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中华器官移植杂志 2024年第2期45卷 96-103页

作者：李晓帆李芳谢智强许敏郑焱华何春晓李欣桐温雪梅李乃农福建医科大学附属协和医院血液科福建省血液病研究所福建省血液病学重点实验室福州350000 复旦大学附属中山医院厦门医院老年科厦门361015

目的探讨程序性死亡受体1单克隆抗体(PD-1单抗)加重allo-HSCT小鼠炎性损伤和慢性移植物抗宿主病(chronic graft versus host disease,cGVHD)的作用机制。方法将DBA/2小鼠的骨髓和脾细胞注射到白消安联合氟达拉滨化疗方案预处理BALB/C小... 详细信息

目的探讨程序性死亡受体1单克隆抗体(PD-1单抗)加重allo-HSCT小鼠炎性损伤和慢性移植物抗宿主病(chronic graft versus host disease,cGVHD)的作用机制。方法将DBA/2小鼠的骨髓和脾细胞注射到白消安联合氟达拉滨化疗方案预处理BALB/C小鼠构建cGVHD模型(n=48),将BALB/c(H-2Kd)小鼠按随机数字法分为炎性组(n=8)和对照组[注射磷酸盐缓冲液(PBS),n=8],使用酵母多糖诱导炎性组小鼠为炎症状态。观察记录两组小鼠移植后体重变化、生存情况以及cGVHD的表现,另取两组小鼠提取受累靶器官(皮肤、肝脏、小肠、结肠、肺脏)组织进行病理评分(每组n=4);体外实验取8~12周的BALB/C小鼠按上述方法建立炎症cGVHD模型,同样按照随机数字法分成PD-1组(注射PD-1单克隆抗体)和对照组(每组n=12),观察记录两组小鼠移植后体重变化、生存情况以及cGVHD的表现,另取小鼠应用流式细胞技术检测靶器官的细胞亚群、表面的共刺激分子等(n=4)。结果炎性组呈现更显著的cGVHD改变,小鼠死亡率高,体重下降快,GVHD的症状更严重。炎性组小鼠肝脏和肺脏的替代活化的巨噬细胞(M2)比例明显低于对照组(P<0.05),经典活化的巨噬细胞(M1)无差别。在体外实验中,炎性组相较对照组脾细胞M1比例均增多,M2比例明显均减少。分泌的白细胞介素(IL)-4,IL-10减少,共刺激分子CD80和CD86增多。结论PD-1单抗可加重allo-HSCT小鼠的炎性损伤和cGVHD,其作用机制可能与调节性T细胞(regulatory cell,Treg)减少相关。

关键词：造血干细胞移植炎症移植物抗宿主病调节性T细胞

Charge adaptive phytochemical-based nanoparticles for eradication of methicillin-resistant staphylococcus aureus biofilms

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Asian Journal of Pharmaceutical Sciences 2024年第3期19卷 160-176页

作者： Xilong Cui Fanhui Liu Shuang Cai Tingting Wang Sidi zheng Xinshu Zou Linlin Wang Siqi He yanhua Li Zhiyun Zhang College of Veterinary Medicine Northeast Agricultural UniversityHarbin 150030China Heilongjiang Key Laboratory for Animal Disease Control and Pharmaceutical Development Harbin 150030China

The intrinsic resistance of MRSA coupled with biofilm antibiotic tolerance challenges the antibiotic treatment of MRSA biofilm ***-based nanoplatform is a promising emerging approach for treatment of biofilm ***,their therapeutic efficacy was restricted by the low drug loading capacity and lack of ***,we constructed a surface charge adaptive phytochemical-based nanoparticle with high isoliquiritigenin(ISL)loading content for effective treatment of MRSA biofilm.A dimeric ISL prodrug(ISL-G2)bearing a lipase responsive ester bond was synthesized,and then encapsulated into the amphiphilic quaternized *** obtained ISL-G2loaded NPs possessed positively charged surface,which allowed cis-aconityl-D-tyrosine(CA-Tyr)binding via electrostatic interaction to obtain ISL-G2@TMDCOS-Tyr *** NPs maintained their negatively charged surface,thus prolonging the blood circulation *** response to low pH in the biofilms,the fast removal of CA-Tyr led to a shift in their surface charge from negative to positive,which enhanced the accumulation and penetration of NPs in the ***,the pH-triggered release of D-tyrosine dispersed the biofilm and lipase-triggered released of ISL effectively kill biofilm *** in vivo study was performed on a MRSA biofilm infected wound *** phytochemical-based system led to~2log CFU(>99%)reduction of biofilm MRSA as compared to untreated wound(P<0.001)with negligible biotoxicity in *** phytochemical dimer nanoplatform shows great potential for long-term treatment of resistant bacterial infections.

关键词： MRSA biofilm Isoliquiritigenin Dimer prodrug Charge adaptive Responsive nanoparticles

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网络信息资源及信息检索能力的培养

情报理论与实践 1998年第1期21卷 55-58页

作者：郑燕华上海交通大学情报所

网络信息资源及信息检索能力的培养●郑燕华（上海交通大学情报所上海２０００３０）ＡｂｓｔｒａｃｔＩｎｔｅｒｎｅｔｉｓｔｈｅｌａｒｇｅｓｔｉｎｆｏｒｍａｔｉｏｎｒｅｓｏｕｒｃｅｉｎｔｈｅｗｏｒｌｄ．Ｗｉｔｈｔｈｅｐｏｐｕ... 详细信息

网络信息资源及信息检索能力的培养●郑燕华（上海交通大学情报所上海２０００３０）ＡｂｓｔｒａｃｔＩｎｔｅｒｎｅｔｉｓｔｈｅｌａｒｇｅｓｔｉｎｆｏｒｍａｔｉｏｎｒｅｓｏｕｒｃｅｉｎｔｈｅｗｏｒｌｄ．Ｗｉｔｈｔｈｅｐｏｐｕｌａｒｉｚａｔｉｏｎｏｆｉｎｆｏｒ...

关键词：网络信息资源情报检索能力培养信息资源

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光盘数据库系统的信息检索特性分析

情报理论与实践 1998年第4期21卷 236-238,207页

作者：郑燕华上海交通大学情报所

光盘数据库系统的信息检索特性分析郑燕华（上海交通大学情报所上海２０００３０ＡｂｓｔｒａｃｔＴｈｒｅｅｋｉｎｄｓｏｆｃｏｍｍｏｎｌｙ－ｕｓｅｄＣＤ－ＲＯＭｓｙｓｔｅｍｓａｒｅｉｎｔｒｏｄｕｃｅｄ．Ｔｈｅｆｕｎｃｔｉｏｎ... 详细信息

光盘数据库系统的信息检索特性分析郑燕华（上海交通大学情报所上海２０００３０ＡｂｓｔｒａｃｔＴｈｒｅｅｋｉｎｄｓｏｆｃｏｍｍｏｎｌｙ－ｕｓｅｄＣＤ－ＲＯＭｓｙｓｔｅｍｓａｒｅｉｎｔｒｏｄｕｃｅｄ．Ｔｈｅｆｕｎｃｔｉｏｎｓｏｆｔｈｅｓｅｓｏｆｔｗａｒｅｓ...

关键词：光盘数据库系统信息检索检索指令语言

Solvothermal synthesis of nanocrystalline FeS_2

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Science China(Physics,Mechanics & Astronomy) 2005年第2期48卷 188-200页

作者： CHEN yanhua1, zheng Yufeng1, ZHANG Xiaogang2, SUN Yanfei1 & DONG Youzhong11. Physics Department of Xinjiang University, Urumqi 830046, China 2. Institute of Applied Chemistry of Xinjiang University, Urumqi 830046, China Physics Department of Xinjiang University Urumqi China Institute of Applied Chemistry of Xinjiang University Urumqi China

This paper reports on the synthesis of the nanocrystalline FeS2 via a solvent-thermal process. Using FeSO4 and NH2CSNH2 as precursors and polyvinyl- pyrrolidone (PVP) as a protective agent (dispersant), we have successfully synthesized nanocrystalline FeS2 both under acidic conditions (pH = 5) and under alkali conditions (pH = 10) at various temperatures and for different time in alcohol-water solution. X-ray diffraction (XRD), Transmission electron microscopy (TEM) and Ultraviolet Visible (UV-VIS) spectroscopy were used to characterize the morphology, structure, particle size and optical characterization of sample synthesized under acidic condition, and the XRD pattern was refined by Rietveld method. The experimental result shows that nanocrystalline FeS2 with uniform size, highly crystallized quality and excellent dispersivity has been synthesized at 200℃for 36 h.

关键词： FeS2 (Pyrite), solvothermal, PVP, the Rietveld method.

“中西融合、海陆互通”人才培养模式创新与实践——以浙江国际海运职业技术学院国际邮轮乘务管理专业为例

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职业技术教育 2020年第32期41卷 18-23页

作者：郑燕华浙江国际海运职业技术学院舟山316021

邮轮旅游业是现代服务业的重要组成部分,邮轮人才培养的关键是人才培养模式的创新。浙江国际航运职业技术学院国际邮轮乘务管理专业创新地构建了“中西融合、海陆互通”人才培养模式,即面向国际邮轮公司和国内外品牌酒店两个就业岗位目... 详细信息

邮轮旅游业是现代服务业的重要组成部分,邮轮人才培养的关键是人才培养模式的创新。浙江国际航运职业技术学院国际邮轮乘务管理专业创新地构建了“中西融合、海陆互通”人才培养模式,即面向国际邮轮公司和国内外品牌酒店两个就业岗位目标,对接行业标准,按专业基础、专业核心、专业方向和专业实践四个阶段设置相应的课程,构建“三三制”实践教学体系,高标准建设校内外实训基地,将融合中西特色的技能课程贯穿于人才培养全过程,突出学生的职业技能、职业素养和综合能力培养。该模式取得一定成效,但在人才培养反馈与评估、校企合作深度与广度、学生人文精神培养方面还存在不足。

关键词：中西融合海陆互通人才培养模式校企合作国际邮轮乘务管理专业

Micelle-directed self-assembly of single-crystal-like mesoporous stoichiometric oxides for high-performance lithium storage

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National Science Review 2024年第4期11卷 195-205页

作者： yanhua Wan Changyao Wang Xingmiao Zhang Yang Yin Mengmeng Liu Bing Ma Linlin Duan Yuzhu Ma Wei Zhang Changlin zheng Dongliang Chao Fei Wang Yongyao Xia Wei Li Department of Chemistry Laboratory of Advanced Materials Shanghai Key Laboratory of Molecular Catalysis and Innovative Materials State Key Laboratory of Molecular Engineering of Polymers College of Chemistry and Materials ScienceFudan University State Key Laboratory of Surface Physics and Department of Physics Fudan University

Due to their uncontrollable assembly and crystallization process,the synthesis of mesoporous metal oxide single crystals remains a formidable ***,we report the synthesis of single-crystal-like mesoporous Li2TiSiO5by using soft micelles as *** key lies in the atomic-scale self-assembly and step-crystallization processes,which ensure the formation of single-crystal-like mesoporous Li2TiSiO5microparticles via an oriented attachment growth mechanism under the confinement of an in-situ formed carbon *** mesoporous Li2TiSiO5anode achieves a superior rate capability(148 mAh g-1at5.0 A g-1) and outstanding long-term cycling stability(138 mAh g-1after 3000 cycles at 2.0 A g-1) for lithium storage as a result of the ultrafast Li+diffusion caused by penetrating mesochannels and nanosized crystal frameworks（5-10 nm）.In comparison,bulk Li2TiSiO5exhibits poor rate capability and cycle performance due to micron-scale diffusion *** method is very simple and reproducible,heralding a new way of designing and synthesizing mesoporous single crystals with controllable frameworks and chemical functionalities.

关键词： mesoporous materials micelles single-crystal-like Li2TiSiO5 lithium-ion batteries

TLR2单克隆抗体对大鼠角膜移植术后植片存活的保护作用

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中华实验眼科杂志 2015年第10期33卷 887-891页

作者：白浪郑艳华梁伟怡南方医科大学南方医院眼科广州510515

背景 Toll样受体2（TLR2）在移植免疫相关疾病中的作用越来越受到关注,阻断TLR2对心脏和肾脏移植物的保护作用已有研究报道,但TLR2是否具有抑制角膜移植排斥反应的作用尚未证实.目的观察TLR2单克隆抗体对同种异体大鼠角膜移植术后植片... 详细信息

背景 Toll样受体2（TLR2）在移植免疫相关疾病中的作用越来越受到关注,阻断TLR2对心脏和肾脏移植物的保护作用已有研究报道,但TLR2是否具有抑制角膜移植排斥反应的作用尚未证实.目的观察TLR2单克隆抗体对同种异体大鼠角膜移植术后植片存活情况的影响.方法取24只SPF级雌性Wistar大鼠作为受体,12只SPF级SD大鼠作为供体,体质量均为180～220 g.采用同种异体角膜移植的方法在Wistar大鼠右眼实施穿透角膜移植术,制备大鼠角膜移植模型.采用随机数字表法将模型眼分为单纯模型组和TLR2单克隆抗体组,TLR2单克隆抗体组分别于术后0、2、4、6、8d球结膜下注射TLR2单克隆抗体15 μg/30μl,单纯模型组大鼠同法注射等容量生理盐水.术后每日于裂隙灯显微镜下观察各组大鼠角膜植片水肿程度、透明度和新生血管形成情况,参照Holland等的评分标准对排斥反应指数（RI）进行评分;分别于术后第9天、第15天收集各组3只大鼠角膜组织,另取3只正常Wistar大鼠眼球作为正常对照,行常规组织病理学检查.各组取6只大鼠用于生存分析观察.结果术后1～4 d,裂隙灯显微镜下2个组角膜均轻度水肿,术后9～14d,单纯模型组大鼠角膜植片布满新生血管,可见植片水肿、混浊,而TLR2单克隆抗体组大鼠植片至术后第15天开始混浊.术后5、9、15d,单纯模型组大鼠RI评分均明显高于TLR2单克隆抗体组,差异均有统计学意义（t=4.183、4.954、13.506,均P＜0.05）;TLR2单克隆抗体组角膜植存活时间为15.5d,95％可信区间（CI）为14.9～16.1,单纯模型组角膜植片存活时间为9.5d,95％ CI为8.7～10.3,2个组间差异有统计学意义（Z=12.728,P=0.001）.角膜组织病理学检查显示,单纯模型组大鼠角膜基质层水肿明显,角膜组织中可见大量炎性细胞浸润和新生血管管腔形成,而在TLR2单克隆抗体组中仅见少量炎性细胞和新生血管.结论 TLR2单克隆抗体能减轻角膜移植术后的炎症反应,延长角膜植片的存活时间.

关键词： Toll样受体2 单克隆抗体/用法和用量穿透角膜移植/病理植片排斥/病理植片存活 /药物作用动物模型

Development and validation of a nomogram prediction model for the risk of parastomal hernia

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Intelligent Medicine 2024年第2期4卷 128-133页

作者： Huasheng Liu Weiqin Wang Chen Qin Hongxia Wang Wei Qi yanhua Wei Longbo zheng Jilin Hu Department of Gastroenterology Affiliated Hospital of Qingdao UniversityQingdaoShandong 266003China Qingdao Municipal Hospital Group QingdaoShandong 266300China Qingdao Center Medical Group QingdaoShandong 266042China

Background Parastomal hernia is one of the potential complications after *** is currently no early risk assessment tool for parastomal *** The current investigation was conducted using retrospective studies.A total of 302 cases were used develop and internally to validate a nomogram prediction model,and 67 cases were used for external *** risk factors for parastomal hernia after permanent sigmoid colostomy were assessed via univariate analysis and binary logistic regression *** nomogram prediction model was established based on independent risk *** Body mass index,serum albumin,age,sex,and stoma diameter were independent risk factors for parastomal *** areas under the receiver operating characteristic curves were 0.909 in the development group and 0.801 in the validation *** Hosmer-Lemeshow test(P>0.05)and calibration curves indicated good consistency between actual observations and predicted *** A nomogram prediction model was constructed and validated based on risk factors for parastomal *** nomogram could be generalized to patients undergoing surgery for stoma by specialized surgeons to provide relevant references for stoma patients.

关键词： Nomogram Parastomal hernia Risk factors