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Asian Pacific Journal of Tropical Medicine 2019年第10期12卷 472-478页

作者： May-Ling yap Zamberi Sekawi Hui-yee Chee Han Kiat Alan Ong Vasantha Kumari Neela Department of Medical Microbiology and Parasitology Faculty of Medicine and Health SciencesUniversiti Putra Malaysia43400 SerdangSelangorMalaysia Department of Pre-Clinical Sciences Faculty of Medicine and Health SciencesUniversiti Tunku Abdul RahmanBandar Sungai Long43000 KajangSelangorMalaysia

Objective:To compare the efficiency of routine diagnostic PCR assays in detecting pathogenic Leptospira isolated from water and ***:Seven routine assays targeting six genes(lip L32,fla B,gyr B,lfb1,sec y and lig B)were evaluated and compared on the cultures of two groups of pathogenic Leptospira from different *** group included 19 described reference strains recovered from infected human or animals,and another group included 22 environmental isolates from recreational and residential sites in *** latter have been confirmed for presence of pathogenic Leptospira *** positivity or detection sensitivity of each assay was determined and compared between the two ***:Validation on reference strains showed 100.0%PCR sensitivity for all assays except lig B-PCR(95.0%)that failed to amplify Leptospira interrogans serovar *** marked contrast,there was a notable decline in sensitivity in the environmental isolates(lip L32-PCR,95.5%;fla B-PCR,90.9%;gyr B-PCR,77.3%;lfb1-PCR,59.1%;sec y-PCRs,40.9%g1/g2-PCR,36.4%;lig B-PCR,13.6%),implying a large genetic distance between the two groups,as well as nucleotide polymorphism among environmental ***:High proportion of false-negative PCR results suggests a need of prudent selection of primers in detecting environmental pathogenic *** findings offer valuable insights on the extensive biodiversity of genus Leptospira and its impact on the efficacy and development of molecular detection tool.

关键词： Leptospira Pathogenic species Environmental samples PCR Sensitivity

高危心肌梗死患者发生心律失常性和非心律失常性死亡风险的时间变化趋势:对多中心试验的综合分析

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世界核心医学期刊文摘(心脏病学分册) 2006年第2期 46-47页

作者： yap y. g. 杜媛 Department of Cardiological Sciences St. george’ s Hospital Medical School Cranmer Terrace London SW17 0RE United Kingdom

Aims: An understanding of the temporal trends on the risks of arrhythmic death(AD) vs. non-arrhythmic deaths(NAD) after myocardial infarction(MI) is crucial in deciding the optimal timing for risk stratification and treatment window for prophylactic antiarrhythmic treatment. However, contemporary data on such information is lacking. Methods and results: Individual patient data were pooled from th placebo arms of EMIAT, CAMIAT, SWORD, TRACE, and DIAMOND-MI who had a recent MI and left ventricular ejection fraction(LVEF)gated for all studies from day 45 after acute myocardial infarction(AMI) to account for different recruitment periods between trials, and then from the onset of MI for TRACE and DIAMOND-MI that recruited patients within 2 weeks after MI. In total, 3104 patients(median age 65, range: 23-92; 2471 males)were pooled from all five studies, with a total of 487 deaths at 2-year follow-up; 220 deaths were ADs and 172 were NADs. The risks of both AD and NAD were highest in the first 6 months but the risk of AD was consistently higher than that of NAD throughout the 2-year period[rate of death/100 person-year at risk(AD/NAD): 8.09/6.07(45 days to 6 months), 4.07/3.35(>6-12 months), 4.34/3.60(>12-18 months), 3.76/2.77(>18-24 months)]. There were significant interactions between the temporal trends of mortalities and gender(P=0.03) and history of hypertension(P=0.04). A similar trend was observed when mortality was measured from time of onset of MI from the combined TRACE and DIAMOND-MI dataset. Conclusion: Our study provided the first contemporary evidence that in high-risk post-MI patients with LVEFgher than that of NAD for up to 2 years although in female patients, they became increasingly more likely to die from NAD after 6 months. Therefore, risk stratification of post-MI patient at high risk of AD remains a worthwhile exercise. Howeve

关键词：心律失常患者危险分层 AD

Sonication and grinding pre-treatments on gelidium amansii seaweed for the extraction and characterization of Agarose

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Frontiers of Environmental Science & Engineering 2018年第4期12卷 17-23页

作者： Kit Wayne Chew Pau Loke Show yee Jiun yap Joon Ching Juan Siew Moi Phang Tau Chuan Ling Jo-Shu Chang Department of Chemical and Environmental Engineering Faculty of Engineering University of Nottingham Malaysia Campus Jalan Broga 43500 Semenyih Selangor Darul Ehsan Malaysia Bioseparation Research group Faculty of Engineering University of Nottingham Malaysia Campus Jalan Broga 43500 Semenyih Selangor Darul Ehsan Malaysia Department of Applied Mathematics Faculty of Engineering University of Nottingham Malaysia Campus Jalan Broga 43500 Semenyih Selangor Darul Ehsan Malaysia Nanotechnology & Catalysis Research Centre (NANOCAT) Institute of Postgraduate Studies University of Malaya 50603 Kuala Lumpur Malaysia Institute of Ocean and Earth Sciences University of Malaya 50603 Kuala Lumpur Malaysia Institute of Biological Sciences University of Malaya 50603 Kuala Lumpur Malaysia Department of Chemical Engineering National Cheng Kung University Tainan 701 Taiwan China

Various prctreatments methods including sonication and grinding were perfonned on red seaweed gelidium amansii for the subsequent extraction ofagarose. The agarose products are usually extracted from agar powder products from seaweeds. In this study, the agarose was extracted using a direct polyethylene glycol （PEg） method without the need to first process the agar from seaweed. The agar extract was frozen then thawed and mixed directly with PEg solution to precipitate the agarose. The quality of agarose obtained was evaluated through physico-chemical properties analysis which includes spectral technique （FTIR）, melting and boiling point, gel strength and sulfate content. These properties were compared with a non-pretreated sample and it was found that the addition of pretreatment steps improved the quality of agarosc but gave a slightly lower yield. The gel strength of pretreated samples was much higher and the sulfate content was lower compared to non-pretreated samples. The best pretreatment method was sonication which gave gel strength of 742 gcm - and sulfate content of 0.63%. The extraction of agarose can be further improved with the use of different neutralizing agents. Pretreating the seaweed shows potential in improving the quality of agarosc from seaweed and can be applied for thture extraction of the agarose.

关键词： Agarosege lidium Pretreatment Seaweed Sonication

graphene-based Q-switched pulsed fiber laser in a linear configuration

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Chinese Optics Letters 2012年第4期10卷 36-39页

作者： y.K.yap Richard M.De La Rue C.H.Pua S.W.Harun H.Ahmad Photonics Research Centre Department of PhysicsFaculty of ScienceUniversity of MalayaKuala LumpurMalaysia Department of Electrical Engineering Faculty of EngineeringUniversity of MalayaKuala LumpurMalaysia

A pulsed laser system is realized with graphene employed as a *** graphene is exfoliated from its solution using an optical deposition and the optical tweezer effect.A fiber ferrule that already has the graphene deposited on it is inserted into an erbium-ytterbium laser（EyL）system with linear cavity *** successfully demonstrate a pulsed EyL with a pulse duration of approximately 5.9μs and a repetition rate of 20.0 kHz.

关键词： mode FWHM kHz Figure

Effects of Milling Duration on Malaysian Dolomite to Enhance Hydrogen Production via gasification of Oil Palm Fronds

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Effects of Milling Duration on Malaysian Dolomite to Enhance...

2012 4th International Conference on Chemical,Biological and Environmental Engineering

作者： A. R. Nur-Faizal M. Z. Hussein y. H. Taufiq-yap Center of Excellence for Catalysis Science and Technology Faculty of ScienceUniversiti Putra Malaysia43400 UPM SerdangSelangorMalaysia Department of Chemistry Faculty of ScienceUniversiti Putra Malaysia43400 UPM SerdangSelangorMalaysia

The purpose of this work is to investigate the effect of mechanochemical treatment on Malaysian dolomite towards the enhancement of hydrogen production from gasification reaction of oil palm fronds (OPF).To accomplish the objective,Malaysian dolomite was subjected to high energy planetary ball mill for 1,2 and 4 h in *** milling process reduced the particle size of the catalysts and consequently increased their surface *** catalytic activity was investigated through temperature programmed gasification (TPg).The catalyst premixed with the biomass in a ratio of 1:1 and undergone TPg under partial oxygen environment (5% O2 in He) tested from ambient temperature up to 900oC with heating rate 10oC/*** the gases produced were detected by online mass *** milled catalysts prominently showed the increasing of H2 production together with the reduction of CO2 produced due to the dual function of catalysts which acted as tar reduction and CO2 sorbent.

关键词： Dolomite,Hydrogen production,CaO,Oil palm fronds,Mechanochemical treatment.

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荧光血管造影后广泛上肢静脉栓塞

世界核心医学期刊文摘（眼科学分册） 2005年第9期1卷 23-24页

作者： yap E.-y. Eong K.-g.A. 张鹏

PURPOSE: To report a case of extensive cephalic and basilic venous thrombosis after fluorescein angiography. DESIgN: Interventional case report. METHODS: A 38-year-old Chinese male presented with blurring of vision in his left eye. Clinical examination revealed central serous retinopathy, and a routine fluorescein angiography was performed. RESULTS: He developed inflammation over the right arm over 4 days. Duplex scan disclosed extensive superficial venous thrombosis of the right upper limb with occlusion of the basilic vein and cephalic vein in the proximal right upper arm to the level of the midarm. He was treated medically but was subsequently lost to follow-up. CONCLUSIONS: Extensive upper-extremity venous thrombosis is infrequent after single-shot intravenous cannulation. Venous thrombosis occurring after fluorescein angiography has not been reported up to this time. It is possible that proper arm positioning during the procedure may reduce this risk, and ophthalmologists should be vigilant of this potentially unusual complication.

关键词：荧光血管造影静脉栓塞中心性静脉血栓形成左眼视物模糊干预性眼科医生头静脉栓塞病静脉管

血脂水平在不同CKD分期患者中与心血管事件和全因死亡预后关系研究

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新医学 2020年第6期51卷 439-444页

作者：胡文涛李少敏刘佩佳 Desmond y.H.yap 刘迅中山大学附属第三医院肾内科广州510630

目的研究HDL-C、LDL-C、甘油三酯、载脂蛋白A(APOA)、APOB在不同慢性肾脏病(CKD)分期患者中与心血管事件以及全因死亡的关系。方法收集CKD患者数据并进行汇总及分期,随访结局(心血管事件和全因死亡),采用Cox回归分析HDL-C、LDL-C、甘油... 详细信息

目的研究HDL-C、LDL-C、甘油三酯、载脂蛋白A(APOA)、APOB在不同慢性肾脏病(CKD)分期患者中与心血管事件以及全因死亡的关系。方法收集CKD患者数据并进行汇总及分期,随访结局(心血管事件和全因死亡),采用Cox回归分析HDL-C、LDL-C、甘油三酯、APOA、APOB与各CKD分期患者心血管事件和全因死亡之间的关系。结果经校正年龄、性别、BMI、糖尿病与否、现吸烟与否、现饮酒与否、血清尿酸、血清前白蛋白、血钾、血红蛋白后,LDL-C在CKD1、2、4、5期患者中均为心血管事件的危险因素(P均0.05);HDL-C在CKD1~4期患者中均为全因死亡的危险因素(P均0.05)。结论甘油三酯和APOB在CKD患者心血管事件和全因死亡中可能有重要预测作用,需要高度关注,HDL-C在全因死亡预测中起到一定作用,LDL-C在心血管事件预测中起到一定作用,APOA则在心血管事件和全因死亡中均无预测作用。

关键词：高密度脂蛋白胆固醇低密度脂蛋白胆固醇甘油三酯载脂蛋白A 载脂蛋白B 慢性肾脏病心血管事件全因死亡

SyNTHESIS,CHARACTERIZATION AND CATALyTIC PERFORMANCE OF Γ-AL2O3 SUPPORTED VANADyL PyROPHOSPHATE CATALySTS

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SYNTHESIS,CHARACTERIZATION AND CATALYTIC PERFORMANCE OF Γ-A...

独活药材显微鉴定的一个新的显著特征(英文)

40th IUPAC Congress

作者： y.H.Taufiq-yap L.K.Leong M.Z.Hussein and R.Irmawati Department of Chemistry Universiti Putra Malaysia 43400 UPM Serdang Selangor Malaysia

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中药材 2008年第12期31卷 1796-1802页

作者：汤俊冯奕斌童瑶叶敏玲文英强香港大学中医药学院武汉大学药学院湖北武汉430072 香港大学药理学系

目的:为了更有效地鉴定中药独活并作为该药材标准化的基础,我们对其详细的形态和显微特征进行了深入的研究。方法:从独活的道地产区收集药材样本,并采用传统的生药学鉴定手段对其形态和显微特征进行研究并与文献进行了比较。结果:发现... 详细信息

目的:为了更有效地鉴定中药独活并作为该药材标准化的基础,我们对其详细的形态和显微特征进行了深入的研究。方法:从独活的道地产区收集药材样本,并采用传统的生药学鉴定手段对其形态和显微特征进行研究并与文献进行了比较。结果:发现了一种明显的但一直被忽视的粉末新特征,即具有交错纹理的纺锤形薄壁细胞,可作为一个重要的鉴定独活药材的显微特征。此外,本文对其形态及其它显微特征及文献的异同分别进行了描述和探讨。结论:这些结果为完善独活的药材鉴定提供了重要的科学依据。

关键词：独活显微鉴定具有交错纹理的纺锤形薄壁细胞