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MULTI-TILLERING DWARF1, a new allele of BRITTLE CULM 12, affects plant height and tiller in rice

Science Bulletin 2016年第23期61卷 1810-1817页

作者： Haiping Yu Deyong Ren yangzhou zhu Jiangmin Xu Yuexing Wang Ruifang Liu Yunxia Fang Zhenyuan Shi Jiangjie Pan Mei Lu Bojun Ma Jiang Hu Yuchun Rao College of Chemistry and Life Sciences Zhejiang Normal University State Key Laboratory for Rice Biology China National Rice Research Institute Key Laboratory of Northeast Rice Biology and Breeding Ministry of Agriculture and Key Laboratory of Northern Japonica Rice Genetics and Breeding Ministry of EducationRice Research Institute Shenyang Agricultural University

Plant height and tillering are crucial factors determining rice plant architecture and influencing rice grain production. In this study, rnulti-tillering dwarf1 （mtdl）, a stable multi-tiller and dwarf mutant, was screened from the ethylmethane sulfonate-treated japonica rice variety Wuyunging7. Compared with the wild type, mtdl mutant exhibited pleiotropic phenotypes, including dwarf- ism, more tillers, brittle culms and delayed heading date. By employing map-based cloning strategy, the gene MTD1 was finally mapped to an approximately 66-kb region on the short arm of chromosome 9. Sequencing results showed that the gene LOCOsO9g02650 （BC12） in mtdl mutant had a single nucleotide substitution （G to A）, which gen- erated a premature translation stop. Over-expressing MTD1/BC12 coding sequ（nce rescued all the phenotypes of mtdl mutants including plant height and tillers, which confirms that BC12 is the mutated gene in mtdl mutant. Quantitative reverse tran,-eription-PCR analysis showed that MTDI/BCI2 could negatively regulate the expression of MONOCULM 1, IDEAL PLANT ARCHITECTURE1 and Tillering and Dwarf 1, and control rice tillering. Remark- ably, a-amylase activity analysis and gibberellic acid （GA） treatment showed that the dwarf phenotype of mtdl mutant was dependent on GA biosynthesis pathway. These results facilitated to further uncover the molecular mechanism of the growth and development in rice.

关键词： Rice - Dwarf Tillering - mtdl Gene cloning

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A meta-analysis of the impacts of forest logging on soil CO2 efflux

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Research in Cold and Arid Regions 2020年第3期12卷 165-179页

作者： LongFei Chen yangzhou Xiang ZhiBin He Jun Du PengFei Lin Xi zhu Linze Inland River Basin Research Station Chinese Ecosystem Research NetworkKey Laboratory of Eco-hydrology of Inland River BasinNorthwest Institute of Eco-Environment and ResourcesChinese Academy of SciencesLanzhouGansu 730000China School of Geography and Resources Guizhou Education UniversityGuiyangGuizhou 550018China

Soil CO2 efflux,the second largest flux in a forest carbon budget,plays an important role in global carbon *** logging is expected to have large effects on soil CO2 efflux and carbon sequestration in forest ***,a comprehensive understanding of soil CO2 efflux dynamics in response to forest logging remains elusive due to large variability in results obtained across individual ***,we used a meta-analysis approach to synthesize the results of 77 individual field studies to determine the impacts of forest logging on soil CO2 *** results reveal that forest logging significantly stimulated soil CO2 efflux of the growing season by 5.02%.However,averaged across all studies,nonsignificant effect was detected following forest *** large variation among forest logging impacts was best explained by forest type,logging type,and time since *** CO2 efflux in coniferous forests exhibited a significant increase(4.38%)due to forest logging,while mixed and hardwood forests showed no significant *** type also had a significant effect on soil CO2 efflux,with thinning increasing soil CO2 efflux by 12.05%,while clear-cutting decreasing soil CO2 efflux by 8.63%.The time since logging also had variable effects,with higher soil CO2 efflux for 2 years after logging,and lower for 3-6 years after logging;when exceeded 6 years,soil CO2 efflux *** significantly negative impacts of forest logging were detected on fine root biomass,the general positive effects on soil CO2 efflux can be explained by the accelerated decomposition of organic matter as a result of elevated soil temperature and organic substrate *** results demonstrate that forest logging had potentially negative effects on carbon sequestration in forest ecosystems.

关键词： soil respiration forest ecosystem thinning clear-cutting time since logging

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Cytological identification of an isotetrasomic in rice and its application to centromere mapping

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Cell Research 1997年第1期7卷 31-38页

作者： CHENG zhu KUAN HENG XIU YU CHANG JIE YAN LI HUANG zhu MING HONG GU(Department of Agronomy, Agriculture College, yangzhou University, yangzhou 225009)(Institute of Genetics, Chinese Academy of Sciences,Beijing 100101) Department of Agronomy Agriculture College Yangzhou University Yangzhou Institute of Genetics Chinese Academy of Sciences Beijing

The aneuploid with isochromosome or telochromosome is ideal material for exploring the position of centromere in lingkage map. FOr obtaining these aneuploids in rice, the primary trisomics from triplo-1 to triplo-12 and the aneuploids derived from a triploid of indica rice variety Zhongxian 3037 were carefully investigated. From the offsprings of triplo-10, a primary trisomic of chromosome 10 of the variety, an isotetrasomic "triplo-10-1" was obtained. Cytological investigation revealed that a pair of extra isochromosomes of triplo-10-1 were come from the short arm of chromosome 10. In the offsprings of the isotetrasomic, a secondary trisomic "triplo-10-2" 5 in which the extra- chromosome was an isochromosome derived from the short arm of chromosome 10, was identified. With the isotetrasomic, secondary trisomic, primary trisomic and diploid of variety Zhongxian 3037, different molecular markers were used for exploring the position of the centromere of chromosome 10. Based on the DNA dosage effect, it was verified that the molecular markers G1125, G333 and L169 were located on the short arm, G1084 and other 16 available molecular markers were on the long arm of chromosome 10. So the centromere of chromosome 10 was located somewhere betweenG1125 and G1084 according to the RFLP linkage map given by Kurata et al[1]. The distance from G1125 to G1084 was about 3.2cM.

关键词： Rice isotetrasomic centromere mapping

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MMInstruct: a high-quality multi-modal instruction tuning dataset with extensive diversity

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Science China(Information Sciences) 2024年第12期67卷 36-51页

作者： yangzhou LIU Yue CAO Zhangwei GAO Weiyun WANG Zhe CHEN Wenhai WANG Hao TIAN Lewei LU Xizhou zhu Tong LU Yu QIAO Jifeng DAI School of Computer Science Nanjing University School of Electronic Information and Electrical Engineering Shanghai Jiao Tong University Shanghai AI Laboratory School of Computer Science Fudan University Department of Information Engineering The Chinese University of Hong Kong SenseTime Research Department of Electronic Engineering Tsinghua University

Despite the effectiveness of vision-language supervised fine-tuning in enhancing the performance of vision large language models(VLLMs), existing visual instruction tuning datasets include the following limitations.(1) Instruction annotation quality: despite existing VLLMs exhibiting strong performance,instructions generated by those advanced VLLMs may still suffer from inaccuracies, such as hallucinations.(2) Instructions and image diversity: the limited range of instruction types and the lack of diversity in image data may impact the model's ability to generate diversified and closer to real-world scenarios outputs. To address these challenges, we construct a high-quality, diverse visual instruction tuning dataset MMInstruct,which consists of 973k instructions from 24 domains. There are four instruction types: judgment, multiplechoice, long visual question answering, and short visual question answering. To construct MMInstruct, we propose an instruction generation data engine that leverages GPT-4V, GPT-3.5, and manual correction. Our instruction generation engine enables semi-automatic, low-cost, and multi-domain instruction generation at 1/6 the cost of manual construction. Through extensive experiment validation and ablation experiments,we demonstrate that MMInstruct could significantly improve the performance of VLLMs, e.g., the model fine-tuning on MMInstruct achieves new state-of-the-art performance on 10 out of 12 benchmarks. The code and data shall be available at https://***/yuecao0119/MMInstruct.

关键词： instruction tuning multi-modal multi-domain dataset vision large language model

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Fine mapping and cloning of MT1,a novel allele of D10

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Progress in Natural Science:Materials International 2009年第12期19卷 1683-1689页

作者： Yong Zhou,Jinyan zhu,Zhengyi Li,Fei Gu,Honggen Zhang,Shuzhu Tang, Minghong Gu,Guohua Liang~* Jiangsu Provincial Key Laboratory of Crop Genetics and Physiology,Key Laboratory of Plant Functional Genomics of Ministry of Education, yangzhou University,yangzhou 225009,China Jiangsu Provincial Key Laboratory of Crop Genetics and Physiology Key Laboratory of Plant Functional Genomics of Ministry of Education Yangzhou University Yangzhou 225009 China

Rice tillering is an important determinant for grain *** investigate the mechanism of tillering,we characterized a multiple tillering mutant(mt1) identified from the japonica variety,Zhonghua 11,treated with *** mutant exhibits advanced tillering development and dwarfed compared with wild-type *** analysis and fine gene mapping indicated that the mt1 mutant was controlled by a recessive gene,residing on a 29-kb window on AP003376 of chromosome *** putative gene in this region,encoding a carotenoid cleavage dioxygenase 8(CCD8),was allelic to *** mt1 mutant phenotype was complemented by introduction of wild-type MT1,and knockdown of MT1 in wild-type rice mimicked the mutant ***-time PCR analysis indicated that the MT1 gene is expressed highly in stems and at a low level in axillary buds,panicles,leaves,and *** addition,MT1 expression is clearly under feedback regulation.

关键词： Rice Tillering Map-based cloning OsCCD8 Feedback regulation

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Soil salinization research in China： Advances and prospects

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Journal of Geographical Sciences 2014年第5期24卷 943-960页

作者： LI Jianguo PU Lijie HAN Mingfang zhu Ming ZHANG Runsen XIANG yangzhou School of Geographic and Oceanographic Sciences Nanjing University Nanjing 210023 China Key Laboratory of Coastal Zone Exploitation and Protection Ministry of Land and Resources Nanjing210023 China Guizhou Institute of Forest Inventory and Planning Guiyang 550003 China

With the environmental deterioration caused by the advance of climate change, soil salinization is a serious and growing global problem. Currently about 7% of the world＇s land surface is threatened by salinization. China is a country whose soils are severely affected by this problem, which, due to its extensive area, and wide distribution poses a serious threat to regional agricultural development. In this review, we summarize the framework for soil salinization research in China over the past 70 years, assess the weaknesses of existing research in both a domestic and international context, highlight the trends and key findings of global research about saline soils over the past 30 years, and propose six major fields and directions for future research on saline soil.

关键词： saline soil research framework advances prospects

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Source-sink Characteristics and the Translocation of Assi- milates in New Plant Type and Intersubspecific Hybrid Rice

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Agricultural Sciences in China 2002年第2期1卷 155-162页

作者： YANG Jian-chang, ZHANG Wen-hu, WANG Zhi-qin, LIU Li-jun and zhu Qing-sen( Key Laboratory of Crop Cultivation and Physiology, MOA , yangzhou University, yangzhou 225009) Key Laboratory of Crop Cultivation and Physiology MOA Yangzhou University Yangzhou 225009

Both new plant type (NPT) and intersubspecific hybrid rice (IHR) had large sink size (total number of spikelets per m2), however, poor grain filling limited their potential in the grain yield. Compared to the three-line indica hybrid of Shanyou 63 (CK), NPT and IHR showed higher photosynthetic potential, higher dry matter accumulation and higher ratio of dry weight to spikelets (total dry wt./total number of spikelets) from heading to harvest. But both exhibited a low export percentage and transfer ratio of assimilates, low partitioning of 14C to grains from labeled flag leaves, low harvest indices and low physiological activities (IAA content and activities of ATPase and starch synthase) of grains at early grain-filling stage. The physiological activities of grains at early filling stage were significantly correlated with the export percentage and transfer ratio of assimilates, ripened-grain percentage and grain plumpness (r = 0.85 - 0.95). The source-sink ratio (dry matter wt./spikelet and nonstructural carbohydrate/spikelet) at heading was positively correlated with physiological activities of grains (r = 0. 84 - 0. 97 ). It is suggested that low physiological activities of grains at early filling stage is attributed to low source-sink ratio at heading, and the low sink activity weakens the ability to remobilize assimilates into grains, and leads to poor grain filling in NPT and IHR.

关键词： Rice New plant type Intersubspecifiic hybrid Source-sink Assimilate translocation

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工程措施对水泵进水条件影响的试验研究

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水力发电学报 2004年第4期23卷 69-73页

作者：朱红耕扬州大学水利科学与工程学院江苏大学流体中心镇江212013

立柱、底坎等工程措施常用于泵站前池和进水池流态整治 ,但对水泵进水条件的影响没有进行过定量的研究。本文采用旋度计测量水泵吸水管内的涡流强度 ,根据涡角的大小 ,定量地评价立柱、底坎、ω形后壁等工程措施对水泵进水条件的影响。... 详细信息

立柱、底坎等工程措施常用于泵站前池和进水池流态整治 ,但对水泵进水条件的影响没有进行过定量的研究。本文采用旋度计测量水泵吸水管内的涡流强度 ,根据涡角的大小 ,定量地评价立柱、底坎、ω形后壁等工程措施对水泵进水条件的影响。试验结果表明 ,在一定的条件下 ,立柱和底坎能改善水泵的进水条件 ,但需优化立柱和底坎的设计参数及位置 ,以期获得较好的整流效果 :“ω”形后壁配合导水锥能大幅度地降低吸水管内的涡流强度。

关键词：水力学水泵进水条件工程措施涡角旋度计

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Polyamines in developing rice grains and their relations with grain filling

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Chinese Rice Research Newsletter 1996年第4期4卷 4-5页

作者： YANG Jianchang,zhu Qingsen,WANG Zhiqin,and CAO Xianzu,Agri College of yangzhou Univ,yangzhou 225009,China

Many studies have reported that polyamines have an effect on a number of growth and developmental process in plants, such as cell division, embryogenesis, senescense, and flowering. However, there are few reports on the physiological significance in of polyamines grain filling of rice. We studied the level of polyamines in the developing grains and their relations with grain filling and grain weight, using 8 rice varieties including hybrid combinations. It was showed that the content of Put (puterscine), Spd (spermidine), and Spm (spermine), especially Spd and Spm, was

关键词： Polyamines in developing rice grains and their relations with grain filling Spd

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Genetic analysis of quality traits in intersubspecies crosses of rice

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Chinese Rice Research Newsletter 1997年第2期5卷 3-4页

作者： XU Chenwu Z HANG Aihong AO Yan zhu Qingsen Agri College,yangzhou Univ,yangzhou 225009,China

Indica and japonica rices are two subspecies ofAsia cultivated *** grain qualities exista significant *** objective of thispaper is to study the genetic control of qualitytraits in intersubspecies crosses of *** 1991,4 japonica varieties with widecompatibility,PC311,Zhenxiqiuguang,Lun-hui 422,and Ce 03 were crossed with 5 indicavarieties,3037,Zaoxiandang 18,IR36,Minghui 63,and Yangdao 4 according to NC(?)*** 20 hybrids using japonica as ma-

关键词： Genetic analysis of quality traits in intersubspecies crosses of rice

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