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Neurofilament 200 expression in a rat model of complete spinal cord injury following growth-associated protein-43 treatment

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Neural Regeneration Research 2009年第11期4卷 827-831页

作者： yanping Duan Dongkui Zhang Yingchun Ba Yun yuan Jun Sun Dengli Fu Ran Zhang Jinde Wang Department of Histology and Embryology Kunming Medical University Kunming 650031 Yunnan Province China Yunnan Medical College Kunming Medical University Kunming 650031 Yunnan Province China Department of Anatomy Kunming Medical University Kunming 650031 Yunnan Province China Second Affiliated Hospital Kunming Medical University Kunming 650031 Yunnan Province China

BACKGROUND： Growth-associated protein-43 （GAP-43） expression in the nervous system has been demonstrated to promote neural regeneration, neuronal growth and development, as well as synaptic reconstruction. Neurofilament 200 （NF200） expression could reflect degree of injury and repair in injured spinal axons. OBJECTIVE： To observe NF200 expression changes in a rat model of complete spinal cord injury following GAP-43 treatment and to explore the effects of GAP-43 following spinal cord injury. DESIGN, TIME AND SETTING： A randomized, controlled, animal experiment was performed at the Laboratory of Histology and Embryology of Kunming Medical University between March 2007 and October 2008. MATERIALS： GAP-43 and GAP-43 antibody were provided by Beijing Boao Biology, China; mouse anti-rat NF200 antibody was purchased from Chemicon, USA. METHODS： Female, 8-week-old, Sprague Dawley rats were randomly assigned into three groups following complete spinal cord injury, with 20 animals in each group： GAP-43 antibody, GAP-43, and model groups. In addition, each group was subdivided into four subgroups according to sampling time after modeling, Le., 3-, 5-, 9-, and 15-day groups, with 5 rats in each group. GAP-43 antibody or GAP-43 was injected into injury sites of the spinal cord, 5 μg/0.2 mL, respectively, twice daily for three consecutive days, followed by three additional days of injection, once daily. The model group did not receive any treatment following injury. MAIN OUTCOME MEASURES： NF200 expression in the damaged spinal area at different stages was detected by immunohistochemistry; lower limb motion function following injury was evaluated using the Basso, Beattie and Bresnahan （BBB） locomotor rating scale. RESULTS： NF200 expression was significantly reduced in the GAP-43 antibody group, compared with GAP-43 and model groups, at 3 and 5 days after spinal cord injury （P 〈 0.05）. In addition, the model group expressed significantly less NF200 than the GAP-43 group （P 〈 0.05）. BBB scores from the GAP-43 antibody and model

关键词： spinal cord injury growth-associated protein-43 neurofilament 200 Basso, Beattie and Bresnahan locomotor rating scale

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Polymeric ionic liquid—assisted polymerization for soluble polyaniline nanofibers

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Frontiers of Chemical Science and Engineering 2021年第1期15卷 118-126页

作者： Jie Liu Jiahao Shen Jingjing Wang yuan Liang Routeng Wu Wenwen Zhang Delin Shi Saixiang Shi yanping Wang Yimin Wang Yumin Xia Key Laboratory of High Performance Fibers&Products(Ministry of Education) State Key Laboratory for Modification of Chemical Fibers and Polymer MaterialsCollege of ScienceCollege of Materials Science and EngineeringDonghua UniversityShanghai 201620China Hunan Changsha Aiwanting Home Texitle Products Co.Ltd Changsha 410600China

To enhance the solubility of polyanilines(PANI),polymeric ionic liquid(PIL)was introduced into the polymerization synthesis of PANI with various proportions.The structure and properties of the modified PANIs were characterized by 1H NMR,Fourier transform infrared spectroscopy,thermogravimetric analysis,ultraviolet-visible spectrum,etc.It was found that the obtained PANIs doped with PILs were soluble in various organic solvents such as N,N-dimethyl formamide and acetonitrile.Compared with the pure PANI,the PANIs doped by PILs showed remarkable solubility and their chemical structure and conductivity kept integrated.

关键词： polyaniline polymeric ionic liquid doping solubility

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Inner Envelope CHLOROPLAST MANGANESE TRANSPORTER 1 Supports Manganese Homeostasis and Phototrophic Growth in Arabidopsis

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Molecular Plant 2018年第7期11卷 943-954页

作者： Bin Zhang Chi Zhang Congge Liu yanping Jing yuan Wang Ling Jin Lei Yang Aigen Fu Jisen Shi Fugeng Zhao Wenzhi Lan Sheng Luan Nanjing University-Nanjing Forestry University Joint Institute for Plant Molecular Biology College of Life Sciences Nanjing University Nanjing 210093 China The Key Laboratory of Western Resources Biology and Biological Technology College of Life Sciences Northwest University Xi'an China Nanjing University-Nanjing Forestry University Joint Institute for Plant Molecular Biology Key Laboratory of Forest Genetics and BiotechnologyNanjing Forestry University Nanjing 210037 China Department of Plant and Microbial Biology University of California Berkeley CA 94720 USA These authors contributed equally to this article.

Manganese （Mn） is an essential catalytic metal in the Mn-cluster that oxidizes water to produce oxygen dur- ing photosynthesis. However, the transport protein（s） responsible for Mn2＋ import into the chloroplast re- mains unknown. Here, we report the characterization ofArabidopsis CMT1 （Chloroplast Manganese Trans- porter 1）, an evolutionarily conserved protein in the Uncharacterized Protein Family 0016 （UPFO016）, that is required for manganese accumulation into the chloroplast. CMT1 is expressed primarily in green tissues, and its encoded product is localized in the inner envelope membrane of the chloroplast. Disruption of CMT1 in the T-DNA insertional mutant cmtl-1 resulted in stunted plant growth, defective thylakoid stacking, and severe reduction of photosystem II complexes and photosynthetic activity. Consistent with reduced oxy- gen evolution capacity, the mutant chloroplasts contained less manganese than the wild-type ones. In sup- port of its function as a Mn transporter, CMT1 protein supported the growth and enabled Mn2＋ accumula- tion in the yeast cells of Mn2＋-uptake deficient mutant （3smfl）. Taken together, our results indicate that CMT1 functions as an inner envelope Mn transporter responsible for chloroplast Mn2＋ uptake.

关键词： chloroplast inner envelope manganese transporter metal transport oxygen-evolving complex

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江苏省夏玉米新品种苏科玉218选育研究

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作物研究 2024年第1期38卷 50-54页

作者：郑飞孔令杰孟庆长崔亚坤刘瑞响张美景袁建华陈静陈子恒赵文明陈艳萍江苏省农业科学院粮食作物研究所江苏南京210014

苏科玉218是由江苏省农业科学院粮食作物研究所以自育系JS151040为母本,自育系JS161133为父本杂交选育而成的玉米新品种。该品种丰产稳产性好,抗逆能力强,籽粒品质优良。于2020和2021年参加江苏省夏播区域试验,两年平均产量7849.7 kg/hm... 详细信息

苏科玉218是由江苏省农业科学院粮食作物研究所以自育系JS151040为母本,自育系JS161133为父本杂交选育而成的玉米新品种。该品种丰产稳产性好,抗逆能力强,籽粒品质优良。于2020和2021年参加江苏省夏播区域试验,两年平均产量7849.7 kg/hm^(2),比对照郑单958平均增产12.3%。2022年参加江苏省夏播生产试验,平均产量7714.4 kg/hm^(2),比对照郑单958增产3.8%。2023年通过江苏省农作物品种审定委员会审定,适宜在江苏省北部夏玉米播种区域种植推广。

关键词：玉米育种苏科玉218

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断块油藏动态层系井网合理参数界限及应用——以东辛油区营17断块为例

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石油地质与工程 2024年第1期38卷 84-89页

作者：卢惠东庞丽丽薛巨丰姚娜聂法健王艳平中国石化胜利油田分公司东辛采油厂山东东营257000 中国石化胜利油田分公司CCUS项目部山东东营257000 长江大学石油工程学院湖北武汉430100

东辛油区营17断块受地质因素及开发现状影响,层间矛盾加大,平面干扰加剧,固有流线调控难度加大,亟需开展动态层系井网合理技术政策研究。依托数值模拟手段,通过多方案对比预测,明确分采分注中生产井合理层间级差控制在4以内、注水井同... 详细信息

东辛油区营17断块受地质因素及开发现状影响,层间矛盾加大,平面干扰加剧,固有流线调控难度加大,亟需开展动态层系井网合理技术政策研究。依托数值模拟手段,通过多方案对比预测,明确分采分注中生产井合理层间级差控制在4以内、注水井同一层段内合理级差控制在4~6;对于高含水开发阶段中高渗油藏,井距为400 m时采收率最高;同一层段内注采比为1时,合理换层时机为3个月,随着注采比的增加,换层时间缩短;在矿场实践中,同一层段组合中尽量保持动态参数相近,开发效果也最好。该研究为完善提升技术、扩大应用规模奠定基础,以此技术政策界限为指导,在营17西单元持续开展上段调整应用,年度综合含水下降2.9个百分点,运行成本下降33元/t。研究成果对胜利油田断块储量的高效开发具有重要意义,同时也为胜利油田特高含水阶段效益稳产提供保障。

关键词：动态层系井网纵向差异组合合理级差井距注采比换层时机

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玉米细胞核雄性不育基因的研究进展及其在玉米育种中的应用

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分子植物育种 2022年第12期20卷 3959-3973页

作者：任源林彦萍长春市农业科学院长春130111 吉林工程技术师范学院生物质功能材料交叉学科研究院长春130052

作为重要的农作物之一的玉米不仅是生物燃料、食品、饲料和工业原料的来源,而且还是解决遗传学基本问题的模式植物。雄性不育可以提高杂种优势的利用率和杂交种子的产量。玉米中细胞核雄性不育(genic male-sterility,GMS)基因的鉴定和... 详细信息

作为重要的农作物之一的玉米不仅是生物燃料、食品、饲料和工业原料的来源,而且还是解决遗传学基本问题的模式植物。雄性不育可以提高杂种优势的利用率和杂交种子的产量。玉米中细胞核雄性不育(genic male-sterility,GMS)基因的鉴定和表征可加深对控制花药和花粉发育的分子机制的认识,也可用于开发和有效利用基于生物技术的雄性不育(biotechnology-based male-sterility,BMS)系统进行作物杂交育种。本综述总结了玉米中GMS基因的鉴定和表征的研究进展,并讨论了十几个BMS系统的特征,为繁殖雄性不育系和玉米杂交育种提供了理论基础;最后,对玉米GMS基因研究及其新型BMS系统开发进行了展望。

关键词：核雄性不育雄性不育系统玉米

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变速恒频风力发电系统风速短期预测方法研究

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电网与清洁能源 2022年第2期38卷 88-93页

作者：苏艳萍田源国网宁夏电力有限公司宁夏回族自治区银川750001

采用现有方法预测短期变速恒频风力发电系统的风速时,因未分析风力机的运行特性而导致无法准确预测系统的输出无功功率、输出有功功率和短期风速,且预测结果的平均绝对误差和均方误差大,为此提出变速恒频风力发电系统风速的预测方法。... 详细信息

采用现有方法预测短期变速恒频风力发电系统的风速时,因未分析风力机的运行特性而导致无法准确预测系统的输出无功功率、输出有功功率和短期风速,且预测结果的平均绝对误差和均方误差大,为此提出变速恒频风力发电系统风速的预测方法。首先对风力机的运行特性进行分析,然后采用支持向量机回归算法构建风速预测模型,最后利用风速预测模型完成变速恒频风力发电系统风速的短期预测。实验结果表明,所提方法可准确地预测系统的输出无功功率、输出有功功率和短期风速,且预测结果的平均绝对误差和均方误差小,验证了所提方法的整体有效性。

关键词：变速恒频风力发电系统风速短期测量运行特性支持向量机回归算法

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油脂的风味及感知

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食品与生物技术学报 2022年第6期41卷 13-20页

作者：陈艳萍阿丽雅刘源上海交通大学农业与生物学院上海200240

油脂是一种重要的营养物质。随着我国经济水平的发展,居民越来越多地关注油脂的风味品质。油脂原料本身的差异以及加工制备过程中的脂肪氧化降解、热分解、美拉德反应等是影响油脂形成不同风味品质的主要因素。口腔加工中油脂及其制品... 详细信息

油脂是一种重要的营养物质。随着我国经济水平的发展,居民越来越多地关注油脂的风味品质。油脂原料本身的差异以及加工制备过程中的脂肪氧化降解、热分解、美拉德反应等是影响油脂形成不同风味品质的主要因素。口腔加工中油脂及其制品经过味觉、嗅觉、三叉神经感觉等方面相互作用,在大脑特定区域形成风味的认知。目前有研究证明脂肪酸转位酶(CD36)、G蛋白偶联受体(GPCR)等受体参与到一个专门用于感知脂质的感官系统。因此,有人提出脂肪可能是第6种味道。复杂的多感官交互依赖于人类所有感官的反应,未来关于脂肪感知以及脂肪参与多感官交互方面还需要进一步研究。

关键词：油脂风味口腔加工分子机制

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Online Survey on Accessing Psychological Knowledge and Interventions During the COVID-19 Pandemic-China, 2020

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China CDC weekly 2020年第41期2卷 797-803页

作者： Qingdong Lu Lin Liu Yunhe Wang Le Shi Yingying Xu Zhengan Lu Jianyu Que Jingli Yue Kai yuan Wei Yan Yankun Sun Jie Shi yanping Bao Lin Lu National Institute on Drug Dependence and Beijing Key Laboratory of Drug Dependence Peking UniversityBeijingChina School of Public Health Peking UniversityBeijingChina Institute of Mental Health National Clinical Research Center for Mental DisordersKey Laboratory of Mental Health and Peking University Sixth HospitalPeking UniversityBeijingChina Peking-Tsinghua Center for Life Sciences and PKU-IDG/McGovern Institute for Brain Research Peking UniversityBeijingChina

Summary What is already known on this topic?The public was at elevated risk of mental health illnesses during the coronavirus disease 2019(COVID-19)pandemic,so accessibility to psychological knowledge and interventions is vital to promptly respond to mental health crises.What is added by this report?During the pandemic period,40,724(71.9%)participants reportedly had access to psychological knowledge,and 36,546(64.5%)participants had accessed information on psychological interventions.Participants who were male,unmarried,living alone,divorced or widowed,or infected with COVID-19 were less likely to access psychological knowledge and intervention.

关键词： COV prompt Access

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Effects of Mahuang(Herba Ephedra Sinica) and Wuweizi(Fructus Schisandrae Chinensis) medicated serum on chemotactic migration of alveolar macrophages and inters regions macrophages in rats

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Journal of Traditional Chinese Medicine 2017年第5期37卷 607-615页

作者： yuan Yiping Zhai Huaqiang Zhang Tian Guo Zhaojuan Kong Liting Jia Xiaoyu Wang Ningning Dai Ying Liu Guoxiu Wang yanping Department of Clinical Chinese Pharmacy School of Chinese Materia Medica Beijing University of Chinese Medicine Department of Pharmacy Beijing Hospital Institute of Clinical Basic Medicine of Traditional Chinese Medicine Chinese Academy of Traditional Chinese Medicine

OBJECTIVE: To observe the effects of serum containing Mahuang(Herba Ephedra Sinica) or Wuweizi(Fructus Schisandrae Chinensis) on the migration of alveolar macrophages(AM) and interstitial macrophages(IM) from normal rats, and to analyze and compare the mechanisms leading to cell migration differences.METHODS: Rats were randomly divided into three groups: Mahuang(Herba Ephedra Sinica), Wuweizi(Fructus Schisandrae Chinensis), and blank serum. After treatment with the herbs, serum was extracted from the rats. AM and IM were isolated from normal rats and cultured. The effects of Mahuang(Herba Ephedra Sinica) and Wuweizi(Fructus Schisandrae Chinensis) medicated serum on normal rat AM and IM chemotactic migration were determined by transwell assays. The CC chemokine receptor(CCR) 2, CCR5, voltage-gated Kvl. 3 K^+channel(Kv1. 3), and voltage-gated Kvl. 5 K^+channel(Kv1. 5) protein levels were analyzed by western blotting.RESULTS: The migration quantities of AM and IM in the Mahuang(Herba Ephedra Sinica) and Wuweizi(Fructus Schisandrae Chinensis) medicated serum groups were significantly higher than those in the blank serum group(P < 0.01). Compared with the Wuweizi(Fructus Schisandrae Chinensis) medicated serum group, the migration quantity of cultured rat AM in the Mahuang(Herba Ephedra Sinica) medicated serum group was significantly increased(P <0.01). Meanwhile, compared with the Mahuang(Herba Ephedra Sinica) medicated serum group, the migration quantity of cultured rat IM in the Wuweizi(Fructus Schisandrae Chinensis) medicated serum group was significantly increased(P < 0.01).CCR2, CCR5, Kv1. 3, and Kv1. 5 proteins were expressed on the AM cell surface, and showed significantly higher expression in the Mahuang(Herba Ephedra Sinica) medicated serum group compared with the Wuweizi(Fructus Schisandrae Chinensis)medicated serum group. In contrast, CCR5, Kv1.3,and Kv1.5 proteins were expressed on the IM cell surface, and showed significantly higher expression in the Wuweizi(Fructus Schisandrae Chinensis)medicated serum g

关键词： Ephedra sinica 五味子 Chinensis 巨噬细胞受体 CCR 推迟的整流器钾隧道趋化性

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