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A micropore array-based solid lift-off method for highly efficient and controllable cell alignment and spreading

Microsystems & Nanoengineering 2020年第1期6卷 517-529页

作者： Tingting Hun Yaoping Liu Yechang Guo Yan Sun Yubo Fan Wei Wang Key Laboratory for Biomechanics and Mechanobiology of Ministry of Education School of Biological Science and Medical EngineeringBeihang University100191 BeijingChina Institute of Microelectronics Peking University100871 BeijingChina State Key Laboratory of Transducer Technology Chinese Academy of Sciences200050 ShanghaiChina Beijing Advanced Innovation Center for Biomedical Engineering Beihang University100083 BeijingChina National Key Laboratory of Science and Technology on Micro/Nano Fabrication 100871 BeijingChina Frontiers Science Center for Nano-optoelectronics Peking University100871 BeijingChina

Interpretation of cell–cell and cell-microenvironment interactions is critical for both advancing knowledge of basic biology and promoting applications of regenerative medicine.Cell patterning has been widely investigated in previous studies.However,the reported methods cannot simultaneously realize precise control of cell alignment and adhesion/spreading with a high efficiency at a high throughput.Here,a novel solid lift-off method with a micropore array as a shadow mask was proposed.Efficient and precise control of cell alignment and adhesion/spreading are simultaneously achieved via an ingeniously designed shadow mask,which contains large micropores(capture pores)in central areas and small micropores(spreading pores)in surrounding areas contributing to capture/alignment and adhesion/spreading control,respectively.The solid lift-off functions as follows:(1)protein micropattern generates through both the capture and spreading pores,(2)cell capture/alignment control is realized through the capture pores,and(3)cell adhesion/spreading is controlled through previously generated protein micropatterns after lift-off of the shadow mask.High-throughput(2.4–3.2×10^(4) cells/cm 2)cell alignments were achieved with high efficiencies(86.2±3.2%,56.7±9.4%and 51.1±4.0%for single-cell,double-cell,and triple-cell alignments,respectively).Precise control of cell spreading and applications for regulating cell skeletons and cell–cell junctions were investigated and verified using murine skeletal muscle myoblasts.To the best of our knowledge,this is the first report to demonstrate highly efficient and controllable multicell alignment and adhesion/spreading simultaneously via a simple solid lift-off operation.This study successfully fills a gap in literatures and promotes the effective and reproducible application of cell patterning in the fields of both basic mechanism studies and applied medicine.

关键词： alignment pores solid

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A Compact Implementation of AES S-Box Using Evolutionary Algorithm

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Chinese Journal of Electronics 2017年第4期26卷 688-695页

作者： liu yaoping WU Ning ZHANG Xiaoqiang ZHOU Fang GE Fen College of Electronic and Information Engineering Nanjing University of Aeronautics and Astronautics

S-Box based on Composite field arithmetic（CFA） technology is optimized by Genetic algorithm（GA）and Cartesian genetic programming（CGP） model for reducing the hardware complexity. After using the CFA technique to map Multiplicative inverse（MI） over GF（2;）into composite field GF(（2;）;), the compact MI circuit over GF（2;） is selected from 100 evolved circuits, and same design method is applied to the compact multiplication circuit over GF（2;）. Compared with the direct implementations, the areas of optimized circuits of MI over GF（2;） and multiplication over GF(（2;）2) are reduced by66% and 57.69%, respectively. The area reductions for MI over GF（2;） and the whole of S-Box are up to 59.23%and 56.14%, separately. In 180 nm 1.8V COMS technology,compared to previous works, the S-Box proposed in this paper has the minimum area and minimum power, which are 11.27% and 6.65% smaller than that of the smallest area S-Box, respectively.

关键词： Advanced encryption standard(AES) Composite field arithmetic(CFA) S-Box Evolutionary al gorithm(EA)

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2.5-Dimensional Parylene C micropore array with a large area and a high porosity for high-throughput particle and cell separation

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Microsystems & Nanoengineering 2018年第1期4卷 286-297页

作者： Yaoping Liu Han Xu Wangzhi Dai Haichao Li Wei Wang Institute of Microelectronics Peking UniversityBeijing 100871China Department of Respirology No.1 Hospital of Peking UniversityBeijing 100034China National Key Laboratory of Science and Technology on Micro/Nano Fabrication Beijing 100871China Innovation Center for Micro-Nanoelectronics and Integrated Systems Beijing 100871China

Large-area micropore arrays with a high porosity are in high demand because of their promising potential in liquid biopsy with a large volume of clinical sample.However,a micropore array with a large area and a high porosity faces a serious mechanical strength challenge.The filtration membrane may undergo large deformation at a high filtration throughput,which will decrease its size separation accuracy.In this work,a keyhole-free Parylene molding process has been developed to prepare a large(>20 mm×20 mm)filtration membrane containing a 2.5-dimensional(2.5D)micropore array with an ultra-high porosity(up to 91.37% with designed pore diameter/space of 100μm/4μm).The notation 2.5D indicates that the large area and the relatively small thickness(approximately 10μm)of the fabricated membranes represent 2D properties,while the large thickness-to-width ratio(10μm/<4μm)of the spaces between the adjacent pores corresponds to a local 3D feature.The large area and high porosity of the micropore array achieved filtration with a throughput up to 180 mL/min(PBS solution)simply driven by gravity.Meanwhile,the high mechanical strength,benefiting from the 2.5D structure of the micropore array,ensured a negligible pore size variation during the high-throughput filtration,thereby enabling high size resolution separation,which was proven by single-layer and multi-layer filtrations for particle separation.Furthermore,as a preliminary demonstration,the prepared 2.5-dimensional Parylene C micropore array was implemented as an efficient filter for rare cancer cell separation from a large volume,approximately 10 cells in 10 mL PBS and undiluted urine,with high recovery rates of 87±13% and 56±13%,respectively.

关键词： Parylene separation porosity

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Enhanced YOLOv5 network-based object detection(BALFilter Reader)promotes PERFECT filter-enabled liquid biopsy of lung cancer from bronchoalveolar lavage fluid(BALF)

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Microsystems & Nanoengineering 2023年第5期9卷 177-189页

作者： Zheng liu Jixin Zhang Ningyu Wang Yun’ai Feng Fei Tang Tingyu Li Liping Lv Haichao Li Wei Wang Yaoping Liu School of Software and Microelectronics Peking UniversityBeijing 100871China Department of Pathology Peking University First HospitalBeijing 100034China School of Integrated Circuits Peking UniversityBeijing 100871China Department of Respirology and Critical Care Medicine Peking University First HospitalBeijing 100034China Department of Interventional Lung Disease and Center of Endoscopic Diagnosis and Treatment Anhui Chest HospitalHefeiAnhui 230022China National Key Laboratory of Advanced Micro and Nano Manufacture Technology Beijing 100871China Frontiers Science Center for Nano-optoelectronics Peking UniversityBeijing 100871China AntiMicrobial Resistance(AMR)and Critical Analytics for Manufacturing Personalized-Medicine(CAMP)IRGs Singapore-MIT Alliance for Research and Technology(SMART)CenterSingapore 138602Singapore

Liquid biopsy of cancers,detecting tumor-related information from liquid samples,has attracted wide attentions as an emerging technology.Our previously reported large-area PERFECT(Precise-Efficient-Robust-Flexible-Easy-ControllableThin)filter has demonstrated competitive sensitivity in recovering rare tumor cells from clinical samples.However,it is time-consuming and easily biased to manually inspect rare target cells among numerous background cells distributed in a large area(Φ≥13 mm).This puts forward an urgent demand for rapid and bias-free inspection.Hereby,this paper implemented deep learning-based object detection for the inspection of rare tumor cells from large-field images of PERFECT filters with hematoxylin-eosin(HE)-stained cells recovered from bronchoalveolar lavage fluid(BALF).CenterNet,EfficientDet,and YOLOv5 were trained and validated with 240 and 60 image blocks containing tumor and/or background cells,respectively.YOLOv5 was selected as the basic network given the highest mAP@0.5 of 92.1%,compared to those of CenterNet and EfficientDet at 85.2%and 91.6%,respectively.Then,tricks including CIoU loss,image flip,mosaic,HSV augmentation and TTA were applied to enhance the performance of the YOLOv5 network,improving mAP@0.5 to 96.2%.This enhanced YOLOv5 network-based object detection,named as BALFilter Reader,was tested and cross-validated on 24 clinical cases.The overall diagnosis performance(~2 min)with sensitivity@66.7%±16.7%,specificity@100.0%±0.0%and accuracy@75.0%±12.5%was superior to that from two experienced pathologists(10–30 min)with sensitivity@61.1%,specificity@16.7%and accuracy@50.0%,with the histopathological result as the gold standard.The AUC of the BALFilter Reader is 0.84±0.08.Moreover,a customized Web was developed for a user-friendly interface and the promotion of wide applications.The current results revealed that the developed BALFilter Reader is a rapid,bias-free and easily accessible AI-enabled tool to promote the transplantation of the BALFilter technique.This work can easily

关键词： specificity network consuming

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Modeling the radiation balance of different urban underlying surfaces

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Chinese Science Bulletin 2012年第9期57卷 1046-1054页

作者： CUI yaoping liu JiYuan HU YunFeng WANG JunBang KUANG WenHui Institute of Geographic Sciences and Natural Resources Research Chinese Academy of Sciences Beijing 100101 China Graduate University of Chinese Academy of Sciences Beijing 100049 China

An urban net all-wave radiation parameterization scheme is evaluated using annual datasets for 2010 recorded at a Beijing urban observation site.The statistical relationship between observed data and simulation data of net radiation has a correlation coefficient of 0.98 and model efficiency of 0.93.Therefore,it can be used to simulate the radiation balance of Beijing.This study analyzes the variation in the radiation balance for different underlying surfaces.To simulate radiation balance differences,we set four pure land-cover types(forest,grass,roads,and buildings).Keeping all other conditions inputted unchanged,we model the radiation balance by changing the land-cover type.The results show that the effects of different underlying surfaces on radiation differ,and that there is much upward long-wave radiation,accounting for 84.3% of the total radiation energy falling incident on the land surface.The annual averages of net radiation for the four land-cover types are in the range of 38.2-53.4 W/m2.The net radiation of the grass surface is minimal while that of the roads surface is maximal.Additionally,with urbanization the net radiation values of common types of land-cover change,such as conversion from forest to roads,grass to roads,and grass to buildings,all have increasing trends,indicating that net radiation usually increases with urban sprawl.

关键词：辐射平衡城市化下垫面土地覆盖类型建模模拟数据平衡模型参数化方案

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35 km amplifier-less four-level pulse amplitude modulation signals enabled by a 23 GHz ultrabroadband directly modulated laser

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Journal of Semiconductors 2020年第3期41卷 45-48页

作者： yaoping Xiao Yu liu Yiming Zhang Haotian Bao Ninghua Zhu State Key Laboratory on Integrated Optoelectronics Institution of SemiconductorsChinese Academy of SciencesBeijing 100083China School of Electronic Electrical and Communication EngineeringUniversity of Chinese Academy of SciencesBeijing 100049China

The 4-level pulse amplitude modulation(PAM4)based on an 23 GHz ultrabroadband directly modulated laser(DML)was proposed.We have experimentally demonstrated that based on intensity modulation and direct detection(IMDD)56 Gbps per wavelength PAM4 signals transferred over 35 km standard single mode fiber(SSMF)without any optical amplification and we have achieved the bit error rate(BER)of the PAM4 transmission was under 2.9×10–4 by using feed forward equalization(FFE).

关键词： directly modulated laser PAM4 FFE IMDD

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基于移位内插互质阵列高分辨DOA估计

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传感器与微系统 2022年第1期41卷 24-27页

作者：曾耀平刘洋西安邮电大学通信与信息工程学院陕西西安710121

针对互质阵列现有算法中可估计信源数少,自由度仍低的问题。提出一种新的虚拟域波达方向(DOA)估计算法。首先,对一般互质阵列改进形成移位互质阵列,通过差集数组形成虚拟阵列,增加了阵元个数,提高了阵列自由度;其次,通过虚拟阵元内插对... 详细信息

针对互质阵列现有算法中可估计信源数少,自由度仍低的问题。提出一种新的虚拟域波达方向(DOA)估计算法。首先,对一般互质阵列改进形成移位互质阵列,通过差集数组形成虚拟阵列,增加了阵元个数,提高了阵列自由度;其次,通过虚拟阵元内插对虚拟域中缺失孔洞进行填充,形成连续虚拟阵列;最后,通过最小化原子范数的设计和内插虚拟阵列协方差矩阵的重建,保证DOA估计的准确性。改进算法在提升阵列自由度的基础上,将虚拟域全部信息用于DOA估计,并且避免了预定义网格点带来的基不匹配问题,计算机仿真实验验证了算法的有效性。

关键词：移位互质阵列虚拟内插无网格化稀疏重建

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刘要萍作品

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经济师 2023年第6期 F0003-F0004页

作者：刘要萍太原市美术家协会太原市光线书画院太原市企业家联合会书画院万柏林区美协

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Some possible station layouts and characteristics of evacuated tube transportation

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Journal of Modern Transportation 2011年第3期19卷 181-185页

作者： yaoping ZHANG Benlin liu Yong ZHAO Institute of Vacuum Tube Transportation Xijing University Xi ＇an 710123 China Superconductivity and New Energy Center （SNEC） Southwest Jiaotong University Chengdu 610031 China

Since Maglev vehicles will run in a closed vacuum tube,the layout of the terminal stations of evacuated tube transportation（ETT） will differ from the traditional railway stations.This paper deals with some possible station layouts of ETT,e.g.,a station with an airlock,a station without an airlock,above ground and underground stations,and stations with either level arrayed or rotation platforms.Then different station layouts are compared,and characteristics of each are analyzed.Finally,a more secure mode for ETT station layouts is suggested,which can be the basis for future ETT station layout and designs.

关键词： station layout terminal vacuum evacuated tube transportation airlock

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毛泽东全面抗战思想的生成逻辑及其内涵特征

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毛泽东研究 2021年第1期 75-83页

作者：刘大禹胡耀平江南大学马克思主义学院湖南科技大学马克思主义学院

抗日战争期间,毛泽东作为中国共产党的主要领导人,通过理论思考与实践积累,形成了系统的全面抗战思想。这一思想的形成逻辑严密、内涵丰富、特征明显,是中华民族坚持抗战到底的精神武器与科学指南,有效指导了全民族团结抗战与全面抗战。

关键词：毛泽东全面抗战生成逻辑内涵特征

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