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Single-nucleus transcriptomics reveals a gatekeeper role for FoxP1 in primate cardiac aging

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Protein & Cell 2023年第4期14卷 279-293页

作者： Yiyuan Zhang Yandong Zheng Si Wang Yanling Fan Yanxia Ye Yaobin Jing Zunpeng Liu Shanshan Yang Muzhao Xiong Kuan Yang jinghao Hu Shanshan Che Qun Chu Moshi Song Guang-Hui Liu Weiqi Zhang Shuai Ma jing Qu National Laboratory of Biomacromolecules CAS Center for Excellence in BiomacromoleculesInstitute of BiophysicsChinese Academy of SciencesBeijing 10o101China State Key Laboratory of Stem Cell and Reproductive Biology Institute of ZoologyChinese Academy of SciencesBeijing 100101China State Key Laboratory of Membrane Biology Institute of ZoologyChinese Academy of SciencesBeijing 100101China University of Chinese Academy of Sciences Beijing100049China Institute for Stem Cell and Regeneration Chinese Academy of SciencesBeijing 100101China Beijing Institute for Stem Cell and Regenerative Medicine Beijing 100101China CAS Key Laboratory of Genomic and Precision Medicine Beijing Institute of GenomicsChinese Academy of SciencesBeijing 100101China China National Center for Bioinformation Beijing 100101China Aging Translational Medicine Center Xuanwu HospitalCapital Medical UniversityBeijing 100053China Advanced Innovation Center for Human Brain Protection National Clinical Research Center for Geriatric DisordersXuanwu Hospital Capital Medical UniversityBeijing 100053China The Fifth People's Hospital of Chongqing Chongqing 400062China Sino-Danish College University of Chinese Academy of SciencesBeijing 101408China

Aging poses a major risk factor for cardiovascular diseases,the leading cause of death in the aged population.However,the cell type-specific changes underlying cardiac aging are far from being clear.Here,we performed single-nucleus RNA-sequencing analysis of left ventricles from young and aged cynomolgus monkeys to define cell composition changes and transcriptomic alterations across different cell types associated with age.We found that aged cardiomyocytes underwent a dramatic loss in cell numbers and profound fluctuations in transcriptional profles.Via transcription regulatory network analysis,we identified FOxP1,a core transcription factor in organ development,as a key downregulated factor in aged cardiomyocytes,concomitant with the dysregulation of FoxP1 target genes associated with heart function and cardiac diseases.Consistently,the deficiency of FOxP1 led to hypertrophic and senescent phenotypes in human embryonic stem cell-derived cardiomyocytes.Altogether,our findings depict the celiular and molecular landscape of ventricular aging at the single-cell resolution,and identify drivers for primate cardiac aging and potential targets for intervention against cardiac aging and associated diseases.

关键词： single-nucleus RNA-sequencing primate aging FOxP1 cardiomyocyte

A novel, minimally invasive rat model of normothermic cardiopulmonary bypass model without blood priming

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Chinese Medical Journal 2014年第8期127卷 1541-1544页

作者： Zhu yaobin Liu Donghai Li Xiaofeng Liu Aijun Wang Qiang Qiao Chenhui Zhang jing Liao Qiuming ZhangYanbo Department of Pediatric Heart Center Beijing Anzhen Hospital Capital Medical University Beijing 100029 China Department of Pediatric Heart Center btate Ney caooratory m Cardiovascular Disease Fuwai Hospital National Center for Cardiovascular Disease Chinese Academy of Medical Sciences and Peking Union Medical College Beijing 100037 China( Department of Cardiothoracic Surgery Lund University Lund 22594Sweden

Background Cardiopulmonary bypass （CPB） has been shown to be associated with systemic inflammatory response leading to postoperative organ dysfunction. Elucidating the underlying mechanisms and developing protective strategies for the pathophysiological consequences of CPB have been hampered due to the absence of a satisfactory recovery animal model. The purpose of this study was to establish a novel, minimally invasive rat model of normothermic CPB model without blood priming. Methods Twenty adult male Sprague-Dawley rats weighing 450-560 g were randomly divided into CPB group （n=10） and control group （n=10）. All rats were anaesthetized and mechanically ventilated. The carotid artery and jugular vein were cannulated. The blood was drained from the right atrium via the right jugular and further transferred by a miniaturized roller pump to a hollow fiber oxygenator and back to the rat via the left carotid artery. The volume of the priming solution, composed of 6% HES130/0.4 and 125 IU heparin, was less than 12 ml. The surface of the hollow fiber oxygenator was 0.075 m2. CPB was conducted for 60 minutes at a flow rat of 100-120 ml. kg -1. min-1 in CPB group. Oxygen flow/perfusion flow was 0.8 to 1.0, and the mean arterial pressure remained 60-80 mmHg. Results All CPB processes were successfully achieved. Blood gas analysis and hemodynamic parameters of each time point were in accordance with normal ranges. The vital signs of all rats were stable. Conclusions The establishment of CPB without blood priming in rats can be achieved successfully. The nontransthoracic model should facilitate the investigation of pathophysiological processes concerning CPB-related multiple organ dysfunction and possible protective interventions. This novel, recovery, and reproducible minimally invasive CPB model may open the tield tor various studies on the pathophysiological process of CPB and systemic ischemia-reperfusion injury in vivo.

关键词： rats animal model cardiopulmonary bypass model lung injury

Enhanced heterogeneous Fenton-like activity by Cu-dopecl BiFeO3 perovskite for degradation of organic pollutants

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Frontiers of Environmental Science & Engineering 2018年第6期12卷 103-112页

作者： Jie Mao Xie Quan jing Wang Cong Gao Shuo Chen Hongtao Yu yaobin Zhang Key Laboratory of Industrial Ecology and Environmental Engineering (Ministry of Education) School of Environmental Science and TechnologyDalian University of TechnologyDalian 116024China

基于博弈论组合赋权的水工隧洞TBM施工地质适宜性评价模型

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安全与环境工程 2021年第2期28卷 95-101,108页

作者：景耀斌顾伟红翟强兰州交通大学土木工程学院甘肃兰州730070

为解决水工隧洞TBM施工中的地质适宜性问题,参考国内外水工隧洞TBM施工的相关实践经验,结合《工程岩体分级标准》(GB/T 50218-2014)中对围岩分类的方法,并考虑到TBM设备的运行状态,选取岩石单轴抗压强度等10个因素作为评价指标,建立水... 详细信息

为解决水工隧洞TBM施工中的地质适宜性问题,参考国内外水工隧洞TBM施工的相关实践经验,结合《工程岩体分级标准》(GB/T 50218-2014)中对围岩分类的方法,并考虑到TBM设备的运行状态,选取岩石单轴抗压强度等10个因素作为评价指标,建立水工隧洞TBM施工地质适宜性评价指标体系;评价指标的主观权重选择层次分析法确定,评价指标的客观权重选择熵权法确定,通过博弈论组合赋权法确定评价指标的综合权重;采用模糊综合评价方法,建立基于博弈论组合赋权的水工隧洞TBM施工地质适宜性多因素评价模型,并选择岭形函数确定各评价指标的模糊关系,研究各评价指标在不同的取值范围内水工隧洞TBM掘进的地质适宜性情况;将模型应用于新疆某个供水工程中,并将模型评价结果与施工现场实测数据进行了对比,结果表明:建立的水工隧洞TBM施工地质适宜性评价模型在实际工程中的应用效果较好,得到的评价结果与施工现场的实测数据相符合,从而验证了该模型的实用性和可靠性。

关键词：水工隧洞 TBM 地质适宜性评价模型博弈论组合赋权法

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基于神经网络与小波变换的滚动轴承故障诊断

机械设计与研究 2013年第6期29卷 33-35页

作者：胡耀斌谢静胡良斌南华大学机械工程学院湖南衡阳421001

神经网络是一种具有非线性映射能力强以及自学习、自组织、自适应等优点的智能方法,非常适合于滚动轴承的故障诊断。针对滚动轴承是机械设备重要的易损零件之一,大约有30%的故障是由轴承损坏引起的,提出了基于神经网络的滚动轴承故障诊... 详细信息

神经网络是一种具有非线性映射能力强以及自学习、自组织、自适应等优点的智能方法,非常适合于滚动轴承的故障诊断。针对滚动轴承是机械设备重要的易损零件之一,大约有30%的故障是由轴承损坏引起的,提出了基于神经网络的滚动轴承故障诊断方法。以滚动轴承小波分解后的能量信息作为特征,通过神经网络作为分类器对滚动轴承故障进行识别、诊断。实验表明,该方法对于滚动轴承的故障诊断具有良好的效果和应用价值,并可方便地推广到其他类似的诊断领域。

关键词：神经网络小波变换滚动轴承故障诊断

Characterisation of acute toxicity, genotoxicity and oxidative stress posed by textile effluent on zebrafish

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Journal of Environmental Sciences 2012年第11期24卷 1963-1971页

作者： Wenjuan Zhang, Wei Liu, jing Zhang, Huimin Zhao, yaobin Zhang, Xie Quan, Yihe Jin Key Laboratory of Industrial Ecology and Environmental Engineering, MOE, School of Environmental Science and Technology, Dalian University of Technology, Dalian 116024, China Key Laboratory of Industrial Ecology and Environmental Engineering MOE School of Environmental Science and Technology Dalian University of Technology Dalian 116024 China

Textile industries are important sources of toxic discharges and contribute enormously to water deterioration, while little attention has been paid to the toxicity of textile effluents in discharge regulation. Bioassays with zebrafish were employed to evaluate the toxicity of wastewater samples collected from different stages at a textile factory and sewage treatment plants (STPs). Physico-chemical parameters, acute toxicity, genotoxicity and oxidative stress biomarkers were analyzed. The wastewater samples from bleaching, rinsing and soaping of the textile factory exhibited high acute toxicity and genotoxicity. The coexisting components of dye compounds, as assistants and oxidants, seemed to cause some effect on the toxic response. After treatment employing the anoxic-oxic (A/O) process in STPs, the color and the chemical oxygen demand (COD) were reduced by 40% and 84%, respectively, falling within the criteria of the Chinese Sewage Discharge Standard. In contrast, increases in acute toxicity and genotoxicity were observed in the anaerobic tank, indicating the formation of toxic intermediates. The genotoxicity of the effluent of the STP was not significantly different from that of the influent, suggesting the wastewater treatment processes were not effective in removing the genotoxicity of the dye wastewater. Results indicated that the effluent contains pro-oxidants since the activities of glutathione (GSH), malondialdehyde (MDA), and total anti-oxidation capacity (T-AOC) were all elevated. In addition, decreases in superoxide dismutase (SOD) and glutathione-S transferase (GST) activities observed can be interpreted as a cytotoxicity sign due to an over-production of reactive oxygen species (ROS). The results of the present study suggest that the STPs were not capable of reducing the toxicity of wastewater sufficiently. Further treatment is needed to remove the potential risks posed by textile effluent to ecosystems and human health, and employing a toxicity index is necessary for discharge regulation.

关键词： acute toxicity genotoxicity micronucleus comet textile effluent

极近垂距骑跨采工作面边界位置优化及底板巷道围岩稳定性研究

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煤矿安全 2018年第7期49卷 28-33页

作者：郭靖宋要斌赵利鼎马利云吕梁学院矿业工程系山西吕梁033000

山西某矿骑跨采工作面与底板630轨道运输大巷垂距最小为6 m,为了提高煤炭采出率、保证底板大巷的长期稳定,应用弹塑性理论和数值模拟软件,建立工作面超前和侧向支承压力作用下的平面力学模型和数值模型,得到了底板大巷在超前高应力区和... 详细信息

山西某矿骑跨采工作面与底板630轨道运输大巷垂距最小为6 m,为了提高煤炭采出率、保证底板大巷的长期稳定,应用弹塑性理论和数值模拟软件,建立工作面超前和侧向支承压力作用下的平面力学模型和数值模型,得到了底板大巷在超前高应力区和采后低应力区的应力分布特征和变形规律。研究结果表明:跨采工作面开采边界位置距离底板巷道最佳水平距离为15 m。根据动压影响下底板大巷应力分布特征和变形规律,针对性地提出了"局部注浆+高强锚杆索"联合支护技术。现场监测结果表明:加固效果明显,保证了底板大巷的正常使用。

关键词：骑跨采工作面数值模拟超前支承压力侧向支承压力弹塑性

两种工程堆积体坡面细沟形态与产沙关系对比研究

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土壤学报 2021年第1期58卷 115-127页

作者：张恒高照良牛耀彬李永红赵晶陈卓苏媛西北农林科技大学水土保持研究所陕西杨凌712100 中国科学院水利部水土保持研究所陕西杨凌712100 生态环境部环境工程评估中心北京100012

为探究不同土壤类型工程堆积体坡面细沟形态与水动力学参数及侵蚀产沙间的关系,选取沟宽、沟深、宽深比等作为细沟形态参数,在放水流量8、12、16、20 L·min^(-1)和坡度32°条件下,对红土和塿土工程堆积体进行冲刷试验。结果表明:(1)两... 详细信息

为探究不同土壤类型工程堆积体坡面细沟形态与水动力学参数及侵蚀产沙间的关系,选取沟宽、沟深、宽深比等作为细沟形态参数,在放水流量8、12、16、20 L·min^(-1)和坡度32°条件下,对红土和塿土工程堆积体进行冲刷试验。结果表明:(1)两种堆积体坡面细沟沟宽、沟深在前24 min均发育迅速;随放水流量增大,沟宽和沟深均呈增大趋势,红土沟宽均值(较8 L·min^(–1))分别增加0.8%、19.3%、27.2%,沟深均值分别增加24.5%,126.2%,95.1%,而塿土则为3.7%、62.4%、108.2%和18.2%、87.9%、95.5%;红土沟宽、沟深与冲刷历时均呈极显著线性函数关系(R2>0.858,P0.689,P0.602,P0.897,P<0.01);沟宽、沟深对红土坡面累计产沙量的贡献率分别为84.9%、15.1%,对塿土则为22.5%和77.5%;该研究可为不同土壤类型工程堆积体坡面水土保持措施合理配置提供理论依据。

关键词：放水冲刷工程堆积体细沟形态产沙

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大行程直线运动平台自抗扰控制

机床与液压 2019年第14期47卷 7-10页

作者：何耀滨杨志军张炫山何海超贾静广东工业大学广东省微纳加工技术与装备重点实验室广东广州510006 三英精控(天津)科技有限公司天津301700

大行程直线运动平台是复杂的非线性时变系统,系统的不确定因素较多。针对平台运动过程中受到的内扰和外扰问题,采用线性自抗扰控制进行控制。建立系统的动力学模型,分析系统非线性的原因;将系统的速度、位移作为状态变量,设计一个线性... 详细信息

大行程直线运动平台是复杂的非线性时变系统,系统的不确定因素较多。针对平台运动过程中受到的内扰和外扰问题,采用线性自抗扰控制进行控制。建立系统的动力学模型,分析系统非线性的原因;将系统的速度、位移作为状态变量,设计一个线性自抗扰控制器。将异于积分标准性系统的部分视为系统的总扰动,并在控制器中进行补偿。在仿真与工程实验中,分别加载S形曲线、正弦曲线运动,并进行抗扰性实验,将实验结果与PID控制算法进行比较。实验结果表明:在外部条件相同的情况下,线性自抗扰控制器不仅响应速度快、精度高,并且具有较强的鲁棒性,具备较好的工程应用前景。

关键词：大行程直线平台线性自抗扰控制器总扰动不确定性