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饲喂不同种类粗饲料的辽宁绒山羊瘤胃酶活性及气体组成的研究

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现代畜牧兽医 2013年第5期 30-33页

作者：孙亚波边革刘玉英孙宝成佟辉邵孟跃赵艳娇辽宁省畜牧科学研究院辽宁辽阳111000 辽宁省辽宁绒山羊原种场有限公司辽宁盖州115200

本研究选择9只年龄3.2±0.2岁、体重50.4±2.6kg的装有永久性瘤胃瘘管的成年公羊为试验动物。采用随机区组设计,将动物分为3组,一次饲喂给以玉米秸、大豆秸、花生秸为主的1、2、3号试验日粮,每天8:00和16:00饲喂,全天自由饮水。试验预试... 详细信息

本研究选择9只年龄3.2±0.2岁、体重50.4±2.6kg的装有永久性瘤胃瘘管的成年公羊为试验动物。采用随机区组设计,将动物分为3组,一次饲喂给以玉米秸、大豆秸、花生秸为主的1、2、3号试验日粮,每天8:00和16:00饲喂,全天自由饮水。试验预试期15d,试验期15d,连续7d采集瘤胃内容物和瘤胃气体,研究瘤胃发酵气体组成和瘤胃内各种酶的活性。结果表明:羧甲基纤维素酶活性范围在11.63～12.57mol/***,纤维二糖酶活性范围在1.97～2.39mol/***,木糖酶活性范围在22.75～24.84mol/***,总脱氢酶活性范围在4.72～5.31u/ml。其中试验1组的以上四种酶活性均高于试验2组和试验3组。瘤胃发酵的气体组成中,CH4的比例为16.70%～18.29%,CO2比例为5.67%～14.75%,O2比例为2.13%～4.17%,N2比例为61.95%～75.48%。其中试验1组CH4和CO2比例均高于试验2组和试验3组,但差异不显著(P>0.05);而试验1组氮气比例极显著(P<0.01)低于试验2组和3组。因此,饲喂玉米秸秆的瘤胃内纤维素降解酶活性高,甲烷和二氧化碳产量大,有机物质消化降解较好。

关键词：粗饲料辽宁绒山羊酶活性瘤胃气体组成

EDTA-2NA对辽宁绒山羊精液低温保存效果的影响试验

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畜牧兽医科学（电子版） 2021年第18期 25-27页

作者：杨秋凤张兴会刘兴伟张晓鹰赵艳娇佟玉红黄国卫辽宁省现代农业生产基地建设工程中心辽宁辽阳111000 辽宁省农业发展服务中心辽宁沈阳111000 辽宁省朝阳市建平县动物卫生监督所辽宁朝阳122400

本试验旨在研究绒山羊精液低温保存稀释液中添加EDTA-2Na对其精液品质的影响。假阴道法采集活率不低于70%的绒山羊精液进行混合,在稀释液中分别添加EDTA-2Na的浓度为对照组0%、试验1组0.05%、试验2组0.1%、试验3组0.15%,在4℃环境下保存... 详细信息

本试验旨在研究绒山羊精液低温保存稀释液中添加EDTA-2Na对其精液品质的影响。假阴道法采集活率不低于70%的绒山羊精液进行混合,在稀释液中分别添加EDTA-2Na的浓度为对照组0%、试验1组0.05%、试验2组0.1%、试验3组0.15%,在4℃环境下保存,每隔24 h检测精子活率、顶体完整率。结果表明,稀释液中添加ETDA-2Na在维持精子活率、顶体完整率、和降低畸形率方面效果最好,并且0.15%EDTA-2Na处理组好于添加0.05%、0.1%EDTA-2Na的效果。

关键词： EDTA-2Na 精液稀释液低温保存

新疆农牧区老年肌少症患者外周血PBMC中TWEAK、Fn14及NF-κB通路基因表达分析

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山东医药 2023年第32期63卷 25-29页

作者：赵艳姣刘金玲卓娅·买买提乌斯满冯智群杨星雨王红梅新疆医科大学研究生学院乌鲁木齐830000 新疆维吾尔自治区人民医院综合保健内科二病区石河子大学医学院

目的观察新疆农牧区老年肌肉减少症(后简称肌少症)患者外周血单个核细胞(PBMC)中肿瘤坏死因子样细胞凋亡弱诱导物(TWEAK)、成纤维细胞生长因子诱导早期反应蛋白14(Fn14)及核因子κB(NF-κB)通路基因的表达变化,并探讨其意义。方法在新... 详细信息

目的观察新疆农牧区老年肌肉减少症(后简称肌少症)患者外周血单个核细胞(PBMC)中肿瘤坏死因子样细胞凋亡弱诱导物(TWEAK)、成纤维细胞生长因子诱导早期反应蛋白14(Fn14)及核因子κB(NF-κB)通路基因的表达变化,并探讨其意义。方法在新疆农牧区常住居民中选择80例研究对象,肌少症患者40例纳入肌少症组、健康人群40例纳入对照组。提取两组空腹外周血中的PBMC,采用qRT-PCR法检测TWEAK、Fn14、IκB激酶α(IKKα)、IκB激酶β(IKKβ)、NF-κB p65 mRNA。结果肌少症组外周血PBMC中TWEAK、Fn14、IKKα、IKKβmRNA表达高于对照组(P均<0.05)。校正性别、年龄后,协方差分析结果仍显示,肌少症组外周血PBMC中TWEAK、Fn14、IKKα、IKKβmRNA表达高于对照组(P均<0.05)。校正年龄、性别后,偏相关性分析显示,TWEAK mRNA与Fn14 mRNA表达呈正相关(r=0.424,P<0.01),Fn14 mRNA与IKKα、IKKβmRNA表达呈正相关(r分别为0.264、0.284,P均<0.05),IKKα、IKKβmRNA与NF-κB p65 mRNA表达呈正相关(r分别为0.724、0.660,P均<0.01)。结论新疆农牧区老年肌少症患者外周血PBMC中TWEAK、Fn14、IKKα、IKKβmRNA高表达,TWEAK、Fn14 mRNA表达与IKKα、IKKβmRNA表达有相关性;TWEAK、Fn14可能通过调节IKK激活NF-κB炎症通路从而参与老年人肌少症的发生发展。

关键词：肌肉减少症 TWEAK基因 Fn14基因 TWEAK/Fn14轴 NF-κB通路老年人新疆地区

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轻便防寒保暖鞋靴工艺制作及性能表征

纺织检测与标准 2023年第5期9卷 1-4页

作者：赵艳娇张慧霞卜婷婷李茹茹王龙石倩王大伟刘丽芳东华大学纺织学院上海201620 际华集团股份有限公司系统工程中心北京100160 际华三五一四制革制鞋有限公司河北石家庄050081 北京邦维高科新材料科技股份有限公司北京100043

为开发适应恶劣或极端气候条件的作业人员的脚部防护产品,以轻量化、保暖性、专业性、防护性和舒适性为主要设计原则,选用羊毛、牛皮等材质,通过改变保暖材料层次,增设外置骨架结构,设计了机动作业时多地形穿用的轻便防寒保暖鞋靴,以提... 详细信息

为开发适应恶劣或极端气候条件的作业人员的脚部防护产品,以轻量化、保暖性、专业性、防护性和舒适性为主要设计原则,选用羊毛、牛皮等材质,通过改变保暖材料层次,增设外置骨架结构,设计了机动作业时多地形穿用的轻便防寒保暖鞋靴,以提升作业人员的脚踝防护性。测试结果表明,该鞋靴轻便,具有较好的保暖性、成鞋耐折性能、外底耐磨性能和成鞋抗刺穿力。

关键词：轻便防寒保暖脚部防护

Microencapsulated Lactobacillus plantarum promotes intestinal development through gut colonization of layer chicks

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Animal Nutrition 2024年第3期18卷 1-16页

作者： Yaoming Cui Yanxia Liu Jing Yang Haitao Duan Peng Wang Linna Guo yanjiao Guo Suying Li Yating zhao Jinrong Wang Guanghai Qi Junjun Guan School of Biological Engineering Henan University of TechnologyZhengzhouHenan 450001China College of Animal Science and Technology Henan University of Animal Husbandry and EconomyZhengzhouHenan 450046China Feed Research Institute Chinese Academy of Agricultural SciencesBeijing 100081China

The effects of Lactobacillus plantarum in microencapsulation(LPM)on intestinal development in layer chicks were investigated in this study,as well as the colonization of *** in the gut.A total of 480 healthy Hy-Line Brown layer chicks at 0 d old were randomly divided into 4 groups(8 replicates each treatment),and the diets of these birds were supplemented with nothing(control),***(0.02 g/kg feed;10^(9) CFU/kg feed),LPM(1.0 g/kg feed;109 CFU/kg feed)and wall material of LPM(WM;0.98 g/kg feed),*** to control,LPM improved growth performance and intestinal development of layer chicks,evidenced by significantly increased body weight,average daily gain,average daily feed intake,villus height,villus height/crypt depth,as well as weight and length of the duodenum,jejunum and ileum(P<0.05).These results could be attributed to the increased colonization of *** in the gut,which was verified by significant increases in lactic acid content,viable counts in chyme and mucosa(P<0.05),as well as a visible rise in number of strains labeled with fluorescein ***,the relative abundances of Lactobacillus and Bifidobacterium significantly increased in response to microencapsulated *** supplementation(P<0.05),accompanied by the significant up-regulation of colonization related genes(P<0.05),encoding solute carrier family,monocarboxylate transporter,activin A receptor,succinate receptor and secretogranin *** sum up,microencapsulated *** supplementation promoted intestinal development,which could be attributed to the enhancement of *** colonization in the intestine through the mutual assistance of Bifidobacterium and interactions with colonization related transmembrane proteins.

关键词： Intestinal development Colonization Microbiota Layer chick Lactobacillus plantarum

Influence of Dietary Probiotic Bacillus TC22 and Prebiotic Fructooligosaccharide on Growth,Immune Responses and Disease Resistance against Vibrio splendidus Infection in Sea Cucumber Apostichopus japonicus

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Journal of Ocean University of China 2011年第3期10卷 293-300页

作者： zhao Yancui MAI Kangsen XU Wei ZHANG Wenbing AI Qinghui ZHANG yanjiao WANG Xiaojie LIUFU Zhiguo The Key Laboratory of Mariculture Ministry of Education Ocean University of China Qingdao 266003 P. R. China

The effects of probiotic Bacillus TC22 (isolated from intestine of infected sea cucumber) and prebiotic fructooligosaccharide (FOS) on growth, immunity and disease resistance in sea cucumber Apostichopus japonicus were studied. Six experimental diets were formulated with combinations of three levels of TC22 (0, 107 and 109 CFU g-1 diet) and two levels of FOS (0 and 0.5%) in a 3 × 2 factorial experiment. At the end of the 8-week feeding trial,animals were challenged by injecting Vibrio splendidus. The results revealed that the specific growth rates (SGR) of sea cucumbers were not affected by TC22 and FOS, or the interaction between TC22 and FOS (P > 0.05). However,there were significant interactions between TC22 and FOS for immune response and disease resistance in sea cucumbers (P 0.05). Therefore, further studies should examine the effects of combinations of other levels of FOS(> 0.5% or < 0.5%) and TC22 on the immunity and disease resistance of sea cucumbers.

关键词： Apostichopus japonicus probiotics prebiotics immunity Vibrio splendidus

Research Progress of Maternal Environmental Effects in Resource-based Regions

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Plant Diseases and Pests 2020年第4期11卷 31-35页

作者： Li yanjiao Tong Weishuang Gao Huashan zhao Yan Wang Limin Pingdingshan University Key Laboratory of Ecological Restoration in Hilly AreaPingdingshan 467000China Forestry College Henan University of Science&TechnologyLuoyang 471003China

Heavy metal pollution is common in resource-based ***,the morphological,physiological and biochemical mechanisms of plants transmitting environmental information to their offspring in environments polluted by heavy metals have not been studied *** soybean,an annual self-pollinated plant in Huang-Huai-Hai region,as the research object,the morphological,physiological and biochemical indexes and heavy metal enrichment and distribution characteristics of plants under heavy metal stress were monitored continuously for about three generations,and the trans-generational differences of soybean reproduction,vegetative growth,stress resistance and life cycle in heavy metal stress environment were *** results will be helpful to better understand the long-term adaptive strategy of plants to heavy metal stress and provide the theory basis for ecological security of soybean and other crops under heavy metal stress.

关键词： Maternal environmental effect Glycine max(L.)Merr. Heavy metal stress Plant life history Resource-based regions Transgenerational plasticity

Characteristics of Soil Seed Banks of Typical Plant Communities in Hilly Area of Funiu Mountain

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Asian Agricultural Research 2020年第11期12卷 27-33页

作者： yanjiao LI Pengsen CAI Weishuang TONG Huashan GAO Yan zhao Limin WANG Key Laboratory of Ecological Restoration in Hilly Area Pingdingshan University Pingdingshan 467000 China College of Horticulture and Plant Protection Henan University of Science and Technology Luoyang 471003 China

[Objectives]The purpose was to investigate the characteristics of soil seed bank of typical plant communities in hilly area of Funiu Mountain.[Methods]The seed density,number of species and species composition of the soil seed bank of typical plant communities such as Themeda japonica,Imperata cylindrica,Vitex negundo,Quercus acutissima,Robinia pseudoacacia,Platycladus orientalis and Populus canadensis in the surface,top 0-5 and top 5-10 cm soil were studied.[Results]The seed reserves of plant communities at different succession stages were(220.00±95.39)–(2650.00±1064.52)seeds/m2.A total of 48 species were counted in the seed bank,belonging to 45 genera in 22 *** 27 species were identified in the above-ground vegetation,belonging to 25 genera in 14 *** the shallow soil where the seeds gathered,the seed densities of Imperata cylindrica and Themeda japonica were relatively *** reserves of plant communities with different naturalness degrees were(403.33±64.29)-(2110.00±356.79)seeds/m2.A total of 67 species were counted in the seed bank,belonging to 64 genera in 37 families.A total of 45 species were identified in the above-ground vegetation,belonging to 43 genera in 28 *** the soil layers of 0-5 and 5-10 cm,the seed density of natural secondary *** was higher than those of planted ***,*** and *** each soil layer,the species number of natural secondary *** forest was slightly smaller than those of planted ***,*** and *** forests.[Conclusions]The seeds in the soil seed bank may not completely come from existing above-ground *** assistance is required for vegetation restoration or reconstruction relying on soil seed bank,to ensure the direction of community succession.

关键词： Soil seed bank Seed density Species number Species composition Hilly area Funiu Mountain

cDNA-SCoT Analysis of Differential Expressed Genes in Allium tuberosum Induced by Botrytis cinerea

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Asian Agricultural Research 2020年第10期12卷 38-41页

作者： Minghui CHEN Zhilu ZHANG yanjiao LI Pengqiang YAO Shiping CHENG Ganqing zhao Key Laboratory of Ecological Restoration in Hilly Area Pingdingshan UniversityPingdingshang 467000China

[Objectives]To investigate the molecular mechanism of gene differential expression in *** under Botrytis cinerea stress.[Methods]The leaf of *** was inoculated with *** to induce resistance,then leaf samples were taken at 2,4,8,12 and 24 h,respectively,after inoculation,and used for cDNA-SCoT analysis to detect the gene differential expressions.[Results]More than 800 bands with length of 100-1800 bp were obtained using 60 SCoT primers.A total of 40 differentially expressed EST sequences were screened out,and 18 non-redundant ESTs with high quality were obtained by cluster analyses of the ESTs *** results of BlastN showed that the differential genes of *** mainly related to disease defense,signal transduction,and protein metabolism.[Conclusions]Further analysis of gene function indicated that brassinosteroid biosynthesis-like proteins,NBS-LRR type disease resistance protein,jasmonic acid induced protein,abscisic stress ripening protein may be involved in the process of the incompatible interaction between the *** and ***.

关键词： Allium tuberosum Grey mould cDNA-SCoT Differential genes