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Deciphering and engineering high-light tolerant cyanobacteria for efficient photosynthetic cell factories

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Chinese Journal of Chemical Engineering 2021年第2期34卷 82-91页

作者： yaru xie Lei Chen Tao Sun Weiwen Zhang Laboratory of Synthetic Microbiology School of Chemical Engineering&TechnologyTianjin UniversityTianjin 300072China Frontier Science Center for Synthetic Biology and Key Laboratory of Systems Bioengineering Ministry of Education of ChinaTianjin 300072China Collaborative Innovation Center of Chemical Science and Engineering Tianjin 300072China Center for Biosafety Research and Strategy Tianjin UniversityTianjin 300072China Law School Tianjin UniversityTianjin 300072China

Development and utilization of“liquid sunshine”could be one of key solutions to deal with the issues of fossil fuel depletion and increasing carbon *** are the only prokaryotes capable of performing oxygenic photosynthesis,and their activity accounts for~25%of the total carbon fixation on *** importantly,besides their traditional roles as primary producers,cyanobacteria could be modified as“photosynthetic cell factories”to produce renewable fuels and chemicals directly from CO_(2) driven by solar energy,with the aid of cutting-edging synthetic biology *** their large-scale biotechnological application in the future,many challenges still need to be properly addressed,among which is cyanobacterial cell factories inevitably suffer from high light(HL)stress during large-scale outdoor cultivation,resulting in photodamage and even cell death,limiting their *** this review,we critically summarized recent progress on deciphering molecular mechanisms to HL and developing HL-tolerant chassis in cyanobacteria,aiming at facilitating construction of HLresistant chassis and promote the future application of the large-scale outdoor cultivation of cyanobacterial cell ***,the future directions on cyanobacterial chassis engineering were discussed.

关键词： High-light Stress Chassis Cell factories Cyanobacteria

The Orientation Control Of The Tree-Shaped Strings Network 36

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The Orientation Control Of The Tree-Shaped Strings Network

Identification of genes involved in cadmium-ion tolerance in evolutionary Synechocystis *** 6803 tolerant to both cadmium and high light

第36届中国控制会议

作者： yaru xie Genqi Xu Department of Mathematics Tianjin University

ISBN: (纸本)9781538629185

In the present paper, we study the orientation control of tree-shaped strings network by the approach feedforward control. First, we design a new controller through the dynamics condition of objective network to stabilize the boundary node a1,a2, a3 and have no effect the other part of network. Furthermore, the convergence of network system continue to be discussed under some control strategy when one of the dynamics condition of the boundary w(1, t) is unknown and the original network is still proved to converge to the network of target structure. The method here is appropriate for more complex graph and mathematical model.

关键词： Orientation control Anti-disturbance Convergence of network Lyapunov method

来源： cnki会议评论

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Chinese Journal of Chemical Engineering 2024年第10期74卷 74-82页

作者： Jie Xiong Zhengxin Dong yaru xie Weiwen Zhang Tao Sun Lei Chen Laboratory of Synthetic Microbiology School of Chemical Engineering&TechnologyTianjin UniversityTianjin 300072China Frontier Science Center for Synthetic Biology&Key Laboratory of Systems Bioengineering Ministry of Education of ChinaTianjin 300072China Center for Biosafety Research and Strategy Tianjin UniversityTianjin 300072China

Photosynthetic cyanobacteria have shown great potential as“autotrophic cell factories”for the synthesis of fuels and ***,poor tolerance to various environmental stressors such as high light and heavy metals is an important factor limiting their economic *** numerous studies have focused on the tolerance mechanism of cyanobacteria to individual stressors,their response to simultaneous stresses remains to be *** investigate the mechanism of cross tolerance to heavymetal Cd^(2+) and high light,the model cyanobacterium Synechocystis *** 6803 tolerant to both Cd^(2+) and high light was obtained via about 800 days’cross-adaptive laboratory *** evolutionary strains capable of tolerating both 5.5 μmol·L^(-1) Cd^(2+) and 600 μmol·m^(-2)·s^(-1) high light were successfully obtained,achieving about 83%enhancement of Cd^(2+) tolerance compared with the parent *** different response of parent and evolutionary strains to Cd^(2+) was elucidated via ***,a total of 15 genes that were mutated during evolution were identified by whole-genome ***,by single-gene knockout and complementation analysis,four genes including ssl2615,sll1732,ssr1480,and sll1659 involved in the improvement of Cd^(2+) tolerance under high-light condition were successfully *** work explored the tolerance mechanism of Synechocystis *** 6803 to cadmium under high-light condition and provided valuable reference for deciphering multitolerance mechanism of cyanobacteria in the future.

关键词： Synechocystis Adaptive laboratory evolution Cross tolerance Cadmium-ion tolerance

Measuring bulk and surface acoustic modes in diamond by angle-resolved Brillouin spectroscopy

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Science China(Physics,Mechanics & Astronomy) 2021年第8期64卷 78-84页

作者： yaru xie ShuLiang Ren YuanFei Gao XueLu Liu PingHeng Tan Jun Zhang State Key Laboratory of Superlattices and Microstructures Institute of SemiconductorsChinese Academy of SciencesBeijing 100083China Center of Materials Science and Optoelectronics Engineering University of Chinese Academy of SciencesBeijing 100049China Beijing Academy of Quantum Information Science Beijing 100193China CAS Center of Excellence in Topological Quantum Computation University of Chinese Academy of SciencesBeijing 100049China

The acoustic modes of diamond are not only of profound significance for studying its thermal conductivity, mechanical properties, and optical properties, but also play a definite role in the performance of high-frequency and high-power acoustic wave devices. Here, we report on the bulk acoustic waves(BAWs) and surface acoustic waves(SAWs) of single-crystal diamond using angle-resolved Brillouin light scattering(BLS) spectroscopy. We identify two high-speed surface skimming bulk waves(SSBW) with acoustic velocities of 1.277×10^(6) and 1.727×10^(6) cm/s, respectively. Furthermore, we obtain the relationship between the velocity of arbitrary BAWs and that of BAWs propagating along the high-symmetric axis at different incident angles. In the community of diamond-based acoustic studies, our results may provide a valuable reference for fundamental research and device engineering.

关键词： diamond acoustic waves angle-resolved Brillouin spectroscopy

Stiffness-tunable biomaterials provide a good extracellular matrix environment for axon growth and regeneration

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Neural Regeneration Research 2025年第5期20卷 1364-1376页

作者： Ronglin Han Lanxin Luo Caiyan Wei yaru Qiao Jiming xie Xianchao Pan Juan Xing Department of Pathophysiology School of Basic Medical SciencesSouthwest Medical UniversityLuzhouSichuan ProvinceChina Department of Medicinal Chemistry School of PharmacySouthwest Medical UniversityLuzhouSichuan ProvinceChina

Neuronal growth, extension, branching, and formation of neural networks are markedly influenced by the extracellular matrix—a complex network composed of proteins and carbohydrates secreted by cells. In addition to providing physical support for cells, the extracellular matrix also conveys critical mechanical stiffness cues. During the development of the nervous system, extracellular matrix stiffness plays a central role in guiding neuronal growth, particularly in the context of axonal extension, which is crucial for the formation of neural networks. In neural tissue engineering, manipulation of biomaterial stiffness is a promising strategy to provide a permissive environment for the repair and regeneration of injured nervous tissue. Recent research has fine-tuned synthetic biomaterials to fabricate scaffolds that closely replicate the stiffness profiles observed in the nervous system. In this review, we highlight the molecular mechanisms by which extracellular matrix stiffness regulates axonal growth and regeneration. We highlight the progress made in the development of stiffness-tunable biomaterials to emulate in vivo extracellular matrix environments, with an emphasis on their application in neural repair and regeneration, along with a discussion of the current limitations and future prospects. The exploration and optimization of the stiffness-tunable biomaterials has the potential to markedly advance the development of neural tissue engineering.

关键词： alginate axon growth biomaterials extracellular matrix neural repair neurons neuroregeneration polyacrylamide polydimethylsiloxane stiffness

Euler-Bernoulli梁系统的局部分数阶反馈镇定

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天津科技大学学报 2017年第2期32卷 73-78页

作者：王雷解雅茹天津理工大学理学院天津大学理学院

对Euler-Bernoulli梁的稳定性进行研究,首先在系统内部设计了局部分数阶反馈控制器,通过非线性算子半群理论得到了闭环系统的适应性;然后通过选取适当的乘子,借用乘子技巧得到了系统的指数稳定性与多项式稳定性,并给出了系统能量的衰减... 详细信息

对Euler-Bernoulli梁的稳定性进行研究,首先在系统内部设计了局部分数阶反馈控制器,通过非线性算子半群理论得到了闭环系统的适应性;然后通过选取适当的乘子,借用乘子技巧得到了系统的指数稳定性与多项式稳定性,并给出了系统能量的衰减性估计;最后通过数值模拟验证了局部分数阶反馈控制器的有效性.

关键词： Euler-Bernoulli梁分数阶局部反馈控制半群理论

高强度超声作用下鲢鱼肌球蛋白的结构及流变学特性变化

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食品科学 2019年第5期40卷 77-84页

作者：谢亚如刘庆熊善柏尤娟刘茹华中农业大学食品科学技术学院湖北武汉430070 国家大宗淡水鱼加工技术研发分中心(武汉) 湖北武汉430070

以鲢鱼为原料提取纯化肌球蛋白,研究不同高强度超声处理条件(100、150、200、250 W分别超声3、6、9、12 min)下肌球蛋白链构象、二级结构及流变学特性的变化。结果表明:超声处理会降低肌球蛋白的低温自组装程度,使样品的零剪切黏度逐渐... 详细信息

以鲢鱼为原料提取纯化肌球蛋白,研究不同高强度超声处理条件(100、150、200、250 W分别超声3、6、9、12 min)下肌球蛋白链构象、二级结构及流变学特性的变化。结果表明:超声处理会降低肌球蛋白的低温自组装程度,使样品的零剪切黏度逐渐降低、流动性增大,并诱导肌球蛋白α-螺旋向较为松散的β-折叠和无规卷曲结构转变,促使包埋在分子内部的活性基团暴露出来。该结构变化有利于升温过程中分子间的交联,并降低凝胶化温度,适当的超声条件(100、150 W处理12 min)可提高肌球蛋白的凝胶形成能力,而超声功率过大(200、250 W处理12 min)则会降低其凝胶形成能力,选择适当的超声条件对鱼糜凝胶加工十分重要。

关键词：肌球蛋白高强度超声结构流变学特性鲢鱼

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高等教育大众化背景下大学文化建设若干思考

黑河学院学报 2017年第10期8卷 84-85页

作者：谢雅儒福州外语外贸学院福建福州350202

大学文化是学校的灵魂所在,也是大学的核心竞争力,是大学所有师生通过长期的实践并上升为价值观念形成的,承载着培养后代优秀大学人的梦想。在当今新中国文化建设的大背景下,大学文化建设已经越来越重要。这种大背景需要我们需要有更多... 详细信息

大学文化是学校的灵魂所在,也是大学的核心竞争力,是大学所有师生通过长期的实践并上升为价值观念形成的,承载着培养后代优秀大学人的梦想。在当今新中国文化建设的大背景下,大学文化建设已经越来越重要。这种大背景需要我们需要有更多的思考,重视大学文化、发展大学文化。在新的背景下,大学制度的创新,要充分发挥环境文化的作用。

关键词：大学文化文化建设高等教育

同源细胞膜包载多西他赛长循环脂质体的制备及体外评价

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中国医药工业杂志 2021年第10期52卷 1329-1336页

作者：解雅茹王冠茹孙考祥烟台大学药学院山东烟台264005 中国科学院上海药物研究所药物制剂研究中心上海201203

以氢化大豆磷脂酰胆碱、二硬脂酰磷脂酰乙醇胺-聚乙二醇2000及胆固醇为载体,采用薄膜分散法制备了多西他赛长循环脂质体(lip-DTX)。在此基础上,采用共挤出法制备小鼠乳腺癌(4T1)细胞膜包裹的多西他赛长循环脂质体(4T1/lip-DTX)。透射电... 详细信息

以氢化大豆磷脂酰胆碱、二硬脂酰磷脂酰乙醇胺-聚乙二醇2000及胆固醇为载体,采用薄膜分散法制备了多西他赛长循环脂质体(lip-DTX)。在此基础上,采用共挤出法制备小鼠乳腺癌(4T1)细胞膜包裹的多西他赛长循环脂质体(4T1/lip-DTX)。透射电镜观察到所制得的lip-DTX和4T1/lip-DTX形态圆整;粒度分析仪测得其粒径为(149.6±0.1)和(126.7±0.6)nm,ζ电位为(–40.5±2.1)和(–6.3±1.2)mV;超滤法测得包封率为(72.4±0.3)%和(74.1±0.2)%,载药量为(3.0±0.1)%和(2.5±0.5)%。二者在4℃条件下4 d内粒径和包封率均保持稳定。体外释放试验结果显示,lip-DTX释放缓慢,12 h时累积释放率为(69.5±0.4)%,而4T1/lip-DTX释放更缓慢,8 h累积释放率为(22.2±0.3)%。细胞摄取试验显示,小鼠单核巨噬(RAW 264.7)细胞对lip-DTX的摄取是4T1/lip-DTX的1.4倍(P<0.01),4T1细胞对4T1/lip-DTX的摄取是lip-DTX的1.5倍(P<0.001),提示4T1/lip-DTX具有长循环和靶向持性。4T1细胞毒性试验显示,lip-DTX及4T1/lip-DTX对4T1细胞的IC50值为7.6和1.5μg/ml,提示4T1/lip-DTX能增加药物的抗肿瘤效果。

关键词：同源细胞膜多西他赛长循环脂质体仿生制剂体外评价