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检索条件"作者=Yaozhou Zhang~* Qingyou Xia~ Jie Xu~* Lingying Kong~* Jian Chen~* Zuoming Nie~* Dan Wang~* Jianqing Chen~* Qingliang Zheng~* Qing Chen~* Xiaoyuan Ren~* Jiang Wang~* Zhengbing Lv~* Caiying Jiang~* Lili Liu~* Qing Sheng Yongfeng Jin~ Xiangfu Wu~* ~*The key laboratory of Bioreactor and Biopharmacy of zhejiang province"

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Proteomic identification and function analysis of the silkworm,Bombyx mori

Proteomic identification and function analysis of the silkwo...

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中国生物化学与分子生物学会农业生物化学与分子生物学分会第八次学术研讨会

作者： yaozhou zhang~* qingyou xia~+ jie xu~* lingying kong~* jian chen~* zuoming nie~* dan wang~* jianqing chen~* qingliang zheng~* qing chen~* xiaoyuan ren~* jiang wang~* zhengbing lv~* caiying jiang~* lili liu~* qing sheng yongfeng jin~(**) xiangfu wu~* ~*The key laboratory of bioreactor and biopharmacy of zhejiang province Institute of Biochemistry,zhejiang Sci-Tech University,Hangzhou 310018,China ~+The key Sericultural laboratory of Agricultural Ministry The key laboratory for Sericultural Sciences and Genomics of the Ministry of Education,College of Biotechnology,Southwest University,Beibei,Chongqing 400715,China ~(**)College of Life Sciences,zhejiang University,Hangzhou 310029,China

Mass spectrometry (MS) is an effective method for large-scale proteomic identification and verification of protein-encoding genes present in a *** this study,peptide sequences identified by MS were matched with those from an ORF library of the silkworm (Bombyx mori) genome and a cDNA library to validate putative *** databases were *** first was based on a 6x draft sequence of the silkworm genome and contained 14,632 putative *** second was based on a *** pupal cDNA library that had 3,187 putative proteins,each composed of at least 30 amino acid *** this study,81,000 peptide sequences that had a threshold score of 60% were generated by MS/MS analysis,and 55,400 were chosen for sequence *** searching the two databases,6,649 and 250 protein were matches,and these accounted for approximately 45.4% and 7.8% *** analysis and cataloging of the identified proteins,which were carried out by using several bioinformatic tools,suggested that the matches included proteins with predicted transmembrane domains (TMDs;1,393) and preproteins with a signal peptide (SP,976).These results provide a fundamental understanding of gene expression and function analysis of silkworm proteins.

关键词： Bombyx mori pupae protein identification function analysis ORF genome proteomics

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