检索结果-南通市图书馆

我国图书馆事业政策的多源流分析:变迁进程与变迁因素

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图书馆建设 2024年第2期 41-50页

作者：付亚萍杨峰四川大学公共管理学院四川大学公共管理学院信息资源管理系

准确理解图书馆事业的政策变迁逻辑是推动图书馆高质量发展的重要前提。基于对91项图书馆事业发展政策的资料分析可知,新中国成立以来我国图书馆事业政策变迁进程划分为星星之火的站起来阶段(1949—1978年)、多点发力的富起来阶段(1979... 详细信息

准确理解图书馆事业的政策变迁逻辑是推动图书馆高质量发展的重要前提。基于对91项图书馆事业发展政策的资料分析可知,新中国成立以来我国图书馆事业政策变迁进程划分为星星之火的站起来阶段(1949—1978年)、多点发力的富起来阶段(1979—2012年)和高位推进的强起来阶段(2013年至今)三个阶段。从多源流理论视角就图书馆事业政策变迁因素进行解构,可以得出各阶段的发展瓶颈构成问题流,多方建言构成政策流,高层重视构成政治流,多源耦合开启政策之窗并推动图书馆事业政策变迁。

关键词：图书馆政策图书馆事业政策变迁多源流理论

Targeted mutagenesis in rice using CRISPR-Cpf1 system

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Journal of Genetics and Genomics 2017年第1期44卷 71-73页

作者： Xixun Hu Chun Wang Qing Liu yaping fu Kejian Wang State Key Laboratory of Rice Biology China National Rice Research InstituteChinese Academy of Agricultural Sciences Hangzhou 310006 China

Cpf1 is a class 2/type V CRISPR effector that has been recently harnessed for genome editing （Zetsche et al., 2015; Hut et al., 2016; Kim et al., 2016）. Cpff recognizes thymidine-rich sequence as the protospacer-adjacent motif （PAM） at the 5＇ end of target sequences. In addition, Cpfl requires only a single shorter crRNA and cleaves DNA in a staggered fashion with 5＇ overhangs （Zetsche et al., 2015）.

关键词： Targeted mutagenesis in rice using CRISPR-Cpf1 system tRNA

A Simple CRISPR/Cas9 System for Multiplex Genome Editing in Rice

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Journal of Genetics and Genomics 2015年第12期42卷 703-706页

作者： Chun Wang Lan Shen yaping fu Changjie Yan Kejian Wang State Key Laboratory of Rice Biology China National Rice Research Institute Chinese Academy of Agricultural Sciences Key Laboratory of Plant functional Genomics Ministry of EducationYangzhou University

Generating mutants bearing multiple gene modifications is essential for determining the functions of gene family members with redundant functions, or for analyzing epistatic re- lationships in genetic pathways. Using conventional methods, mutants with multiple gene mutations are generated by several rounds of intercrossing plants carrying a single mutation and identification of the offspring. This process is both timeconsuming and labor-intensive. Moreover, if the genes of interest are closely linked, multiple mutations can not be generated （Wijnker and de Jong, 2008）.

关键词： A Simple CRISPR/Cas9 System for Multiplex Genome Editing in Rice PDS Kpn RNA gene PCR

Distributed Scheduling Problems in Intelligent Manufacturing Systems

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Tsinghua Science and Technology 2021年第5期26卷 625-645页

作者： yaping fu Yushuang Hou Zifan Wang Xinwei Wu Kaizhou Gao Ling Wang the College of Business Qingdao UniversityQingdao 266071China the Institute of Systems Engineering Macao University of Science and TechnologyMacao 999078China the Department of Automation Tsinghua UniversityBeijing 100084China

Currently,manufacturing enterprises face increasingly fierce market competition due to the various demands of customers and the rapid development of economic globalization.Hence,they have to extend their production mode into distributed environments and establish multiple factories in various geographical locations.Nowadays,distributed manufacturing systems have been widely adopted in industrial production processes.In recent years,many studies have been done on the modeling and optimization of distributed scheduling problems.This work provides a literature review on distributed scheduling problems in intelligent manufacturing systems.By summarizing and evaluating existing studies on distributed scheduling problems,we analyze the achievements and current research status in this field and discuss ongoing studies.Insights regarding prior works are discussed to uncover future research directions,particularly swarm intelligence and evolutionary algorithms,which are used for managing distributed scheduling problems in manufacturing systems.This work focuses on journal papers discovered using Google Scholar.After reviewing the papers,in this work,we discuss the research trends of distributed scheduling problems and point out some directions for future studies.

关键词： distributed manufacturing systems distributed scheduling problems modeling and optimization intel igent optimization methods

改进蝴蝶优化算法在护理人员路径规划与调度中的应用

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控制工程 2024年第2期31卷 281-287页

作者：浦徐进李丽超付亚平江南大学商学院江苏无锡214122 青岛大学商学院山东青岛266071

针对养老资源利用不充分的问题,提出一种同时考虑成本和顾客满意度的医疗护理人员的路径规划与调度,基于服务成本、顾客满意度两个方面建立多目标多家庭护理企业的路径优化模型。根据建立的优化模型,提出一种改进后的蝴蝶优化算法进行求... 详细信息

针对养老资源利用不充分的问题,提出一种同时考虑成本和顾客满意度的医疗护理人员的路径规划与调度,基于服务成本、顾客满意度两个方面建立多目标多家庭护理企业的路径优化模型。根据建立的优化模型,提出一种改进后的蝴蝶优化算法进行求解,将快速非支配排序与蝴蝶优化算法相结合,从而提高对最优解集的搜索效率,使得“蝴蝶”能更好地追随最优“蝴蝶群”,提高收敛速度和保持解的多样性。实验结果表明,所提出的优化模型和算法能够兼顾成本和顾客满意度两个目标,得出最优解集。

关键词：家庭医疗路径规划蝴蝶优化算法

A Pid3 allele from rice cultivar Gumei2 confers resistance to Magnaporthe oryzae

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Journal of Genetics and Genomics 2011年第5期38卷 209-216页

作者： Jie Chen Yongfeng Shi Wenzheng Liu Rongyao Chai yaping fu Jieyun Zhuang Jianli Wu State Key Laboratory of Rice Biology China National Rice Research Institute Hangzhou 310006 China Zhejiang Academy of Agricultural Sciences Hangzhou 310028 China

Rice blast, caused by Magnaporthe oryzae, is one of the most devastating diseases. Using map-based strategy and in silico approach we isolated a new rice （Oryza sativa L.） blast resistance allele of Pid3, designated Pi25, from a stable blast resistance cultivar Gumei2. Overexpression analysis and complementation test showed that Pi25 conferred blast resistance to M. oryzae isolate js001-20. Sequence analysis showed that Pi25 was an intronless gene of 2772 nucleotides with single nucleotide substitution in comparison to Pid3 at the nucleotide position 459 and predicatively encoded a typical coiled coil-nucleotide binding site-leucine rich repeat （CC-NBS-LRR） protein of 924 amino acid residuals with 100% identity to Pid3 putative protein. The susceptible allele pi25 in Nipponbare contained a nonsense mutation at the nucleotide position 2209 resulting in a truncated protein with 736 amino acid residuals. In addition, 14 nucleotide substitutions resulting in 10 amino acid substitutions were identified between Pi25 and pi25 upstream the premature stop codon in the susceptible allele. Although the mechanism of Pi25/Pid3-mediated resistance needs to be further investigated, the isolation of the allele would facilitate the utilization of Pi25/Pid3 in rice blast resistance breeding program via transgenic approach and marker assisted selection.

关键词： Oryza sativa Magnaporthe oryzae Blast resistance gene NBS-LRR

Expanding the Range of CRISPR/Cas9 Genome Editing in Rice

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Molecular Plant 2016年第6期9卷 943-945页

作者： Xixun Hu Chun Wang yaping fu Qing Liu Xiaozhen Jiao Kejian Wang State Key Laboratory of Rice Biology China National Rice Research Institute Chinese Academy of Agricultural Sciences Hangzhou 310006 China These authors contributed equally to this article.

Dear Editor The CRISPR/Cas9 system has emerged as a versatile molecular tool for genome editing in various organisms, including plants. In this system, the specificity of Cas9-directed DNA cleavage strictly requires the presence of a chimeric single guide RNA （sgRNA） and a short trinucleotide protospacer adjacent motif （PAM） in the genome （Anders et al., 2014; Sternberg et al., 2014）. To date, the Cas9 used in plants has only been shown to recognize PAM sequences in the canonical form NGG （Li et al., 2013; Miao et al., 2013; Shan et al., 2013; Ma et al., 2015b）. As such, the range of sequences for genome editing in plants is limited to sites containing an NGG motif. Many attempts have been made to overcome this constraint, and recent work has revealed that Cas9 can be modified to recognize alternative PAM sequences in zebrafish and human cells （Kleinstiver et al., 2015）. However, the targeting range limitations of the CRISPR/ Cas9 systems in plants have yet to be resolved. The widely cultivated plant rice is not only an important food crop but also a model crop plant because of its relatively small genome and relative ease of transformation. To expand the range of genome editing in rice, we generated two Cas9 variants as reported （Kleinstiver et al., 2015） and investigated their genome editing performance in rice. We demonstrate that Cas9 can be engineered to target sites containing alternative non-canonical PAMs in rice, which significantly broadens the range of genorne editing.

关键词： genome presence trinucleotide

大气污染物质量浓度对黄土高原降水云物理影响研究

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环境科学与管理 2024年第5期49卷 40-45页

作者：付亚平李芳山西省大气探测技术保障中心山西太原030002 山西省气象学会山西太原030002

针对黄土高原实际降水展开研究,探究大气污染物质量浓度对黄土高原降水情况的影响程度。将黄土高原1990年-2019年共30年的气象数据资料作为实验数据,通过计算提取出黄土高原降水的分布特征,根据实际的大气污染物质量浓度,设定不同的大... 详细信息

针对黄土高原实际降水展开研究,探究大气污染物质量浓度对黄土高原降水情况的影响程度。将黄土高原1990年-2019年共30年的气象数据资料作为实验数据,通过计算提取出黄土高原降水的分布特征,根据实际的大气污染物质量浓度,设定不同的大气污染物质量浓度环境,再计算大气污染物质量浓度与黄土高原降水情况的相关度,并对其进行分析。实验结果显示,大气污染物质量浓度越低,黄土高原的降水时间、降水强度和降水次数越高。黄土高原的降水情况会受到多种因素的影响,其中,大气污染物质量浓度对黄土高原降水情况的物理影响较大。

关键词：大气污染物质量浓度黄土高原高原降水物理影响实验分析

The Rice＇NUTRITION RESPONSE AND ROOT GROWTH＇（NRR） Gene Regulates Heading Date

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Molecular Plant 2013年第2期6卷 585-588页

作者： Yuman Zhang Ge Zhang Na Xiao Lina Wang yaping fu Zongxiu Sun Rongxiang Fang Xiaoying Chen State Key Laboratory of Plant Genomics Institute of Microbiology Chinese Academy of Sciences Beijing 100101 China National Plant Gene Research Center Beijing 100101 China Graduate School of the Chinese Academy of Sciences Beijing 100039 China Department of Ornamental Horticulture and Landscape Architecture China Agricultural University Beijing 100193 China State Key Laboratory of Rice Biology National Rice Research Institute Hangzhou 310006 China

Dear Editor, As one of the most important developmental traits, flow-ering time has been extensively investigated in the long-day plant Arabidopsis. Multiple factors are involved, including photoperiod and/or temperature （Amasino, 2010）. Flowering locus T （FT） is known to be an important integrator of dif-ferent flowering pathways （Taoka et al., 2011）. Genes of the CONSTANS （CO） family also play key roles in regulating flow-ering time. The CO protein consists of a zinc finger and a CCT domain shared by a group of plant-specific transcription fac-tors that regulate photoperiodic flowering response and cir-cadian rhythms in Arabidopsis.

关键词： ARABIDOPSIS TRANSITION EXPRESSION DEFICIENT HD3A EHD1