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Multifaceted regulatory functions of CsBPC2 in cucumber under salt stress conditions

Horticulture Research 2023年第5期10卷 126-138页

作者： Shuzhen li Mintao Sun li Miao Qinghua Di lijun Lv Xianchang Yu Yan Yan Chaoxing He Jun Wang Aokun Shi yansu li State Key Laboratory of Vegetable Biobreeding Institute of Vegetables and FlowersChinese Academy of Agricultural SciencesBeijing 100081China Ganzhou Key Laboratory of Greenhouse Vegetable College of Life ScienceGannan Normal UniversityGanzhou 341000China Collaborative Innovation Center for Efficient and Green Production of Agriculture in Mountainous Areas of Zhejiang Province College of Horticulture ScienceZhejiang Agriculture and Forestry UniversityHangzhou 311300ZhejiangChina

BASIC PENTACYSTEINE(BPC)transcription factors are essential regulators of plant growth and ***,BPC functions and the related molecular mechanisms during cucumber(Cucumis sativus L.)responses to abiotic stresses,especially salt stress,remain *** previously determined that salt stress induces CsBPC expression in *** this study,Csbpc2 transgene-free cucumber plants were created using a CRISPR/Cas9-mediated editing system to explore CsBPC functions associated with the salt stress *** Csbpc2 mutants had a hypersensitive phenotype,with increased leaf chlorosis,decreased biomass,and increased malondialdehyde and electrolytic leakage levels under salt stress ***,a mutated CsBPC2 resulted in decreased proline and soluble sugar contents and antioxidant enzyme activities,which led to the accumulation of hydrogen peroxide and superoxide ***,the mutation to CsBPC2 inhibited salinity-induced PM-H+-ATPase and V-H+-ATPase activities,resulting in decreased Na+efflux and increased K+*** findings suggest that CsBPC2 may mediate plant salt stress resistance through its effects on osmoregulation,reactive oxygen species scavenging,and ion homeostasis-related regulatory ***,CsBPC2 also affected ABA *** mutation to CsBPC2 adversely affected salt-induced ABA biosynthesis and the expression of ABA signaling-related *** results indicate that CsBPC2 may enhance the cucumber response to salt *** may also function as an important regulator of ABA biosynthesis and signal *** findings will enrich our understanding of the biological functions of BPCs,especially their roles in abiotic stress responses,thereby providing the theoretical basis for improving crop salt tolerance.

关键词： CRISPR/Cas9 stress functions

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Interference of CsGPA1,theα-submit of G protein,reduces drought tolerance in cucumber seedlings

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Horticultural Plant Journal 2021年第3期7卷 209-220页

作者： Yumei liu Qinghua Du Longqiang Bai Mintao Sun yansu li Chaoxing He Jun Wang Xianchang Yu Yan Yan The Institute of Vegetables and Flowers Chinese Academy of Agricultural SciencesBeijing 100081China College of Agricultural and Biological Engineering Heze UniversityHezeShandong 274000China Tianjin Agricultural University Tianjin 300384China School of Horticulture Shanxi Agricultural UniversityTaiguShanxi 030801China

The G proteinα-subunit,GPA1,is an integral component of several signaling pathways in plants,including response to abiotic ***,the molecular mechanism behind these processes remains largely unknown in the cucumber plant(Cucumis sativus L.).In order to further understand the role of CsGPA1 in cucumber under drought stress,changes in plant growth,physiological parameters,and gene expression of CsAQPs were all measured under water stress induced by polyethylene glycol(PEG)using wild type(WT)and CsGPA1-interference(RNAi)cucumber *** results demonstrated that the RNAi plants had lower drought tolerance,displaying seriously withered leaves,lower relative growth rate,lower root-shoot ratio,and lower root activity under drought stress compared to WT *** studies indicated that the suppression of CsGPA1 weakened drought stress tolerance due to higherwater loss rate in the leaves,higher levels of hydrogen peroxide(H2O2),increased malondialdehyde(MDA)content,lower free proline content,lower soluble sugar content,lower soluble protein content,and decreased antioxidant enzyme ***-PCR analysis demonstrated that the interference of CsGPA1 up-regulated the expression of most AQP genes(except for CsPIP2;3 in leaves)and down-regulated the expression of CsPIP1;2,CsPIP1;4,CsPIP2;1,and CsPIP2;4 in roots under drought stress when compared to WT *** results demonstrated that CsGPA1 could function as a positive regulator in drought stress response by decreasing the accumulation of reactive oxygen species(ROS),improving permeable potentials,and reducing water loss in cucumber plants.

关键词： Cucumis sativus CsGPA1 Drought tolerance ROS scavenging Water content

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Sugars promote graft union development in the heterograft of cucumber onto pumpkin

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Horticulture Research 2021年第1期8卷 2198-2214页

作者： li Miao Qing li Tian-shu Sun Sen Chai Changlin Wang Longqiang Bai Mintao Sun yansu li Xing Qin Zhonghua Zhang Xianchang Yu Institute of Vegetables and Flowers Chinese Academy of Agriculture SciencesBeijing 100081China College of Horticulture Qingdao Agricultural UniversityQingdao 266109China College of Horticulture Shanxi Agricultural UniversityTaiguJinzhongShanxi 030801China

The use of heterografts is widely applied for the production of several important commercial crops,but the molecular mechanism of graft union formation remains poorly ***,cucumber grafted onto pumpkin was used to study graft union development,and genome-wide tempo-spatial gene expression at the graft interface was comprehensively *** analysis suggested that resumption of the rootstock growth occurred after both phloem and xylem reconnection,and the scion showed evident callus production compared with the rootstock 3 days after ***,transcriptome data revealed specific responses between the scion and rootstock in the expression of genes related to cambium development,the cell cycle,and sugar metabolism during both vascular reconnection and healing,indicating distinct ***,lower levels of sugars and significantly changed sugar enzyme activities at the graft junction were observed during vascular ***,we found that the healing process of grafted etiolated seedlings was significantly delayed,and graft success,xylem reconnection,and the growth of grafted plants were enhanced by exogenous *** demonstrates that graft union formation requires the correct sugar ***,we also found that graft union formation was delayed with a lower energy charge by the target of rapamycin(TOR)inhibitor AZD-8055,and xylem reconnection and the growth of grafted plants were enhanced under AZD-8055 with exogenous glucose *** together,our results reveal that sugars play a positive role in graft union formation by promoting the growth of cucumber/pumpkin and provide useful information for understanding graft union healing and the application of heterografting in the future.

关键词： healing metabolism union

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Alterations in Ascorbic Acid Levels by Transformation of the L-galactono-1,4-lactone Dehydrogenase Gene in Solanum tuberosum

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Horticultural Plant Journal 2015年第2期1卷 77-85页

作者： DONG Yumei li yansu JIAO Zigao li Chaohan YU Xianchang The Institute of Vegetables and Flowers Chinese Academy of Agricultural Sciences Vegetable and Flower Research Institute Shandong Academy of Agricultural Sciences Key Laboratory for Biology of Greenhouse Vegetable of Shandong Province National Center for Vegetable Improvement (Shandong)

L-galactono-1, 4-lactone dehydrogenase(GLDH) is an important enzyme that catalyzes the last step of the ascorbate biosynthetic pathways in plants. A full-length c DNA clone encoding GLDH was isolated from potato(Solanum tuberosum L. ‘Favorita') leaf and subcloned into a binary vector, p BI121, to construct sense and antisense recombinant plant expression vectors. The recombinants were introduced into potato via Agrobacterium-mediated transformation, and plants were confirmed as transgenic using PCR and quantitative real-time PCR. Two anti-sense potato lines(G1 and G2) and three sense lines(G3, G4 and G5) were obtained. The GLDH activity of G4 and G5 were increased in vivo. Moreover, the ascorbic acid(As A) and dehydroascorbate(DHA) contents were up-regulated in both leaves and tubers. However, the shoots of G1 were suppressed and its leaves were deformed. Additionally, the As A contents in G1 leaves and tubers decreased by 28.8% and 10.3%, respectively. The GLDH activity in leaves treated with L-galactono-1,4- lactone(L-Gal L) increased in all lines, as did the As A and DHA contents. These results indicate that GLDH activity plays an important role in regulating the As A level as well as the growth and development of potato plants.

关键词： Solanum tuberosum ascorbic acid L-galactono-1 4-lactone dehydrogenase(GLDH) genetic transformation

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Dynamic Expression of mi RNAs and Their Targets in the Response to Drought Stress of Grafted Cucumber Seedlings

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Horticultural Plant Journal 2016年第1期2卷 41-49页

作者： li Chaohan li yansu BAI Longqiang HE Chaoxing YU Xianchang Institute of Vegetables and Flowers Chinese Academy of Agricultural Sciences Horticultural Research Institute Shanghai Academy of Agricultural Sciences

Grafting of cucumber is widely used to improve growth and tolerance to biotic and abiotic stresses. Micro RNAs(miRNAs) regulate plant growth and development, and respond to various stresses through negative, post-transcriptional regulation of the expression of their target genes. We grafted cucumber(Cucumis sativus) scions onto pumpkin(Cucurbita moschata) rootstocks to study the molecular mechanisms of miRNA-mediated graftinginduced responses to drought stress. The relative expressions of 17 selected miRNAs and their predicted target m RNAs were detected by quantitative real-time PCR(q RT-PCR) in leaves and roots of hetero-grafted cucumber seedlings(cucumber as scion and pumpkin as rootstock) and autografted cucumber seedlings(cucumber as scion and rootstock) after 24 hours of 15% PEG6000 treatment. Compared with the expression in leaves of auto-grafted cucumber seedlings, the expressions of most miRNAs in the leaves of hetero-grafted seedlings changed dynamically: induced under normal conditions, and reduced after 3 h of drought stress, and then induced after 8 h and 24 h of drought stress. Similarly, compared with the expression in roots of auto-grafted cucumber seedlings, the expressions of most miRNAs in the roots of hetero-grafted cucumber seedlings changed dynamically: reduced under normal condition, and still reduced after 1 h, but induced after 3 h and 8 h, then reduced significant y after24 h of drought stress. These results are useful for the functional analysis of miRNAs in the mediation of grafting-dependent drought tolerance.

关键词： cucumber graft drought miRNA target

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适于可压缩多尺度流动的紧致型激波捕捉格式

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北京航空航天大学学报 2017年第8期43卷 1602-1609页

作者：李彦苏阎超于剑北京航空航天大学航空科学与工程学院北京100083

针对可压缩多尺度流动数值模拟特点,研究一种五阶高分辨率紧致型激波捕捉格式——紧致重构加权基本无振荡(CRWENO)格式。该格式利用非线性权系数将低阶紧致格式加权组合以达到高阶精度。在光滑区域蜕化成具有高分辨率的五阶线性紧致格式... 详细信息

针对可压缩多尺度流动数值模拟特点,研究一种五阶高分辨率紧致型激波捕捉格式——紧致重构加权基本无振荡(CRWENO)格式。该格式利用非线性权系数将低阶紧致格式加权组合以达到高阶精度。在光滑区域蜕化成具有高分辨率的五阶线性紧致格式,在间断附近则能保持计算稳定无振荡。对CRWENO格式、目前广泛使用的加权基本无振荡(WENO)格式及两格式对应的线性格式(即五阶线性迎风格式和五阶紧致格式)进行数值性能研究,评估非线性权系数对格式耗散及频谱特性的影响。使用一维、二维、三维典型算例进行数值试验,探讨线性/非线性、紧致/非紧致格式在可压缩多尺度流动模拟中的优势和不足。结果表明,CRWENO格式在强压缩性流场模拟中能够稳定地捕捉激波,其紧致特性则改善了非线性格式普遍存在的耗散过大、分辨率较差的问题,使其能够清晰捕捉多尺度流动结构。因此,该格式在可压缩多尺度流动模拟中具有较大优势。

关键词：紧致格式加权基本无振荡(WENO)格式激波捕捉数值耗散频谱特性可压缩多尺度流动

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三阶中心无振荡格式性能分析

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北京航空航天大学学报 2014年第11期40卷 1576-1580页

作者：李彦苏阎超屈峰于剑北京航空航天大学航空科学与工程学院北京100191

三阶中心无振荡格式Cn3(Centered nonoscillatory scheme of third order)使用对称模板构造具有三阶精度的插值公式.利用单调区域与精确区域修正原始插值参数,该格式能够获得间断附近无振荡、光滑区域高精度的计算结果.通过一维和二维... 详细信息

三阶中心无振荡格式Cn3(Centered nonoscillatory scheme of third order)使用对称模板构造具有三阶精度的插值公式.利用单调区域与精确区域修正原始插值参数,该格式能够获得间断附近无振荡、光滑区域高精度的计算结果.通过一维和二维典型算例,将Cn3格式与三阶和五阶WENO格式(Weighted Essentially Non-Oscillatory schemes)进行比较,重点分析了3种格式的间断分辨率、计算稳定性和数值耗散性.分析可见,Cn3格式能够精确、稳定地捕捉激波和接触间断,同时对光滑流动区域的小尺度流动结构保持较低的耗散,值得进一步研究及推广应用.

关键词：高精度格式 WENO格式间断分辨率稳定性数值耗散性

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Effects of Two Kinds of Arbuscular Mycorrhizal Fungi (AMF) Inoculums on Cucumber Seedlings Growth

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Plant Diseases and Pests 2016年第5期7卷 36-38页

作者： Sun Xiuxiu Jin Wenjuan li yansu Yan Yan Yu Xianchang He Chaoxing Institute of Vegetables and Flowers Chinese Academy of Agricultural Sciences

To study the effects of AMF （ arbuscular mycorrhizal fungi） ineeulums on seedling growth of cucumber, vegetable seeds were inoculated with two kinds of AMF ineculums BF （ mycorrhiza for plants） and VT （ mycorrhiza for vegetables） from Czech Republic during sowing in trays in greenhouse. The root AMF infection rates in seedlings were 49.30% and 37.65% respectively after 35 d growth. The plant height, stem diameter, fresh and dry weight of those seedlings inoculated with BF and VT were higher than that in CK. Compared with the CK, VT inoculation significantly improved the root activity, chlorophyll content and photosynthetic rate of cucumber seedlings. Comprehensive analysis showed that VT could promote seedling growth of protected cucumber.

关键词： Cucumber Arbuscular mycon'hizal fungi (AMF) ineculum Seedling growth Photosynthetic physiology

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低速修正的可压缩求解器对湍流模拟精度的影响

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北京航空航天大学学报 2019年第11期45卷 2199-2206页

作者：李彦苏张坤何承军阎超北京机电工程研究所北京100074 北京航空航天大学航空科学与工程学院北京100083

修正可压缩求解器能提高其对高速湍流中低速区的模拟精度,但低速修正效果受到求解器、计算格式精度、网格量等多因素影响,难以直接评估。研究了不同阶数、分辨率、网格量下,有无低速修正的可压缩求解器对复杂湍流模拟的影响。通过泰勒-... 详细信息

修正可压缩求解器能提高其对高速湍流中低速区的模拟精度,但低速修正效果受到求解器、计算格式精度、网格量等多因素影响,难以直接评估。研究了不同阶数、分辨率、网格量下,有无低速修正的可压缩求解器对复杂湍流模拟的影响。通过泰勒-格林涡算例,定量分析了不同结果的差异。结果表明:不同网格量、计算方法组合下,低速修正对结果的影响不同。网格量较小、重构格式精度较低的情况下,低速修正方法能够有效提高计算精度。

关键词：可压缩湍流低速修正高精度格式全速域格式泰勒-格林涡

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实际控制人缺失与内部人机会主义减持

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证券市场导报 2024年第2期 22-32页

作者：田昆儒李颜苏薛坤坤天津财经大学会计学院天津300222 郑州大学商学院河南郑州450001

当前,实际控制人缺失的现象愈发普遍,但关于无实际控制人引发的公司治理问题的研究较少。本文以2012—2021年沪深A股非金融上市公司为样本,从监督和信息优势角度实证考察了无实际控制人对内部人机会主义减持的影响。研究发现,无实际控... 详细信息

当前,实际控制人缺失的现象愈发普遍,但关于无实际控制人引发的公司治理问题的研究较少。本文以2012—2021年沪深A股非金融上市公司为样本,从监督和信息优势角度实证考察了无实际控制人对内部人机会主义减持的影响。研究发现,无实际控制人与内部人机会主义减持行为显著正相关;机制检验表明,公司实际控制人缺失会削弱对内部人行为的监督、降低信息透明度从而影响内部人机会主义减持。进一步地,公司治理水平的提高和外部法治环境的改善,可以缓解无实际控制人状态下内部人的机会主义减持行为,但当无实际控制人企业存在控股股东时,内部人机会主义减持次数更高。本文有助于强化对内部人机会主义减持行为的监督,也为完善无实际控制人监管提供了新思路。

关键词：实际控制人机会主义减持代理问题信息透明度

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