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Acta Oceanologica Sinica 2006年第6期25卷 145-152页

作者： hou yanhua LI Fuchao QIN Song WANG Quanfu Institute of Oceanology Chinese Academy of Sciences Qingdao 266071 China Graduate School Chinese Academy of Sciences Beijing 100039 China School of Ocean Harbin Institute of Technology Weihai 264209 China

Optimal conditions for protoplasts formation of marine Actinomycete sp. isolate M048 were described, dense and disperse mycelia were cultured in SGGP medium, 0.5% glycine, lysozyme exposure （2 mg/cm^3, 37 ℃, 40 min）, and the concentration of sucrose in protoplast buffer was 0.4 mol/dm^3 for keeping the balance of osmotic pressure. Using PEG-mediated pmtoplasts transformarion, the transformation frequency was 89 transformants per microgramme of pIJ702. Meanwhile, an effective transformation procedure was established based on intergeneric conjugation from E. coli ET12567 （pUZS002） using shuttle vectors pPM801, pPM803 and a φC31-derived integration vector pIJ8600 containing onT and attP fragments. Transformation frequencies were 5.30 ×10^-4 ±0.26 ×10^-4 , 8.92 ×10^-4 ±0. 19 ×10^-4 and 6.38 ×10^-5 ±0.41×10^-5 respectively. Further, the heterologous expression of the allophycocyanin gene （apc） in the strain M048 was used to demonstrate this transformation system. SDS - PAGE and Western blot analysis confirmed the expression of recombinant APC （rAPC）.

关键词： allophycocyanin protoplast intergeneric conjugantion exconjugant marine Actinomycetes

Effect of Tea Polyphenols on Lipid Oxidation of Nemipterus VirgatusSurimi Cake During Ice Storage

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Journal of Harbin Institute of Technology(New Series) 2021年第3期28卷 9-16页

作者： Quanfu Wang Yifan Wang yanhua hou Yatong Wang Yujin Li Xuepeng Li School of Environment Harbin Institute of TechnologyHarbin 150090China School of Marine Science and Technology Harbin Institute of Technology(Weihai)Weihai 264209ShandongChina Shandong Provincial Engineering Technology Research Center of Marine Health Food Rongcheng 264300ShandongChina College of Food Science and Technology Bohai UniversityJinzhou 121013LiaoningChina

Surimi cake is a high value aquatic product. In this work, lipid oxidation and volatile compounds in Nemipterus virgatus surimi cake were investigated to evaluate the effectiveness of tea polyphenols in preventing or reducing lipid oxidation. Surimi cake was prepared in the presence or absence of 0.2% tea polyphenols, and was stored at 4 ℃ for 16 days. It was found that the peroxide value(POV) and thiobarbituric acid reactive substances(TBARS) values of the experimental groups(with 0.2% tea polyphenols) were much lower than those of the untreated surimi cake(p<0.05). In total, 52 compounds were presented in the volatiles of the surimi cake, such as ethanol, hexanal,(E,E)-2,4-decadienal, 2,3-octanedione, 2-hydroxy-propanamide, and 2,5-dimethyl-pyrazine. It indicated that tea polyphenols could reduce the amount of hexanal, ethanol, and(E,E)-2,4-decadienal, and delay the lipid oxidation of the surimi cake. Effects of lipid oxidation of the tea polyphenols on surimi cake were reduced during storage in ice.

关键词： surimi cake lipid oxidation tea polyphenols POV TBARS

Constructions of ROS-responsive titanium-hydroxyapatite implant for mesenchymal stem cell recruitment in peri-implant space and bone formation in osteoporosis microenvironment

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Bioactive Materials 2022年第12期7卷 56-71页

作者： Maohua Chen Yuting Sun yanhua hou Zhong Luo Menghuan Li Yujia Wei Maowen Chen Lu Tan Kaiyong Cai Yan Hu Key Laboratory of Biorheological Science and Technology Ministry of EducationCollege of BioengineeringChongqing UniversityChongqing400044China School of Life Science Chongqing UniversityChongqing400044China Chongqing Engineering Research Center of Pharmaceutical Science Chongqing Medical and Pharmaceutical CollegeChongqing401331China

To solve the issue of unsatisfactory recruitment of mesenchymal stem cells(MSCs)around implant in osteoporotic fractures,we fabricated a ROS-responsive system on titanium surface through hydroxyapatite coating and biomolecule *** porous hydroxyapatite and phosphorylated osteogenic growth peptides(p-OGP)were introduced onto titanium surface to synergistically improve osteogenic differentiation of *** the p-OGPpromoted expression of osteogenic related proteins,the calcium and phosphate ions were released through the degradation of hydroxyapatite and integrated into bone tissues to boost the mineralization of bone *** ROS-triggered release of DNA aptamer(Apt)19S in the osteoporotic microenvironment guides MSC migration to implant site due to its high affinity with alkaline phosphatase on the membrane of *** MSCs reached the implant interface,their osteogenic differentiation potential was enhanced by p-OGP and hydroxyapatite to promote bone *** study here provided a simple and novel strategy to prepare functional titanium implants for osteoporotic bone fracture repair.

关键词： Mesenchymal stem cell recruitment Osteogenic differentiation Hydroxyapatite Titanium Osteoporosis

Purification and Identification of Antioxidant Peptides from the Scallop Skirts of Mimachlamys Nobilis

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Journal of Harbin Institute of Technology(New Series) 2017年第5期24卷 46-52页

作者： Quanfu Wang Yifan Wang yanhua hou Yifeng Xu Meihong Yan Haoyuan Li Yuanping Liu Yujin Li School of Marine Science and Technology Harbin Institute of Technology Shandong Provincial Engineering Technology Research Center of Marine Health Food National and Local United Engineering Laboratory of Marine Functional Food Development (Shandong)

The processes of scallop meats can produce a large amount of by-products like scallop skirts,which are rich in nutrients. This work aimed to obtain the information about antioxidant peptides derived from scallop skirts of Mimachlamys nobilis. The hydro-lysates obtained by trypsin exhibited the highest antioxidant activity( 75.6%,79.5% and 76.7% using DPPH,hydroxyl and superoxide anion radical scavenging activity assays,respectively). The antioxidant peptides( MNAP) were separated and purified using UF membranes,GFC processes and RP-HPLC,and the antioxidant activity of MNAP was identified to be 85.7% using DPPH radical scavenging activity assay. The peptides( MNAP) were composed of two peptides with their molecular weights2 855.6 Da and 3 007.2 Da using MALDI-TOF-MS,respectively. The results suggested that the scallop skirts of Mimachlamys nobilis could be explored as a source of antioxidant peptides.

关键词： Mimachlamys nobilis antioxidant peptides purification scallop skirts hydro-lysates

不同麻醉方法对食管癌根治术患者术后睡眠质量的影响

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中华麻醉学杂志 2020年第4期40卷 500-501页

作者：侯艳华张加强武江霞郑州大学人民医院麻醉科 450003

术后睡眠障碍指术后患者睡眠和觉醒节律性交替紊乱,全麻因素可能与术后睡眠障碍有关。丙泊酚和七氟烷是临床常用麻醉药,右美托咪定是常用的麻醉辅助性用药。丙泊酚主要作用于腹外侧视前核核团的非睡眠促进神经元,是无痛胃镜术后睡眠障... 详细信息

术后睡眠障碍指术后患者睡眠和觉醒节律性交替紊乱,全麻因素可能与术后睡眠障碍有关。丙泊酚和七氟烷是临床常用麻醉药,右美托咪定是常用的麻醉辅助性用药。丙泊酚主要作用于腹外侧视前核核团的非睡眠促进神经元,是无痛胃镜术后睡眠障碍的独立危险因素;吸入麻醉药可促进该核团睡眠促进神经元c-Fos表达;右美托咪定可增加总睡眠时间并提高睡眠效率。

关键词：右美托咪定吸入麻醉药麻醉辅助七氟烷无痛胃镜睡眠效率觉醒节律睡眠时间

辽宁省农作物良种推广补贴政策实施情况与成效分析

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中国农业信息 2006年第9期 16-17页

作者：侯艳华辽宁省农村经济委员会

为加速粮食作物优良品种推广应用速度,扩大优良品种覆盖率,推进大豆产业振兴,确保国家粮食安全,2002年,国家开始实施主要粮食作物良种推广补贴项目.

关键词：辽宁省高油大豆优质水稻作物良种推广补贴良种补贴政策专用玉米成效分析

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铁岭市农业综合生产能力情况的调研报告

中国农业信息 2005年第7期 12-13页

作者：侯艳华辽宁省农村经济委员会种植业管理处

2005年,中央1号文件指出,切实加强农业综合生产能力建设,是农业和农村工作的主题.提高农业综合生产能力是党的十五大以来,中央一贯的农业发展战略.2004年,党中央国务院采取了一系列更直接更有力的政策措施,保护和调动了农民的生产积极性... 详细信息

2005年,中央1号文件指出,切实加强农业综合生产能力建设,是农业和农村工作的主题.提高农业综合生产能力是党的十五大以来,中央一贯的农业发展战略.2004年,党中央国务院采取了一系列更直接更有力的政策措施,保护和调动了农民的生产积极性,全国农业和农村经济形势出现了多年来少有的好形势,粮食产量达到4694.5亿kg,扭转了连续几年的粮食减产局面.农民人均收入达到2 936元,扣除物价上涨因素,增长率为6.8%.在这种形势下,中央再次提出把提高农业综合生产能力作为当前和今后一个时期农村工作的主题,其寓意深刻,意义重大.最近,对铁岭市辖区内的昌图、开原、铁岭县(市)进行了提高农业综合生产能力调研.从调查结果分析,在现有生产条件下,实现保持粮食稳定增产,农民持续增收,还面临着诸多制约因素,提高农业综合生产能力是一项长期而艰巨的任务.

关键词：农业综合生产能力铁岭市种植业粮食产量农产品产量调研报告粮食主产区

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对现代风险导向审计的一些认识

中国注册会计师 2006年第7期 36-38页

作者：侯艳华上海立信长江会计师事务所

笔者有幸参加了中注协去年在上海国家会计学院举办的“现代风险导向审计理论与实务”培训班，对现代风险导向审计有了更加系统和清晰的认识，审计观念上也有了更新。现将这种认识与大家共同分享。

关键词：风险导向审计现代上海国家会计学院审计理论审计观念培训班中注协实务

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辽宁省良种推广补贴项目管理呈现新特点

中国农业信息 2007年第11期 18-19页

作者：侯艳华辽宁省农村经济委员会种植业管理处

2007年,辽宁省承担农业部专用玉米良种推广补贴项目20万hm2,高油大豆良种推广补贴项目5.33万hm2。2007年各项目县在总结以往工作经验的基础上,进一步加大了工作力度,做到了品种、面积、技术、订单"四落实",统一

关键词：良种推广补贴农业部专用玉米辽宁省种子管理部门统一供种

来源：博看期刊

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胃溃疡患者给予加味左金汤对病情的影响

中外女性健康研究 2022年第7期 5-6,53页

作者：侯艳华曹县人民医院门诊部山东菏泽274400

目的:研究加味左金汤治疗胃溃疡的临床作用,以期为胃溃疡治疗方法提供一定参考。方法:以80名女性胃溃疡患者作为研究对象,随机分为对照组和研究组,对照组予以奥美拉唑、阿莫西林、克拉霉素三联疗法治疗,研究组在对照组的基础上给予加味... 详细信息

目的:研究加味左金汤治疗胃溃疡的临床作用,以期为胃溃疡治疗方法提供一定参考。方法:以80名女性胃溃疡患者作为研究对象,随机分为对照组和研究组,对照组予以奥美拉唑、阿莫西林、克拉霉素三联疗法治疗,研究组在对照组的基础上给予加味左金汤治疗,治疗时间为28d,比较两组患者的治疗有效率、血清中胃泌素水平及炎性因子水平、生活质量评分,分析加味左金汤对胃溃疡患者病情的影响。结果:研究组患者治疗有效率为95.0%,对照组患者治疗有效率为77.5%,两组治疗有效率具有显著性差异(P<0.05);两组患者经给药治疗后,胃泌素水平及炎性因子TNF-α、IL-6水平均有所下降,治疗后的研究组患者胃泌素、TNF-α、IL-6水平均显著低于对照组患者,差异具有统计学意义(P<0.01)。经治疗后的研究组患者情绪功能、生理功能、社会功能评分均显著高于对照组患者,具有显著性差异(P<0.05)。结论:加味左金汤可提高胃溃疡治疗有效率,显著降低胃溃疡患者血清中胃泌素、TNF-α、IL-6水平,改善患者的生活质量。

关键词：胃溃疡加味左金汤胃泌素