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Biochemical properties of K_(11,48)-branched ubiquitin chains

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Chinese Chemical Letters 2018年第7期29卷 1155-1159页

作者： Lu-Jun Liang yanyan si Shan Tang Dongliang Huang Zhipeng A.Wang Changlin Tian Ji-Shen Zheng School of Life Sciences University of Science and Technology of China

As one of the most widely existing post-translational modification models, ubiquitination regulates diverse cellular activities. In eukaryotes, K-branched ubiquitin chains play key roles in cell cycle and protein quality control. However, the structural and biochemical properties of K-branched ubiquitin chains have not been well examined. Here we employed the synthetic linkage-and length-defined K-branched ubiquitin chains to examine their binding and hydrolysis properties in vitro. Quantitatively affinity determination of ubiquitin chains to the proteasome ubiquitin receptor S5 a indicated that the S5 a exhibited preference binding to K-branched chains over K-linked chains, but not K-conjugated chains. In addition, deubiquitination experiments were carried out and the results showed that K-branched chains were preferably hydrolyzed by proteasome-associated deubiquitinase Rpnll than homotypic Kor K-linked chains.

关键词： Post-translational modification Ubiquitination K_(11,48)-branched chains Affinity Deubiquitination Native chemical ligation of peptide hydrazine

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骨架环肽的化学合成研究进展

有机化学 2014年第3期34卷 450-460页

作者：司岩岩郭叶李海燕孙浩源方葛敏中国科学院合肥物质研究院强磁场科学中心合肥230031 清华大学化学系北京100084 辽宁大学生命科学院沈阳110036

骨架环肽是线性肽的C端和N端通过酰胺键进行首尾环合而形成的环状分子.研究者从细菌、真菌、植物和动物中发现了大量的骨架环肽.这种首尾环合的结构,使得骨架环肽具有很好的酶稳定性、热稳定性和化学稳定性,部分骨架环肽具有细胞膜通透... 详细信息

骨架环肽是线性肽的C端和N端通过酰胺键进行首尾环合而形成的环状分子.研究者从细菌、真菌、植物和动物中发现了大量的骨架环肽.这种首尾环合的结构,使得骨架环肽具有很好的酶稳定性、热稳定性和化学稳定性,部分骨架环肽具有细胞膜通透性.骨架环肽分子异常的稳定性和高效的生物活性,使得其成为目前药物领域的研究热点.为了更深入地研究它们的结构和功能,骨架环肽的制备成为一个重要问题.概述了化学合成骨架环肽的一些方法,包括:(1)固相环合策略;(2)液相环合策略;(3)分子内自然化学连接策略,并对这些方法的特点和效率进行了讨论比较.

关键词：骨架环肽环化自然化学连接

甲胎蛋白联合甲胎蛋白-L3、碱性磷酸酶和酸性水解酶检测在肝细胞癌诊断中的价值

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中国医药科学 2023年第13期13卷 140-144页

作者：司艳艳孙其静滨州医学院附属医院检验科山东滨州256600

目的探究甲胎蛋白(AFP)联合甲胎蛋白-L3(AFP-L3)、碱性磷酸酶(ALP)和酸性水解酶(AFU)检测在肝细胞癌(HCC)诊断中的价值。方法选取2019年1月至2020年4月滨州医学院附属医院收治的HCC患者98例作为HCC组,选取同时期的肝硬化患者98例作为肝... 详细信息

目的探究甲胎蛋白(AFP)联合甲胎蛋白-L3(AFP-L3)、碱性磷酸酶(ALP)和酸性水解酶(AFU)检测在肝细胞癌(HCC)诊断中的价值。方法选取2019年1月至2020年4月滨州医学院附属医院收治的HCC患者98例作为HCC组,选取同时期的肝硬化患者98例作为肝硬化组,同时期到本院进行健康检查的98名人员作为对照组。上述研究对象均接受AFP、AFP-L3、ALP和AFU指标检测,另外HCC组额外接受病理切片检查。结果HCC组的AFP、AFP-L3、ALP和AFU水平高于肝硬化组、对照组,差异有统计学意义(P<0.05),肝硬化组的各项指标水平高于对照组,差异有统计学意义(P<0.05);HCC组的AFP、AFP-L3、ALP和AFU指标单一和联合检测阳性率高于肝硬化组、对照组,差异有统计学意义(P<0.05),肝硬化组的各项指标单一和联合检测阳性率高于对照组,差异有统计学意义(P<0.05);联合指标检测的敏感性、准确性高于单一指标检测,差异有统计学意义(P<0.05);AFP、AFP-L3、ALP和AFU联合诊断的依从性高于病理切片检查,差异有统计学意义(P<0.05)。结论在HCC诊断中,应用AFP联合AFP-L3,ALP和AFU检测可以提高临床诊断准确性、敏感性,且具有无创性,可以较大程度上提高患者在诊断方面的依从性,为随后治疗工作的开展提供重要参考依据。

关键词：肝细胞癌甲胎蛋白甲胎蛋白-L3 碱性磷酸酶酸性水解酶

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妇科肿瘤治疗中铂类抗癌药物的应用研究

中国全科医学 2021年第S01期24卷 90-92页

作者：司秀文宋向芹燕晶晶陈洪娜司艳艳罗丽卿滨州医学院附属医院检验科山东省滨州市256600 滨州医学院附属医院血液科山东省滨州市256600

目的研究铂类抗癌药物应用至妇科肿瘤治疗当中的效果。方法选取时间为2018年1月—2019年1月期间本院收诊的100例妇科肿瘤患者,按照收诊的先后次序分组,其中50例妇科肿瘤患者给予紫杉醇+卡铂化疗方式治疗,将其划分至对比组,剩余50妇科肿... 详细信息

目的研究铂类抗癌药物应用至妇科肿瘤治疗当中的效果。方法选取时间为2018年1月—2019年1月期间本院收诊的100例妇科肿瘤患者,按照收诊的先后次序分组,其中50例妇科肿瘤患者给予紫杉醇+卡铂化疗方式治疗,将其划分至对比组,剩余50妇科肿瘤患者给予紫杉醇+顺铂化疗方式治疗,将其划入观察组,比较两组妇科肿瘤患者采用不同铂类抗癌药物化疗方治疗6个月后的疗效,同时详细阐述应用期间发生的不良反应。结果观察组治疗总效率为92.0%略高于对比组90.0%,两组治疗总效率比较差异无统计学意义(P>0.05);观察组总不良反应发生率18.0%略低于对比组20.0%,比较差异无统计学意义(P>0.05)。结论铂类抗癌药物应用至妇科肿瘤当中效果显著,临床应用前景广泛,对改善患者的病情,提升生活质量,改善其预后的意义重大,故值得临床借鉴和推广。

关键词：妇科肿瘤铂类抗癌药物疗效不良反应预后价值

Unraveling brain aging through the lens of oral microbiota

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Neural Regeneration Research 2025年第7期20卷 1930-1943页

作者： Qinchao Hu si Wang Weiqi Zhang Jing Qu Guang-Hui Liu State Key Laboratory of Membrane Biology Institute of ZoologyChinese Academy of SciencesBeijingChina Hospital of Stomatology Guanghua School of StomatologyGuangdong Provincial Key Laboratory of StomatologySun Yat-sen UniversityGuangzhouGuangdong ProvinceChina Advanced Innovation Center for Human Brain Protection and National Clinical Research Center for Geriatric Disorders Xuanwu HospitalCapital Medical UniversityBeijingChina Aging Translational Medicine Center International Center for Aging and CancerBeijing Municipal Geriatric Medical Research CenterXuanwu HospitalCapital Medical UniversityBeijingChina CAS Key Laboratory of Genomic and Precision Medicine Beijing Institute of GenomicsChinese Academy of Sciences and China National Center for BioinformationBeijingChina University of Chinese Academy of Sciences BeijingChina Institute for Stem Cell and Regeneration Chinese Academy of SciencesBeijingChina Aging Biomarker Consortium BeijingChina State Key Laboratory of Stem Cell and Reproductive Biology Institute of ZoologyChinese Academy of SciencesBeijingChina Beijing Institute for Stem Cell and Regenerative Medicine BeijingChina

The oral cavity is a complex physiological community encompassing a wide range of *** of oral microbiota can lead to various oral infectious diseases,such as periodontitis and tooth decay,and even affect systemic health,including brain aging and neurodegenerative *** studies have highlighted how oral microbes might be involved in brain aging and neurodegeneration,indicating potential avenues for intervention *** this review,we summarize clinical evidence demonstrating a link between oral microbes/oral infectious diseases and brain aging/neurodegenerative diseases,and dissect potential mechanisms by which oral microbes contribute to brain aging and *** also highlight advances in therapeutic development grounded in the realm of oral microbes,with the goal of advancing brain health and promoting healthy aging.

关键词： Alzheimer's disease brain aging multiple sclerosis neurodegeneration neurodegenerative diseases oral microbiota Parkinson's disease periodontitis bacteria Porphyromonas gingivalis

High Fe‑Loading single‑Atom Catalyst Boosts ROS Production by Density Effect for Efficient Antibacterial Therapy

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Nano-Micro Letters 2025年第2期17卷 187-203页

作者： si Chen Fang Huang Lijie Mao Zhimin Zhang Han Lin Qixin Yan Xiangyu Lu Jianlin Shi Department of Cardiology Shanghai Tenth People’s HospitalSchool of MedicineTongji UniversityShanghai 200092People’s Republic of China Shanghai Institute of Ceramics Chinese Academy of SciencesShanghai 200050People’s Republic of China Shanghai Frontiers Science Center of Nanocatalytic Medicine School of MedicineTongji UniversityShanghai 200092People’s Republic of China

The current single-atom catalysts(SACs)for medicine still suffer from the limited active site ***,we develop a synthetic method capable of increasing both the metal loading and mass-specific activity of SACs by exchanging zinc with *** constructed iron SACs(h^(3)-FNC)with a high metal loading of 6.27 wt%and an optimized adjacent Fe distance of~4 A exhibit excellent oxidase-like catalytic performance without significant activity decay after being stored for six months and promising antibacterial ***,a“density effect”has been found at a high-enough metal doping amount,at which individual active sites become close enough to interact with each other and alter the electronic structure,resulting in significantly boosted intrinsic activity of single-atomic iron sites in h^(3)-FNCs by 2.3 times compared to low-and medium-loading ***,the overall catalytic activity of h^(3)-FNC is highly improved,with mass activity and metal mass-specific activity that are,respectively,66 and 315 times higher than those of commercial Pt/*** addition,h^(3)-FNCs demonstrate efficiently enhanced capability in catalyzing oxygen reduction into superoxide anion(O_(2)·^(−))and glutathione(GSH)*** in vitro and in vivo assays demonstrate the superior antibacterial efficacy of h^(3)-FNCs in promoting wound *** work presents an intriguing activity-enhancement effect in catalysts and exhibits impressive therapeutic efficacy in combating bacterial infections.

关键词： Nanocatalytic medicine single-atom catalysts Reactive oxygen species(ROS) High metal loading Oxidase catalysis

NOX4 exacerbates Parkinson's disease pathology by promoting neuronal ferroptosis and neuroinflammation

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Neural Regeneration Research 2025年第7期20卷 2038-2052页

作者： Zhihao Lin Changzhou Ying Xiaoli si Naijia Xue Yi Liu Ran Zheng Ying Chen Jiali Pu Baorong Zhang Department of Neurology The Second Affiliated HospitalZhejiang University School of MedicineHangzhouZhejiang ProvinceChina

Parkinson's disease is primarily caused by the loss of dopaminergic neurons in the substantia nigra ***,a novel form of regulated cell death characterized by iron accumulation and lipid peroxidation,plays a vital role in the death of dopaminergic ***,the molecular mechanisms underlying ferroptosis in dopaminergic neurons have not yet been completely *** oxidase 4 is related to oxidative stress,however,whether it regulates dopaminergic neuronal ferroptosis remains *** aim of this study was to determine whether NADPH oxidase 4 is involved in dopaminergic neuronal ferroptosis,and if so,by what *** found that the transcriptional regulator activating transcription factor 3 increased NADPH oxidase 4 expression in dopaminergic neurons and astrocytes in an 1-methyl-4-phenyl-1,2,3,6 tetrahydropyridine-induced Parkinson's disease *** oxidase 4 inhibition improved the behavioral impairments observed in the Parkinson's disease model animals and reduced the death of dopaminergic ***,NADPH oxidase 4 inhibition reduced lipid peroxidation and iron accumulation in the substantia nigra of the Parkinson's disease model ***,we found that NADPH oxidase 4 interacted with activated protein kinase Cαto prevent ferroptosis of dopaminergic ***,by lowering the astrocytic lipocalin-2 expression,NADPH oxidase 4 inhibition reduced 1-methyl-4-phenyl-1,2,3,6 tetrahydropyridine-induced *** findings demonstrate that NADPH oxidase 4 promotes ferroptosis of dopaminergic neurons and neuroinflammation,which contribute to dopaminergic neuron death,suggesting that NADPH oxidase 4 is a possible therapeutic target for Parkinson's disease.

关键词： dopaminergic neuron ferroptosis NADPH oxidase 4(NOX4) neuroinflammation Parkinson's disease

Low-dimensional non-metal catalysts:principles for regulating p-orbital-dominated reactivity

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npj Computational Materials 2021年第1期7卷 1699-1732页

作者： si Zhou Wei Pei yanyan Zhao Xiaowei Yang Nanshu Liu Jijun Zhao Key Laboratory of Materials Modification by Laser Ion and Electron Beams(Dalian University of Technology)Ministry of EducationDalian 116024China

Activation of p-block elements to replace the rare and precious transition metals for renewable energy applications is highly *** this review,we go over recent experimental and theoretical progress on the low-dimensional non-metal materials for clean energy production,including carbon,silicon,oxide,boron,and phosphorus-based nanostructures,with the p-block elements serving as active *** aim to elucidate the mechanism for triggering activity in different kinds of non-metal systems,and extract general principles for controlling the p-orbital-mediated reactivity from a theoretical point of *** perspectives and challenges for developing high-efficiency non-metal catalysts are provided in the end.

关键词： reactivity orbital principles

阿列克谢耶夫《聊斋志异》译本的译者行为批评分析

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外语学刊 2025年第1期 92-98页

作者：雷丽斯黑龙江大学高级翻译学院哈尔滨150080

《聊斋志异》是我国古代短篇小说的艺术丰碑,由苏联汉学家阿列克谢耶夫完成的俄语选译本在俄罗斯完成经典化。本文以阿列克谢耶夫译本为例,运用文本细读法,从翻译内外二分化视野,对译者行为进行批评分析。研究发现,在翻译外层面上,译者... 详细信息

《聊斋志异》是我国古代短篇小说的艺术丰碑,由苏联汉学家阿列克谢耶夫完成的俄语选译本在俄罗斯完成经典化。本文以阿列克谢耶夫译本为例,运用文本细读法,从翻译内外二分化视野,对译者行为进行批评分析。研究发现,在翻译外层面上,译者身份、译者与作者之间的“知己关系”等因素促使译者行为偏向作者/原文一端;在翻译内层面上,译者运用多种翻译手段最大限度地实现了对作者/原文的求真,同时在求真的基础上,使译文对读者/社会务实,译者行为合理度较佳。

关键词：译者行为批评《聊斋志异》阿列克谢耶夫译者行为典籍翻译