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Chlamydia trachomatis infection in the genital tractis associated with inflammation and hypospermia inthe infertile male of China

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Asian Journal of Andrology 2022年第1期24卷 56-61页

作者： Hua Zhou Shunhong Wu Xiaohua Tang Guanqing Zhou Jingru Yuan Qing Li yaoyong chen Xia Xu Xiaofang Sun Detu Zhu Yumei Luo Department of Obstetrics and Gynecology Key Laboratory for Major Obstetric Diseases of Guangdong ProvinceThe Third Affiliated Hospital of Guangzhou Medical UniversityGuangzhou 510150China Key Laboratory of Reproduction and Genetics of Guangdong Higher Education Institutes The Third Affiliated Hospital of Guangzhou Medical UniversityGuangzhou 510150China Kingmed School of Laboratory Medicine Guangzhou Medical UniversityGuangzhou 510182China Department of Clinical Laboratory The Third Affiliated Hospital of Guangzhou Medical UniversityGuangzhou 510150China

Chlamydia trachomatis (CT) infection is the most prevalent sexually transmitted bacterial disease worldwide. However, unlikethat in female infertility, the role of CT infection in male infertility remains controversial. The objective of this retrospective studywas to explore the impacts of CT infection in the genital tract on sperm quality, sperm acrosin activity, antisperm antibody levels,and inflammation in a large cohort of infertile males in China. A total of 7154 semen samples were collected from infertile malesubjects, 416 of whom were CT positive (CT+ group) and 6738 of whom were CT negative (CT− group), in our hospital betweenJanuary 2016 and December 2018. Routine semen parameters (semen volume, pH, sperm concentration, viability, motility,morphology, etc.), granulocyte elastase levels, antisperm antibody levels, and sperm acrosin activity were compared between theCT+ and CT− groups. Our results showed that CT infection was significantly correlated with an abnormally low semen volume, aswell as an increased white blood cell count and granulocyte elastase level (all P < 0.05) in the semen of infertile males;otherroutine semen parameters were not negatively impacted. The antisperm antibody level and sperm acrosin activity were not affectedby CT infection. These findings suggested that CT infection might contribute to inflammation and hypospermia but does not impairsperm viability, motility morphology, and acrosin activity or generate antisperm antibodies in the infertile males of China.

关键词： Chlamydia trachomatis genital tract obstruction hypospermia male infertility semen parameters sperm quality

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Genome wide abnormal DNA methylome of human blastocyst in assisted reproductive technology

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Journal of Genetics and Genomics 2017年第10期44卷 475-481页

作者： Guoqiang Li Yang Yu Yong Fan Congru Li Xiaocui Xu Jialei Duan Rong Li Xiangjin Kang Xin Ma Xuepeng chen Yuwen Ke Jie Yan Ying Lian Ping Liu Yue Zhao Hongcui Zhao yaoyong chen Xiaofang Sun Jianqiao Liu Jie Qiao Jiang Liu Ministry of Education Key Laboratory of Assisted Reproduction Beijing Key Laboratory of Reproductive Endocrinology and Assisted ReproductiveTechnology Center of Reproductive Medicine Peking University Third Hospital Beijing 100191 China GAS Key Laboratory of Genome Sciences and Information Collaborative Innovation Center of Genetics and Development Beijing Institute of GenomicsGAS Beijing 160101 China Key Laboratory for Major Obstetric Diseases of Guangdong Province The Third Affiliated Hospital of Guangzhou Medical University Guangzhou 510150China University of Chinese Academy of Sciences Beijing 100029 China

Proper reprogramming of parental DNA methylomes is essential for mammalian embryonic development. However, it is unknown whether abnormal methylome reprogramming occurs and is associated with the failure of embryonic development. Here we analyzed the DNA methylomes of 57 blastocysts and 29 trophectoderm samples with different morphological grades during assisted reproductive technology （ART） practices. Our data reveal that the global methylation levels of high-quality blastocysts are similar （0.30 ± 0.02, mean ± SD）, while the methylation levels of low-quality blastocysts are divergent and away from those of high-quality blastocysts. The proportion of blastocysts with a methylation level falling within the range of 0.30± 0.02 in different grades correlates with the live birth rate for that grade. Moreover, abnormal methylated regions are associated with the failure of embryonic development. Furthermore, we can use the methylation data of cells biopsied from trophectoderm to predict the blastocyst methylation level as well as to detect the aneuploidy of the blastocysts. Our data indicate that global abnormal methylome reprogramming often occurs in human embryos, and suggest that DNA methylome is a potential biomarker in blastocyst selection in ART.

关键词： Human Abnormal Blastocyst Methylome ART

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Fargeted elimination of mutant mitochondria DNA in MELAS-iPSCs by mitoTALENs

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Protein & Cell 2018年第3期9卷 283-297页

作者： Yi Yang Han Wu Xiangjin Kang Yanhui Liang Ting Lan Tianjie Li Tao Tan Jiangyun Peng Quanjun Zhang Geng An Yali Liu Qian Yu Zhenglai Ma Ying Lian Boon Seng Soh Qingfeng chen Ping Liu yaoyong chen Xiaofang Sun Rong Li Xiumei Zhen Yang Yu Xiaoping Li Yong Fan Key Laboratory for Major Obstetric Diseases of Guangdong Province Key Laboratory of Reproduction and Genetics of Guangdong Higher Education Institutes The Third Affiliated Hospital of Guangzhou Medical University Guangzhou 510150 China Key Laboratory of Regenerative Biology of the Chinese Academy of Sciences and Guangdong Provincial Key Laboratory of Stem Cells and Regenerative Medicine South China Institute for Stem Cell Biology and Regenerative Medicine Guangzhou Institutes of Biomedicine and Health Chinese Academy of Sciences Guangzhou 510530 China Center of Reproductive Medicine Department of Obstetrics and Gynecology Peking University Third Hospital Beijing 100191 China Yunnan Key Laboratory of Primate Biomedical Research Institute of Primate Translational Medicine Kunming University of Science and Technology Kunming 650500 China Disease Modeling and Therapeutics Laboratory A'STAR Institute of Molecular and Cell Biology 61 Biopolis Drive Proteos Singapore 138673 Singapore Department of Biological Sciences National University of Singapore Singapore 117543 Singapore

Mitochondrial diseases are maternally inherited hetero- geneous disorders that are primarily caused by mitochondrial DNA （mtDNA） mutations. Depending on the ratio of mutant to wild-type mtDNA, known as heteroplasmy, mitochondrial defects can result in a wide spectrum of clinical manifestations. Mitochondria-targeted endonucleases provide an alternative avenue for treating mitochondrial disorders via targeted destruc- tion of the mutant mtDNA and induction of heteroplasmic shifting. Here, we generated mitochondrial disease patient-specific induced pluripotent stem cells （MiPSCs） that harbored a high proportion of m.3243A〉G mtDNA mutations and caused mitochondrial encephalomyopathy and stroke-like episodes （MELAS）. We engineered mitochondrial-targeted transcription activator-like effector nucleases （mitoTALENs） and successfully eliminated the m.3243A〉G mutation in MiPSCs. Off-target mutagenesis was not detected in the targeted MiPSC clones. Utilizing a dual fluorescence iPSC reporter cell line expressing a 3243G mutant mtDNA sequence in the nuclear genome, mitoTALENs displayed a significantly limited ability to target the nuclear genome compared with nuclear-localized TALENs. Moreover, genetically rescued MiPSCs displayed normal mitochondrial respiration and energy production. Moreover, neuronal progenitor cells differentiated from the rescued MiPSCs also demonstrated normal metabolic profiles. Further- more, we successfully achieved reduction in the human m.3243A〉G mtDNA mutation in porcine oocytes via injection of mitoTALEN mRNA. Our study shows the great potential for using mitoTALENs for specific targeting of mutant mtDNA both in iPSCs and mammalian oocytes, which not only provides a new avenue for studying mitochondrial biology and disease but also suggests a potential therapeutic approach for the treatment of mitochondrial disease, as well as the prevention of germline transmission of mutant mtDNA.

关键词： mitochondria iPSCs TALEN MELAS

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IGF2BP3-induced activation of EIF5B contributes to progression of hepatocellular carcinoma cells

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Oncology Research 2022年第2期30卷 77-87页

作者： XIAOYIN LI QIAN WANG HONGFENG LIANG SHISHENG chen HAIWEN chen yaoyong LU CHANGFU YANG Department of Oncology(Section 3) Gaozhou People’s HospitalGaozhouChina South China Research Center for Acupuncture and Moxibustion Medical College of Acu-Moxi and RehabilitationGuangzhou University of Chinese MedicineGuangzhouChina The Second Affiliated Hospital of Guangzhou University of Chinese Medicine(Guangdong Provincial Hospital of Chinese Medicine) GuangzhouChina Affiliated Nanfang Hospital(Branch Tai He Hospital)of Southern Medical University GuangzhouChina

In this study,we investigated the functional role of eukaryotic initiation factor 5B(EIF5B)in hepatocellular carcinoma(HCC)and the underlying mechanisms.Bioinformatics analysis demonstrated that the EIF5B transcript and protein levels as well as the EIF5Bcopy number were signiﬁcantly higher in the HCC tissues compared with the non-cancerous liver tissues.Down-regulation of EIF5B signiﬁcantly decreased proliferation and invasiveness of the HCC cells.Furthermore,EIF5B knockdown suppressed epithelial-mesenchymal transition(EMT)and the cancer stem cell(CSC)phenotype.Down-regulation of EIF5B also increased the sensitivity of HCC cells to 5-ﬂuorouracil(5-FU).In the HCC cells,activation of the NF-kappa B signaling pathway and IkB phosphorylation was signiﬁcantly reduced by EIF5B silencing.IGF2BP3 increased the stability of the EIF5B mRNA in an m6A-dependent manner.Our data suggested that EIF5B is a promising prognostic biomarker and therapeutic target in HCC.

关键词： EIF5B NF-jB Epithelial-mesenchymal transition Cancer stem cells N6-methyladenosine Hepatocellular carcinoma

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Microstructure and Property of AlN Joint Brazed with Au–Pd–Co–Ni–V Brazing Filler

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Journal of Materials Science & Technology 2015年第10期31卷 1034-1038页

作者： Bo chen Huaping Xiong yaoyong cheng Wei Mao Shibiao Wu Welding and Plastic Forming Division Beijing Institute of Aeronautical Materials

An Au-Pd-Co-Ni-V brazing alloy was designed for AIN ceramic joining. Its wettability on AIN was studied with the sessile drop method. The results showed that the contact angle was decreased gradu- ally with increasing temperature and the prolonging of holding time. Sound AIN/AIN joints were achieved with the brazing alloy at 1170 ℃ for 10 min. The microstructure of the AIN/AIN joints was examined by scanning electron microscopy （SEM）. It was found that element V played the active role in the interfacial reaction between the ceramic and the brazing alloy, V reacted with N decomposed from AIN, resulted in the formation of V-N compound. Based on the energy-dispersive spectroscopy （EDS） and X-ray diffraction （XRD） analysis results, the V-N reaction product was verified as V2N. The overall reaction during the brazing process can be described by the following equation： 2V ＋ AIN ＋ 2Pd = V2N ＋ Pd2AI. The AIN/AIN joints brazed with the Au-Pd-Co-Ni-V brazing alloy exhibited three-point bend strength of 162.7 MPa at room temperature, and under the bend test the fracture of the joint occurred at the AIN ceramic substrate.

关键词： AIN Brazing Interracial reaction Joints

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Joining of C_f/SiBCN composite with two Ni-based brazing fillers and interfacial reactions

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Journal of Materials Science & Technology 2017年第5期33卷 487-491页

作者： Wenwen Li Bo chen Yi Xiong Huaping Xiong yaoyong cheng Wenjiang Zou Welding and Plastic Forming Division Beijing Institute of Aeronautical Materials School of Materials University of Manchester

G/SiBCN ceramic composite was joined using Ni-19Cr-10Si （BNi5） and Ni-33Cr-24Pd-3.5Si-0.5B filler alloys at 1170 ℃ for 10 min. Two kinds of Ni-based filler alloys exhibited good wettability on the CdSiBCN com- posite, with a contact angle of 13° and 4°, respectively, The microstructures of the brazed joints were investigated by electron-probe microanalysis （EPMA）, and three-point bend test was conducted for the joints at room temperature. When being brazed with BNi5 filler alloy, no evident reaction layer was ob- served at the surface of the joined composite, and the joint microstructure was characterized by Ni2Si matrix with scatteringly distributing mixture compounds of Cr23C6, Ni2Si and CrB. While Ni-Cr-Pd（Si,B） brazing alloy was used, a Cr23C6 reaction layer with a thickness of 11 μm was formed at the surface of the base composite. In the central part of the brazed joint, the phases were composed of Ni（Cr, Si） solid solution and complex compounds including Pd2Si, （Ni,Pd）2Si and Ni-B. The strength of Cf/SiBCN joint brazed with BNi5 filler alloy was 62.9 MPa at room temperature, whereas that with Ni-Cr-Pd（Si,B） filler alloy was at the same level.

关键词： Cf/SiBCN composite Brazing Microstructure Joint strength Interracial reaction

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2205双相不锈钢双丝CMT焊接工艺与焊缝组织特征研究

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热加工工艺 2022年第1期51卷 147-151页

作者：刘邵房卫萍易耀勇邓运来余陈广东省焊接技术研究所(广东省中乌研究院)广东省现代焊接技术重点实验室广东广州510650 中南大学材料科学与工程学院湖南长沙410083

在5 mm厚度的2205双相不锈钢板上,采用药芯焊丝双丝CMT焊接技术进行堆焊试验,对不同工艺参数下的焊缝形貌进行分析。在堆焊的工艺基础上选取前丝脉冲电流271 A,后丝冷金属过渡复合脉冲电流200 A的焊接参数进行45°和60°坡口对接焊。结... 详细信息

在5 mm厚度的2205双相不锈钢板上,采用药芯焊丝双丝CMT焊接技术进行堆焊试验,对不同工艺参数下的焊缝形貌进行分析。在堆焊的工艺基础上选取前丝脉冲电流271 A,后丝冷金属过渡复合脉冲电流200 A的焊接参数进行45°和60°坡口对接焊。结果表明:堆焊焊缝的熔宽随着前丝电流的增大变化明显,而熔深和余高则变化不大,热影响区的硬度高于母材和焊缝区的。对接焊接头焊缝区可见三种不同形态的奥氏体相,其中奥氏体体含量在40%〜60%,满足焊接要求。从显微组织可看出60°坡口较45°坡口焊缝晶粒尺寸均匀细小。

关键词：双相不锈钢双丝CMT焊接焊缝成形显微组织

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Pd-Ni-(Cr，V)基高温钎料对Si_3N_4陶瓷的润湿及界面反应

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金属学报 2008年第10期44卷 1260-1264页

作者：陈波熊华平毛唯程耀永李晓红北京航空材料研究院焊接及锻压工艺研究室北京100095

采用座滴法研究了4种钎料Pd60-Ni40.PdNi-(3-6)V,PdNi-(7-15)V和PdNi-(16-24)Cr-(6-15)V(质量分数,%)在Si_3N_4陶瓷上的润湿性.结果表明,在1250℃/30 min的真空加热条件下,4种钎料均在Si_3N_4母材上润湿铺展,PdNi-(16—24)Cr-(6—15)V钎料不但润湿角小,而且形成了无缺陷的润湿界面.该钎料与Si_3N_4形成的扩散反应层中.Cr和V优先与从母材扩散出的N反应生成相应的Cr-N和V-N相;只有少量的Si参与反应生成相应的Cr—Si和V-Si相;钎料基体区中主要是溶有少量Si的Pd-Ni固溶体以及Pd-Si和Ni—Si相.Cr相对于V更容易向界面扩散.

关键词： Si3N4 反应层润湿性钎料

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基于VMAT放疗技术联合化疗加用尼妥珠单抗治疗EGFR表达阳性的Ⅳa期鼻咽癌的临床研究

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中国医药科学 2022年第24期12卷 148-151页

作者：陈海文卢耀勇张坤强广东省高州市人民医院广东高州525200

目的探究表皮生长因子受体(EGFR)表达阳性的Ⅳa期鼻咽癌患者采用容积调强放射治疗(VMAT)放疗技术联合化疗加用尼妥珠单抗治疗的临床效果。方法选择2021年4—11月高州市人民医院收治的80例鼻咽癌患者为研究对象,均为EGFR表达阳性的Ⅳa... 详细信息

目的探究表皮生长因子受体(EGFR)表达阳性的Ⅳa期鼻咽癌患者采用容积调强放射治疗(VMAT)放疗技术联合化疗加用尼妥珠单抗治疗的临床效果。方法选择2021年4—11月高州市人民医院收治的80例鼻咽癌患者为研究对象,均为EGFR表达阳性的Ⅳa期患者。根据治疗方案分为两组,对照组VMAT放疗+化疗,试验组VMAT放疗+化疗+尼妥珠单抗,每组各40例。比较两组鼻咽癌客观缓解率(ORR)、完全缓解率(CR)、无进展生存期(PFS)、肿瘤学指标、毒副反应。结果试验组ORR显著高于对照组,差异有统计学意义(P0.05)。试验组PFS较对照组长,差异有统计学意义(P0.05)。治疗后两组EBV DNA定量较治疗前低,且试验组低于对照组,差异有统计学意义(P0.05)。结论基于VMAT放疗技术联合化疗加用尼妥珠单抗治疗的方案用于EGFR表达阳性的Ⅳa期鼻咽癌患者临床治疗,疗效确切,值得推广使用。

关键词：鼻咽癌表皮生长因子受体阳性容积调强放射治疗尼妥珠单抗肿瘤学指标毒副反应

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药芯焊丝的稀土元素对921A钢焊缝金属组织与力学性能的影响

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热加工工艺 2017年第5期46卷 1-5页

作者：陈锐王凯易耀勇杜晓东合肥工业大学材料科学与工程学院安徽合肥230009 广东省焊接技术研究所(广东省中乌研究院)广东省现代焊接重点实验室广东广州510651

通过调整与控制600 MPa级高强钢药芯焊丝中的稀土元素含量,研制了适用于921A钢的气保护焊接用药芯焊丝,并利用制备出的药芯焊丝焊接921A钢。测定了焊缝金属的力学性能、显微硬度及低温冲击韧性,同时利用扫描电镜和金相显微镜对其拉伸试... 详细信息

通过调整与控制600 MPa级高强钢药芯焊丝中的稀土元素含量,研制了适用于921A钢的气保护焊接用药芯焊丝,并利用制备出的药芯焊丝焊接921A钢。测定了焊缝金属的力学性能、显微硬度及低温冲击韧性,同时利用扫描电镜和金相显微镜对其拉伸试样断口以及焊缝金属的组织形貌进行了观察。结果表明,在一定含量范围内,稀土元素的添加可以明显改善焊缝金属组织,提高低温冲击韧性和综合力学性能。稀土含量过高时,组织反而朝着不利于力学性能提升的方向转变。

关键词：高强钢药芯焊丝稀土力学性能显微组织

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