning ResNet50 segmentation U-Net VGG16

Constraints of Mantle and Crustal Sources Interaction during Orogenesis of Pre-and Post-collision Granitoids from the Northern Arabian-Nubian Shield:A Case Study from Wadi El-Akhder Granitoids,Southern Sinai,Egypt

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Acta Geologica Sinica(English Edition) 2021年第5期95卷 1527-1550页

作者： moustafa M.MOGAHED Khaled M.ABDELFADIL Geology Department Faculty of ScienceBenha UniversityBenha 13518Egypt Geology Department Faculty of ScienceSohag UniversitySohag 82524Egypt

The Egyptian older and younger granitic rocks emplaced during pre-and post-collision stages of Neoproterozoic Pan-African orogeny,respectively,are widely distributed in the southern Sinai Peninsula,constituting 70% of the basement *** Wadi El-Akhder,southwestern Sinai,is a mountainous terrain exposing two granitoid suites,namely the Wadi El-Akhder Older Granites(AOG)and the Homra Younger Granites(HYG).The AOG(granodiorites with subordinate tonalite compositions)have geochemical characteristics of medium-K calc-alkaline,metaluminous to mildly peraluminous granitoids formed in an island-arc environment,which are conformable with well-known Egyptian older granitoids rocks,whereas the HYG display calc-alkaline to slightly alkaline nature,peraluminous syeno-,monzogranites and alkali feldspar granites matching well those of the Egyptian younger *** respect to the AOG granitoids,the HYG granites contain lower Al_(2)O_(3),FeO^(*),MgO,MnO,CaO,TiO_(2),Sr,Ba,and V,but higher Na_(2)O,K_(2)O,Nb,Zr,Th,and *** AOG are generally characterized by enrichment in LILE and LREE and depletion in HFSE relative to N-MORB values(e.g.,negative Nb and Ta anomalies).The geochemical features of the AOG follow assimilation-fractional crystallization(AFC)trends indicative of extensive crustal contamination of magma derived from a mantle *** chemical characteristics of the AOG are remarkably similar to those of subduction-related granitoids from the ArabianNubian Shield(ANS).The compositional variations from monzogranites through syenogranites to alkali feldspar granite within HYG could not be explained by fractional crystallization *** the whole-rock composition of the HYG to melts generated by experimental dehydration melting of meta-sedimentary and magmatic rocks reveals that they appear to be derived by extended melting of psammitic and pelitic metasediments,which is similar to the most of younger granitic suites in the ANS.

关键词： older and younger granitoids mantle-crust interaction assimilation-fractional crystallization(AFC) southern Sinai Arabian-Nubian Shield

Bacterial diversity in Fez tanneries and Morocco's Binlamdoune River,using 16S RNA gene based fingerprinting

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Journal of Environmental Sciences 2010年第12期22卷 1944-1953页

作者： Adil Essahale moustafa Malki Irma Marín Mohieddine Moumni Moulay Isml University Faculty of ScienceDepartment of BiologyBP 11 201 ZitouneMeknès 50000Morocco Department of Molecular Biology Faculty of ScienceAutonomous University of Madrid (UAM)

Tannery wastewater causes serious ecological and sanitary damage. Chemical analysis of water from Binlamdoune River of the medina of Fez was conducted and the results revealed the presence of toxic elements from tanneries and other industrial activities, which strongly affected water quality. To determine the effectiveness of bioremediation for depollution, we studied the abundance and diversity of bacteria residing in these polluted environments. Conducting denaturing gradient gel electrophoresis （PCR-DGGE） of the 16S rDNA area using primers related to bacteria showed a bacterial community belonging to eubacterial groups, that is, Epsilonproteobacteria, Clostridia, Lactobacillales, Bacteroidetes, Gammaproteobacteria, and Alphaproteobacteria. In addition, cloning displayed the presence of clones belonging to the Firmicutes group. Moreover, scanning electron microscopy revealed a significant heterogeneity of microorganism forms and structures. These endogenous microbes could have a significant role in the purification of Binlamdoune River and Fez tannery wastewater.

关键词： Fez tanneries Binlamdoune River biodiversity bacteria 16S rRNA

Deterministic and Stochastic Fractional Order Model for Lesser Date Moth

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Computer Systems Science & Engineering 2022年第2期40卷 749-764页

作者： moustafa El-shahed Asmaa M.Al-Dububan Department of Mathematics Unaizah College of Sciences and ArtsQassim UniversityP.O.Box 3771Unaizah51911Saudi Arabia Department of Mathematics Buraydah College of Sciences and ArtsQassim UniversityP.O.Box 1162Buraydah51431Saudi Arabia

In this paper,a deterministic and stochastic fractional order model for lesser date moth(LDM)using mating disruption and natural enemies is proposed and *** interaction between LDM larvae,fertilized LDM female,unfertilized LDM female,LDM male and the natural enemy is *** order to clarify the characteristics of the proposed deterministic fractional order model,the analysis of existence,uniqueness,non-negativity and boundedness of the solutions of the proposed fractional-order model are *** addition,some sufficient conditions are obtained to ensure the local and global stability of equilibrium *** occurrence of local bifurcation near the equilibrium points is investigated with the help of Sotomayor’s *** simulations are conducted to illustrate the properties of the proposed fractional order model with respect to the intrinsic growth rate of the LDM larvae,natural enemy’s mortality rate,predation rate,sex pheromone trap parameter,fractional order and environmental *** impact of mating disruption on lesser date moth is ***,a numerical approximation method is developed for the proposed stochastic fractional-order model.

关键词： Lesser date moth stochastic stability natural enemies Sotomayor’s theorem mating disruption