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C13C4.5/Spinster, an evolutionarily conserved protein that regulates fertility in C. elegans through a lysosome-mediated lipid metabolism process

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Protein & Cell 2013年第5期4卷 364-372页

作者： Mei Han Hao Chang Peng Zhang tao Chen yanhua Zhao Yongdeng Zhang Pingsheng Liu tao Xu Pingyong Xu National Laboratory of Biomacromolecules Institute of BiophysicsChinese Academy of SciencesBeijing 100101China University of Chinese Academy of Sciences Beijing 100049China School of Life Sciences University of Science and Technology of ChinaHefei 230027China College of Engineering and Biodynamic Optical Imaging Center(BIOPIC)Peking UniversityBeijing 100871China Key Laboratory of Interdisciplinary Research Institute of BiophysicsChinese Academy of SciencesBeijing 100101China

Lipid droplets, which are conserved across almost all species, are cytoplasmic organelles used to store neutral lipids. Identification of lipid droplet regulators will be conducive to resolving obesity and other fat-associated diseases. In this paper, we selected 11 candidates that might be associated with lipid metabolism in Caenorhabditis elegans . Using a BODIPY 493/503-based flow cytometry screen, 6 negative and 3 positive regulators of fat content were identified. We selected one negative regulator of lipid content, C13C4.5 , for future study. C13C4.5 was mainly expressed in the worm intestine. We found that this gene was important for maintaining the metabolism of lipid droplets. Biochemical results revealed that 50% of triacylglycerol (TAG) was lost in C13C4.5 knockout worms. Stimulated Raman scattering (SRS) signals in C13C4.5 mutants showed only 49.6% of the fat content in the proximal intestinal region and 86.3% in the distal intestinal region compared with wild type animals. The mean values of lipid droplet size and intensity in C13C4.5 knockout animals were found to be significantly decreased compared with those in wild type worms. The LMP-1-labeled membrane structures in worm intestines were also enlarged in C13C4.5 mutant animals. Finally, fertility defects were found in C13C4.5 ( ok2087 ) mutants. Taken together, these results indicate that C13C4.5 may regulate the fertility of C. elegans by changing the size and fat content of lipid droplets by interfering with lysosomal morphology and function.

关键词： C13C4.5 Spinster lipid droplet SRS mi-croscopy lysosomal enlargement fertility

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针药结合对急性缺血性脑卒中未溶栓患者疗效观察

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首都食品与医药 2023年第5期30卷 148-150页

作者：王传兰于建锋季艳张丽彭飞吴炜朱敏陶燕华庄向东黄春裕江苏省南通市通州区中医院江苏南通226300

目的观察针药结合对急性缺血性脑卒中未溶栓患者的临床疗效。方法将2021年10月-2022年6月期间,于江苏省南通市通州区中医院住院的急性缺血性脑卒中的患者60例,随机分为两组,每组30例。对照组给予常规基础治疗加依达拉奉右莰醇30mg静滴q... 详细信息

目的观察针药结合对急性缺血性脑卒中未溶栓患者的临床疗效。方法将2021年10月-2022年6月期间,于江苏省南通市通州区中医院住院的急性缺血性脑卒中的患者60例,随机分为两组,每组30例。对照组给予常规基础治疗加依达拉奉右莰醇30mg静滴q12h。治疗组在相同西药治疗的基础上,加用头皮针治疗。2周后,分析治疗效果,以及患者治疗前MRS值对比。结果两组患者均有比较好的治疗效果,针药联合治疗组的总有效率为93.3%,高于仅用西药治疗的对照组(83.3%),两组之间的疗效有显著差异(P0.05),存在可比性。结论针药结合治疗急性缺血性脑卒中未溶栓患者临床疗效更优,同时头颅磁共振波谱变化治疗前存在可比性,为证实针药结合治疗组优于对照组进一步奠定基础。

关键词：头皮针依达拉奉右莰醇磁共振波谱急性缺血性脑卒中中风

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Modeling diurnal variation of ground thermal radiance images using energy balance model and endmember composing technique

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Science China(Technological Sciences) 2012年第11期55卷 3223-3231页

作者： ZHAO LiMin GU XingFa YU tao WAN Wei XIONG Pan XIE yanhua ZHANG Lun State Key Laboratory of Remote Sensing Science Jointly Sponsored by the Institute of Remote Sensing Applications of Chinese Academy of Sciences and Beijing Normal UniversityBeijing 100101China The Center for National Spaceborne Demonstration Beijing 100101China School of Earth and Space Sciences Peking UniversityBeijing 100871China Institute of Earthquake Science China Earthquake AdministrationBeijing 100036China

Modeling and analyzing dynamic changes of land thermal radiance scenes play an important role in thermal remote sensing. In this paper, the diurnal variation of ground surface thermal scene is mainly discussed. Firstly, based on the land surface energy balance equation, the diurnal variation of land surface temperatures （LSTs） over bare land covers were simulated by an analyt- ical thermal model with second harmonic terms, and the diurnal LST variation of vegetation canopy was simulated using the Cupid model. Secondly, normalized difference vegetation index （NDVI）, normalized difference water index （NDWI）, and ratio resident-area index （RRI） were used to evaluate the endmember abundance of four land cover types including vegetation, bare soil, impervious and water area, which were calculated from IKONOS visible and near infrared （VNIR） bands. Finally, the thermal radiance scenes at various times and view angles were modeled based on the linear-energy-mixing hypothesis. The re- suits showed that the simulated daily LST variations for vegetated and bare surfaces are correlated with the measured values with a maximum standard deviation of 2.7℃, that land thermal radiant textures with high-resolution are restored from the lin- ear-energy-mixing method, and that the information abundance of the scene are related to the distribution of land cover, the imaging time, and the view angle.

关键词： surface energy balance model surface temperature endmember index thermal radiance scene modeling high-spatialresolution

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Targeted disruption of tyrosinase causes melanin reduction in Carassius auratus cuvieri and its hybrid progeny

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Science China(Life Sciences) 2019年第9期62卷 1194-1202页

作者： Qingfeng Liu yanhua Qi Qiuli Liang Jia Song Junmei Liu Wuhui Li Yuqin Shu Min tao Chun Zhang Qinbo Qin Jing Wang Shaojun Liu State Key Laboratory of Developmental Biology of Freshwater Fish Hunan Normal UniversityChangsha 410081China College of Life Sciences Hunan Normal UniversityChangsha 410081China

The white crucian carp(Carassius auratus cuvieri,WCC) not only is one of the most economically important fish in Asia,characterized by strong reproductive ability and rapid growth rates,but also represents a good germplasm to produce hybrid progenies with *** knockout technique provides a safe and acceptant way for fish *** gene knockout in WCC and its hybrid progeny will be of great importance for both genetic studies and hybridization ***(TYR) is a key enzyme in melanin *** of tyr in zebrafish and mice results in mosaic pigmentation or total ***,we successfully used CRISPR-Cas9 to target tyr in WCC and its hybrid progeny(WR) derived from the cross of WCC(♀) and red crucian carp(Carassius auratus red var.,RCC,♂).The level of TYR protein was significantly reduced in mutant *** the mutant WCC and the mutant WR showed different degrees of melanin reduction compared with the wild-type sibling control fish,resulting from different mutation efficiency ranging from 60% to 90%.In addition,the transcriptional expression profiles of a series of pivotal pigment synthesis genes,***1,mitfa,mitfb,dct and sox10,were down-regulated in tyr-CRISPR WCC,which ultimately caused a reduction in melanin *** results demonstrated that tyr plays a key role in melanin synthesis in WCC and WR,and CRISPR-Cas9 is an effective tool for modifying the genome of economical ***,the tyr-CRISPR models could be valuable in understanding fundamental mechanisms of pigment formation in non-model fish.

关键词： white crucian carp hybridization CRISPR-Cas9 tyrosinase pigmentation

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Genome-Wide Identification, Evolution and Expression Analyses of GA2ox Gene Family in Brassica napus L.

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Phyton-International Journal of Experimental Botany 2023年第3期92卷 815-835页

作者： yanhua Li Hualei Huang Youming Shi Shuqin Huang tao Liu Changming Xiao Xiaoqing Tian Ping Zhao Xiaoyan Dai taocui Huang Yan Zhou Chongqing Academy of Agricultural Sciences Chongqing401329China Chongqing Municipal Agricultural School Chongqing401329China Fengdu Agricultural Technology Service Center Chongqing408201China

Gibberellin 2-oxidases(GA2ox)are important enzymes that maintain the balance of bioactive GAs in ***2ox genes have been identified and characterized in many plants,but these genes were not investigated in Brassica ***,we identified 31 GA2ox genes in *** and 15 of these BnaGA2ox genes were distributed in the A and C *** localization predictions suggested that all BnaGA2ox proteins were localized in the cytoplasm,and gene structure analysis showed that the BnaGA2ox genes contained 2–4 *** analysis indicated that BnGA2ox family proteins in monocotyledons and dicotyledons can be divided into four groups,including two C_(19)-GA2ox and two C_(20)-GA2ox *** 4 is a C_(20)-GA2ox Class discovered *** BnaGA2ox genes had a syntenic relationship with AtGA2ox ***2ox genes in the C subgenome had experienced stronger selection pressure than genes in the A ***2ox genes were highly expressed in specific tissues such as those involved in growth and development,and most of them were mainly involved in abiotic responses,regulation of phytohormones and growth and *** study provided a valuable evolutionary analysis of GA2ox genes in monocotyledons and dicotyledons,as well as an insight into the biological functions of GA2ox family genes in ***.

关键词： Brassica napus GA2ox gene family evolution expression patterns

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Efficacy of Cigu Xiaozhi pill(慈菇消脂丸) on non-alcoholic steatohepatitis-associated lipoapoptosis through stress-activated c-Jun N-terminal kinase signalling pathway

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Journal of Traditional Chinese Medicine 2021年第1期41卷 79-88页

作者： MA yanhua YU Chengzu WU Yan HAN tao YANG Shaojun SHI Xia College of Clinical Medicine Gansu University of Chinese MedicineLanzhou 730000China College of Pharmacy Gansu University of Chinese MedicineLanzhou 730000China Guangxi Beihai Hospital of Traditional Chinese Medicine Beihai 536000China Center of Teaching experiment Gansu University of Chinese MedicineLanzhou 730000China

OBJECTIVE: To investigate the efficacy of Cigu Xiaozhi pill(慈菇消脂丸, CGXZ) on non-alcoholic steatohepatitis(NASH)-associated lipoapoptosis through the stress-activated c-Jun N-terminal kinase(JNK)/stress-activated protein kinase signalling ***: Sixty male Sprague-Dawley rats were randomly divided into the following groups(10 rats each): blank control, model, low-dose CGXZ,medium-dose CGXZ, high-dose CGXZ, and positive control(treated with SP600125, a JNK inhibitor).The NASH model was established and the histomor-phological characteristics of haematoxylin and eosin-stained liver tissues were examined under a light microscope. Cell apoptosis in liver tissues was assessed via terminal deoxynucleotidyl transferase d UTP nick-end labelling assay. In addition, the m RNA and protein expression levels of p-JNK,p-c-Jun, caspase-8, Fas, and Fas-L were determined via fluorescence-based quantitative real-time PCR,immunohistochemical and Western blot ***: Histopathological examination of the liver showed that the model rats had moderate-to-severe steatosis with infiltration of inflammatory cells as well as significantly higher expression levels of the p-JNK, p-c-Jun, caspase-8, Fas, and Fas-L proteins, compared with those in the blank control group(P < 0.01). Hepatic lobules of the rats in the treatment groups showed significantly reduced vacuolar degeneration and steatosis as well as alleviated inflammatory cell infiltration. The high and medium-dose CGXZ groups exhibited significantly lower m RNA and protein expression levels of p-JNK, p-c-Jun, caspase-8, Fas, and Fas-L, compared with those in the model group(P < 0.05 or P <0.01).CONCLUSION: CGXZ pill inhibited the onset of hepatocyte apoptosis by regulating the expression of p-JNK, p-c-Jun, caspase-8, Fas, and Fas-L, thereby exerting therapeutic effects against NASH.

关键词： Non-alcoholic fatty liver disease JNK mitogen-activated protein kinases apoptosis stress-activated signaling pathway Cigu Xiaozhi pill

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Epsilon-near-zero photonics:infinite potentials

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Photonics Research 2021年第8期9卷 1616-1644页

作者： JIAYE WU ZE tao XIE yanhua SHA H.Y.FU QIAN LI School of Electronic and Computer Engineering Peking UniversityShenzhen 518055China Tsinghua Shenzhen International Graduate School Tsinghua UniversityShenzhen 518055China

With its unique and exclusive linear and nonlinear optical characteristics,epsilon-near-zero(ENZ)photonics has drawn a tremendous amount of attention in the recent decade in the fields of nanophotonics,nonlinear optics,plasmonics,light-matter interactions,material science,applied optical science,*** extraordinary optical properties,relatively high tuning flexibility,and CMOS compatibility of ENZ materials make them popular and competitive candidates for nanophotonic devices and on-chip integration in all-optical and electro-optical *** exclusive features and high performance,ENZ photonics can play a big role in optical communications and optical data *** this review,we give a focused discussion on recent advances of the theoretical and experimental studies on ENZ photonics,especially in the regime of nonlinear ENZ nanophotonics and its ***,we overview the basics of the ENZ concepts,mechanisms,and nonlinear ENZ *** the new advancements in theoretical and experimental optical physics are *** nanophotonic applications,the recent decades saw rapid developments in various kinds of different ENZ-based devices and systems,which are discussed and analyzed in ***,we give our perspectives on where future endeavors can be made.

关键词： tuning nonlinear photonics

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Operation of the CSNS Penning surface H^- ion source

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Science China(Physics,Mechanics & Astronomy) 2011年第S2期54卷 245-248页

作者： WU XiaoBing OUYANG HuaFu CHI YunLong HE Wei HUANG tao LI Gang LIU YingMan LU yanhua XU taoGuang ZHANG JunSong ZHANG HuaShun ZHAO FuXiang Institute of High Energy Physics Chinese Academy of SciencesBeijing 100049China Graduate University of Chinese Academy of Sciences Beijing 100049China

The accelerator complex of the China Spallation Neutron Source (CSNS) consists of a H- linear accelerator (linac) and a rapid cycling synchrotron (RCS). The linac contains a Penning surface H- ion source. The designed energy and the beam current of the source are 50 keV and 20 mA respectively, with a normalized root mean square (norm. rms.) emittance of 0.2π mm mrad. The manufactures and tests of the discharge chamber are in great progress. The construction of H- ion source test stand has been completed, and the operation of the source is also in progress. Stable H- ion beams with energy of 50 keV and current up to 50 mA are attained. Emittance measurement for the H- beam is being prepared.

关键词： CSNS Penning H- ion source discharge chamber current emittance

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Dynamic mechanism and present situation of rural settlement evolution in China

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Journal of Geographical Sciences 2013年第3期23卷 513-524页

作者： ZHOU Guohua HE yanhua TANG Chengli YU tao XIAO Guozhen ZHONG Ting College of Resources and Environment Science Hunan Normal University Changsha 410081 China Science Press Beijing 100717 China

This paper provides a detailed analysis of the factors influencing the evolution of rural settlements, including natural environmental constraints, infrastructure, regional cultural inheritance and integration, urbanization and rural industrial transformation, land use refor- mation and innovation, rural household behavior conversion, macro-control policy factors, and so on. Based on differences between the ways and degree of effect on rural settlement evolution, these factors are classified into basic factors, new-type factors and mutation factors The drive of basic factors mainly focuses on the traditional inheritance of rural settlements, the new-type factors mainly affect rural settlement transition, and the mutation factors may bring about sudden changes. All these factors constitute a ＂three-wheel＂ driving mechanism for the evolution of rural settlements, and shape three typical driver paths： slow smooth path under the basic factors, new path to rapid development under the new-type factors, and the sudden change path under the mutation factors. The paper also investigates the overall situation of rural settlement evolution in the aspects of settlement system, settlement scale, settlement morphology, settlement function, settlement culture, settlement environment, etc. The general process of rural settlement evolution is divided into four stages： initial, transi- tional, developmental, and mature stages.

关键词： rural settlements evolution evolution stage dynamic mechanism China

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The chimeric genes in the hybrid lineage of Carassius auratus cuvieri(♀)×Carassius auratus red var.(♂)

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Science China(Life Sciences) 2018年第9期61卷 1079-1089页

作者： Qingfeng Liu yanhua Qi Qiuli Liang Xiujuan Xu Fangzhou Hu Jing Wang Jun Xiao Shi Wang Wuhui Li Min tao Qinbo Qin Rurong Zhao Zhanzhou Yao Shaojun Liu State Key Laboratory of Developmental Biology of Freshwater Fish Hunan Normal University College of Life Sciences Hunan Normal University

Hybridization can combine the genomes of different strains or species, which leads to changes of genotype and phenotype in the hybrids. In this study, we aimed to investigate the genetic variations of hybrids(WR-F1 and WR-F2) derived from the intraspecific hybridization of white crucian carp(Carassius auratus cuvieri, WCC, ♀) and red crucian carp(Carassius auratus red var., RCC, ♂). Here, we compared the orthologous genes in the liver transcriptomes of hybrids with those of WCC and RCC, and classified the orthologous genes into eight gene patterns within three categories(chimera, mutant, and biparental origin genes).The results revealed 19.04%, 4.17% chimeric genes and 6.90%, 5.05% mutations of orthologous genes in WR-F1 and WR-F2 respectively. Seventeen of twenty-three characterized genes(77%) were confirmed to be the chimeras at the genomic DNA *** GO classification discovered that some chimeric and mutant genes were related to metabolic process, immune system and developmental process in WR-F1. Our results provide the new evidence that hybridization can combine the parental genomes,leading to changes in the genotype of the resultant hybrids. This is the first report on the formation of chimeric genes from fish intraspecific hybridization, which is potentially interesting from the context of both evolution and the genetic breeding of fish.

关键词： intraspecific hybridization chimeric genes transcriptomes sequence validation genetic variation

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