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Mid1ip1b modulates apical reorientation of non-centrosomal microtubule organizing center in epithelial cells

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Journal of Genetics and Genomics 2018年第8期45卷 433-442页

作者： Xin Zhou Chun Xiao Yu Li yanna shang Dongqin Yin Siying Li Bo Xiang Ran Lu Yi Ji Yang Wu Wentong Meng Hongyan Zhu Jin Liu Huozhen Hu Xianming Mo Hong Xu Department of Pediatric Surgery and Laboratory of Stem Cell Biology State Key Laboratory of Biotherapy West China Hospital Sichuan University and Collaborative Innovation Center for Biotherapy National Clinical Research Center of Geriatrics West China Hospital Sichuan University

In most kinds of animal cells, the centrosome serves as the main microtubule organizing center （MTOC） that nucleates microtubule arrays throughout the cytoplasm to maintain cell structure, cell division and intracellular transport. Whereas in epithelial cells, non-centrosomal MTOCs are established in the apical domain for generating asymmetric microtubule fibers and cilia in epithelial cells for the organ morphogenesis during embryonic development. However, the mechanism by which MTOCs localize to the apical domain in epithelial cells remains largely unknown. Here, we show that Mid liplb has a close interaction with γ-tubulin protein, the central component of MTOC, and modulates lumen opening of the neural tube, gut, intestine, and kidney of zebrafish. Knockdown or dominant negative effect of Mid 1ip 1 b resulted in failure of lumen formation of the organs as aforementioned. Moreover, the non-centrosomal MTOCs were unable to orientate to the apical domain in Midliplb knockdown epithelial cells, and the centrosomal MTOCs were inaccurately placed in the apical domain, resulting in defective formation of asymmetric microtubules and misplacement of cilia in the apical domain. These data uncover a molecule that controls the proper localization of MTOCs in the apical domain in epithelial cells for organ morphogenesis during embryonic development.

关键词： Mid1ip1b Microtubule organizing center Non-centrosomal MTOC Microtubule Epithelial cell

Neurons generated from carcinoma stem cells support cancerprogression

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Signal Transduction and Targeted Therapy 2017年第1期2卷 320-329页

作者： Ran Lu Chuanwen Fan Wenqi shangguan Yuan Liu Yu Li yanna shang Dongqin Yin Shengliang Zhang Qiaorong Huang Xue Li Wentong Meng Hong Xu Zongguang Zhou Jiankun Hu Weimin Li Lunxu Liu Xianming Mo Laboratory of Stem Cell Biology State Key Laboratory of Biotherapy/Collaborative Innovation Center of BiotherapyWest China HospitalSichuan UniversityChengduChina Institute of Digestive Surgery State Key Laboratory of Biotherapy/Collaborative Innovation Center of BiotherapyWest China HospitalSichuan UniversityChengduChina Department of Gastrointestinal Surgery State Key Laboratory of Biotherapy/Collaborative Innovation Center of BiotherapyWest China HospitalSichuan UniversityChengduChina Laboratory of Gastric Cancer State Key Laboratory of Biotherapy/Collaborative Innovation Center of BiotherapyWest China HospitalSichuan UniversityChengduChina Department of Respiratory and Critical Care Medicine State Key Laboratory of Biotherapy/Collaborative Innovation Center of BiotherapyWest China HospitalSichuan UniversityChengduChina Department of Thoracic Surgery State Key Laboratory of Biotherapy/Collaborative Innovation Center of BiotherapyWest China hospitalSichuan UniversityChengduChina.

Recent evidences show that nervous system acts as a crucial part of cancer *** of nerve fibers into cancer microenvironment has an important active role in cancer *** stimulations of both cancer growth and metastasis by members of nervous system such as neurons and glial cells have been ***,how the nervous system is built in cancer is largely *** we show that a fraction of cancer stem cells(CSCs)derived from patients with gastric carcinoma and colorectal carcinoma are capable of producing neurons that are involved in tumor neurogenesis and tumor *** stem cell monoclone derived from a single cancer stem cell was able to generate neurons including sympathetic and parasympathetic neurons to take part in the nervous system in cancer *** down the neural cell generating capability of the human CSCs inhibited the growth of xenograft tumors in mouse *** data demonstrate that human CSCs are able to produce one of most important components in the cancer microenvironment that are required for cancer development and progression.

关键词： cancer inhibited stimulation

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地铁车站施工对既有高架的保护技术研究

建筑施工 2021年第7期43卷 1362-1363,1375页

作者：尚艳娜上海市政工程设计研究总院(集团)有限公司上海200092

以上海轨交15号线古北路站深基坑工程为例,研究了新建轨道交通车站基坑开挖及降水对既有构筑物结构安全的影响。为确保深基坑施工的安全及既有构筑物的正常使用,从基坑围护结构、地基加固、施工监测等几个方面阐述了对既有构筑物的保护... 详细信息

以上海轨交15号线古北路站深基坑工程为例,研究了新建轨道交通车站基坑开挖及降水对既有构筑物结构安全的影响。为确保深基坑施工的安全及既有构筑物的正常使用,从基坑围护结构、地基加固、施工监测等几个方面阐述了对既有构筑物的保护措施。相关研究结果可供类似工程参考。

关键词：地铁车站既有高架深基坑保护措施

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大型结构体裂缝检测中的定位方法

仪器仪表学报 2017年第3期38卷 681-688页

作者：尚砚娜石晶欣赵岩朱君尧蔡友发北京京仪仪器仪表研究总院有限公司北京100079 天津大学精密测试技术及仪器国家重点实验室天津300072

为了提高大型结构体表面裂缝检测的效率,提出了一种基于图像处理技术和坐标映射相结合的定位方法。该方法首先在序列图像中抽取清晰度最高的一帧,应用Retinex算法对光照引起的亮度不均进行补偿。然后将提取的标志点图像坐标映射到观测... 详细信息

为了提高大型结构体表面裂缝检测的效率,提出了一种基于图像处理技术和坐标映射相结合的定位方法。该方法首先在序列图像中抽取清晰度最高的一帧,应用Retinex算法对光照引起的亮度不均进行补偿。然后将提取的标志点图像坐标映射到观测坐标系下,根据标志点的观测坐标和世界坐标计算两坐标系间的映射关系。再根据形态学图像处理中的凸壳、像素化、细化算法提取裂缝的图像坐标;如果观测新裂缝,则将裂缝图像坐标映射到世界坐标系下;如果观测历史裂缝,则将该条裂缝的世界坐标映射到当前观测坐标系下,并计算该条历史裂缝当前的观测角度,从而实现裂缝的空间定位。经实验证明,该方法高效、准确、便捷,16 s内可实现自动定位,且偏差不大于0.07°。

关键词：裂缝定位图像处理结构体表面空间坐标映射

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浅谈化工装置中仪表线路的工程设计

仪器仪表用户 2022年第4期29卷 105-109页

作者：李国运尚彦娜浙江省天正设计工程有限公司杭州310000

针对化工装置仪表线路所包含的仪表电缆、防爆电缆接头、现场仪表接线箱等组成部分的分类及选型进行了阐述。对仪表线路不同的敷设方式之间的优缺点进行了剖析,提出了电缆槽架空敷设方式是目前在化工装置中比较理想的敷设方式。根据规... 详细信息

针对化工装置仪表线路所包含的仪表电缆、防爆电缆接头、现场仪表接线箱等组成部分的分类及选型进行了阐述。对仪表线路不同的敷设方式之间的优缺点进行了剖析,提出了电缆槽架空敷设方式是目前在化工装置中比较理想的敷设方式。根据规范要求并结合工程实际,提出了现今比较成熟可靠的仪表线路工程设计方案,并提出了仪表线路工程设计中的注意事项。

关键词：仪表电缆化工装置工程设计仪表线路电缆敷设

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静力触探技术在水平钻孔注浆效果评价中的应用

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建井技术 2021年第5期42卷 29-32页

作者：于宁尚艳娜李方政王桦李生生上海申通地铁集团有限公司上海201103 上海市政工程设计研究总院(集团)有限公司上海200092 北京中煤矿山工程有限公司北京100013 天地科技股份有限公司北京100013

针对某市轨道交通地铁隧道工程水平钻孔注浆效果评价难的问题,运用静力触探技术,判断富水区域注浆前后的土体物理性质变化。检测结果发现,静力触探技术可获得试验区域注浆前后的比贯入阻力P_(s)值。注浆后,土体的P_(s)值出现不同程度的... 详细信息

针对某市轨道交通地铁隧道工程水平钻孔注浆效果评价难的问题,运用静力触探技术,判断富水区域注浆前后的土体物理性质变化。检测结果发现,静力触探技术可获得试验区域注浆前后的比贯入阻力P_(s)值。注浆后,土体的P_(s)值出现不同程度的提高。不同土层注浆后,P_(s)值提高率大小排序:灰色淤泥质黏土>淤泥质粉质黏土>灰色黏土>粉质黏土>素填土。注浆后,地基承载力基本值f_(0)、不排水抗剪强度基本值C_(u)、压缩模量基本值E_(s)等土体物理参数,均有不同程度的提高。

关键词：静力触探水平钻孔注浆注浆效果地铁隧道

Quantum Dot Nanobeads-Labelled Lateral Flow Immunoassay Strip for Rapid and Sensitive Detection of Salmonella Typhimurium Based on Strand Displacement Loop-Mediated Isothermal Amplification

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Engineering 2022年第12期19卷 62-70页

作者： Yuting shang Shuzhen Cai Qinghua Ye Qingping Wu yanna Shao Xiaoying Qu Xinran Xiang Baoqing Zhou Yu Ding Moutong Chen Liang Xue Honghui Zhu Jumei Zhang Guangdong Provincial Key Laboratory of Microbial Safety and Health&State Key Laboratory of Applied Microbiology Southern China&Institute of Microbiology Guangdong Academy of SciencesGuangzhou 510070China State Key Laboratory of Food Science and Technology&National Engineering Research Center for Functional Foods&Synergetic Innovation Center of Food Safety&Joint International Research Laboratory on Food Safety School of Food Science and TechnologyJiangnan UniversityWuxi 214122China

Rapid,sensitive,point-of-care detection of pathogenic bacteria is important for food *** this study,we developed a novel quantum dot nanobeads-labelled lateral flow immunoassay strip(QBs-labelled LFIAS)combined with strand displacement loop-mediated isothermal amplification(SD-LAMP)for quantitative Salmonella Typhimurium(ST)*** dot nanobeads(QBs)served as fluorescence reporters,providing good detection *** customizable strand displacement(SD)probe was used in LAMP to improve the specificity of the method and prevent by-product *** was based on a sandwich immunoassay.A fluorescence strip reader measured the fluorescence intensity(FI)of the test(T)line and control(C)*** linear detection range of the strip was 10^(2)–10^(8) colony forming units(CFU)·mL^(-1).The visual limit of detection was 10^(3) CFU·mL^(-1),indicating that the system was ten-fold more sensitive than AuNPs-labelled test *** specificity was analyzed in accordance with agarose gel outputs of polymerase chain reaction(PCR)and *** detected ST in foods with an acceptable recovery of 85%–110%.The method is rapid,simple,almost equipment-free,and suitable for bacterial detection in foods and for clinical diagnosis.

关键词： Salmonella Typhimurium Quantum dot nanobeads Lateral flow immunoassay strip Loop-mediated isothermal amplification Strand displacement probe

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两层流中二维箱体结构反射和透射的模拟研究

哈尔滨工程大学学报 2016年第5期37卷 653-659页

作者：尚玉超勾莹滕斌郑艳娜大连理工大学海岸和近海工程国家重点实验室辽宁大连116024 大连海洋大学海洋与土木工程学院辽宁大连116023

基于两层流体简化模型,研究了水面箱型结构在两层流体中对波浪的反射和透射特性。为研究复杂海洋结构,采用外域级数展开和内域边界元数值方法相结合的手段建立了频域数学模型,在外域和内域交界的辐射面上利用连续性条件建立联立方程组... 详细信息

基于两层流体简化模型,研究了水面箱型结构在两层流体中对波浪的反射和透射特性。为研究复杂海洋结构,采用外域级数展开和内域边界元数值方法相结合的手段建立了频域数学模型,在外域和内域交界的辐射面上利用连续性条件建立联立方程组。其中内域计算采用Rankine源作为格林函数,既避免了求解复杂的格林函数,又可直接获得自由水面及内界面上的速度势。经过与级数展开的解析方法对比,以及两层流中波能流守恒的验证,证明了本文建立的这一联合数值模型的正确性。利用这一数值模型通过计算反射系数和透射系数的变化情况,研究了两层流中存在箱型结构时各模态波浪的转化情况,分析了两层流中箱型结构的反射和透射特性。

关键词：两层流体边界元方法 Rankine源反射系数透射系数水波箱型结构

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自适应滤波在火炮快速定向系统中的应用

弹箭与制导学报 2011年第4期31卷 121-123页

作者：廖延娜商锋西安邮电学院西安710121

为了实现火炮快速定向,文中以卫星信号为参照,给出了在一定基线下,利用天线的"和差方向图"进行炮口定向的基本原理。论述了自适应滤波在"差信号"最小点计算过程中的应用。仿真结果表明,自适应滤波可有效抑制"差信号"中的噪声,从而可以利... 详细信息

为了实现火炮快速定向,文中以卫星信号为参照,给出了在一定基线下,利用天线的"和差方向图"进行炮口定向的基本原理。论述了自适应滤波在"差信号"最小点计算过程中的应用。仿真结果表明,自适应滤波可有效抑制"差信号"中的噪声,从而可以利用"和差方向图"实现较为准确的快速定向。

关键词：卫星信号火炮定向自适应滤波噪声抑制