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Bone Research 2024年第1期12卷 122-136页

作者： Nan-Yu Zou Ran Liu Mei Huang Yu-Rui Jiao Jie Wei yangzi jiang Wen-Zhen He Min Huang Yi-Li Xu Ling Liu Yu-Chen Sun Mi Yang Qi Guo Yan Huang Tian Su Ye Xiao Wei-Shan Wang Chao Zeng Guang-Hua Lei Xiang-Hang Luo Chang-Jun Li Department of Endocrinology Endocrinology Research CenterXiangya Hospital of Central South UniversityChangshaHunan 410008China Department of Orthopaedics Xiangya Hospital of Central South UniversityChangshaHunan 410008China Department of Epidemiology and Health Statistics Xiangya School of Public HealthCentral South UniversityChangshaChina Hunan Key Laboratory of Joint Degeneration and Injury ChangshaHunan 410008China Key Laboratory of Aging-related Bone and Joint Diseases Prevention and Treatment Ministry of EducationXiangya HospitalCentral South UniversityChangshaChina School of Biomedical Sciences Institute for Tissue Engineering and Regenerative MedicineFaculty of MedicineThe Chinese University of Hong KongHong Kong SARChina Department of Orthopaedics The First Affiliated Hospital of Shihezi UniversityShiheziXinjiangChina National Clinical Research Center for Geriatric Disorders Xiangya HospitalCentral South UniversityChangshaHunan 410008China

Skeletal stem/progenitor cell(SSPC)senescence is a major cause of decreased bone regenerative potential with aging,but the causes of SSPC senescence remain unclear.In this study,we revealed that macrophages in calluses secrete prosenescent factors,including grancalcin(GCA),during aging,which triggers SSPC senescence and impairs fracture healing.Local injection of human rGCA in young mice induced SSPC senescence and delayed fracture repair.Genetic deletion of Gca in monocytes/macrophages was sufficient to rejuvenate fracture repair in aged mice and alleviate SSPC senescence.Mechanistically,GCA binds to the plexin-B2 receptor and activates Arg2-mediated mitochondrial dysfunction,resulting in cellular senescence.Depletion of Plxnb2 in SSPCs impaired fracture healing.Administration of GCA-neutralizing antibody enhanced fracture healing in aged mice.Thus,our study revealed that senescent macrophages within calluses secrete GCA to trigger SSPC secondary senescence,and GCA neutralization represents a promising therapy for nonunion or delayed union in elderly individuals.

关键词： healing union senescence

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Nanosecond pulsed electric fields prime mesenchymal stem cells to peptide ghrelin and enhance chondrogenesis and osteochondral defect repair in vivo

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Science China(Life Sciences) 2022年第5期65卷 927-939页

作者： Kejia Li Litong Fan Jianjing Lin Boon Chin Heng Zhantao Deng Qiujian Zheng Jue Zhang yangzi jiang Zigang Ge Department of Biomedical Engineering College of EngineeringPeking UniversityBeijing 100871China Arthritis Clinical and Research Center Peking University People’s HospitalBeijing 100044China Arthritis Institute Peking UniversityBeijing 100871China Peking University School of Stomatology Beijing 100081China Department of Orthopedics Guangdong Provincial People’s HospitalGuangdong Academy of Medical SciencesGuangzhou 510080China Institute of Biomechanics and Biomedical Engineering College of EngineeringPeking UniversityBeijing 100871China Institute for Tissue Engineering and Regenerative Medicine School of Biomedical SciencesFaculty of MedicineThe Chinese University of Hong KongHong Kong 999077China School of Biomedical Sciences Faculty of MedicineThe Chinese University of Hong KongHong Kong 999077China Department of Biomedical Engineering College of Future TechnologyPeking UniversityBeijing 100871China

Mesenchymal stem cells(MSCs) are important cell sources in cartilage tissue development and homeostasis,and multiple strategies have been developed to improve MSCs chondrogenic differentiation with an aim of promoting cartilage regeneration.Here we report the effects of combining nanosecond pulsed electric fields(ns PEFs) followed by treatment with ghrelin(a hormone that stimulates release of growth hormone) to regulate chondrogenesis of MSCs.ns PEFs and ghrelin were observed to separately enhance the chondrogenesis of MSCs,and the effects were significantly enhanced when the bioelectric stimulation and hormone were combined,which in turn improved osteochondral tissue repair of these cells within Sprague Dawley rats.We further found that ns PEFs can prime MSCs to be more receptive to subsequent stimuli of differentiation by upregulated Oct4/Nanog and activated JNK signaling pathway.Ghrelin initiated chondrogenic differentiation by activation of ERK1/2 signaling pathway,and RNA-seq results indicated 243 genes were regulated,and JAK-STAT signaling pathway was involved.Interestingly,the sequential order of applying these two stimuli is critical,with ns PEFs pretreatment followed by ghrelin enhanced chondrogenesis of MSCs in vitro and subsequent cartilage regeneration in vivo,but not vice versa.This synergistic prochondrogenic effects provide us new insights and strategies for future cell-based therapies.

关键词： mesenchymal stem cells nanosecond pulsed electric fields ghrelin chondrogenic differentiation

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“Slow walk”mimetic tensile loading maintains human meniscus tissue resident progenitor cells homeostasis in photocrosslinked gelatin hydrogel

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Bioactive Materials 2023年第7期25卷 256-272页

作者： Jing Sun Yau Tsz Chan Ki Wai Kevin Ho Li Zhang Liming Bian Rocky S.Tuan yangzi jiang Institute for Tissue Engineering and Regenerative Medicine School of Biomedical SciencesFaculty of MedicineThe Chinese University of Hong KongShatinHong Kong Special Administrative Region of China School of Biomedical Sciences Faculty of MedicineThe Chinese University of Hong KongShatinHong Kong Special Administrative Region of China Department of Orthopaedics&Traumatology Faculty of MedicineThe Chinese University of Hong KongAnd Prince of Wales HospitalShatinHong Kong Special Administrative Region of China Department of Mechanical and Automation Engineering Faculty of EngineeringThe Chinese University of Hong KongShatinHong Kong Special Administrative Region of China Department of Biomedical Engineering Faculty of EngineeringThe Chinese University of Hong KongShatinHong Kong Special Administrative Region of China Center for Neuromusculoskeletal Restorative Medicine Hong Kong Special Administrative Region of China

Meniscus,the cushion in knee joint,is a load-bearing tissue that transfers mechanical forces to extracellular matrix(ECM)and tissue resident cells.The mechanoresponse of human tissue resident stem/progenitor cells in meniscus(hMeSPCs)is significant to tissue homeostasis and regeneration but is not well understood.This study reports that a mild cyclic tensile loading regimen of~1800 loads/day on hMeSPCs seeded in 3-dimensional(3D)photocrosslinked gelatin methacryloyl(GelMA)hydrogel is critical in maintaining cellular homeostasis.Experimentally,a“slow walk”biomimetic cyclic loading regimen(10%tensile strain,0.5 Hz,1 h/day,up to 15 days)is applied to hMeSPCs encapsulated in GelMA hydrogel with a magnetic force-controlled loading actuator.The loading significantly increases cell differentiation and fibrocartilage-like ECM deposition without affecting cell viability.Transcriptomic analysis reveals 332 mechanoresponsive genes,clustered into cell senescence,mechanical sensitivity,and ECM dynamics,associated with interleukins,integrins,and collagens/matrix metalloproteinase pathways.The cell-GelMA constructs show active ECM remodeling,traced using a green fluorescence tagged(GFT)-GelMA hydrogel.Loading enhances nascent pericellular matrix production by the encapsulated hMeSPCs,which gradually compensates for the hydrogel loss in the cultures.These findings demonstrate the strong tissue-forming ability of hMeSPCs,and the importance of mechanical factors in maintaining meniscus homeostasis.

关键词： Human meniscus progenitor cells 3D cell-based constructs Biomimetic cyclic loading GelMA hydrogel Extracellular matrix

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Alternative polyadenylation events in epithelial cells sense endometritis progression in dairy cows

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Journal of Integrative Agriculture 2023年第6期22卷 1820-1832页

作者： Meagan J.STOTTS yangzi ZHANG Shuwen ZHANG Jennifer J.MICHAL Juan VELEZ Bothe HANS Martin MAQUIVAR Zhihua jiang Department of Animal Sciences and Center for Reproductive Biology Washington State UniversityPullmanWA 99164-7620USA Aurora Organic Farms PlattevilleCO 80651USA

Endometritis(inflammation of the endometrial lining) is one of the most devastating reproductive diseases in dairy cattle, resulting in substantial production loss and causing more than $650 million in lost revenue annually in the USA.We hypothesize that alternative polyadenylation(APA) sites serve as decisive sensors for endometrium health and disease in dairy cows. Endometrial cells collected from 18 cows with purulent vaginal discharge scored 0 to 2 were used for APA profiling with our whole transcriptome termini site sequencing(WTTS-seq) method. Overall, pathogens trigger hosts to use more differentially expressed APA(DE-APA), more intronic DE-APA, more DE-APA sites per gene and more DE-genes associated with inflammation. Host CD59 molecule(CD59), Fc fragment of IgG receptor IIa(FCGR2A), lymphocyte antigen 75(LY75) and plasminogen(PLG) may serve as initial contacts or combats with pathogens on cell surface, followed by activation of nuclear receptor subfamily 1 group H member 4(NR1H4) to regulate AXL receptor tyrosine kinase(AXL), FGR proto-oncogene, Src family tyrosine kinase(FGR), HCK protooncogene, Src family tyrosine kinase(HCK) and integrin subunit beta 2(ITGB2) for anti-inflammation. This study is the first to show significance of cilium pathways in endometrium health and animal reproduction. MIR21 and MIR30A would be perfect antagonistic biomarkers for diagnosis of either inflammation or anti-inflammation. These novel findings will set precedent for future genomic studies to aid the dairy industry develop new strategies to reduce endometritis incidence and improve fertility.

关键词： organic dairy endometritis alternative polyadenylation infection progression antagonistic biomarkers

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Additively manufactured biodegradable porous magnesium implants for elimination of implant-related infections:An in vitro and in vivo study

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Bioactive Materials 2022年第2期7卷 140-152页

作者： Kai Xie Nanqing Wang Yu Guo Shuang Zhao Jia Tan Lei Wang Guoyuan Li Junxiang Wu yangzi Yang Wenyu Xu Juan Chen Wenbo jiang Penghuai Fu Yongqiang Hao Shanghai Key Laboratory of Orthopaedic Implants Department of Orthopaedic SurgeryShanghai Ninth People’s HospitalShanghai Jiao Tong University School of MedicineShanghai200011China National Engineering Research Center of Light Alloy Net Forming&State Key Laboratory of Metal Matrix Composite Shanghai Jiao Tong UniversityShanghai200240China Musculoskeletal Tumor Center Peking University People’s Hospital100044BeijingChina Department of Orthopedics The First Affiliated Hospital of USTCDivision of Life Sciences and MedicineUniversity of Science and Technology of ChinaHefeiAnhui230001China Clinical and Translational Research Center for 3D Printing Technology Shanghai Ninth People’s HospitalShanghai Jiao Tong University School of MedicineShanghai200011China

Magnesium(Mg)alloys that have both antibacterial and osteogenic properties are suitable candidates for orthopedic implants.However,the fabrication of ideal Mg implants suitable for bone repair remains challenging because it requires implants with interconnected pore structures and personalized geometric shapes.In this study,we fabricated a porous 3D-printed Mg-Nd-Zn-Zr(denoted as JDBM)implant with suitable mechanical properties using selective laser melting technology.The 3D-printed JDBM implant exhibited cytocompatibility in MC3T3-E1 and RAW267.4 cells and excellent osteoinductivity in vitro.Furthermore,the implant demonstrated excellent antibacterial ratios of 90.0% and 92.1% for methicillin-resistant S.aureus(MRSA)and Escherichia coli,respectively.The 3D-printed JDBM implant prevented MRSA-induced implant-related infection in a rabbit model and showed good in vivo biocompatibility based on the results of histological evaluation,blood tests,and Mg2+deposition detection.In addition,enhanced inflammatory response and TNF-α secretion were observed at the bone-implant interface of the 3D-printed JDBM implants during the early implantation stage.The high Mg^(2+)environment produced by the degradation of 3D-printed JDBM implants could promote M1 phenotype of macrophages(Tnf,iNOS,Ccl3,Ccl4,Ccl5,Cxcl10,and Cxcl2),and enhance the phagocytic ability of macrophages.The enhanced immunoregulatory effect generated by relatively fast Mg^(2+)release and implant degradation during the early implantation stage is a potential antibacterial mechanism of Mg-based implant.Our findings indicate that 3D-printed porous JDBM implants,having both antibacterial property and osteoinductivity,hold potential for future orthopedic applications.

关键词： Magnesium implants 3D printing Implant-related infections Antibacterial activity

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液氮喷雾冷却换热特性实验研究

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南京航空航天大学学报 2022年第2期54卷 290-296页

作者：佘阳梓蒋彦龙苏州工业园区服务外包职业学院苏州215000 南京航空航天大学飞行器环境控制与生命保障工业和信息化部重点实验室南京210016

通过搭建液氮喷雾冷却实验台,研究了TG6.5型实心锥喷嘴的喷雾冷却特性,分析了临界点附近的过热度和热流密度以及过热度和换热系数的关系,讨论了喷雾面积和喷雾流量对换热效果的影响。结果表明:在换热过程中,随着热流密度的增加,过热度... 详细信息

通过搭建液氮喷雾冷却实验台,研究了TG6.5型实心锥喷嘴的喷雾冷却特性,分析了临界点附近的过热度和热流密度以及过热度和换热系数的关系,讨论了喷雾面积和喷雾流量对换热效果的影响。结果表明:在换热过程中,随着热流密度的增加,过热度变化可以分为缓慢增加、明显增加和急剧增加3个阶段,并且区间的分布和换热面积的大小关系密切。受核态沸腾过程中气泡的影响,出现最大换热系数时的过热度要小于达到临界热流密度时的过热度。增加喷雾流量能显著提高换热效果;增大热沉面积会降低单位面积内液滴颗粒冲击的频次和强度,换热效果下降。

关键词：液氮喷雾冷却换热特性实验研究

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乙烯装置急冷油粘度控制及减粘塔运行分析

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石油化工 2004年第3期33卷 258-262页

作者：蒋勇洪晓江扬子石油化工公司烯烃厂江苏南京210048

针对国内外急冷油减粘技术现状,分析了急冷油粘度增加对循环系统的危害,介绍了旋流分离技术应用于急冷油减粘的工艺过程,该技术克服了其它减粘方法存在的一些缺陷。指出提高减粘塔顶温度的同时增加参与减粘的急冷油喷淋量,降低减粘塔顶... 详细信息

针对国内外急冷油减粘技术现状,分析了急冷油粘度增加对循环系统的危害,介绍了旋流分离技术应用于急冷油减粘的工艺过程,该技术克服了其它减粘方法存在的一些缺陷。指出提高减粘塔顶温度的同时增加参与减粘的急冷油喷淋量,降低减粘塔顶温差、控制好塔釜液面是进一步优化减粘塔操作的关键。

关键词：乙烯装置急冷油粘度减粘塔

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PURA综合征一例

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中华围产医学杂志 2020年第5期23卷 345-347页

作者：江静波伍洋子深圳市儿童医院新生儿科 510083 深圳市儿童医院心内科 510083

本文报道1例PURA综合征足月新生儿。患儿以肌张力低下、呼吸窘迫、喂养困难、反应差为主要临床表现。采用遗传病全外显子组高通量测序技术进行相关基因检测,提示PURA基因5q31区带c.98dupG,p.(Gly34fs)移码突变,确诊PURA综合征。患儿随... 详细信息

本文报道1例PURA综合征足月新生儿。患儿以肌张力低下、呼吸窘迫、喂养困难、反应差为主要临床表现。采用遗传病全外显子组高通量测序技术进行相关基因检测,提示PURA基因5q31区带c.98dupG,p.(Gly34fs)移码突变,确诊PURA综合征。患儿随访半年,精神发育严重落后。对于新生儿或儿童期表现为肌张力减退、癫痫、神经系统发育迟缓的患儿,排除早产儿脑损伤、缺氧缺血性脑病、遗传代谢病后,需考虑PURA综合征可能,应尽早进行遗传学检测,以协助诊断。

关键词：脑疾病染色体缺失癫痫智力障碍 DNA结合蛋白质类转录因子突变全外显子组测序

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基于自发漏磁效应的桥梁主缆断丝检测试验研究

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市政技术 2023年第6期41卷 76-81页

作者：马帅飞林阳子江东梅雪菲北京中交桥宇科技有限公司北京100000 广州市开博桥梁工程有限公司广东广州518000

为实现大体积桥梁主缆体系的精确断丝无损检测,采用COMSOL有限元软件建立了漏磁仿真模型,基于自发漏磁效应进行了试验研究,并分析了不同断丝宽度和断丝数目的磁场变化规律。通过分析可知,有限元仿真结果与试验结果基本一致,除去背景场... 详细信息

为实现大体积桥梁主缆体系的精确断丝无损检测,采用COMSOL有限元软件建立了漏磁仿真模型,基于自发漏磁效应进行了试验研究,并分析了不同断丝宽度和断丝数目的磁场变化规律。通过分析可知,有限元仿真结果与试验结果基本一致,除去背景场后磁通密度信号明显增强,证明了主缆断丝检测方法的有效性和灵敏性;断丝信号峰谷值随着断丝数目的增加而增大,同时信号的波谷间距也随着断丝宽度的增大而增加。基于自发漏磁效应的主缆断丝检测方法可以较好地发现缆索的细微断丝情况,为桥梁主缆体系无损检测提供了参考。

关键词：主缆断丝检测自发漏磁效应有限元仿真

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不连续焊接结构的强度研究

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石油化工设备 2004年第2期33卷 11-13页

作者：姜卫忠扬子石化检修安装有限责任公司江苏南京210048

在不停输带压开孔及封堵中 ,为了防止焊接时烧穿 ,常采用不连续焊 ,通过试验和数值模拟对采用不连续焊接结构的安全性进行了研究 ,得出了试验模型在运行过程中的应力分布状况和发生破坏的压力 ,并对试验和有限元结果进行了比较。

关键词：带压开孔不连续焊接结构试验研究数值模拟

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