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Enhancement of Extracts from Celastrus orbiculatus on Maturation and Function of Dendritic Cells in vitro and in vivo

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Chinese Herbal Medicines 2010年第3期2卷 195-203页

作者： QIAN Ya-yun1, ZHANG Hua2. YUAN Lin1,3, HOU Ying1, LIU Wei-wei1, LIU Yan-qing1 1. Institute of Traditional Chinese Medicine & Western Medicine, yangzhou University, yangzhou 2.5009, China 2. Department of nursing, Medical College, yangzhou University, yangzhou 2.5009, China 3. Department of Image, 97th Hospital of People’s Liberation Army, Xuzhou 2.1000, China Institute of Traditional Chinese Medicine & Western Medicine Yangzhou UniversityYangzhou 225009China Department of nursing Medical CollegeYangzhou UniversityYangzhou 225009China Institute of Traditional Chinese Medicine & Western Medicine Yangzhou UniversityYangzhou 225009China Department of Image 97th Hospital of People's Liberation ArmyXuzhou 221000China

Objective To examine the immunoregulation of Celastrus orbiculatus extracts (COE), a traditional Chinese medicine, on maturation and function of dendritic cells (DCs) in vitro and in vivo. Methods In vitro, after treated with COE in different nontoxic concentrations (0, 10, 2., 40, 80, and 160 μg/mL) for 5 d, the surface immunological molecules and cytokine secretion of mice bone marrow-derived DCs in response to COE were analyzed by flow cytometric analysis (FACS) and enzyme linked immunosorbent assay (ELISA), respectively. In vivo, mouse hepatoma cells (Hepa1-6, 1 × 106) were injected sc and were treated with different dosages of COE (10, 2. or 40 mg/kg/d). Effects on tumor growth were determined by tumor volume and histology analysis after 2. d administration of COE. The relative proportions of mature DCs and CD8+ T cells were measured in mononuclear cells that had been isolated from spleen by FACS. Results COE stimulated IL-2.and IFN-γ secretion of DCs, simultaneously enhanced the maturation of DCs by enhancing immunological molecule (CD40, CD80, CD86, H-2.b, and I-Ab) expression in a dose-dependent manner. Furthermore, the chemotactic responses of DCs were significantly higher in COE-treated than untreated DCs, in association with higher chemokine receptor 7 expression. Furthermore, COE increased DCs produce IFN-γ and IL-2.in a dose-dependent manner when the concentration of COE less than 40 μg/mL, decreased DCs produce IL-10 and IL-4 also in a dose-dependent manner. In in vivo studies, COE can not only suppress growth of malignant hepatocellular carcinomas but also stimulate maturation of DCs, associated with strongly enhanced CD8+ CTL responses. Conclusion These data provide new insight into the mechanism of action of COE and indicate that the stimulation of maturation and function of DCs by COE contributes to its immunoregulatory effects.

关键词： antitumor Celastrus orbiculatus dendritic cell immunoregulation

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童心的探寻——幼教建筑的创作构思

泰州职业技术学院学报 2002年第4期2卷 6-8页

作者：陈骁张伟扬州市建筑设计研究院江苏扬州225000 扬州大学水利与建筑工程学院江苏扬州225000

通过研究幼儿的行为心理特点和规律 ,运用充满“童真”的建筑符号 ,组织灵活的游戏和学习场所 ,建立适宜的空间尺度 ,创造了富有“童心”和“童趣”的建筑作品。

关键词：童心幼教建筑创作构思

Correlation and Pathway Analysis of the Carbon,Nitrogen,and Phosphorus in Soil-Microorganism-Plant with Main Quality Components of Tea(Camellia sinensis)

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Phyton-International Journal of Experimental Botany 2024年第3期93卷 487-502页

作者： Chun Mao Ji He Xuefeng Wen yangzhou Xiang Jihong Feng Yingge Shu College of Agriculture Guizhou UniversityGuiyangChina School of Geography and Resources Guizhou Education UniversityGuiyangChina

The contents of carbon(C),nitrogen(N),and phosphorus(P)in soil-microorganisms-plant signiﬁcantly affect tea quality by altering the main quality components of tea,such as tea polyphenols,amino acids,and ***,few studies have quantiﬁed the effects of these factors on the main quality components of *** study aimed to explore the interactions of C,N,and P in soil-microorganisms-plants and the effects of these factors on the main quality components of tea by using the path analysis *** results indicated that(1)The contents of C,N,and P in soil,microorganisms,and tea plants were highly correlated and collinear,and showed signiﬁcant correlations with the main quality components of tea.(2.Optimal regression equations were established to esti-mate tea polyphenol,amino acid,catechin,caffeine,and water extract content based on C,N,and P contents in soil,microorganisms,and tea plants(R^(2.=0.92.,0.72.,0.954,0.848,and 0.883,respectively).(3)Pathway analysis showed that microbial biomass phosphorus(MBP),root phosphorus,branch nitrogen,and microbial biomass carbon(MBC)were the largest direct impact factors on tea polyphenol,catechin,water extracts,amino acid,and caffeine content,*** carbon,root phosphorus,and leaf nitrogen were the largest indirect impact factors on tea polyphenol,catechin,and water extract content,*** carbon indirectly affected tea polyphenol content mainly by altering MBP *** phosphorus indirectly affected catechin content mainly by altering soil organic carbon *** nitrogen indirectly affected water extract content mainly by altering branch nitrogen *** research results provide the scientiﬁc basis for reasonable fertilization in tea gardens and tea quality improvement.

关键词： Soil-microorganisms-plant system carbon nitrogen phosphorus tea quality path analysis

TRAIL诱导白血病细胞凋亡的分子机制研究

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实用临床医药杂志 2004年第3期8卷 36-40页

作者：祝瑾李宁川程宏扬州大学医学院江苏扬州225001 扬州大学体育学院江苏扬州225002

目的　检测TRAIL引起的T淋巴白血病细胞凋亡及与之相关的蛋白和信号通路 ,为探索T淋巴细胞凋亡分子机制打下基础 ,为相关肿瘤的治疗提供依据。方法　用TRAIL( 1μg/ml)刺激Jurkat细胞 3 6h以诱导细胞发生凋亡 ,用流式细胞术和MTS比色法... 详细信息

目的　检测TRAIL引起的T淋巴白血病细胞凋亡及与之相关的蛋白和信号通路 ,为探索T淋巴细胞凋亡分子机制打下基础 ,为相关肿瘤的治疗提供依据。方法　用TRAIL( 1μg/ml)刺激Jurkat细胞 3 6h以诱导细胞发生凋亡 ,用流式细胞术和MTS比色法检测细胞存活率 ,计算细胞凋亡率 ;用免疫印迹 (Westernblot)检测NF κB的表达和胱天肽酶 (caspase) 3、cas pase 8的变化。结果　TRAIL能诱导Jurkat细胞凋亡并伴随NF κB表达增加及caspase 3和caspase 8的活化。结论　TRAIL诱导的T淋巴白血病细胞凋亡的分子机制涉及NF κB及caspase 3、caspase 8,为TRAIL用于治疗白血病提供实验基础。

关键词：白血病 TRAIL 细胞凋亡 NF-κB 免疫印迹流式细胞术

Mapping and resource evaluation of deep high-temperature geothermal resources in the Jiyang Depression,China

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Energy Geoscience 2024年第4期5卷 142-155页

作者： Shaozheng Wang Yaoqi Zhou Xin Zhang yangzhou Wang Yonghong Yang Yuehan Shang Yang Chen Xiaoxin Shi School of Geosciences China University of Petroleum(East China)QingdaoShandong266580China Shandong Energy Group Co. Ltd.JinanShandong250101China Research Institute of Petroleum Exploration and Developement Shengli OilfieldDongyingShandong257000China

In China,geothermal resource utilization has mainly focused on resources at shallow and medium ***,the exploration of deep,high-temperature geothermal resources holds significant importance for achieving the“dual carbon”goals and the transition of energy *** Jiyang Depression in the Bohai Bay Basin has vast potential for deep,high-temperature geothermal *** analyzing data from 2.87 wells with temperature logs and 2.0 locations for temperature measurement in deep strata,we mapped the geothermal field of shallow to medium-deep layers in the Jiyang Depression using ArcGIS and predicted the temperatures of deep layers with a burial depth of 4000 *** stochastic modeling and numerical simulation,a reservoir attribute parameter database for favorable deep,high-temperature geothermal areas was developed,systematically characterizing the spatial distribution of geothermal resources within a play fairway of 139.5 km2.and estimating the exploitable deep geothermal resource potential by using the heat storage method and Monte Carlo data *** study reveals that the Fan 54 well block in the Boxing-Jijia region is of prime significance to develop deep,high-temperature geothermal resources in the Jiyang *** from the Cenozoic to the Upper Paleozoic are identified as effective cap layers for these deep geothermal *** Lower Paleozoic capable of effectively storing thermal energy and possessing an exploitable resource volume up to 12. million tons of standard coal,is identified as a target system for the development of deep high-temperature geothermal resources,providing significant insights for the efficient development of geothermal resources in the Jiyang Depression.

关键词： Deep high-temperature geothermal resource Geological modeling Resource assessment Lower Paleozoic Jiyang Depression

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造价工程师在承包商企业中的作用

泰州职业技术学院学报 2002年第4期2卷 16-18页

作者：余璠璟李建民扬州大学水利与建筑工程学院江苏扬州225009 泰兴市审计局江苏泰兴225400

本文讲述了世界经济一体化的形势下 ,服务于承包商的造价工程师在项目管理中的作用及主要工作内容、任务。

关键词：项目管理造价工程师投标报价成本管理风险管理

基于ASIT-UKF算法的锂电池荷电状态估计

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北京工业大学学报 2024年第6期50卷 683-692页

作者：陈阳舟伊磊北京工业大学信息学部北京100124

针对无迹卡尔曼滤波(unscented Kalman filter,UKF)算法估计锂电池荷电状态(state of charge,SOC)时精度低、稳定性差、产生的sigma点过多导致计算难度大等不足,提出一种基于自适应球形不敏变换方式的无迹卡尔曼滤波(unscented Kalman f... 详细信息

针对无迹卡尔曼滤波(unscented Kalman filter,UKF)算法估计锂电池荷电状态(state of charge,SOC)时精度低、稳定性差、产生的sigma点过多导致计算难度大等不足,提出一种基于自适应球形不敏变换方式的无迹卡尔曼滤波(unscented Kalman filter based on adaptive spherical insensitive transformation,ASIT-UKF)算法。该算法通过使用球形不敏变换方式选择权系数以及初始化一元向量对sigma点的产生进行选取。与UKF算法相比,ASIT-UKF算法产生的sigma点减少近50%,使得算法的计算复杂度大大降低。同时,将产生的所有sigma点进行单位球形面上的归一化处理,提高了数值的稳定性。考虑到实际运行中锂电池系统噪声干扰带来的不确定性,加入Sage-Husa自适应滤波器对不确定性噪声的干扰进行实时更新和修正,以达到提高在线锂电池SOC估计精度的目的。最后,将均方根误差和最大绝对误差计算公式引入到性能估计指标中。实验结果表明,ASIT-UKF算法在准确度、鲁棒性和收敛性方面具有优越的性能。

关键词：锂电池荷电状态(state of charge,SOC)估计球形不敏变换 Sage-Husa滤波无迹卡尔曼滤波(unscented Kalman filter,UKF)算法均方根误差

PCDH17 restricts dendritic spine morphogenesis by regulating ROCK2.dependent control of the actin cytoskeleton,modulating emotional behavior

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Zoological Research 2024年第3期45卷 535-550页

作者： Laidong Yu Fangfang Zeng Mengshu Fan Kexuan Zhang Jingjing Duan Yalu Tan Panlin Liao Jin Wen Chenyu Wang Meilin Wang Jialong Yuan Xinxin Pang Yan Huang yangzhou Zhang Jia-Da Li Zhuohua Zhang Zhonghua Hu Hunan Key Laboratory of Molecular Precision Medicine Department of Critical Care MedicineXiangya HospitalCentral South UniversityChangshaHunan 410008China Center for Medical Genetics and Hunan Key Laboratory of Medical Genetics School of Life SciencesCentral South UniversityChangshaHunan 410008China National Clinical Research Center for Geriatric Disorders Xiangya HospitalCentral South UniversityChangshaHunan 410008China Hunan Provincial Clinical Research Center for Critical Care Medicine Xiangya HospitalCentral South UniversityChangshaHunan 410008China Hunan Key Laboratory of Animal Models for Human Diseases School of Life SciencesCentral South UniversityChangshaHunan 410008China MOE Key Lab of Rare Pediatric Diseases School of Life SciencesCentral South UniversityChangshaHunan 410008China Department of Neurosciences University of South China Medical SchoolHengyangHunan 421001China

Proper regulation of synapse formation and elimination is critical for establishing mature neuronal circuits and maintaining brain *** abnormalities,such as defects in the density and morphology of postsynaptic dendritic spines,underlie the pathology of various neuropsychiatric *** 17(PCDH17)is associated with major mood disorders,including bipolar disorder and ***,the molecular mechanisms by which PCDH17 regulates spine number,morphology,and behavior remain *** this study,we found that PCDH17 functions at postsynaptic sites,restricting the number and size of dendritic spines in excitatory *** overexpression of PCDH17 in the ventral hippocampal CA1 results in spine loss and anxiety-and depression-like behaviors in ***,PCDH17 interacts with actin-relevant proteins and regulates actin filament(F-actin)***,PCDH17 binds to ROCK2.increasing its expression and subsequently enhancing the activity of downstream targets such as LIMK1 and the phosphorylation of cofilin serine-3(Ser3).Inhibition of ROCK2.activity with belumosudil(KD02.)ameliorates the defective F-actin organization and spine structure induced by PCDH17 overexpression,suggesting that ROCK2.mediates the effects of PCDH17 on F-actin content and spine ***,these findings reveal a novel mechanism by which PCDH17 regulates synapse development and behavior,providing pathological insights into the neurobiological basis of mood disorders.

关键词： Synapse development Dendritic spine Mood disorder Actin cytoskeleton Animal behavior

Nonlinear ergodic theorem for semitopological semigroups of non-Lipschitzian mappings in Banach spaces

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Chinese Science Bulletin 1997年第1期42卷 8-11页

作者： LI Gang and MA Jipu1. Department of Mathematics, yangzhou Normal College, yangzhou 2.5002. China 2. Department of Mathematics, Nanjing University, Nanjing 2.0093, China Department of Mathematics Yangzhou Normal College Yangzhou China Department of Mathematics Nanjing University Nanjing China

LET G be a semitopological semigroup, i. e. G is a semigroup with a Hausdorff topology such that for each s∈G the mapping s|→s·t and s|→t·s from G to G are continuous. G is called right reversible if any two closed left ideals of G have non-void intersection. In this case, (G,≤) is a directed system when the binary relation '≤' on G is defined by a≤b if and only if Let E be the uniformly convex Banach space

关键词： nonlinear semigroup ergodic theorem non-Lipschitzian mapping.