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Bacterial infections post-living-donor liver transplantation in Egyptian hepatitis C virus-cirrhotic patients: A singlecenter study

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World Journal of Hepatology 2017年第20期9卷 896-904页

作者： mohamed F montasser Nadia A Abdelkader Sara m Abdelhakam Hany Dabbous Iman F montasser yasmine m massoud Waleed Abdelmoaty Shereen A Saleh mohamed Bahaa Hany Said mahmoud El-meteini Department of Tropical medicine Faculty of Medicine Ain Shams University Department of Internal medicine Faculty of Medicine Ain Shams University Department of Hepatobiliary Surgery Ain Shams Center for Organ Transplant Faculty of Medicine Ain Shams University

AIm To determine risk factors, causative organisms and antimicrobial resistance of bacterial infections following living-donor liver transplantation(LDLT) in cirrhotic *** This prospective study included 45 patients with hepatitis C virus-related end-stage liver disease who underwent LDLT at Ain Shams Center for Organ Transplant, Cairo, Egypt from January 2014 to November 2015. Patients were followed-up for the first 3 mo after LDLT for detection of bacterial infections. All patients were examined for the possible risk factors suggestive of acquiring infection pre-, intra-and post-operatively. Positive cultures based on clinical suspicion and patterns of antimicrobial resistance were identified. RESULTS Thirty-three patients(73.3%) suffered from bacterial infections; 21 of them had a single infection episode, and 12 had repeated infection episodes. Bile was the most common site for both single and repeated episodes of infection(28.6% and 27.8%, respectively). The most common isolated organisms were gramnegative bacteria. Acinetobacter baumannii was the most common organism isolated from both single and repeated infection episodes(19% and 33.3%, respectively), followed by Escherichia coli for repeated infections(11.1%), and Pseudomonas aeruginosa for single infections(19%). Levofloxacin showed high sensitivity against repeated infection episodes(P = 0.03). Klebsiella, Acinetobacter and Pseudomonas were multi-drug resistant(mDR). Pre-transplant hepatocellular carcinoma(HCC) and duration of drain insertion(in days) were independent risk factors for the occurrence of repeated infection episodes(P = 0.024).CONCLUSION mDR gram-negative bacterial infections are common post-LDLT. Pre-transplant HCC and duration of drain insertion were independent risk factors for the occurrence of repeated infection episodes.

关键词： Living-donor liver transplantation Bacterial infection multi-drug resistance Hepatitis C virus Liver cirrhosis

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An Adaptive Hate Speech Detection Approach Using Neutrosophic Neural Networks for Social media Forensics

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Computers, materials & Continua 2024年第4期79卷 243-262页

作者： yasmine m.Ibrahim Reem Essameldin Saad m.Darwish Department of Information Technology Institute of Graduate Studies and ResearchAlexandria UniversityAlexandria21526Egypt Faculty of Computers and Information Technology Egyptian E-Learning University(EELU)Giza12611Egypt Faculty of Computers and Data Science Alexandria UniversityAlexandria21554Egypt

Detecting hate speech automatically in social media forensics has emerged as a highly challenging task due tothe complex nature of language used in such platforms. Currently, several methods exist for classifying hatespeech, but they still suffer from ambiguity when differentiating between hateful and offensive content and theyalso lack accuracy. The work suggested in this paper uses a combination of the Whale Optimization Algorithm(WOA) and Particle Swarm Optimization (PSO) to adjust the weights of two multi-Layer Perceptron (mLPs)for neutrosophic sets classification. During the training process of the mLP, the WOA is employed to exploreand determine the optimal set of weights. The PSO algorithm adjusts the weights to optimize the performanceof the mLP as fine-tuning. Additionally, in this approach, two separate mLP models are employed. One mLPis dedicated to predicting degrees of truth membership, while the other mLP focuses on predicting degrees offalse membership. The difference between these memberships quantifies uncertainty, indicating the degree ofindeterminacy in predictions. The experimental results indicate the superior performance of our model comparedto previous work when evaluated on the Davidson dataset.

关键词： Hate speech detection whale optimization neutrosophic sets social media forensics

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Trauma to the solid abdominal organs:The missed dark box of colonoscopy

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World Journal of Gastroenterology 2024年第7期30卷 624-630页

作者： mohamed H Emara Usama mazid yasmine A Elshaer mahmoud A Elkerdawy Dilaver Farooq malik Aya m mahros Department of Hepatology Gastroenterologyand Infectious DiseasesKafrelsheikh UniversityKafr-Elshikh 33516Egypt Department of medicine Alyousif HospitalAlkhobar 31952Saudi Arabia

Colonoscopy is an integral part of the lower bowel care and is generally considered a potentially safe diagnostic and therapeutic procedure performed as a daycare outpatient *** is associated with different complications that are not limited to adverse events related to the bowel preparation solutions used,the sedatives used,but to the procedure related as well including bleeding and *** to the extra-luminal abdominal organs during colonoscopy are uncommon,however,serious complications related to the procedure have been reported infrequently in the *** threatening injuries to the spleen,liver,pancreas,mesentery,and urinary bladder have been reported as early as in *** injuries should not be overlooked by clinicians and *** increasing abdominal pain,abdominal distension,and hemodynamic instability in absence of rectal bleeding should raise the possibility of severe organ *** and hepatic injury following colonoscopy are usually serious and may be life *** conservative management may help,yet they usually need interventional radiology or surgical *** pancreatitis following colonoscopy is usually mild and is mostly managed *** mechanism of abdominal organ injuries during colonoscopy is not fully understood,however many risk factors have been identified,which can be classified as-organ related,procedure related,and local abdominal *** colonoscopy and prior intra-abdominal adhesions are probably the most relevant risk factors for these *** lateral position,avoidance of looping and excessive force during the procedure would probably reduce the risk of such injuries.

关键词： Colonoscopy Pancreatitis Trauma Complications Adhesions

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multidrug-Resistant of Escherichia coli and Salmonella spp. Strains in Chicken Feces Intended for Consumption in Open Spaces of Ouagadougou, Burkina Faso

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Open Journal of Applied Sciences 2024年第4期14卷 881-892页

作者： Stéphanie Lynseh Carine Sita Bénao Dabiré Amana métuor Abdoul Karim Ouattara Rahimatou yasmine Wendkouni Tiemtoré Nicolas Ouédraogo Blandine Ouédraogo Rhaina Olivia Badini Lionel Eliada Benoit Bambara Serge Sougué Jacques Simporé Biomolecular and Genetic Laboratory (LABIOGENE) Pietro Annigoni Biomolecular Research Center (CERBA) Department of Biochemistry-Microbiology University Joseph Ki-Zerbo Ouagadougou Burkina Faso Department of Biochemistry-microbiology University of D&#233dougou D&#233dougou Burkina Faso National malaria Research and Training Centre (CNRFP) Ouagadougou Burkina Faso

Resistant bacteria can be transmitted to humans through feces or contaminated meat from local chickens. Bacterial strains were isolated from the intestinal contents of 400 local chicken samples from various sales sites. These strains were then characterized using bacteriological and biochemical methods to identify resistant strains. In a study conducted in Ouagadougou, we systematically collected chicken fecal samples from 20 locations across the city, followed by isolation and identification of Salmonella spp. using specific enrichment and culture methods, as well as Escherichia coli. Bacterial strains were characterized using antibiotic resistance profiles were determined through agar diffusion tests, revealing sensitivity or resistance to a range of antibiotics based on established scientific criteria. The results showed that out of the 400 samples collected, 81.25% and 63.5% were contaminated by Escherichia coli and Salmonella spp., respectively. Among these, 86.15% of identified Escherichia coli and 50.78% of Salmonella spp. displayed resistance to at least one tested antibiotic. Among 280 Escherichia coli isolates identified resistant to at least one antibiotic, 31.07% were resistant to cefotaxime (CTX), 20.35% to ceftazidime (CAZ), 21.07% to ceftriaxone (CTR), 75% to amoxicillin clavulanic acid (AmC), 23.57% aztreoname (ATm) and 27.14% were resistant to imipenem (ImP). In the case of the 129 Salmonella spp. isolates resistant to at least one tested antibiotic, 34.88% were resistant to CTX;41.08% to CAZ;35.65% to CTR, 92% to AmC, 39.53% to ATm and finally 47.28% were resistant to ImP. Our study revealed high prevalence of resistance in bacterial strains isolated from local chickens sold outdoors in Ouagadougou. These findings raise significant public health concerns, due to the possible transmission of these resistant strains to humans through the consumption of contaminated meat, thus complicating the treatment of bacterial infections.

关键词： multidrug-Resistant Chicken Ouagadougou Escherichia coli Salmonella spp. Antibiotic

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Retro Placental Hematoma: maternal and Fetal Prognosis at the maternity of the University Hospital of Bouake

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Open Journal of Obstetrics and Gynecology 2024年第1期14卷 44-56页

作者： Samake Yaya Djanhan Lydie Estelle menin-messou Benie michele Kouadio Kouadio Narcisse Akanji Iburaima Alamun m’bro Clausen Georgie Boko Dagoun Dagbesse Elysee Camara Sokhona N’guessan Kouadio Ismael Foua Bi Paul Hyacinthe Keita Ismael Vaho magnificat marina Kone minzata yasmine Doumbia Yacouba Gynaecology-Obstetrics Department Centre Hospitalier Universitaire de Bouaké Bouake Ivory Coast mother and Child Department Alassane Ouattara University Bouake Ivory Coast

Introduction: Retroplacental haematoma (RPH) is a very serious complication of pregnancy, with life-threatening consequences for both the mother and foetus. The aim of this study is to determine the incidence and epidemiological characteristics of patients with retroplacental haematoma (RPH) and describe the maternal-foetal complications at Bouaké University Hospital. methods: This was a cross-sectional prospective, descriptive and analytical study carried out at Bouaké University Hospital over a period of 3 years, from January 1, 2019 to December 31, 2021. All parturients with RPH whose delivery took place at the hospital were included in the study. Data were entered and analysed using EPI INFO software version 7.***.2.6. Results: We recorded 2,0959 deliveries, including 202 cases of RPH, representing an incidence of 0.96%. The 21 to 35 age group accounted for 64.4%, multigestas and large multigestas accounted for 58.5% and multiparas accounted for 41.6%. The main signs on clinical examination were metrorrhagia (100%), arterial hypertension (84.6%) and cervical cerclage (79.7%). Preeclampsia accounted for 50% of per-gestational pathologies. maternal mortality was 12.9%. morbidity was dominated by anaemia in 64.1%, followed by disseminated intravascular coagulation (DIC) in 21.8%, and the factors associated with this maternal prognosis were multiple gestations, multiparity, Sher grade IIIb and the occurrence of complications such as DIC, shock, renal complications and HELLP syndrome. Neonatal mortality was 79.2%, and the factors associated with these fetal prognoses were cup size ≥ 5 cm and hematoma weight ≥ 500 g. Conclusion: Better screening of at-risk populations, early diagnosis and treatment in an organised and equipped medical and surgical facility would improve prognosis.

关键词： Retroplacental Haematoma Lethality Bouaké and Prognosis

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Nature-Inspired Level Set Segmentation model for 3D-mRI Brain Tumor Detection

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Computers, materials & Continua 2021年第7期68卷 961-981页

作者： Oday Ali Hassen Sarmad Omar Abter Ansam A.Abdulhussein Saad m.Darwish yasmine m.Ibrahim Walaa Sheta Department of Computer Techniques Engineering Imam Al-Kadhum College(IKC)WasitIraq University of Information Technology and Communications Media Technology Engineering DepartmentIraq University of Information Technology and Communications Baghdad10081Iraq Department of Information Technology Institute of Graduate Studies and ResearchAlexandria University63 Horreya AvenueEl–ShatbyAlexandria21526Egypt Informatics Research Institute City of Scientific Research and Technology ApplicationsBorg EL Arab21934Egypt

medical image segmentation has consistently been a significant topic of research and a prominent goal,particularly in computer *** tumor research plays a major role in medical imaging applications by providing a tremendous amount of anatomical and functional knowledge that enhances and allows easy diagnosis and disease therapy *** prevent or minimize manual segmentation error,automated tumor segmentation,and detection became the most demanding process for radiologists and physicians as the tumor often has complex *** methods for detection and segmentation presently exist,but all lack high *** paper’s key contribution focuses on evaluating machine learning techniques that are supposed to reduce the effect of frequently found issues in brain tumor ***,attention concentrated on the challenges related to level set *** study proposed in this paper uses the Population-based Artificial Bee Colony Clustering(P-ABCC)methodology to reliably collect initial contour points,which helps minimize the number of iterations and segmentation errors of the level-set *** proposed model measures cluster centroids(ABC populations)and uses a level-set approach to resolve contour differences as brain tumors vary as they have irregular form,structure,and *** suggested model comprises of three major steps:first,pre-processing to separate the brain from the head and improves contrast ***,P-ABCC is used to obtain tumor edges that are utilized as an initial mRI sequence *** level-set segmentation is then used to detect tumor regions from all volume slices with fewer *** suggest improved model efficiency compared to state-of-the-art methods for both datasets BRATS 2019 and BRATS *** BRATS 2019,dice progress was achieved for Entire Tumor(WT),Tumor Center(TC),and Improved Tumor(ET)by 0.03%,0.03%,and 0.01%*** BRATS 2017,an increase in precision for WT was reac

关键词： 3D-mRI tumor diagnosis bio-inspired clustering ABC optimization multimodal detection

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VHH212 nanobody targeting the hypoxia-inducible factor 1α suppresses angiogenesis and potentiates gemcitabine therapy in pancreatic cancer in vivo

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Cancer Biology & medicine 2021年第3期18卷 772-787页

作者： Guangbo Kang min Hu He Ren Jiewen Wang Xin Cheng Ruowei Li Bo Yuan yasmine Balan Zixuan Bai He Huang Department of Biochemical Engineering School of Chemical Engineering&TechnologyTianjin UniversityTianjin 300350China Key Laboratory of Systems Bioengineering(ministry of Education) Tianjin UniversityTianjin 300072China Department of Gastroenterology Center of Tumor Immunology and CytotherapyMedical Research Center of The Affiliated Hospital of Qingdao UniversityQingdao 266003China Department of Chemical and Biological Engineering University of OttawaOttawa KIN 6N5Canada

Objective:We aimed to develop a novel anti-HIF-1αintrabody to decrease gemcitabine resistance in pancreatic cancer ***:Surface plasmon resonance and glutathione S-transferase pull-down assays were conducted to identify the binding affinity and specificity of anti-HIF-1αVHH212[a single-domain antibody(nanobody)].molecular dynamics simulation was used to determine the protein-protein interactions between hypoxia-inducible factor-1α(HIF-1α)and *** real-time polymerase chain reaction(PCR)and Western blot analyses were performed to identify the expressions of HIF-1αand VEGF-A in pancreatic ductal adenocarcinoma cell *** efficiency of the VHH212 nanobody in inhibiting the HIF-1 signaling pathway was measured using a dual-luciferase reporter ***,a PANC-1 xenograft model was developed to evaluate the anti-tumor efficiency of combined *** analysis was conducted to detect the expressions of HIF-1αand VEGF-A in tumor ***:VHH212 was stably expressed in tumor cells with low cytotoxicity,high affinity,specific subcellular localization,and neutralization of HIF-1αin the cytoplasm or *** binding affinity between VHH212 and the HIF-1αPAS-B domain was 42.7 n *** competitive inhibition of the HIF-1αheterodimer with an aryl hydrocarbon receptor nuclear translocator was used to inhibit the HIF-1/VEGF pathway in *** with single agent gemcitabine,co-treatment with gemcitabine and a VHH212-encoding adenovirus significantly suppressed tumor growth in the xenograft model with 80.44%tumor ***:We developed an anti-HIF-1αnanobody and showed the function of VHH212 in a preclinical murine model of PANC-1 pancreatic *** combination of VHH212 and gemcitabine significantly inhibited tumor *** results suggested that combined use of anti-HIF-1αnanobodies with first-line treatment may in the future be an effective treatment for pancreatic cancer.

关键词： Pancreatic cancer nanobody therapeutic intracellular antibody HIF-1αinhibitor gemcitabine chemosensitizer

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HepG2 cells support viral replication and gene expression of hepatitis C virus genotype 4 in vitro

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World Journal of Gastroenterology 2006年第30期12卷 4836-4842页

作者： mostafa K El-Awady Ashraf A Tabll yasmine S El-Abd mahmoud m Bahgat Hussein A Shoeb Samar S Youssef Noha G Bader El Din El-Rashdy m Redwan maha El-Demellawy moataza H Omran Wael T El-Garf Said A Goueli Department of Biomedical Technology National Research Center Tahrir Street PO 12622 Dokki Cairo Egypt Department of medicinal Chemistry Na- tional Research Center Tahrir Street PO 12622 Dokki Cairo Egypt Department of microbiology and Immunology Faculty of Pharmacy Cairo University Cairo Egypt Genetic Engineering and Biotechnology Research Institute New Borg El-Arab City Alexandria Egypt Department of Pathology School of medicine University of Wisconsin Madison WI United States

AIm： TO establish a cell culture system with longterm replication of hepatitis C virus （HCV） genome and expression of viral antigens in vitro. mETHODS： HepG2 cell line was tested for its susceptibility to HCV by incubation with a serum from a patient with chronic hepatitis C. Cells and supernatant were harvested at various time points during the culture. Culture supernatant was tested for its ability to infect na＇ive cells. The presence of minus （antisense） RNA strand, and the detection of core and E1 antigens in cells were examined by RT-PCR and immunological techniques （flow cytometry and Western blot） respectively. RESULTS： The intracellular HCV RNA was first detected on d 3 after infection and then could be consistently detected in both cells and supernatant over a period of at least three months. The fresh cells could be infected with supernatant from cultured infected cells. Flow cytometric analysis showed surface and intracellular HCV antigen expression using in house made polyclonal antibodies （anti-core, and anti-E1）. Western blot analysis showed the expression of a cluster of immunogenic peptides at molecular weights extended between 31 and 45 kDa in an one month old culture of infected cells whereas this cluster was undetectable in uninfected HepG2 cells. CONCLUSION： HepG2 cell line is not only susceptible to HCV infection but also supports its replication in vitro. Expression of HCV structural proteins can be detected in infected HepG2 cells. These cells are also capable of shedding viral particles into culture media which in turn become infectious to uninfected cells.

关键词： Hepatitis C virus In vitro propagation Genomic replication Gene expression HepG2 cells

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Axonal degeneration in the anterior insular cortex is associated with Alzheimer’s co-pathology in Parkinson’s disease and dementia with Lewy bodies

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Translational Neurodegeneration 2022年第1期11卷 129-147页

作者： yasmine Y.Fathy Laura E.Jonkman John J.Bol Evelien Timmermans Allert J.Jonker Annemieke J.m.Rozemuller Wilma D.J.van de Berg Amsterdam UmC Department of Anatomy and NeurosciencesSection Clinical Neuroanatomy and BiobankingAmsterdam NeuroscienceVrije University AmsterdamO|2 Life Sciences buildingDe Boelelaan 11081081 HZ Amster-damNetherlands Amsterdam Neuroscience Program NeurodegenerationAmsterdamthe Netherlands Amsterdam UmC Department of Pathol-ogyAmsterdam NeuroscienceVrije University AmsterdamDe BoelelaanAmsterdamNetherlands Department of Neurology Erasmus Medical CenterPostbus 20403000 CA RotterdamNetherlands.

Background:Axons,crucial for impulse transmission and cellular trafficking,are thought to be primary targets of neurodegeneration in Parkinson’s disease(PD)and dementia with Lewy bodies(DLB).Axonal degeneration occurs early,preceeding and exceeding neuronal loss,and contributes to the spread of pathology,yet is poorly described outside the nigrostriatal *** insula,a cortical brain hub,was recently discovered to be highly vulnerable to pathology and plays a role in cognitive deficits in PD and *** aim of this study was to evaluate morphological features as well as burden of proteinopathy and axonal degeneration in the anterior insular sub-regions in PD,PD with dementia(PDD),and ***:α-Synuclein,phosphorylated(p-)tau,and amyloid-βpathology load were evaluated in the anterior insular(agranular and dysgranular)subregions of post-mortem human brains(n=27).Axonal loss was evaluated using modified Bielschowsky silver staining and quantified using *** damage was comprehensively studied using immunofluorescent multi-labelling and 3D confocal laser-scanning ***:Compared to PD and PDD,DLB showed significantly higherα-synuclein and p-tau pathology load,argyrophilic grains,and more severe axonal loss,particularly in the anterior agranular ***,the dysgranular insula showed a significantly higher load of amyloid-βpathology and its axonal density correlated with cognitive performance.p-Tau contributed most to axonal loss in the DLB group,was highest in the anterior agranular insula and significantly correlated with CDR global scores for *** and myelin showed degenerative changes including swellings,demyelination,and detachment of the axon-myelin ***:Our results highlight the selective vulnerability of the anterior insular sub-regions to various converging pathologies,leading to impaired axonal integrity in PD,PDD and DLB,disrupting their functional properties and potentially con

关键词： α-Synuclein Insular subregions Axonal length density Alzheimer’s disease pathology Neurofilament myelin

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Frequency of Antibiotic Resistance of Escherichia coliand Klebsiella pneumoniaeby Production of TOHO-Type β-Lactamases at Saint Camille Hospital, Ouagadougou (Burkina Faso)

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Advances in microbiology 2021年第12期11卷 713-722页

作者： Serge Sougué Amana mètuor-Dabiré Djénéba Ouermi yasmine Rahimatou Wend-Kouni Tiemtoré Bénao Stéphanie Lynseh Carine Sita Théodora mahoukèdè Zohoncon yasmine Aminata Bangré Elie Kabré Jacques Simporé Department of Biochemistry and microbiology Faculty of Applied Science and Technology Biomolecular Research Centre (CERBA/LABIOGENE) UFR-SVT Joseph KI-ZERBO University Ouagadougou Burkina Faso National Public Health Laboratory of Ouagadougou Ouagadougou Burkina Faso University of Dedougou Dedougou Burkina Faso Saint Thomas Aquinas University (USTA) Saint Camille Hospital of Ouagadougou Ouagadougou Burkina Faso

Extended-spectrum β-lactamase (ESBL) appeared some years after the introduction in hospital environment of unhydrolysable or extended-spectrum cephalosporins. Several studies have been reported on the blaTEm, blaCTX-m and blaSHV genes in ESBL producing Enterobacteria, however, very few studies reported in the literature were related to blaCTX-m subgroup blaTOHO. TOHO enzymes were responsible for healthcare-associated infections in hospitals and in the community. In Burkina Faso, data related to these types of enzymes were scarce. The purpose of this study was to detect TOHO enzymes in Escherichia coli and Klebsiella pneumoniae in order to know the prevalence of infections related to bacterial resistance due to TOHO enzymes at Saint Camille Hospital of Ouagadougou (Burkina Faso). The study was conducted firstly by microbiological identification of ESBLs-producing by Escherichia coli and Klebsiella pneumoniae using API 20 E gallery;secondly the antibiogram was performed by the diffusion method and finally the molecular characterization was made by conventional PCR to search for the blaTOHO gene. The visualization of the specific bands was made using the ultraviolet lamp (Gene Flash) for the photography of the gels. Data were entered and analyzed using Excel 2013 and EPI Info version 6.0 software. A p-value Escherichia coli and 18 (46.2%) Klebsiella pneumoniae. molecular characterization showed the presence of the blaTOHO gene in 25 bacterial strains (64.1%). It was therefore established in this study the existence of blaTOHO gene at Saint Camille Hospital in Ouagadougou in Burkina Faso. Our study made it possible to know the distribution of the blaTOHO gene in Escherichia coli and Klebsiella pneumoniae.

关键词： Antibiotic Resistance Bacteria ESBL Genes TOHO

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