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Studies on the overexpression of the soybean GmNHX1 in Lotus corniculatus: The reduced Na^+ level is the basis of the increased salt tolerance

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Chinese Science Bulletin 2006年第11期51卷 1306-1315页

作者： SUN Yanxiang WANG Dan bai yanling WANG Ningning WANG Yong College of Life Sciences Nankai University Tianjin 300071 China Tianjin Research Center of Agricultural Biotechnology Tianjin 300183 China Department of Biology Teachers College of Langfang Langfang 065000 China

The full length cDNA coding for a novel vacuolar Na+/H+ antiporter (GmNHX1) was cloned from soybean and determined to consist of 2591 bp with a 5′-untranslated region of 464 bp, an open reading frame (ORF) of 1641 bp, and a 3′-untrans- lated region of 486 bp. The deduced protein se- quence contains 546 aa with the typical characters of the vacuolar Na+/H+ antiporters, and shares high similarity with that of AtNHX1, OsNHX1 and AgNHX1. The soybean genome showed a single copy of the GmNHX1 gene. Northern blot analysis demonstrated that the expression of the GmNHX1 was tissue-spe- cific, increased by ABA treatment, NaCl, KCl, LiCl and dehydration stress, and lower in leaves but higher in roots and hypocotyls of salt-tolerant than salt-sensitive cultivars. The GmNHX1 was overex- pressed under the control of a tandem cauliflower mosaic virus (CaMV) 35S promoter in the model le- guminous plant Lotus corniculatus L. and conferred salt-tolerance of the transgenic plants. Measure- ments of Na+ and K+ contents in both roots and shoots demonstrated that the plantlets of GmNHX1- overexpressing lines had lower Na+ and K+ content, and higher K+/Na+ ratio than the controlled lines that were transformed with the empty vector, which indi- cates that the salt-tolerance conferred by GmNHX1 is closely related with decreased accumulation of Na+ in the transgenic plants.

关键词：大豆 GmNHX1 莲耐盐性盐胁迫

Properties and nucleotide sequence of linear plasmid-like DNA pC4 from mitochondria of Cucumis sativus

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Chinese Science Bulletin 2001年第15期46卷 1288-1291页

作者： Junying Li Yingda Wen yanling bai Juan Fu Xiuming Zhang Caichang Gao Nankai Univ Inst Mol Biol Tianjin 300071 Peoples R China

Four kinds of mitochondrial plasmid-like DNAs, designated pC1, pC2, pC3 and pC4, were detected in Cucumis sativus Jinyan No. 4. The electron microscopy observation showed that pC4 was linear conformation. Complete sequence of pC4 was cloned into pUC19 with E. coli JM109 as host. Sequence analysis revealed that pC4 was 370 bp long, the shortest one among all the reported mitochondrial plasmid-like DNAs. pC4 was AT rich. It contained terminal direct repeat sequence (35 bp in length)as well as many short direct and inverted repeats. ORFs in pC4 were short. pC4 was found to be homologous to nuclear DNAs, but lack homology with main mitochondrial and chloroplast DNAs. pC4-homologous sequence also occurred in nuclear genome of Jinyan No. 7 which contained no mitochondrial plasmid-like DNAs. The hybridization pattern of Jinyan No. 7 was slightly different from that of Jinyan No. 4. This suggested that pC4 occurred at the forepart of Cucumis sativus species divergence and integrated into the nuclear genome,

关键词： Cucumis sativus linear mitochondrial plasmid-like DNAs nucleotide sequence homology.

Development of Multi-purpose Small-sized Rotary Tiller for Greenhouses

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Journal of Northeast Agricultural University(English Edition) 1998年第1期5卷 48-54页

作者： Mi Bolin, Tu Chenghai, Guan yanling, Li bai, Zheng Qiumei (Northeast Agricultural University, Harbin 150030, P R C)

IGLP-62 multi-purpose small-sized rotary tiller is a tool of production urgently needed for “Vegetable Supplying System”in China. It is mainly used for rotary cultivation as well as for fertilizer mixing and stubble mulching in greenhouses. And it can be used for open vegetable plots and orchards as well. The machine is characterized by the integration of rotary tilling with driving. It has a good performance of clod crushing and high capacity. It is equipped with a power-take-off shaft to drive a water pump, thresher or rice mill. Thus the machine solved the long-standing problem of heavy manual labour in greenhouses. So it is a kind of ideal hand cultivation machinery of horticultural type.

关键词： rotary tiller gearbox greenhouse

Expression of Leghemoglobin in Chloroplast to Improve Hydrogen Production in C. reinhardtii

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Expression of Leghemoglobin in Chloroplast to Improve Hydrog...

Epidemiological characteristics of ventilator-associated pneumonia in neurosurgery:A 10-year surveillance study in a Chinese tertiary hospital

2010全国植物生物学研讨会

作者： Qing Liu yanling bai Yerong Zhu Yong Wang College of life sciences Nan Kai University

The benefits of hydrogen as an energy carrier have long been *** a fuel,hydrogen gas is gererally manufactured ***,H2can also be recognized as a kind of biofuel,as many bacteria and some photosynthetic green algae,

关键词： hydrogen production Chlamydomonas reinhardtii leghemoglobin genetic transformation

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Infectious Medicine

Infectious Medicine 2024年第3期3卷 1-9页

作者： Zhenghao Yu Xinlou Li Chenglong Lv Yao Tian Jijiang Suo Zhongqiang Yan yanling bai Bowei Liu Liqun Fang Mingmei Du Hongwu Yao Yunxi Liu Department of Disease Prevention and Control the First Medical CenterChinese PLA General HospitalBeijing 100853China Medical School of Chinese PLA Beijing 100853China Department of Medical Research Key Laboratory of Environmental Sense Organ Stress and Health of the Ministry of Environmental Protectionthe Ninth Medical CenterChinese PLA General HospitalBeijing 100101China State Key Laboratory of Pathogen and Biosecurity Beijing Institute of Microbiology and EpidemiologyBeijing 100071China

Background Ventilator-associated pneumonia(VAP)is a significant and common health *** epidemiological landscape of VAP is poorly understood in neurosurgery *** study aimed to explore the epidemiology of VAP in this population and devise targeted surveillance,treatment,and control *** A 10-year retrospective study spanning 2011 to 2020 was performed in a large Chinese tertiary *** data was collected from neurosurgical patients and analyzed to map the demographic and clinical characteristics of VAP and describe the distribution and antimicrobial resistance profile of leading *** factors associated with the presence of VAP were explored using boosted regression tree(BRT)*** Three hundred ten VAP patients were *** 10-year incidence of VAP was 16.21 per 1000 ventilation ***-cause mortality was 6.1%.The prevalence of gram-negative bacteria,fungi,and gram-positive bacteria among the 357 organisms isolated from VAP patients was 86.0%,7.6%,and 6.4%,respectively;most were multidrug-resistant *** baumannii,Klebsiella pneumoniae,and Pseudomonas aeruginosa were the most common *** prevalence of carbapenem-resistant ***,***,and *** was high and increased over time in the study *** BRT models revealed that VAP was associated with number of days of ventilator use(relative contribution,47.84±7.25),Glasgow Coma Scale score(relative contribution,24.72±5.67),and tracheotomy(relative contribution,21.50±2.69).Conclusions Our findings provide a better understanding of the epidemiology of VAP and its risk factors in neurosurgery patients.

关键词： Mechanical ventilation pneumonia Epidemiological characteristic Neurosurgery Multi-drug resistance Risk factor

天津近代传统合院式民居遗存现状调查及保护对策

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现代城市研究 2016年第2期31卷 120-125页

作者：白艳玲天津科技大学法政学院

传统合院式民居曾是天津地区最主要的住宅形式,但时至今日历经城市改造、村镇建设,大量旧有民居被拆除。目前,天津地区具有重要历史文化价值的传统合院式民居保存数量极少,整体保护情况堪忧。本课题组经过多次实地调查、广泛搜集资料,... 详细信息

传统合院式民居曾是天津地区最主要的住宅形式,但时至今日历经城市改造、村镇建设,大量旧有民居被拆除。目前,天津地区具有重要历史文化价值的传统合院式民居保存数量极少,整体保护情况堪忧。本课题组经过多次实地调查、广泛搜集资料,调查了天津地区具有百年以上历史的近代传统合院式民居的现状,并整理出其遗存的基本情况。以期为保存天津民居资料贡献一份力量,为天津建设城市特色文化与城市环境整体规划提供辅助性参考。

关键词：天津合院式民居遗存现状保护对策

矿山井筒视频图像实时配准的改进Sift算法

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金属矿山 2018年第7期47卷 163-166页

作者：白艳玲郑州铁路职业技术学院软件学院河南郑州451460

针对传统矿山井筒视频图像在匹配过程中存在的匹配精度低、实时性不强的问题,提出了一种矿山井筒视频图像配准的改进Sift算法。该算法以Harris角点检测代替Sift算法的极值检测,首先构建高斯尺度空间,提取满足尺度不变形要求的角点特征;... 详细信息

针对传统矿山井筒视频图像在匹配过程中存在的匹配精度低、实时性不强的问题,提出了一种矿山井筒视频图像配准的改进Sift算法。该算法以Harris角点检测代替Sift算法的极值检测,首先构建高斯尺度空间,提取满足尺度不变形要求的角点特征;然后采用Forsnter算子对提取点进行精确定位,并基于Sift算法的特征描述方式对提取点进行描述;最后利用Ransac法和随机K-D树完成特征点匹配。试验结果表明:改进Sift算法的匹配精度优于优传统Sift算法,可以大幅缩短矿山井筒视频图像配准时间,有助于实现矿山井筒视频图像实时匹配。

关键词：矿山井筒视频图像 Sift算法 Harris算子实时配准

Large scale genome deletion in budding yeast Saccharomycescerevisiae

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Large scale genome deletion in budding yeast Saccharomycesce...

In vitro and in vivo Immunomodulatory Activity of Polysaccharide from Gynostemma pentaphyllum Makino

中国生物化学与分子生物学会第十一次会员代表大会暨2014年全国学术会议

作者： Quanli Liu Yuzhen Wu Yatian Hong Rui Yang Xiuming Zhang yanling bai Mingqiang Qiao Department of Microbiology College of Life Sciences Nankai University The Key Laboratory of Molecular Microbiology and Technology Ministry of Education Nankai University

The concept of minimum genome factories(MGFs) has been proposed, which can bedefined as recombinant strains whose metabolism has been streamlined to the optimalminimal subset in order to maximize product formation for targeted applications. Theplasticity of genomes gives us hints that it is probable to streamline physiological pathwaysby means of genome rearrangements, as cells harbor a number of genes that onlymarginally contribute to cellular fitness and various dispensable activities that are not essentialfor survival, particularly when cells are grown in rich media. The unicellulareukaryotebudding yeast Saccharomyces cerevisiae has long been exploited for industrial andbiotechnological applications and is extensively chosen as the starting platform for valueaddedmetabolites biosynthesis and biofuel production. Construction of a set of chassiscells provides the opportunity to obtain specific target strain(s) with desired performanceand thus represents the fundamental work for the generation of featured *** proposeda new scarless modification strategy, which evolved from a recently establishedmazF-based gene deletion protocol, for large-scale and scarless genome rearrangementsin yeast S. cerevisiae. The deletion cassette is composed of five functional parts: a URA3 marker for integrant selection, a galactoseinduced mazF cassette for counter-selectionand three genome segments. Each part was amplified by PCR and co-transformed intoyeast cells in which the complete deletion cassettes were assembled through homologousrecombination among the 50 bp overlaps introduced in primers. Homologous recombinationamong two flanking homology regions and their counterparts in the genome resultedin the targeted integration of deletion cassettes and created new direct repeats of thethird interior genome sequence. Integrants were selected on SC-Ura plates and successfulintegrations were verified by direct colony PCR. The resultant cells were subjected tocounter-selection step to a

关键词： Saccharomyces cerevisiae MGFs

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In vitro and in vivo Immunomodulatory Activity of Polysaccha...