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Development and drought tolerance assay of marker-free transgenic rice with OsAPX2 using biolistic particle-mediated co-transformation

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The Crop Journal 2017年第4期5卷 271-281页

作者： Dan Feng yanwei wang Jinxia Wu Tiegang Lu Zhiguo Zhang Biotechnology Research Institute/National Key Facility for Crop Gene Resources and Genetic Improvement Chinese Academy of Agricultural Sciences Beijing 100081 China

Abiotic stresses such as drought, salinity, and low temperature cause–losses in rice production worldwide. The emergence of transgenic technology has enabled improvements in the drought resistance of rice plants and helped avert crop damage due to drought *** marker genes conferring resistance to antibiotics or herbicides have been widely used to identify genetically modified plants. However, the use of such markers has limited the public acceptance of genetically modified organisms. Marker-free materials (i.e., those containing a single foreign gene) may be more easily accepted by the public and more likely to find common use. In the present study, we created marker-free drought-tolerant transgenic rice plants using particle bombardment. Overall, 842 T_0plants overexpressing the rice ascorbate peroxidase-coding gene OsAPX2 were generated. Eight independentmarker-free lines were identified from T_1 seedlings using the polymerase chain *** molecular characteristics of these lines were examined, including the expression level,copy number, and flanking sequences of OsAPX2, in the T_2 progeny. A simulated drought test using polyethylene glycol and a drought-tolerance test of seedlings confirmed that the marker-free lines carrying OsAPX2 showed significantly improved drought tolerance in seedlings. In the field, the yield of the wild-type plant decreased by 60% under drought conditions compared with normal conditions. However, the transgenic line showed a yield loss of approximately 26%. The results demonstrated that marker-free transgenic lines significantly improved grain yield under drought-stressed conditions.

关键词： Rice Particle bombardment-mediated co-transformation Marker-free Drought-tolerance

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Selective nickel-electrocatalyzed benzylic C-H oxygenation of functionalized alkyl arenes

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Chinese Chemical Letters 2024年第2期35卷 396-401页

作者： Shunyao Tang Siyi wang Dongmei Zhang Xinxing Zhang Guang Yang yanwei wang Youai Qiu State Key Laboratory of Elemento-Organic Chemistry Frontiers Science Center for New Organic MatterCollege of ChemistryNankai UniversityTianjin 300071China College of Chemistry Key Laboratory of Advanced Energy Materials Chemistry (Ministry of Education)Renewable Energy Conversion and Storage Center (RECAST)Frontiers Science Center for New Organic MatterNankai UniversityTianjin 300071China

Herein, a site-selective paired electrochemical C–H oxidation of functionalized alkyl arenes promoted by nickel catalyst is disclosed. A Ni(Ⅱ)-dioxygen species formed in situ efficiently enable the oxidation process under mild conditions with a broad substrate scope with excellent functional group compatibilities,such as free carboxylic acid, aldehyde, halogen(including aryl iodide), amide and amino acid. The use of the nickel catalyst in combination with water provides a safe, green and economical method for oxidation of a range of molecules varying in complexity and drug derivatives, demonstrating its potential application in organic synthesis and the pharmaceutical industry. Reaction outcomes and mechanistic studies revealed the key role of the in situ Ni(Ⅱ)-dioxygen species for the subsequent oxidation of C(sp^(3))–H bonds,and short-lived reactive intermediates(aryl radical cation) was rapidly captured by the combination of a bipolar ultramicroelectrode(BUME) with nano-electrospray ionization mass spectrometry.

关键词： Electrochemical C-H oxidation Functionalized alkyl arenes Paired nickel-electrocatalysis H_(2)O oxidation Ketones

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Modification on the Performance of the Hemihydrate Gypsum with the Plant Source Polymer of Dry Matcha Powder

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Journal of Wuhan University of Technology(Materials Science) 2018年第6期33卷 1452-1458页

作者： LI Haoxin wang yanwei Key Laboratory of Advanced Civil Engineering Materials Ministry of Education Tongji University China Railway No.9 Engineering Testing Co. Ltd

The objective of this study was to assess the feasibility of using the plant-source polymer of the matcha powder as a composite admixture for hemihydrate gypsum. Hemihydrate gypsum was mixed with different contents of matcha powder, and then the water requirement for the normal consistency, setting times, density, strength, hydration and microstructure of the hardened mixture were evaluated. The experimental results showed that it increased the water requirement for the normal consistency, and it regulated the setting times and reduced the density. Hemihydrate gypsum with more matcha powder had the higher water requirement, longer setting times and lower density. Less than 1% matcha powder had slight impact on the strength of hardened paste, but more than 1% matcha powder had a remarkable one. Matcha powder changed the hydration process and prolonged the induction and acceleration period. Small needlelike crystals were transformed into longer, larger and thicker ones as more matcha powder was mixed. This case is closely related to the prolongation of the induction and acceleration period. Besides, more and larger pores were observed in the hardened paste with more matcha powder. It is attributed to the appearances of the tea polyphenol in matcha powder and the larger and longer crystal morphology in hardened paste as well as the high water requirement for the normal consistency. These results are important to the application of matcha powder as a composite admixture for the hemihydrate gypsum as well as the prosperity and development of the tea industry.

关键词： matcha powder hemihydrate gypsum strength hydration property microstructure

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The RNA Editing Factor WSP1 Is Essentia for Chloroplast Development in Rice

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Molecular Plant 2017年第1期10卷 86-98页

作者： Zhiguo Zhang Xuean Cui yanwei wang JinxiaWu Xiaofeng Gu Tiegang Lu Biotechnology Research Institute/National Key Facility for Genetic Resources and Gene Improvement The Chinese Academy of Agricultural Sciences Beijing 100081 P. R China These authors contributed equally to this article

Although the multiple organellar RNA editing factors （MORFs） in the plastids of Arabidopsis thaliana have been extensively studied, molecular details underlying how MORFs affect plant development in other species, particularly in rice, remain largely unknown. Here we describe the characterization of wspl, a rice mutant with white-stripe leaves and panicles. Notably, wspl exhibited nearly white immature panicles at the heading stage. Transmission electron microscopy analysis and chlorophyll content measurement re- veale i a chloroplast developmental defect and reduced chlorophyll accumulation in wspl. Positional cloning of WSP1 found a point mutation in OsO4g51280, whose putative product shares high sequence similarity with MORF proteins. Complementation experiments demonstrated that WSP1 was responsible for the variegated phenotypes of wspl. WSP1 is localized to chloroplasts and the point mutation in wspl affected the editing of multiple organellar RNA sites. Owing to the defect in plastid RNA editing, chloroplast ribosome biogenesis and ndhA splicing were also impaired in wspl, which may affect normal chloroplast development in the leaves and panicles at the heading stage. Together, our results demonstrate the importance of rice WSP1 protein in chloroplast development and broaden our knowledge about MORF family members in rice.

关键词： rice RNA editing PEP complex and ribosome biosynthesis chloroplast development

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Low Li ion diffusion barrier on low-crystalline FeOOH nanosheets and high performance of energy storage

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Nano Research 2020年第3期13卷 759-767页

作者： Jien Li Shuang Luo Congcong wang Qian Tang yanwei wang Xiangyu Han Hao Ran Jing Wan Xiao Gu Xue wang Chenguo Hu Department of Applied Physics State Key Laboratory of Power Transmission Equipment&System Security and New TechnologyChongqing UniversityChongqing400044China Faculty of Science Ningbo UniversityNingbo315211China

To obtain symmetric supercapacitors(SCs)with high energy density,it is critical to fabricate an electrode with wide potential window and excellent capacitive ***,by using the strong double hydrolysis reaction between anions and cations,the FeOOH nanosheets on the surface of activated carbon cloth(FeOOH@AC)are prepared through a simple hydrothermal *** FeOOH@AC electrode exhibits maximum capacitance of 4,090 mF·cm^−2 at wider potential window-1–0 V and 3,250 mF·cm^−2 at 0–1 V versus SCE in 2 M LiNO3 *** two pieces of FeOOH@AC electrodes the obtained symmetric SC can operate at the voltage window of 2 *** FeOOH symmetric SC shows high energy density of 13.261 mWh·cm^−3 at a power density of 14.824 mW·cm^−3 and maintains 4.175 mWh·cm^−3 at a maximum power density of 118.564 mW·cm^−3,as well as excellent charge storage capacity and cyclic *** ion adsorption and diffusion mechanism on the(200)facets of FeOOH are explained by the density functional theory(DFT)*** simple synthesis process and excellent capacitance performance of the FeOOH@AC composite make it a very promising candidate for high performance symmetric SC electrodes.

关键词： low-crystalline nanosheets FeOOH density functional theory supercapacitor

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The path,value and limits of the Confucius Institute in carrying out public diplomacy

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Economic and Political Studies 2021年第2期9卷 217-229页

作者： yanwei wang Chinenye Gerlof Uzodinma Caoyuan Niu School of International Education Shandong UniversityJi’nanChina Centre of Confucius Institute Studies Shandong UniversityJi’nanChina School of African Studies Beijing Foreign Studies UniversityBeijingChina

On the basis of Chinese language teaching and cultural communication activities,the Confucius Institute carries out a variety of cultural exchange activities between China and the host country,which has become an effective way for China to carry out public diplomacy and participate in global cultural *** analysing the concepts of public diplomacy,cultural diplomacy,people-to-people exchange and cultural governance,this paper summarises three typical paths of Confucius Institutes in carrying out public diplomacy:‘from the government to foreign nationals’,‘from the government to Chinese citizens and then to foreign nationals’,and‘from the government to foreign nationals and then to foreign nationals’.The value of the Confucius Institute in carrying out public diplomacy is reflected on the level of national diplomacy,international exchange and global ***,the capability of the Confucius Institute as a language and cultural communication institution initiated by China and in cooperation with other countries in carrying out public diplomacy is *** limitation,on one hand,originates from the nature of the Confucius Institute as a language and cultural communication organisation,and on the other hand,is related to the difficulties and challenges that China faces in its participation in global *** diplomacy and cultural diplomacy are based on national *** also transcend national interests and,furthermore,become a way for a country to actively participate in global cultural *** experience of and challenges faced by the Confucius Institute can have important implications for China in participating in global cultural governance.

关键词： Global governance global cultural governance public diplomacy cultural diplomacy Confucius Institute

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Fabrication of the 19-filament Fe/Cu clad MgB_2 wire via in situ powder-in-tube method

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Chinese Science Bulletin 2007年第19期52卷 2621-2625页

作者： YU ZhengGuang MA yanwei wang DongLiang GAO ZhaoShun ZHANG XianPing Applied Superconductivity Laboratory Institute of Electrical Engineering Chinese Academy of Sciences Beijing 100080 China

Using commercial amorphous B powder (92% in purity) and Mg powder (99% in purity) as starting materials, 19-filament Fe/Cu clad MgB2 wires were fabricated by an in situ powder-in-tube method. Heat treatment was performed at 700℃ for 1 h under an argon gas atmosphere. The influence of Mg/B ratio on the microstructure and superconducting properties of the wires was investigated. It was found that the major phases of MgB2 wires were MgB2 accompanied with relatively small amounts of MgO and Fe2B impurities. With 5% excess Mg addition, the onset TC slightly decreased. However, the transport JC at 4.2 K and 4 T reached 1.07×104 A·cm-2, increasing by a factor of 1.4 compared to the stoichiometric sample. Moreover, the Mg1.05B2 sample showed an improved field dependence of JC, suggesting that less voids and smaller grain size of the Mg1.05B2 core lead to better grain connectivity and stronger flux pinning.

关键词： MgB2金属导线铜铁复合物制造工业灯丝

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Differentiation of fetal pancreatic stem cells into neuron-like and islet-like cells in vitro

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Neural Regeneration Research 2012年第7期7卷 506-510页

作者： Xiufeng Hua yanwei wang Peiwen Lian Shouxin Zhang Jianyuan Li Haiyan wang Shulin Chen Wei Gao Department of Endocrinology Yuhuangding Hospital Yantai 264000 Shandong Province China Research Center of Stem Cell Engineering of Shandong Central Laboratory of Yuhuangding Hospital Yantai 264000 Shandong ProvinceChina

Pancreatic stem cells were isolated and cultured from aborted human fetal pancreases of gestational age 14-20 weeks. They were seeded at a density of 1 × 104 in serum-free media for differentiation into neuron-like cells, expressing β-tubulin III and glial fibrillary acidic protein. These neuron-like cells displayed a synapse-like morphology and appeared to form a neuronal network. Pancreatic stem cells were also seeded at a density of 1 × 105 for differentiation into islet-like cells, expressing insulin and glucagon, with an islet-like morphology. These cells had glucose-stimulated secretion of human insulin and C-peptide. Results suggest that pancreatic stem cells can be differentiated into neuron-like and islet-like cells.

关键词： fetal pancreas pancreatic stem cells differentiation islet-like cells neuron-like cells neural regeneration

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CO flux of soil respiration in natural recovering karst abandoned farmland in Southwest China

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Acta Geochimica 2020年第4期39卷 527-538页

作者： yanwei wang Weijun Luo Guangneng Zeng Hanling Yang Meifang wang Yina Lyu Anyun Cheng Lin Zhang Xianli Cai Jia Chen Shijie wang State Key Laboratory of Environmental Geochemistry Institute of GeochemistryChinese Academy of Sciences99 West Lincheng Road.Guiyang 550081Guizhou ProvinceChina University of Chinese Academy of Sciences Beijing 100049China Puding Karst Ecosystem Research Station Chinese Academy of SciencesPuding 562100China School of Chemistry and Eco-Environniental Engineering Guizhou Minzu UniversityGuiyang 550025China School of Geography and Environmental Sciences Guizhou Normal UniversityGuiyang 550025China

To estimate carbon sequestration potential in the karst area,soil respiration in a natural recovering karst abandoned farmland in Shawan,Puding,Guizhou,southwest China was continuously and automatically monitored for more than two *** results show that the CO2flux of soil respiration(2.63±1.89 lmol m^-2s-^1)is higher in the karst area than in non-karst areas under similar conditions but that regional value(1.32 lmol m-2s-1)is lower because of larger rock fragment coverage(~50%).A the same time,the temperature sensitivity of soil respiration(Q10)in this study area is significantly higher than that of non-karst areas under similar *** respiration has an obvious temporal variation,which is reflected in a significant exponential relationship between soil respiration and soil temperature,but the relationship between soil respiration and soil moisture is very *** soil respiration has an obvious spatial variation,which is likely affected by different diffusion or water-rock reaction processes.

关键词： Karst critical zone Abandoned land Soil respiration Carbon cycle

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RHOMBUS THINKING METHOD AND ITS APPLICATION IN SCHEME DESIGN

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Chinese Journal of Mechanical Engineering 2001年第2期14卷 156-159页

作者： Zhao yanwei wang Wanliang Science Research Center,Zhejiang University of Technology Zhang Guoxian Shanghai University 浙江工业大学科学研究中心浙江杭州上海大学上海

Rhombus thinking, a new creative thinking method,is the combination of divergent thinking process and convergent thinking process,in which qualitative analysis is carried out before quantitative analysis This method tries to solve the bottle neck problem in intelligent CAD based on the extension theory The rhombus thinking method to the scheme design of new products is applied In this process, firstly, the matter element expression for the know information are set up, and then a set of matter elements are opened up by matter elements extension method; Finally,the useful information are got by appraisal method of dependent degree It has been successfully applied to the scheme design for the cutter store of machining center Theoretical and experimental results demonstrated fhat the method is much more accurate,objective and efficient than the traditional one

关键词： Extension theory Rhombus thinking method Scheme design Artificial Intelligence

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