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Specific molecular markers in hepatocellular carcinoma

Hepatobiliary & Pancreatic Diseases International 2007年第3期6卷 241-247页

作者： yao, deng-fu Dong, Zhi-Zhen yao, Min Nantong Univ Affiliated Hosp Res Ctr Clin Mol Biol Nantong 226001 Peoples R China Nantong Univ Affiliated Hosp Dept Diagnost Nantong 226001 Peoples R China Nantong Univ Affiliated Hosp Dept Lab Med Nantong 226001 Peoples R China

BACKGROUND: The carcinogenesis of hepatocellular carcinoma (HCC) is a multi-factorial, multistep and complex process. Its prognosis is poor, and early diagnosis and monitoring metastasis of HCC is of the utmost importance. Circulating diagnostic and prognostic biomarkers could be used in proper postoperative treatment of patients at an early stage of HCC development. This review summarizes recent studies of the specific biomarkers in diagnosis and monitoring metastasis or postoperative recurrence of HCC. DATA SOURCES: An English-language literature search was conducted using MEDLINE (June 1998 to Spetember 2006) on researches of some valuable specific biomarkers in diagnosis and monitoring metastasis or postoperative recurrence of HCC. RESULTS: Hepatoma tissues can synthesize various tumor-related proteins, polypeptides, and isoenzymes, such as alpha-fetoprotein (AFP), hepatoma-specific gamma-glutamyl transpeptidase (HS-GGT), etc, and then secrete into blood. The valuable early diagnostic and prognostic biomarkers could predict the development an metastases of HCC. Recent researches have confirmed that circulating hepatoma-specific AFP subfraction, transforming growth factor (TGF)-beta 1, HS-GGT, and free insulin-like growth factor (IGF)-II may be more specific markers than total AFP level for early diagnosis for HCC. The circulating genetic markers such as AFP-mRNA, TGF-beta 1-mRNA, IGF-II-mRNA, etc from peripheral blood mononuclear cells of HCC patients have been most extensively used in monitoring distal metastasis or postoperative recurrence of HCC. CONCLUSIONS: Hepatoma tissues synthesize and secrete valuable molecular markers into blood. The analyses of circulating hepatoma-specific biomarkers are useful to early diagnosis of HCC or monitoring metastasis or postoperative recurrence of HCC.

关键词： carcinoma hepatocellular molecular marker early diagnosis metastasis of tumor postoperative recurrence

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Hepatobiliary & Pancreatic Diseases International 2007年第1期6卷 9-11页

作者： yao, deng-fu Dong, Zhi-Zhen Hosp Nantong Univ Affiliated Hosp Res Ctr Clin Mol Biol Nantong 226001 Peoples R China

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Delayed hepatocarcinogenesis through antiangiogenic intervention in the nuclear factor-kappa B activation pathway in rats

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Hepatobiliary & Pancreatic Diseases International 2010年第2期9卷 169-174页

作者： Dong, Zhi-Zhen yao, deng-fu Wu, Wei yao, Min Yu, Hong-Bo Shen, Jun-Jun Qiu, Li-Wei yao, Ning-Hua Sai, Wen-Li Yang, Jun-Ling Nantong Univ Affiliated Hosp Res Ctr Clin Med Nantong 226001 Peoples R China Nantong Univ Affiliated Hosp Dept Diagnost Coll Med Nantong 226001 Peoples R China Nantong Univ Affiliated Hosp Dept Lab Med Nantong 226001 Peoples R China Nantong Univ Affiliated Hosp Dept Oncol Nantong 226001 Peoples R China Nantong Canc Hosp Dept Chemotherapy Nantong 226007 Peoples R China

BACKGROUND: The active form of nuclear factor-kappa B (NF-kappa B) is involved in the initiation, generation, and development of hepatocellular carcinoma (HCC), and is up-regulated in inflammation-associated malignancies. We investigated the dynamic expression of NF-kappa B and its influences on the occurrence of HCC through antiangiogenic (thalidomide) intervention in NF-kappa B activation. METHODS : Hepatoma models were induced with 2-fluorenylacetamide (2-FAA, 0.05%) in male Sprague-Dawley rats, and thalidomide (100 mg/kg body weight) was administered intragastrically to intervene in NF-kappa B activation. The pathological changes in the liver of sacrificed rats were assessed after hematoxylin and eosin staining. NF-kappa B mRNA was amplified by RT-nested PCR. The alterations of NF-kappa B and vascular endothelial growth factor (VEGF) expression were analyzed by enzyme-linked immunosorbent assay, immunohistochemistry, and Western blotting. RESULTS: Rat hepatocytes showed denatured, precancerous, and cancerous stages in hepatocarcinogenesis, with an increasing tendency of hepatic NF-kappa B, NF-kappa B mRNA, and VEGF expression, and their values in the HCC group were higher than those in controls (P<0.001). In the thalidomide-treated group, the morphologic changes generated only punctiform denaturation and necrosis at the early or middle stages, and nodular hyperplasia or a little atypical hyperplasia at the final stages, with the expression of NF-kappa B (chi(2)=9.93, P<0.001) and VEGF (chi(2)=8.024, P<0.001) lower than that in the 2-FAA group. CONCLUSION: NF-kappa B is overexpressed in hepatocarcinogenesis and antiangiogenic treatment down-regulates the expression of NF-kappa B and VEGF, and delays the occurrence of HCC. (Hepatobiliary Pancreat Dis Int 2010; 9: 169-174)

关键词： hepatocellular carcinoma nuclear factor-kappa B vascular endothelial growth factor intervention dynamic expression

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Early monitoring values of oncogenic signalling molecules for hepatocellular carcinoma

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World Journal of Gastrointestinal Oncology 2024年第6期16卷 2350-2361页

作者： Min yao Rong-Fei Fang Qun Xie Min Xu Wen-Li Sai deng-fu yao Department of Immunology Medical School of Nantong University and Research Center of Clinical MedicineThe Affiliated Hospital of Nantong UniversityNantong 226001Jiangsu ProvinceChina Department of Gastroenterology The Affiliated Hospital of Nantong UniversityNantong 226001Jiangsu ProvinceChina Department of Infectious Diseases Haian People’s HospitalHaian 226600Jiangsu ProvinceChina Research Center of Clinical Medicine The Affiliated Hospital of Nantong UniversityNantong 226001Jiangsu ProvinceChina

The prevention and early diagnosis of liver cancer remains a global medical *** the malignant transformation of hepatocytes,a variety of oncogenic cellular signalling molecules,such as novel high mobility group-Box 3,angiopoietin-2,Golgi protein 73,glypican-3,Wnt3a(a signalling molecule in the Wnt/β-catenin pathway),and secretory clusterin,can be expressed and secreted into the *** signalling molecules are derived from different signalling pathways and may not only participate in the malignant transformation of hepatocytes but also become early diagnostic indicators of hepatocarcinogenesis or specific targeted molecules for hepatocellular carcinoma *** article reviews recent progress in the study of several signalling molecules as sensitive biomarkers for monitoring hepatocarcinogenesis.

关键词： Hepatocarcinogenesis Cell signals Specific biomarkers Early diagnosis

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细胞因子在肝细胞癌变中的作用与机制

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中华肝脏病杂志 2005年第5期13卷 378-379页

作者：姚登福南通大学附属医院 226001

肝脏是富有先天免疫反应细胞的重要器官,细胞因子在信号传导、转录调控、新血管形成等方面与肝细胞癌变关系极为密切.

关键词：细胞因子肝细胞癌肿瘤免疫反应

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Dynamic expression of rat heat shock protein gp96 and its gene during development of hepatocellular carcinoma

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Hepatobiliary & Pancreatic Diseases International 2007年第6期6卷 616-621页

作者： Wu, Xin-Hua yao, deng-fu Su, Xiao-Qin Tai, Bo-Jun Huang, Hua Qiu, Li-Wei Wu, Wei Shao, Yi-Xiang Nantong Univ Affiliated Hosp Res Ctr Clin Mol Biol Nantong 226001 Peoples R China Nantong Univ Affiliated Hosp Dept Infect Dis Nantong Peoples R China Nantong Univ Affiliated Hosp Dept Pathol Nantong Peoples R China Nantong Univ Ctr Med Lab Anim Nantong 226001 Peoples R China

BACKGROUND: Hepatocellular carcinoma (HCC) is characterized by multicause, obvious multistage and multifocal processes of tumor progression. The development of HCC is related intimately to overexpression and signal transduction of many cellular factors. This study was undertaken to investigate the dynamic expression and alteration of heat shock protein (HSP) gp96 along with its gene during HCC development. METHODS: A rat model of hepatoma induced with 2-fluorenylacetamide (2-FAA, 0.05%) was established in male Sprague-Dawley rats. Total RNA and pathological changes were observed during hepatocarcinogenesis. Total RNAs were transcribed into cDNA by reverse transcription and the gene fragment of gp96 was amplified by nested RT-PCR. The gp96 expression in rat liver tissues was semi-quantitatively analyzed by immunohistochemistry. RESULTS: Histological examination suggested that hepatocytes in rats fed with 2-FAA showed vacuole-like denaturation at the early stages, then dysplastic nodules appeared at the middle stage, and finally progressed to tubercles of cancerous nests. A tendency of increasing liver gp96 protein level was found from normal liver to precancerous to cancerous tissues during hepatoma development (Pful marker for early diagnosis.

关键词： hepatocellular carcinoma HSP gp96 RT-PCR immunohistochemistry

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Expression and alterations of different molecular form γ-glutamyl transferase and total RNA concentration during the carcinogenesis of rat hepatoma

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World Journal of Gastroenterology 1999年第4期5卷 84-86页

作者： TANG Qi Yun, yao deng fu, LU Jian Xin, WU Xin Hua and MENG Xian Yong

ＩＮＴＲＯＤＵＣＴＩＯＮＣａｒｃｉｎｏｇｅｎｅｓｉｓｉｓｃｌｏｓｅｌｙｒｅｌａｔｅｄｗｉｔｈＤＮＡｓｙｎｔｈｅｓｉｓａｎｄｈｙｐｅｒｍｅｔａｂｏｌｉｓｍｏｆｎｕｃｌｅｉｃａｃｉｄ．γｇｌｕｔａｍｙｌｔｒａｎｓｆｅｒ... 详细信息

ＩＮＴＲＯＤＵＣＴＩＯＮＣａｒｃｉｎｏｇｅｎｅｓｉｓｉｓｃｌｏｓｅｌｙｒｅｌａｔｅｄｗｉｔｈＤＮＡｓｙｎｔｈｅｓｉｓａｎｄｈｙｐｅｒｍｅｔａｂｏｌｉｓｍｏｆｎｕｃｌｅｉｃａｃｉｄ．γｇｌｕｔａｍｙｌｔｒａｎｓｆｅｒａｓｅ（ＧＧＴ，ＥＣ２．３．２．２...

关键词： liver neoplasms/enzymology γ glutamyl transferase/analysis disease models, animal RNA 2 fluoenyacetamide/analysis

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Photoinduced Dehalocyclization to Access Oxindoles Using Formate as a Reductant

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Chinese Journal of Chemistry 2024年第11期42卷 1203-1208页

作者： Wei Ge Jia-Xin Wang Ming-Chen fu yao fu Hefei National Research Center for Physical Sciences at the Microscale iChEMCAS Key Laboratory of Urban Pollutant ConversionAnhui Province Key Laboratory of Biomass Clean EnergyUniversity of Science and Technology of ChinaHefeiAnhui230026 China Anhui Province Key Laboratory of Value-Added Catalytic Conversion and Reaction Engineering Hefei University of TechnologyHefeiAnhui230009 China

Herein,we report an efficient and practical protocol for the photoinduced dehalocyclization of ortho-halophenylacrylamides with formate by the engagement of a CO_(2) radical anion to access substituted *** method proceeds smoothly under mild conditions and exhibits a wide range of substrate as well as remarkable functional group compatibility.

关键词： Formate CO_(2)Radical ions Oxindole Photocatalysts Cyclization Organohalides

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基因标志物在肝癌诊断中的临床价值

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中华肝脏病杂志 2007年第8期15卷 609-611页

作者：姚登福姜华南通大学附属医院 226001

肝癌的发生与肝炎病毒感染、致癌物作用、癌基因或癌相关基因激活、抗癌基因失活或胚胎期某些癌基因重新复活等诸多因素有关，并经启动、促进、演变多阶段的发病过程。临床匕肝癌确诊时已多属中、晚期，缺乏有效治疗，术后易复发及远处... 详细信息

肝癌的发生与肝炎病毒感染、致癌物作用、癌基因或癌相关基因激活、抗癌基因失活或胚胎期某些癌基因重新复活等诸多因素有关，并经启动、促进、演变多阶段的发病过程。临床匕肝癌确诊时已多属中、晚期，缺乏有效治疗，术后易复发及远处转移，预后较差，早诊断、早治疗至关重要。早期诊断和术后复发监测仍是临床研究的重点。

关键词：癌肝细胞基因标志诊断

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Characteristics of hepatic nuclear-transcription factor-kappa B expression and quantitative analysis in rat hepatocarcinogenesis

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Hepatobiliary & Pancreatic Diseases International 2009年第5期8卷 504-509页

作者： Wu, Wei yao, deng-fu Qiu, Li-Wei Sai, Wen-Li Shen, Jun-Jun Yu, Hong-Bo Wu, Xin-Hua Li, Yue-Ming Wang, Yi-Lang Gu, Wen-Jing Nantong Univ Affiliated Hosp Res Ctr Clin Mol Biol Nantong 226001 Peoples R China

BACKGROUND: Hepatocellular carcinoma (HCC) is one of the most common malignant tumors. We analyzed the expression of miclear-transcription factor-kappa B (NF-kappa B) during hepatocarcinogenesis in order to evaluate its dynamic expression and its clinical value in the development and diagnosis of HCC. METHODS: Hepatoma models were induced by oral administration of 2-acetamidoflurene (2-FAA) to male Sprague-Dawley rats. Morphological changes were observed after hematoxylin and eosin staining. The cellular distribution of NF-kappa B expression during different stages of cancer development was investigated by immunohistochemistry, and the level of NF-kappa B expression in liver tissues was quantitatively analyzed by ELISA. The gene fragments of hepatic NF-kappa B were amplified by nested-polymerase chain reaction assay. RESULTS: Hepatocytes showed vacuole-like degeneration during the early stages, then had a hyperplastic nodal appearance during the middle stages, and finally progressed to tubercles of cancerous nests with high differentiation. The NF-kappa B-positive material was buff-colored, fine particles localized in the nucleus, and the incidence of NF-kappa B-positive cells was 81.8% in degeneration, 83.3% in precancerous lesions, and 100% in cancerous tissues. All of these values were higher than those in controls (P<0.01). Hepatic NF-kappa B expression and hepatic NF-kappa B-mRNA were also higher during the course of HCC development (P<0.01). CONCLUSION: The NF-kappa B signal transduction pathway is activated during the early stages of HCC development, and its abnormal expression may be associated with the occurrence of HCC.

关键词： hepatocellular carcinoma nuclear factor-kappa B immunohistochemistry nested-polymerase chain reaction NF-kappa B-mRNA

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