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Assessment of CH_(4) flux and its influencing drivers in the rice-wheat agroecosystem of the Huai River Basin,China

Journal of Integrative Agriculture 2024年第12期23卷 4203-4215页

作者： Xiaolan Yu fangmin Zhang yanqiu fang Xiaohan Zhao Kaidi Zhang Yanyu Lu Collaborative Innovation Center on Forecast and Evaluation of Meteorological Disasters/Jiangsu Key Laboratory of Agricultural Meteorology College of Ecology and Applied MeteorologyNanjing University of Information Science&TechnologyNanjing 210044China Anhui Institute of Meteorological Sciences/Anhui Province Key Laboratory of Atmospheric Science and Satellite Remote Sensing Hefei 230031China Shouxian National Climatology Observatory Huai River Basin Typical Farm Eco-Meteorological Experiment Field of China Meteorological AdministrationShouxian 232200China

To understand the CH_(4) flux variations and their climatic drivers in the rice-wheat agroecosystem in the Huai River Basin of China,the CH_(4) flux was observed by using open-path eddy covariance at a typical rice-wheat rotation system in Anhui Province,China from November 2019 to October *** variations and their drivers were then analyzed with the Akaike information criterion ***_(4) flux showed distinct diurnal variations with single peaks during 9:00-13:00 local *** highest peak was 2.15μg m^(-2)s^(-1)which occurred at 11:00 in the vegetative growth stage in the rice growing season(RGS).CH_(4) flux also showed significant seasonal *** average CH_(4)flux in the vegetative growth stage in the RGS(193.8±74.2 mg m^(-2)d^(-1))was the highest among all growth *** annual total CH_(4) flux in the non-rice growing season(3.2 g m^(-2))was relatively small compared to that in the RGS(23.9 g m^(-2)).CH_(4) flux increased significantly with increase in air temperature,soil temperature,and soil water content in both the RGS and the non-RGS,while it decreased significantly with increase in vapor pressure deficit in the *** study provided a comprehensive understanding of the CH_(4) flux and its drivers in the rice-wheat rotation agroecosystem in the Huai River Basin of *** addition,our findings will be helpful for the validation and adjustment of the CH_(4) models in this region.

关键词： CH_(4) flux eddy covariance method rice-wheat rotation agroecosystem Huai River Basin

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Anti-oxidative effect of zinc in human umbilical cord mesenchymal stem cells

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Biophysics Reports 2021年第2期7卷 142-151页

作者： Xiaodan Lu Yifan Lin Xiuying Lin Qiang Zhang Zihang Wang Xuguang Mi Ruobing Wang Xiaofang Zhang Xu Luan Yan Liu Bing Li Yan Tan yanqiu fang Diagnostic Medical Center Jilin Province People's Hospital School of Medicine Changchun University of Chinese Medicine Department of Clinical Laboratory the Second Hospital of Jilin University General Surgery the First Hospital of Jilin University

Human umbilical cord mesenchymal stem cells(HUC-MSCs) are pluripotent and functional in many biological processes, by which releasing secretary factors to promote the self-repairing of damaged tissue or developing into functional cell at local organ. However, there is a high risk that oxidative stress would reduce the pluripotency and factor-secretion during the preparation and transplantation. Therefore, reducing oxidative stress is expected to improve the efficacy of HUC-MSCs therapy. Zinc(Zn) is an essential trace element which involves in the resistance of oxidative stress. To investigate Zn-regulated signaling pathways, we have profiled the gene expression at transcriptome level in primary HUC-MSCs treated with zinc sulfate, followed with GO and KEGG gene enrichment analysis. Zn treatment improved signal pathways for mineral absorption, cell growth, and cell death. Zn deficiency was mimicked by TPEN administration, which suppressed cell proliferation and reduced the expression of HUCMSCs surface stem cell markers CD73, CD90 and CD105 by flow cytometry. Nuclear factor erythrocyte2 related factor 2(Nrf2) plays an important role in antioxidant biological processes. In vitro treatment of Zn significantly increased Nrf2 and Sirt3 expression at gene level and protein level respectively. Zn supplementation inhibited TPEN-induced failure of cell survival and reversed the reduction of Nrf2 and Sirt3 expression, which further reduced the production of ROS. Zn successfully presented its antioxidation effect by activating Nrf2/Sirt3 signaling pathway in HUC-MSCs. Zn supplementation may improve the efficacy of HUC-MSCs therapy with reduced oxidative stress.

关键词： Zinc Oxidative stress Human umbilical cord mesenchymal stem cells Nuclear factor erythroid-2-related factor 2 Sirtuin 3

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Vascular endothelial growth factor A regulates blood cell development through EHMT

Vascular endothelial growth factor A regulates blood cell de...

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第十一届全国免疫学学术大会

作者： Lu Xiaodan fang yanqiu Zheng Yaowu Tan Yan Jilin Province People’s Hospital

The zinc-finger transcription factor GATA-3 is having important functions in T cells. It maintains hematopoietic stem cell renewal in bone marrow, leads T cell maturation in thymus, regulates Th2 cell differentiation in class II immune organs and transcripts IL-4、IL-5、IL-13 expression. EHMT1(Euchromatichistone-lysine N-methyltransferase 1) has enzymatic activity on the zinc finger domain of PRDM16 gene, directly. GATAs responses to VEGF-A expression levels. We have previously found that VEGF-A repression induced brown adipocytes differentiation in the white adipose tissue with overexpression of GATA-1 and brown adipocyte specific factor PRDM16.A tetracycline-regulated reversible VEGF-A repression transgenic mouse model(VEGFtet O/tet O:b AKtg/tg) was developed in the study. H&E staining and blood smear followed Wright’s staining were carried out for the hematological analysis. Quantitative RT-PCR, western blotting, immunofluorescence were used for measuring gene or protein expression levels in the experiments. First, we have found that the circulation red blood cell number was reduced for 20%(P<0.01) and hemoglobin was reduced for 13%(P<0.01), and lymphocyte number was increased for 28%(P<0.05). Megakaryocyte number was increased in both bone marrow and the spleen. GATA-1 and GATA-3 gene expression was increased in both bone marrow and circulation. VEGF-A repression induced GATA-1, GATA-3, PU.1, Runx1, ALAS-2, Elf-1 gene expression increase whenGATA-2 was decreased. The IL-7R+ subgroup of hematopoietic stem cell was increased in the bone marrow. EHMT1 can be regulated by VEGF-A in our result, which may directly regulate GATA-1 and GATA-3 expression by the methylation activity. The study have brought new insight of T cell development regulated by VEGF-A, which provided new evidence for the clinical use of VEGF-A or VEGF-A receptor inhibitors and T cell development related autoimmune diseases.

关键词： Vascular endothelial growth factor A EHMT1 GATA-3 Blood cell differentiation

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Mitochondrial integrity in beige adipose tissue

Mitochondrial integrity in beige adipose tissue

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第三届中国生物物理学会代谢生物学分会学术研讨会

作者：芦小单 Qiang Zhang Yinong Liu yanqiu fang Yan Tan Diagnostic Medical Center Jilin Province People’s Hospital

＜正＞The balance between energy-storage and energyexpenditure is always a systematic regulation *** normal conditions are restored,the enriched mitochondria in beige cells *** withdrawal of cold orβ3-AR agonist stimuli,there always follows mitochondria degradation and the beige-to-white transition in *** mitochondria uncoupling protein UCP1 and the oxidative phosphorylation complex OXPHOS are specific markers of mitochondria formation

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Effects of increased human tumor necrosis factor-like molecule 1A expression in peripheral blood of children with acute Guillain-Barre syndrome on interferon-gamma secretion

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Neural Regeneration Research 2009年第10期4卷 763-768页

作者： Libin Yang Shulei Li Yan Tan Shufen Xu Xiumei Duan yanqiu fang Lihua Liu Yuanyuan Che Lei Liu Liwei Zhou Department of Pediatrics First Hospital Jilin University Changchun 130021 Jilin Province China Department of Histology and Embryology Bethune Medical School Jilin University Changchun 130021 Jilin Province China Central Laboratory First Hospital Jilin University Changchun 130021 Jilin Province China

BACKGROUND： Human tumor necrosis factor-like molecule 1A （hTL1A） is a strong T helper cell type 1 （Thl） co-stimulator. Guillain-Barre syndrome （GBS） is an autoimmune disorder of the nervous system, which is mediated by Thl cells. OBJECTIVE： To determine hTL1A expression in peripheral blood T lymphocytes of acute GBS children and the effects of hTL1A on secretion of interferon-γ. DESIGN, TIME AND SETTING： A randomized, controlled, neuroimmunological in vitro study was performed at the Central Laboratory of First Hospital of Jilin University, China from November 2005 to November 2007. MATERIALS： Venous blood samples were obtained from 6 healthy donors, aged 6-12 years （all routine blood examination items were normal）, and 6 additional children with acute GBS, aged 6-12 years. The GBS children fell ill within 1 week and were not treated with hormones or immunoglobulin Purified recombinant human soluble tumor necrosis factor-like molecule 1A （rhsTL1A, 1 mg/mL, relative molecular mass 22 000, 6× His tag, soluble form） was supplied by the Central Laboratory of First Hospital of Jilin University, China. METHODS： Peripheral blood mononuclear cells were isolated from healthy donors using the standard Ficoll gradient centrifugation and were incubated in 96-well culture plates. The cells were assigned to the following groups： control （2 μg/mL phytohemagglutinin）, 2μg/mL phytohemagglutinin ＋ 25, 100 and 400 ng/mL rhsTL1A. T cell proliferation was quantified using the tritiated thymidine （3H-TdR） method. Serum interferon-γ levels in acute GBS children were detected by enzyme-linked immunosorbent assay （ELISA）. The ratio of hTL1A-positive T cells to CD3-positive T cells in peripheral blood of acute GBS children was determined using flow cytometry. Following in vitro pre-activation of peripheral blood mononuclear cells by 2 μg/mL phytohemagglutinin, the peripheral blood mononuclear cells were treated with 400 ng/mL exogenous rhsTLIA. Finally, peripheral blood mononuclear cell-secr

关键词： human tumor necrosis factor-like molecule 1 A cell proliferation Guillain-Barre syndrome interferon-γ

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IL—2在小鼠抗弓形虫作用的研究

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中国人兽共患病杂志 1998年第4期14卷 11-14页

作者：方艳秋谭岩白求恩医科大学第一临床医学院中心研究室长春130021

目的分析IL—2的抗弓形虫作用，并探讨其机制。方法采用3H一尿嘧淀（3H—U）特异性标记弓形虫在小鼠腹腔巨噬细胞（MouseperitonealmacrophageMPM）内的增殖实验及125I-UdR标记的细胞毒实验，观察IL—2在小鼠抗弓形虫中的作用。结果体... 详细信息

目的分析IL—2的抗弓形虫作用，并探讨其机制。方法采用3H一尿嘧淀（3H—U）特异性标记弓形虫在小鼠腹腔巨噬细胞（MouseperitonealmacrophageMPM）内的增殖实验及125I-UdR标记的细胞毒实验，观察IL—2在小鼠抗弓形虫中的作用。结果体外应用IL—2对MPM抗弓形虫作用无影响，体内应用IL—2能明显延长急性弓形虫感染小鼠的生存时间（P＜0.05）。IL—2治疗组小鼠脾细胞杀伤肿瘤细胞及弓形虫感染自身靶细胞的能力，明显高于对照组（P＜O.001）;正常小鼠NK细胞及LAK细胞杀伤自身感染弓形虫靶细胞的能力，显著低于杀伤肿瘤细胞的能力（P＜0.001）;IL—2诱导的LAK细胞杀伤自身感染弓形上靶细胞的能力，明显高于NK细胞（P＜0.001），且与LAK细胞杀伤肿瘤靶细胞的能力无明显差别。结论以上结果表明，IL—2体内应用提高弓形虫感染小鼠生存能力的机制，可能与提高体内LAK细胞杀伤自身感染弓形虫靶细胞的能力有关;NK细胞的抗弓形虫机制，可能在于激活NK细胞产生IFNγ和TNFα等细胞因子，间接作用于巨噬细胞而发挥作用。

关键词：弓形体 NK细胞 LAK细胞白细胞介素2 小鼠

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IL-4、TNF-α及IFN-γ诱导的小鼠腹腔巨噬细胞抗弓形虫中的作用

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中国人兽共患病杂志 1998年第6期14卷 8-10页

作者：谭岩方艳秋白求恩医科大学第一临床医学院中心实验室长春130021

目的观察IL—4、TNF—α及IFN-γ诱导的MPM在抗弓形虫中的作用。方法采用3H-尿嘧啶（3H-U）特异性标记的弓形虫在小鼠腹腔巨细胞（Mouseperitonealmacrophage，MPM）内的增殖实验。结果与结论单独应用IL—4可部分抑制弓形虫在MPM内的增... 详细信息

目的观察IL—4、TNF—α及IFN-γ诱导的MPM在抗弓形虫中的作用。方法采用3H-尿嘧啶（3H-U）特异性标记的弓形虫在小鼠腹腔巨细胞（Mouseperitonealmacrophage，MPM）内的增殖实验。结果与结论单独应用IL—4可部分抑制弓形虫在MPM内的增殖，且IL-4与IFN-γ在诱导MPM抗弓形虫的效应中具有相加作用，杭TNF-α中和抗体对IL—4诱导的弓形虫细胞内增殖抑制作用无影响;TNF—α与IFN-γ在诱导MPM抗弓形虫效应中存在协同作用，且其协同作用随TNF-α剂量的增高而明显增强。

关键词：巨噬细胞 IFNγ TNFα IL-4 弓形体病

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Effectiveness and safety of cytokine-induced killer cells cocultured with dendritic cells treatment in colorectal cancer

Effectiveness and safety of cytokine-induced killer cells co...

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第十届全国免疫学学术大会

作者： yanqiu fang Haifeng Wei Zhiqiang Ni Junjie Hou Duo Liu Yuyang Zhang Xianzhuo Jiang Yan Tan 吉林省人民医院

Objective:To evaluate efficacy and safety of dendritic cells in combination with cytokine-induced killer cell treatment of patients with colorectal ***:113 colorectal patients were enrolled in this study.61 patients accepted DC-CIK cells treatment on the basis of chemotherapy were defined as‘CIK group’and the other 52 patients accepted chemotherapy alone were defined as‘Con-trol group’.The short-term clinical curative effects and survival rate were compared between two *** host cellular immune function,quality of life(QOL)and hematological markers including serum levels of tumor markers,ratio of neutrophils to lymphocytes(N/L ratio)and levels of serum adenosine deaminase(ADA)before and after therapy were comparatively ***:The disease control rate(DCR)was significantly higher in CIK group than in the control group(P=0.045)and more biotherapies(>2 cycle)could benefit patients in higher DCR compared with fewer biotherapies(1 cycle)(P=0.022).No statistically significant difference was found in the overall survival between the two groups(P=0.254),whereas compared to patients in the control group,the patients in CIK group had significantly longer progression-free survival(P=0.018).The ratio of neutrophils to lymphocytes(N/L ratio)was significantly lower in CIK group than in the control group(2.74±1.31 vs.2.32±0.98,P=0.046).The serum levels of tumor markers,including CA19-9,CA24-2 and CEA,were significantly decreased after DC-CIK cells *** host immune function was enhanced and QOL was improved in the patients treated by DC-CIK biotherapy compared to the patients treated by chemotherapy *** most common side effects were fever(15.8%),chill(8.8%),headache(5.3%),nausea and vomiting(5.3%)and skin rash(1.8%).No severe side effects occurred in the DC-CIK cells transfusion patients and no toxic reaction resulted from DCCIK cells ***:DC-CIK immunotherapy is a potential effective and safe approach for patients with colo

关键词： Colorectal cancer Dendritic cells Cytokine-Induced Killer cells Immunotherapy Clinical research

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大麻二酚的经皮促渗方法及促渗机制研究

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中国医药工业杂志 2022年第4期53卷 507-514页

作者：陈博琪张定王曙宾方燕秋汪晴大连理工大学化工学院药学系精细化工国家重点实验室辽宁大连116024 汉义生物科技(北京)有限公司北京100020 广东红珊瑚药业有限公司广东东莞523808

本研究分别考察了化学促渗剂与表面活性剂泊洛沙姆101(P101)经预混法和皮肤预处理法给药对大麻二酚(CBD)在离体裸鼠皮肤上经皮渗透的影响,进一步通过衰减全反射傅里叶变换红外光谱、分子动力学模拟及分子对接等方法,对相关促渗机制进行... 详细信息

本研究分别考察了化学促渗剂与表面活性剂泊洛沙姆101(P101)经预混法和皮肤预处理法给药对大麻二酚(CBD)在离体裸鼠皮肤上经皮渗透的影响,进一步通过衰减全反射傅里叶变换红外光谱、分子动力学模拟及分子对接等方法,对相关促渗机制进行了分析。结果表明,相较于预混法,皮肤预处理方法显示出较佳的促渗效果,其中采用1% P101预处理皮肤促进CBD渗透的效果最强,相应促渗系数达到12.97。分子动力学模拟结果显示,P101与神经酰胺NS(皮肤角质层中的脂质分子)相互作用较强,对接能为–8.85 kcal/mol;P101可使脂质双分子层体系的内聚能密度由4.15×10^(8)kcal/mol降至3.87×10^(8)kcal/mol,说明P101的促渗作用是由于其扰乱了皮肤角质层脂质双分子层的有序结构,增加了流动性。该研究对研发CBD经皮给药方案具有重要的指导意义。

关键词：大麻二酚经皮渗透促渗机制泊洛沙姆分子动力学模拟分子对接

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K-ras抗原致敏的DC诱导CTL对胰腺癌体内杀伤活性的研究

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中国肿瘤临床 2010年第8期37卷 421-425页

作者：谭广徐立方艳秋陶钢王忠裕谭岩大连医科大学附属第一医院普外二科辽宁省大连市116011 吉林大学第二医院检验科吉林大学第一医院中心实验室解放军第210医院普外科

目的:研究K-ras多肤的致敏树突状细胞(DC)活化的特异性细胞毒性T淋巴细胞(CTL)对胰腺癌的体内外杀伤作用。方法:联合应用粒细胞-巨噬细胞集落刺激因子和白细胞介素-4诱导培养外周血DC。表达K-ras突变体的胰腺癌细胞株全瘤、单纯K-ras突... 详细信息

目的:研究K-ras多肤的致敏树突状细胞(DC)活化的特异性细胞毒性T淋巴细胞(CTL)对胰腺癌的体内外杀伤作用。方法:联合应用粒细胞-巨噬细胞集落刺激因子和白细胞介素-4诱导培养外周血DC。表达K-ras突变体的胰腺癌细胞株全瘤、单纯K-ras突变体多肽和K-ras突变体表位肽阳离子纳米颗粒分别致敏DC。致敏DC刺激T淋巴细胞得到肿瘤抗原特异的细胞毒性T淋巴细胞(CTL)。Patu8988、SW1990细胞系制备荷瘤裸鼠模型评价CTL体内抗肿瘤活性。结果:负载全瘤抗原的DC其诱导产生的CTL对胰腺癌有较好的抑制,负载单纯K-ras(12-Val)突变体多肽、K-ras(12-Val)突变体表位肽阳离子纳米颗粒的DC其诱导产生的CTL对表达K-ras(12-Val)突变体阳性(Patu8988)的胰腺癌有较特异的抑制作用,而对K-ras(12-Val)突变体阴性(SW1990)的胰腺癌的抑制作用与对照组比较无显著性差异结论:负载肿瘤抗原的DC诱导的CTL可显著提高对荷瘤裸鼠的生存时间,抑制肿瘤的生长速度,并显示其可增加抗肿瘤特异性。

关键词：胰腺癌树突状细胞 K-ras多肽细胞毒T淋巴细胞

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