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Antioxidant properties of magnesium lithospermate B contribute to the cardioprotection against myocardial ischemia/reperfusion injury in vivo and in vitro

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Journal of Traditional Chinese Medicine 2013年第1期33卷 85-91页

作者： Wei Quan Ying Yin Miaomiao Xi Dan Zhou yanrong zhu Yue Guan Chao Guo Yanhua Wang Jialin Duan Aidong Wen Department of Pharmacy Xijing HospitalFourth Military Medical University

OBJECTIVE： To determine the cardioprotective ef- fect of magnesium lithospermate B （MLB） on myo- cardial ischemia/reperfusion （MI/R） injury and to in- vestigate the antioxidant potential in vivo and in vitro. METHODS： MI/R injury was induced by the occlu- sion of left anterior descending coronary artery for 30 min followed by reperfusion for 3 h in rats. After reperfusion, hearts were harvested to assess infarct size, histopathological damages, the levels of superoxide dismutase （SOD）, catalase （CAT）, glutathione peroxidase （GPx）, reduced glutathione （GSH） and malondialdehyde （MDA）. Blood samples were collected to determine serum levels of creatine kinase-MB （CK-MB）, cardiac troponin （cTnl） and lactate dehydrogenase （LDH）. Furthermore, simulatedischemia/reperfusion （SI/R） injury in vitro was established by oxygen and glucose deprivation （OGD） for 2 h followed by 24-hour recovery period in cardiomyocytes. The activity of LDH in the cultured su- pernatant and the levels of intracellular reactive oxygen species （ROS）, SOD and MDA in cardiomyo- cytes were also measured. Finally, cardiomyocytes apoptosis was determined with flow cytometry. RESULTS： MLB significantly limited infarct size, ameliorated histopathological damages and prevented leakage of CK-MB, cTnl and LDH. Additional- ly, SOD, CAT, GPx and GSH activities were notably increased by MLB, along with the MDA content decreased as compared with the model group in rats. In vitro study, MLB also decreased LDH activity in the cultured supernatant, increased SOD activity in cardiomyocytes, reduced intracellular ROS and MDA levels, and significantly suppressed cardiomyocytes apoptosis. CONCLUSION： MLB possessed remarkably cardioprotective effects on MI/R injury in vivo and in vitro. The protection of MLB may contribute to its antioxidant properties.

关键词： Ischemia Reperfusion injury Myocytes, Cardiac Oxidative stress Antioxidants In vitro Magnesium lithospermate B

Cholinergic dysfunction-induced insufficient activation of alpha7 nicotinic acetylcholine receptor drives the development of rheumatoid arthritis through promoting protein citrullination via the SP3/PAD4 pathway

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Acta Pharmaceutica Sinica B 2023年第4期13卷 1600-1615页

作者： Changjun Lv Minghui Sun Yilei Guo Wenxin Xia Simiao Qiao Yu Tao Yulai Fang Qin Zhang yanrong zhu Yusufu Yalikun Yufeng Xia Zhifeng Wei Yue Dai Department of Pharmacology of Chinese Materia Medica School of Traditional Chinese PharmacyChina Pharmaceutical UniversityNanjing 211198China Division of Sports Medicine and Adult Reconstructive Surgery Department of Orthopedic SurgeryNanjing Drum Tower Hospitalthe Affiliated Hospital of Nanjing University Medical SchoolNanjing 210008China

Both cholinergic dysfunction and protein citrullination are the hallmarks of rheumatoid arthritis(RA),but the relationship between the two phenomena remains *** explored whether and how cholinergic dysfunction accelerates protein citrullination and consequently drives the development of *** function and protein citrullination levels in patients with RA and collageninduced arthritis(CIA)mice were *** both neuron-macrophage coculture system and CIA mice,the effect of cholinergic dysfunction on protein citrullination and expression of peptidylarginine deiminases(PADs)was assessed by *** key transcription factors for PAD4 expression were predicted and *** dysfunction in the patients with RA and CIA mice negatively correlated with the degree of protein citrullination in synovial *** cholinergic or alpha7 nicotinic acetylcholine receptor(a7nAChR)deactivation and activation resulted in the promotion and reduction of protein citrullination in vitro and in vivo,***,the activation deficiency of a7nAChR induced the earlier onset and aggravation of ***,deactivation of a7nAChR increased the expression of PAD4 and specificity protein-3(SP3)in vitro and in *** results suggest that cholinergic dysfunction-induced deficient a7nAChR activation,which induces the expression of SP3 and its downstream molecule PAD4,accelerating protein citrullination and the development of RA.

关键词： Rheumatoid arthritis Citrullination Cholinergic dysfunction a7nAChR Peptidylarginine deiminase 4 Specificity protein-3 Collagen-induced arthritis Neuron-macrophage coculture system

Tumor initiation and early tumorigenesis:molecular mechanisms and interventional targets

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Signal Transduction and Targeted Therapy 2024年第7期9卷 2900-2935页

作者： Shaosen Zhang Xinyi Xiao Yonglin Yi Xinyu Wang Lingxuan zhu yanrong Shen Dongxin Lin Chen Wu Department of Etiology and Carcinogenesis National Cancer Center/National Clinical Research Center/Cancer HospitalChinese Academy of Medical Sciences and Peking Union Medical College100021 BeijingChina Key Laboratory of Cancer Genomic Biology Chinese Academy of Medical Sciences and Peking Union Medical College100021 BeijingChina Changping Laboratory 100021 BeijingChina Collaborative Innovation Center for Cancer Personalized Medicine Nanjing Medical UniversityNanjing 211166China Sun Yat-sen University Cancer Center State Key Laboratory of Oncology in South ChinaGuangzhou 510060China CAMS Oxford Institute Chinese Academy of Medical Sciences100006 BeijingChina

Tumorigenesis is a multistep process,with oncogenic mutations in a normal cell conferring clonal advantage as the initial ***,despite pervasive somatic mutations and clonal expansion in normal tissues,their transformation into cancer remains a rare event,indicating the presence of additional driver events for progression to an irreversible,highly heterogeneous,and invasive ***,researchers are emphasizing the mechanisms of environmental tumor risk factors and epigenetic alterations that are profoundly influencing early clonal expansion and malignant evolution,independently of inducing ***,clonal evolution in tumorigenesis reflects a multifaceted interplay between cell-intrinsic identities and various cell-extrinsic factors that exert selective pressures to either restrain uncontrolled proliferation or allow specific clones to progress into ***,the mechanisms by which driver events induce both intrinsic cellular competency and remodel environmental stress to facilitate malignant transformation are not fully *** this review,we summarize the genetic,epigenetic,and external driver events,and their effects on the co-evolution of the transformed cells and their ecosystem during tumor initiation and early malignant evolution.A deeper understanding of the earliest molecular events holds promise for translational applications,predicting individuals at high-risk of tumor and developing strategies to intercept malignant transformation.

关键词： alterations mechanisms restrain

Determination of ifenprodil by LC–MS/MS and its application to a pharmacokinetic study in healthy Chinese volunteers

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Acta Pharmaceutica Sinica B 2013年第3期3卷 180-184页

作者： Jing Yang Chengtao Lu Wei Song Jiankang Li Yi Ding yanrong zhu Jinyi Cao Likun Ding Yanyan Jia Aidong Wen Department of Pharmacy Xijing HospitalFourth Military Medical UniversityShanxi 710032China

This paper reports the development and validation of an assay for ifenprodil based on liquid chromatography–tandem mass spectrometry(LC–MS/MS)and its application to a pharmacokinetic study involving single and multiple intravenous infusions to healthy Chinese *** sample preparation of plasma by liquid–liquid extraction with ethyl acetate,the analyte and internal standard,urapidil,were separated by reversed phase chromatography in a run time of 4 min and detected by positive ion electrospray ionization followed by multiple reaction monitoring of the precursor-to-product ion transitions at m/z 326.2-308.1 for ifenprodil and m/z 388.4-205.3 for *** assay was linear in the concentration range 0.2–50.0 ng/mL with recovery 476.4%.In the pharmacokinetic study of single intravenous infusions of 5,10 and 15 mg ifenprodil,peak plasma concentrations and areas under the plasma concentration–time curve were both linearly related to *** the pharmacokinetic study of multiple once daily intravenous infusions of 10 mg ifenprodil for 7 days,pharmacokinetic parameters were similar to those after the single dose showing that ifenprodil does not accumulate on repeated administration.

关键词： Ifenprodil Liquid/liquid extraction LC–MS/MS Pharmacokinetics Intravenous infusion Human Plasma

An axenic strain reveals the responses of Phaeodactylum tricornutum to external organic carbon

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Journal of Oceanology and Limnology 2024年第5期42卷 1621-1633页

作者： Zhengfeng zhu Zhichao HE Jian LI Chengxu ZHOU yanrong LI Lin ZHANG Xiaohui LI Spiros NAGATHOS Jichang HAN College of Food and Pharmaceutical Sciences Ningbo UniversityNingbo 315832China School of Biological and Chemical Engineering Panzhihua UniversityPanzhihua 617000China Ningbo Institute of Oceanography Ningbo 315832China School of Marine Life Ningbo UniversityNingbo 315832China Earth&Life Institute Catholic University of LouvainLouvain-La-Neuve 1348Belgium

The model diatom Phaeodactylum tricornutum is considered a promising source of various high value bioproducts,and developing cultivation processes is crucial for its *** mixotrophy and heterotrophy have been recommended as effective strategies for microalgal cultivation,previous studies on *** have yielded conflicting results in terms of cultivating this *** verify the capacity of this microalga utilizing external organic carbon,both heterotrophic and mixotrophic cultivation with varied carbon sources were performed using an axenic *** results demonstrate that glycerol was the only organic carbon that substantially stimulated the growth of *** in the presence of *** acetate(NaAc)at low concentrations could also promote growth,while at high concentrations led to severe inhibition under mixotrophic *** addition of glucose imposed no appreciable impact on either cell density or biomass concentration,confirming that *** cannot metabolize external ***,a comparative analysis between mixotrophy and autotrophy was performed to reveal the influences of glycerol on the cellular metabolism based on growth performances,biochemical compositions,and chlorophyll fluorescence *** also indicate that the addition of glycerol did not have detrimental effects on the capacity of either pigments biosynthesis or photosynthesis,but enhanced the saturated fatty acids and reduced the unsaturated fatty acids.

关键词： Phaeodactylum tricornutum autotrophy mixotrophy fucoxanthin glycerol

Electrochemical converting ethanol to hydrogen and acetic acid for large scale green hydrogen production

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Nano Research 2024年第3期17卷 1542-1551页

作者： Yufeng Zhang Wei zhu Jinjie Fang Zhiyuan Xu yanrong Xue Jiajing Pei Rui Sui Xingdong Wang Xuejiang Zhang Zhongbin zhuang State Key Lab of Organic-Inorganic Composites and Beijing Advanced Innovation Center for Soft Matter Science and Engineering Beijing University of Chemical TechnologyBeijing 100029China Laboratory of Advanced Functional Materials of Nanjing Nanjing Xiaozhuang UniversityNanjing 211171China Beijing Key Laboratory of Energy Environmental Catalysis Beijing University of Chemical TechnologyBeijing 100029China

Electrochemical coupling hydrogen evolution with biomass reforming reaction(named electrochemical hydrogen and chemical cogeneration(EHCC)),which realizes green hydrogen production and chemical upgrading simultaneously,is a promising method to build a carbon-neutral ***,we analyze the EHCC process by considering the market *** ethanol to acetic acid and hydrogen approach is the most feasible for large-scale hydrogen *** develop AuCu nanocatalysts,which can selectively oxidize ethanol to acetic acid(>97%)with high long-term *** isotopic and in-situ infrared experiments reveal that the promoted water dissociation step by alloying contributes to the enhanced activity of the partial oxidation reaction path.A flow-cell electrolyzer equipped with the AuCu anodic catalyst achieves the steady production of hydrogen and acetic acid simultaneously in both high selectivity(>90%),demonstrating the potential scalable application for green hydrogen production with low energy consumption and high profitability.

关键词： selective ethanol electrooxidation gold alloy catalyst electrochemical-coupled hydrogen production market assessment interfacial water activation

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动力锂离子电池正极材料的研究评述

稀有金属材料与工程 2009年第9期38卷 1687-1692页

作者：伊廷锋岳彩波朱彦荣诸荣孙胡信国安徽工业大学安徽马鞍山243002 哈尔滨工业大学黑龙江哈尔滨150001

通过衡量锂离子电池正极材料的安全性,认为LiMn2O4和LiMPO4可以作为动力电池的正极材料,综述LiMn2O4和LiMPO4正极材料的研究现状,重点对各种材料的合成、结构和性能进行总结和探讨。从目前来看,LiMn2O4仍然是主流的动力电池正极材料,但... 详细信息

通过衡量锂离子电池正极材料的安全性,认为LiMn2O4和LiMPO4可以作为动力电池的正极材料,综述LiMn2O4和LiMPO4正极材料的研究现状,重点对各种材料的合成、结构和性能进行总结和探讨。从目前来看,LiMn2O4仍然是主流的动力电池正极材料,但从长远来看,LiMPO4特别是Li3V2(PO4)3是动力锂电池正极材料的发展趋势。

关键词：动力锂离子电池正极材料 LiMn2O4 LiFePO4 Li3V2(PO4)3

Therapeutic Time Window and Underlying Therapeutic Mechanism of Breviscapinun Injection against Cerebral Ischemia/Reperfusion Injury in Rats

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Therapeutic Time Window and Underlying Therapeutic Mechanism...

Li_4Ti_5O_(12)纳米片负极材料的合成及其快速充放电性能

第十五届中国科协年会第21分会场：中药与天然药物现代研究学术研讨会

作者： GUO Chao zhu yanrong QUAN Wei YIN Ying Xi Miaomiao ZHOU Dan WEN Aidong Fourth Military Medical University

Our study was designed to investigate the therapeutic time window and underlying therapeutic mechanism of breviscapinun injection against cerebral ischemic / reperfusion injury. In the present study, Sprague-Dawley rats were subjected to middle cerebral artery occlusion for 2 h. Rats were intravenous injected with 40 mg/kg breviscapinun injection at different time-points and the same doses were administered every 24 h for 3 consecutive days. After 72 h of reperfusion, neurologic scores, infarct volume and the levels of neuron-specific enolase were measured in a masked fashion. To explore the role of HIF-1α in the neuroprotective effects of breviscapinun injection, immunohistochemistry and western blot analysis in a separate study were performed after 3 and 24 h of reperfusion. Treatment with breviscapinun significantly ameliorated neurologic deficit, reduced infarct volume and suppressed the NSE levels in a time-dependent manner. Moreover, treatment with breviscapinun increased the expression of HIF-1α protein at 3 and 24 h after reperfusion. These results suggest that the therapeutic time window of breviscapinun injection for cerebral ischemia / reperfusion injury seem to be within 5 h after reperfusion, and by increasing the expression of HIF-1α may be involved in the therapeutic mechanism of breviscapinun.

关键词： breviscapinun ischemia/reperfusion therapeutic time window hypoxia-inducible factor-1

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有色金属工程 2017年第2期7卷 6-9页

作者：梅洁朱宇朱彦荣诸荣孙伊廷锋安徽工业大学化学与化工学院安徽马鞍山243002

采用水热法合成Li_4Ti_5O_(12)负极材料,研究材料在大倍率条件下的电化学性能。X射线衍射(XRD)分析结果表明所合成的Li_4Ti_5O_(12)材料晶体尺寸在纳米级。透射电子显微镜(TEM)分析结果表明材料的结晶粒度为50~100 nm。电化学充放电测... 详细信息

采用水热法合成Li_4Ti_5O_(12)负极材料,研究材料在大倍率条件下的电化学性能。X射线衍射(XRD)分析结果表明所合成的Li_4Ti_5O_(12)材料晶体尺寸在纳米级。透射电子显微镜(TEM)分析结果表明材料的结晶粒度为50~100 nm。电化学充放电测试结果表明该材料在10 C倍率充放电时首次放电比容量达到269.9 m A·h/g,循环50次后稳定在177 m A·h/g左右,显示出优异的快速充放电性能。

关键词：锂离子电池负极材料水热合成快速充放电性能 Li4Ti5O12