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Effects of cell cycle on telomerase activity and on hepatitis B virus replication in HepG₂2.2.15 cells

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Hepatobiliary & Pancreatic Diseases International 2004年第4期3卷 543-547页

作者： yanqing huang LuWen Wang ShaoNan Yan ZuoJiong Gong Department of Infectious Diseases Renmin Hospital Wuhan University Wuhan 430060 China.

BACKGROUND:It has been shown that telomerase activity and hepatitis B virus (HBV) replication are closely associated with cell cycle. This study aimed to further investigate the effects of cell cycle on telomerase activity and on HBV replication.;METHODS:Human hepatoma cells transfected with HBV DNA (HepG2 2.2.15 cell line) were treated respectively with serum deprivation, all-trans retinoic acid (RA), dimethyl sulfoxide (DMSO), or sodium butyrate. The cell cycle of HepG2 2.2.15 cells was analyzed by flow cytometry. The telomerase activities of the cells were detected by TRAP-PCR-ELISA. HBV DNA in culture medium was assayed by a fluorescent quantitative PCR assay and a semiquantitative dot blot hybridization technique. HBsAg and HBeAg in culture media were quantitatively examined by an ELISA assay.;RESULTS:Treatments with serum deprivation, RA, DMSO, or sodium butyrate inhibited the proliferation of HepG2 2.2.15 cells and led to cell arrest in the G0/G1 phase of cell cycle. The percentage of the G0/G1 phase in the groups of sodium butyrate, DMSO, RA and serum-free was 85.2%, 71.9%, 68.3% and 65.2%, respectively, but in the control group, 43.1% (P<0.01). The activities of telomerase of the cells were also significantly inhibited by 82.8%, 74.6%, 76.1% and 69.4% respectively. In addition, HBV replication of the HepG2 2.2.15 cells remarkably increased as shown by the contents of HBV DNA, HBsAg and HBeAg in the culture media of the cells treated with sodium butyrate, DMSO, RA or serum deprivation (P<0.01). The amounts of HBV DNA in the groups of sodium butyrate, DMSO, RA, serum deprivation and control were 6.7X10(6), 4.8X10(6), 4.4X10(6), 5.1X10(6) and 1.2X10(6) copies/ml, respectively (P<0.01). Telomerase was expressed mainly in the cells in S phase. HBV replication increased in quiescent cells (G0/G1 phase), and decreased in proliferating phase (S phase).;CONCLUSIONS:The current data approve that HBV replication is associated with the cellular proliferative activity.

关键词： cell cycle telomerase hepatitis B virus HepG2 2.2.15 replication

A thermal-mechanical constitutive model for b-HMX single crystal and cohesive interface under dynamic high pressure loading

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Science China(Physics,Mechanics & Astronomy) 2010年第2期53卷 218-226页

作者： WU yanqing & huang FengLei State Key Laboratory of Explosion Science and Technology,Beijing Institute of Technology,Beijing 100081,China Beijing Institute of Technology State Key Laboratory of Explosion Science and Technology Beijing China (GRID:grid.43555.32) (ISNI:***)

Due to the significant thermal-mechanical effects during hot spot formation in PBX explosives,a thermodynamic constitutive model has been constructed for HMX anisotropic single crystal subjected to dynamic impact loading. The crystal plasticity model based on dislocation dynamics theory was employed to describe the anisotropic plastic behavior along the preferential slip systems. A modified equation of state (EOS) was introduced into the constitutive equations through the decomposing stress tensor and the nonlinear elasticity for materials was taken into account. The one-dimensional strain impact simulations for HMX single crystal and quasi-bicrystal were performed respectively,in which the cohesive elements were inserted over the interface areas for the latter. The predicted particle velocities for the single crystal sample agreed well with the experimental results in the literature. Furthermore,the effects of crystal orientations,interface,misorientations on localized strain,stress and temperature distributions were predicted and discussed.

关键词： energetic single crystal dynamic high pressure crystal plasticity thermodynamics interfacial model

Hole mobility enhancement in monolayer WSe2 p-type transistors through molecular doping

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Science China(Information Sciences) 2024年第6期67卷 207-213页

作者： Shiyuan LIU Xiong XIONG Xin WANG Xinhang SHI Ru huang yanqing WU School of Integrated Circuits and Beijing Advanced Innovation Center for Integrated Circuits Peking University Wuhan National High Magnetic Field Center and School of Integrated Circuits Huazhong University of Science and Technology

Two-dimensional （2D） transition metal dichalcogenide （TMDC） semiconductor materials exhibit extraordinary electrical properties,holding promise for the realization of next-generation complementary metal-oxide-semiconductor （CMOS） devices at ultimate ***,constrained by effective device doping strategies,the hole mobility and device performance of tungsten diselenide （WSe2） p-type transistors,especially monolayer chemical vapor deposition （CVD）-grown WSe2,have not met *** this paper,an effective performance enhancement of monolayer WSe2p-type transistor was achieved through a molecular doping *** monolayer WSe2directly on SiO2back-gated substrates and leveraging energy band alignment design,4-nitrobenzenediazonium tetrafluoroborate （4-NBD） molecular dopant with a concentration of 10 m M was utilized to modulate the Fermi level position of monolayer WSe2for hole *** devices demonstrated a more than 98%increase in hole mobility,reaching up to 97 cm2·V-1·s-1while maintaining the current on/off ratio of *** p-type WSe2transistors with 1μm channel length exhibit a high drive current surpassing 176μA·μm-1,exceeding previous CVD-WSe2devices with similar channel *** straightforward and effective approach to improving the electrical performance of WSe2transistors paves the way for advanced logic technologies based on transition metal dichalcogenide semiconductors.

关键词： CVD-grown WSe$_2$ molecular doping 4-NBD p-type transistors hole mobility

Characterization of favorable lithofacies in tight sandstone reservoirs and its significance for gas exploration and exploitation: A case study of the 2nd Member of Triassic Xujiahe Formation in the Xinchang area, Sichuan Basin

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Petroleum Exploration and Development 2020年第6期47卷 1194-1205页

作者： LIU Junlong LIU Zhongqun XIAO Kaihua huang yanqing JIN Wujun Sinopec Exploration&Production Research Institute Beijing 100083China

By using core,logging curves,and experiment data,favorable lithofacies types in the 2 nd Member of Triassic Xujiahe Formation in the Xinchang area,Sichuan Basin were classified,standard of the favorable lithofacies was established,planar distribution regularities of the favorable lithofacies were identified,and forming mechanisms of the favorable lithofacies and their control effect on production were examined.(1)The 2 nd Member of Xujiahe Formation has twelve types of lithofacies,among which multiple layer medium-coarse grain sandstone lithofacies,parallel bedding medium-coarse grain sandstone lithofacies,massive bedding medium-coarse grain sandstone lithofacies,inclined bedding medium-coarse grain sandstone lithofacies,and charcoal-bearing medium-coarse grain sandstone lithofacies with better physical properties and higher gas content are favorable lithofacies;they feature low gamma,low neutron porosity,low resistivity,and high acoustic travel time on logging curves.(2)The sedimentary process controls spatial distribution of sand bodies which are the material basis of the favorable lithofacies;post diagenetic fluids would differentially reconstruct the favorable lithofacies;tectonic activities and abnormal formation pressure made strata slide along the weakness plane,giving rise to fractures in different types of rocks,which can enhance the reservoir permeability significantly.(3)The development degree of favorable lithofacies is a major factor affecting stable production of gas well.

关键词： tight sandstone reservoir favorable lithofacies reservoir characterization Triassic Xujiahe Formation Xinchang area Sichuan Basin

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Numerical study on the loss of fast ions produced by minority ion cyclotron resonance heating in EAST

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Plasma Science and Technology 2021年第11期23卷 1-8页

作者： Dingzong ZHANG Youjun HU Nong XIANG Cheng YANG Wei SHEN yanqing huang Hongbo LIU College of Physics and Electronic Engineering.Hengyang Normal University Hengyang 421008People's Republic of China Institute of Plasma Physics Chinese Academy of SciencesHefei 230031People's Republic of China

The classical prompt loss of fast ions produced by minority ion cyclotron resonance heating(ICRH)is studied by a guiding center orbit following code in the Experimental Advanced Superconducting Tokamak(EAST).It is found that the loss of fast ions produced by ICRH mainly appears in both ends of the resonance layer,while the loss of fast ions in the middle resonance layer is very *** dominant fast loss comes from trapped ions,rather than from passing *** the location of resonance layer at the plasma core may be more beneficial to the EAST tokamak *** addition,the loss distribution of fast ions is *** results show that the fast ions are mainly lost near the midplane in the poloidal direction,but almost uniformly in the toroidal ***,we investigate the dependence of fast ion loss on the ICRH *** simulation results show that the loss fraction of fast ions in both ends of the resonance region increases with the ion cyclotron range of frequencies(ICRF)power,but barely affects the loss of fast ions in the middle region.

关键词： ion cyclotron resonance heating the loss of fast ions EAST

Update in version 2021 of CSCO guidelines for colorectal cancer from version 2020

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Chinese Journal of Cancer Research 2021年第3期33卷 302-307页

作者： Caixia Dong Yuwei Ding Shanshan Weng Guichao Li yanqing huang Hanguang Hu Zhen Zhang Suzhan Zhang Ying Yuan Department of Medical Oncology The Second Affiliated Hospital of Zhejiang University School of MedicineHangzhou 310009China Cancer Center Zhejiang UniversityHangzhou 310058China Cancer Institute Key Laboratory of Cancer Prevention and InterventionMinistry of EducationThe Second Affiliated Hospital of Zhejiang University School of MedicineHangzhou 310009China Department of Radiation Oncology Fudan University Shanghai Cancer HospitalShanghai 200032China Department of Colorectal Surgery The Second Affiliated Hospital of Zhejiang University School of MedicineHangzhou 310009China

Colorectal cancer(CRC) is one of the major cancers threatening life and health of Chinese residents. According to the latest data released by the National Cancer Center in2015(1), there were 387,000 new cases and 187,100 deaths of CRC in China, accounting for 9.87% and 8.01% of all malignancies. The first version of the Chinese Society of Clinical Oncology(CSCO) guidelines was released in April2017, and has been updated annually based on the latest clinical research data and the availability of new drugs(2-4). Here, we present the main updates of the 2021 version compared to 2020 version.

关键词： CSCO cancer drugs

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基于文献计量学生地黄药理作用的研究

中医临床研究 2024年第6期16卷 1-8页

作者：郑惠文沈晓杰黄艳青王佳慧郑洋李卫民广西中医药大学赛恩斯新医药学院广西南宁530222 广西中医药大学广西南宁530222

目的:文章从文献计量学角度分析生地黄相关研究分布、来源出版物、研究机构以及引文情况,为了解生地黄相关文献的研究热点和研究方向提供参考依据。方法:采用PubMed核心合集数据库和中国知网数据库获得文献数据,运用文献计量学的方法和C... 详细信息

目的:文章从文献计量学角度分析生地黄相关研究分布、来源出版物、研究机构以及引文情况,为了解生地黄相关文献的研究热点和研究方向提供参考依据。方法:采用PubMed核心合集数据库和中国知网数据库获得文献数据,运用文献计量学的方法和CiteSpace软件对有关生地黄这一研究主题的文献、期刊以及关键词等进行可视化分析。结果:近40年在生地黄领域研究热点为生地黄拥有调节免疫功能、抗衰老、促进造血、抗肿瘤、抗氧化的药效。共搜索到有效中英文文献1 071篇,其中中国知网中收录676篇,PubMed中收录395篇;在中国知网中生地黄相关文献发文量最多的作者为谢宁(28篇),在PubMed核心合集数据库中生地黄相关文献发文量最多为WANG J(13篇);中国知网中重要发文期刊为《中国实验方剂学杂志》(46篇),PubMed中重要发文期刊为《Zhongguo Zhong Yao Za Zhi》(21篇);资助文献量最多的基金为国家自然科学基金,共资助文献244篇。结论:深入了解该领域重要作者、文献、期刊、机构等信息可以获得更多突破性的成果及思路,为今后临床上的科研工作者提供理论依据。在近40年生地黄研究过程中,研究热点随着时间在不断变化,近些年来主要热点为地黄寡糖、细胞增殖、胰岛素抵抗、化学成分、药理作用等方面。目前对于生地黄研究仍有不足,各研究作者联系不强,生地黄研究主观性较强,缺乏统一标准,作用机制尚不明确,有待进一步研究以得到深入的研究数据。

关键词：生地黄文献计量学可视化分析药理作用

The transcriptional control of LcIDL1–LcHSL2 complex by LcARF5 integrates auxin and ethylene signaling for litchi fruitlet abscission

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Journal of Integrative Plant Biology 2024年第6期66卷 1206-1226页

作者： Xingshuai Ma Zidi He Ye Yuan Zhijian Liang Hang Zhang Vilde Olsson Lalun Zhuoyi Liu yanqing Zhang Zhiqiang huang Yulian huang Jianguo Li Minglei Zhao State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources Guangdong Laboratory for Lingnan Modern AgricultureKey Laboratory of Biology and Genetic Improvement of Horticultural Crops(South China)Ministry of Agriculture and Rural AffairsGuangdong Litchi Engineering Research CenterCollege of HorticultureSouth China Agricultural UniversityGuangzhou 510642China Dongguan Botanical Garden Dongguan 523128China Section for Genetics and Evolutionary Biology Department of BiosciencesUniversity of OsloBlindernveien 31Oslo 0316Norway College of Agriculture Guangxi UniversityNanning 530004China

At the physiological level,the interplay between auxin and ethylene has long been recognized as crucial for the regulation of organ abscission in ***,the underlying molecular mechanisms remain ***,we identified transcription factors involved in indoleacetic acid(IAA)and ethylene(ET)signaling that directly regulate the expression of INFLORESCENCE DEFICIENT IN ABSCISSION(IDA)and its receptor HAESA(HAE),which are key components initiating ***,litchi IDAlike 1(LcIDL1)interacts with the receptor HAESA-like2(LcHSL2).Through in vitro and in vivo experiments,we determined that the auxin response factor LcARF5 directly binds and activates both LcIDL1 and ***,we found that the ETHYLENE INSENSITIVE 3-like transcription factor LcEIL3 directly binds and activates *** expression of IDA and HSL2 homologs was enhanced in Lc ARF5and Lc EIL3 transgenic Arabidopsis plants,but reduced in ein3 eil1 ***,the expressions of LcIDL1 and LcHSL2 were significantly decreased in Lc ARF5-and LcEIL3-silenced fruitlet abscission zones(FAZ),which correlated with a lower rate of fruitlet *** of auxin led to an increase in 1-aminocyclopropane-1-carboxylic acid(the precursor of ethylene)levels in the litchi FAZ,followed by abscission *** this process,LcRF5 and LcEIL3 were induced in the ***,our findings suggest that the molecular interactions between litchi AUXIN RESPONSE FACTOR 5(LcARF5)-LcIDL1/LcHSL2 and LcEIL3–LcIDL1 signaling modules play a role in regulating fruitlet abscission in litchi and provide a long-sought mechanistic explanation for how the interplay between auxin and ethylene is translated into the molecular events that initiate abscission.

关键词： auxin ethylene fruit abscission IDA–HAE/HSL2 litchi

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目标探测对记忆提取的影响

心理学报 2020年第6期52卷 706-715页

作者：黄晏清孟迎芳福建师范大学心理学院福州350117 三明学院教育与音乐学院福建三明365004

采用经典的"学习–再认"范式,在测验阶段设置了目标探测的干扰任务,通过比较目标探测和分心拒绝是否会对记忆提取产生不同的影响,以进一步探讨提取干扰与再认记忆之间的关系。结果发现,无论对目标进行显性(实验1)还是隐性(实验2)的探测... 详细信息

采用经典的"学习–再认"范式,在测验阶段设置了目标探测的干扰任务,通过比较目标探测和分心拒绝是否会对记忆提取产生不同的影响,以进一步探讨提取干扰与再认记忆之间的关系。结果发现,无论对目标进行显性(实验1)还是隐性(实验2)的探测反应,目标探测下旧词的再认反应均要比分心拒绝下更优,表明外显记忆的提取也会受到注意资源的调节。但更为重要的是,在辨别力指数(d′)及判断标准(C)上的差异分析发现,目标探测并没有提高被试的再认水平,而是降低了被试的判断标准,使得被试在目标探测条件下更容易对词做出"旧"反应。这种反应倾向与目标探测的动作反应方式无关,也不受词汇的编码加工水平深浅的调节。由此可见,外显记忆提取并非完全的"自动化加工",仍会受到注意资源的调节。

关键词：注意促进效应目标探测记忆提取双任务