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Exploration of reaction rates of chlorine dioxide with tryptophan residue in oligopeptides and proteins

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Journal of Environmental Sciences 2020年第7期32卷 129-136页

作者： Yuexian ge Yu Lei Xin Lei Wenhui Gan Longfei Shu Xin Yang School of Environmental Science and Engineering Guangdong Provincial Key Laboratory of Environmental Pollution Control and Remediation TechnologySun Yat-sen UniversityGuangzhou 510275China Graduate School at Shenzhen Tsinghua UniversityShenzhen 518055China

Chlorine dioxide(ClO2),an alternative disinfectant to chlorine,has a superior ability to inactivate microorganisms,in which protein damage has been considered as the main inactivation ***,the reactivity of ClO2 with amino acid residues in oligopeptides and proteins remains poorly *** this research,we studied the reaction rate constants of ClO2 with tryptophan residues in five heptapeptides and four proteins using stopped-flow or competition kinetic *** heptapeptide and protein contain only one tryptophan residue and the reactivity of tryptophan residue with ClO2 was lower than that of free tryptophan(3.88×10^4(mol/L)^-1 sec^-1 at pH 7.0).The neighboring amino acid residues affected the reaction rates through promoting inter-peptide aggregation,changing electron density,shifting pKa values or inducing electron transfer via redox reactions.A single amino acid residue difference in oligopeptides can make the reaction rate constants differ by over 60%(e.g.3.01×10^4(mol/L)^-1 sec^-1 for DDDWNDD and 1.85×10^4(mol/L)^-1 sec^-1 for DDDWDDD at pH 7.0(D:aspartic acid,W:tryptophan,N:asparagine)).The reaction rates of tryptophan-containing oligopeptides were also highly pH-dependent with higher reactivity for deprotonated tryptophan than the neutral *** residues in proteins spanned a 4-fold range reactivity toward ClO2(i.e.0.84×10^4(mol/L)^-1 sec^-1 for ribonuclease T1 and 3.21×10^4(mol/L)^-1 sec^-1 for melittin at pH 7.0)with accessibility to the oxidant as the determinating *** local environment surrounding the tryptophan residue in proteins can also accelerate the reaction rates by increasing the electron density of the indole ring of tryptophan or inhibit the reaction rates by inducing electron transfer *** results are of significance in advancing understanding of ClO2 oxidative reactions with proteins and microbial inactivation mechanisms.

关键词： Chlorine dioxide(ClO2) Tryptophan Protein Disinfection Water treatment

山药DoWRKY40基因表达特征分析及互作蛋白筛选

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生物技术通报 2024年第8期40卷 118-128页

作者：邢丽南张艳芳葛明然赵令敏陈妍霍秀文内蒙古农业大学园艺与植物保护学院内蒙古自治区野生特有蔬菜种质资源与种质创新重点实验室呼和浩特010019

【目的】WRKY作为植物特异型转录因子,参与植物生长发育过程。克隆山药DoWRKY40基因,分析其表达模式,通过酵母双杂交技术筛选与DoWRKY40互作的蛋白,探究WRKY40在山药膨大过程中的调控机制。【方法】以‘大和长芋’山药为材料克隆DoWRKY4... 详细信息

【目的】WRKY作为植物特异型转录因子,参与植物生长发育过程。克隆山药DoWRKY40基因,分析其表达模式,通过酵母双杂交技术筛选与DoWRKY40互作的蛋白,探究WRKY40在山药膨大过程中的调控机制。【方法】以‘大和长芋’山药为材料克隆DoWRKY40基因,并进行生物信息学分析、表达模式分析、亚细胞定位,构建山药块茎不同发育时期酵母文库,筛选与DoWRKY40互作蛋白。【结果】DoWRKY40的开放阅读框长为927 bp。DoWRKY40蛋白属于WRKY家族的第II组,含有一个典型的WRKY转录因子保守结构域,定位于细胞核。与几内亚薯蓣亲缘关系较近。DoWRKY40基因在山药块茎生长发育的120 d表达量最高,且在叶、茎和块茎中均有表达,具有组织特异性。山药cDNA文库的库容量为1.484×10^(8);pGBKT7-WRKY40诱饵载体无自激活活性。酵母双杂交法从山药文库中筛选到4类与DoWRKY40相互作用的蛋白,分别参与植物生长发育、细胞周期调节与细胞扩增、信号转导及环境胁迫应答等。【结论】克隆得到DoWRKY40基因,其响应山药的生长发育过程,筛选到的4类互作蛋白表明其在山药细胞膨大及信号转导中起调控作用。

关键词：山药 WRKY40基因表达特征亚细胞定位 cDNA文库酵母双杂交互作蛋白筛选

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我国竞技排球文化:内涵特征及建设路径

体育文化导刊 2024年第5期 34-40页

作者：陈华伟赵敏敏朱新妍刘昕格李牧北京体育大学中国排球运动学院北京100084 陕西理工大学体育学院陕西汉中723000 北京市体育局北京100075

运用文献资料、逻辑分析等方法,梳理我国竞技排球发展历程,分析文化内涵特征,并提出建设路径。发展历程:规范发展期、快速突破期、改革探索期、全面提升期。内涵特征:以满足项目发展需求,建设符合国际竞赛标准的配套设施为基础;以制定... 详细信息

运用文献资料、逻辑分析等方法,梳理我国竞技排球发展历程,分析文化内涵特征,并提出建设路径。发展历程:规范发展期、快速突破期、改革探索期、全面提升期。内涵特征:以满足项目发展需求,建设符合国际竞赛标准的配套设施为基础;以制定符合项目发展规律、全国上下一盘棋的制度为保障;以契合项目特点的价值观与思维方式为内核。建设路径:丰富物质文化种类,创新制度文化建设,突出精神文化内涵。

关键词：体育管理竞技排球内涵特征项目文化

Physicochemical properties of respiratory droplets and their role in COVID-19 pandemics:a critical review

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Biomaterials Translational 2021年第1期2卷 10-18页

作者： Ting ge Shengfeng Cheng Department of Chemistry and Biochemistry University of South CarolinaColumbiaSCUSA Department of Physics Department of Mechanical EngineeringCenter for Soft Matter and Biological Physicsand Macromolecules Innovation InstituteVirginia Polytechnic Institute and State UniversityBlacksburgVAUSA

The ongoing coronavirus disease 2019(COVID-19)pandemic is a serious challenge faced by the global *** scientists can help medical workers and biomedical scientists,engineers,and practitioners,who are working on the front line,to slow down and eventually contain the spread of the COVID-19 *** review is focused on the physicochemical characteristics,including composition,aerodynamics,and drying behavior of respiratory droplets as a complex and multicomponent soft matter system,which are the main carrier of the virus for interpersonal *** distribution and dynamics of virus particles within a droplet are also *** the characteristics of virus-laden respiratory droplets can lead to better design of personal protective equipment,frequently touched surfaces such as door knobs and touchscreens,and filtering equipment for indoor air *** an understanding also provides the scientific basis of public policy,including social distancing rules and public hygiene guidelines,implemented by governments around the world.

关键词： aerosol COVID-19 evaporation pandemic respiratory droplet virus

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m^(6)A修饰与植物RNA病毒侵染

中国科学：生命科学 2024年第3期54卷 447-458页

作者：葛林豪潘福安何浩贾明轩李方方周雪平中国农业科学院植物保护研究所植物病虫害综合治理全国重点实验室北京100193 浙江大学生物技术研究所水稻生物学国家重点实验室杭州310058

N6-甲基腺嘌呤(N6-methyladenosine,m^(6)A)是真核生物m RNA中丰度最高的RNA转录后化学修饰.RNA的m^(6)A修饰主要由甲基化转移酶(writers)、去甲基化酶(erasers)以及阅读蛋白(reader proteins)共同调控.近年的研究表明,m^(6)A修饰在植物病毒侵染中发挥了重要作用,相关调控机制成为植物病毒领域的研究热点.本文概述了植物RNA m^(6)A修饰相关蛋白的基本组成和m^(6)A修饰的检测技术,重点阐述了m^(6)A修饰在植物与RNA病毒互作中的作用,并提出了今后植物RNA病毒m^(6)A修饰功能研究的方向.

关键词： m^(6)A修饰植物植物RNA病毒

Morphology-controlled CoNi/C hybrids with bifunctions of efficient anti-corrosion and microwave absorption

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Journal of Materials Science & Technology 2022年第7期102卷 24-35页

作者： Jianwen ge Yu Cui Jiaxin Qian Li Liu Fandi Meng Fuhui Wang Shenyang National Laboratory for Materials Science Northeastern UniversityShenyang 110819China Shi-changxu Innovation Center for Advanced Materials Institute of Metal ResearchChinese Academy of SciencesShenyang 110016China

Till now,fewer literatures have investigated the corrosion resistance of microwave absorbers,especially mental@C/carbon matrix derived from MOF composites,although it is known that carbon shells possess a protective ***,three kinds of morphology-controlled CoNi/C-N doped architectures were suc-cessfully fabricated via a sequence of processing,namely coprecipitation for ZIF-67,subsequent Ni^(2+) ex-change and ultimate *** from composition characterization,the effects of microstructure tailoring and temperature controlling on electromagnetic response as well as attenuation performance were revealed,where dodecahedron-shaped composites possessed the highest *** contrast,rod-shaped composites(CoNi/C-r-550 and CoNi/C-r-700)were endowed with superior comprehensive ab-sorption properties,e.g.,RL_(min):-49.8 dB and-64.0 dB;EAB:5.7 GHz and 4.8 GHz,***,samples CoNi/C-d-700 and CoNi/C-r-700 present higher corrosion potential(E_(corr))and lower corrosion current(I corr).Hence,these corrosion-resistant microwave absorbers with outstanding absorption stabil-ity,wetting effect as well as environmental adaptability,can be used as a candidate/raw material for intelligent devices.

关键词： Electromagnetic response Morphological-anisotropic Anticorrosive ZIF-67

Molecular dynamics simulations of ovalbumin adsorption at squalene/water interface

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Chinese Journal of Chemical Engineering 2022年第10期50卷 369-378页

作者： Qingxia Xiong Ying Ren Yufei Xia Guanghui Ma Reiji Noda Wei ge State Key Laboratory of Multiphase Complex Systems Institute of Process EngineeringChinese Academy of SciencesBeijing 100190China Division of Environmental Engineering Science Graduate School of Science and TechnologyGunma University1-5-1 Tenjin-choKiryuGunma 376-8515Japan State Key Laboratory of Biochemical Engineering Institute of Process EngineeringChinese Academy of SciencesBeijing 100190China School of Chemical Engineering University of Chinese Academy of SciencesBeijing 100049China Innovation Academy for Green Manufacture Chinese Academy of SciencesBeijing 100190China

The adsorption of protein molecules to oil/water(O/W)interface is of critical importance for the product design in a wide range of technologies and industries such as biotechnology,food industry and pharmaceutical *** this work,with ovalbumin(OVA)as the model protein,the adsorption conformations at the O/W interface and the adsorption stability have been systematically studied via multiple simulation methods,including all-atom molecular dynamic(AAMD)simulations,coarse-grained molecular dynamic(CGMD)simulations and enhanced sampling *** computational results of AAMD and CGMD show that the hydrophobic tail of OVA tends to be folded under long time relaxation in aqueous phase,and multiple adsorption conformations can exist at the interface due to heterogeneous interactions raising from oil and water *** further study the adsorption sites of the protein,the adsorption kinetics of OVA at the O/W interface is simulated using metadynamics method combined with CGMD simulations,and the result suggests the existence of multiple adsorption conformations of OVA at interface with the head-on conformation as the most stable *** all,this work focuses on the adsorption behaviors of OVA at squalene/water interface,and provides a theoretical basis for further functionalization of the proteins in emulsion-based products and engineering.

关键词： Molecular dynamicssimulation Metadynamics Protein adsorption Structural stability Ovalbumin

Concept and requirements of sustainable development in bridge engineering

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Frontiers of Structural and Civil Engineering 2011年第4期5卷 432-450页

作者： Yaojun ge Haifan XIANG State Key Laboratory for Disaster Reduction in Civil Engineering Tongji UniversityShanghai 200092China

The concept of sustainability is described in this paper using a single sustainable principle,two goals of sustainable development,three dimensions of sustainable engineering,four sustainable requirements and five phases of sustainable *** sustainable requirements and their practice in China are discussed in *** safe reliability of bridges is first compared with the events of bridge failure in China and in the rest of the world and followed by structural durability,including the cracking of concrete cable-stayed bridges,deflection of concrete girder bridges and fatigue cracks of orthotropic steel *** respect to functional adaptability,lateral wind action on vehicles and its improvement are introduced regarding a sea-crossing bridge located in a typhoon-prone *** Chinese practice of using two double main span suspension bridges and a twin parallel deck cable-stayed bridge is presented in discussing the final sustainable requirement:capacity extensibility.

关键词： sustainable engineering safe reliability structural durability functional adaptability capacity extensibility

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根-土复合体抗剪强度影响因素研究

应用力学学报 2024年第4期41卷 804-811页

作者：陈攀葛永刚孙庆敏孙聿卿张永波中国科学院水利部成都山地灾害与环境研究所成都610041 中国科学院山地灾害与地表过程重点实验室成都610041 西藏大学工学院拉萨850000 中国科学院大学北京100049

为比较根-土复合体组分及法向应力对其抗剪强度的影响程度,选取四川省喜德县冕山镇坡面(安宁河流域砾砂区)云南松根-土复合体和素土作为研究对象,通过室内直剪试验获得其抗剪强度,烘干试验获得各组分质量,利用根-土复合体的干土质量、... 详细信息

为比较根-土复合体组分及法向应力对其抗剪强度的影响程度,选取四川省喜德县冕山镇坡面(安宁河流域砾砂区)云南松根-土复合体和素土作为研究对象,通过室内直剪试验获得其抗剪强度,烘干试验获得各组分质量,利用根-土复合体的干土质量、水质量和干根质量进行Pearson相关分析,构建回归分析模型计算根-土复合体各组分质量与抗剪强度的标准化回归系数。结果表明:根-土复合体的干土质量与干根质量间存在显著负相关关系(P=0.0020.05);云南松根-土复合体中干土质量、干根质量和水的质量与抗剪强度的标准化回归系数分别为0.06、0.03和0.03,表明干土质量对根-土复合体抗剪强度的影响程度较水的质量和干根质量的影响程度大;与素土的内摩擦角和黏聚力相比,云南松根-土复合体的内摩擦角增加了1.31%~1.97%,黏聚力增加了16.71%~34.74%。

关键词：砾砂根-土复合体抗剪强度相关分析组分质量