检索结果-南通市图书馆

Plant Communications 2023年第2期4卷 151-166页

作者： Yiqin Wang Zhenfeng Teng Hua Li Wei Wang Fan Xu Kai Sun Jinfang Chu yangwen qian Gary JLoake Chengcai Chu Jiuyou Tang State Key Laboratory of Plant Genomics Institute of Genetics and Developmental Biologythe Innovative Academy of Seed DesignChinese Academy of SciencesBeijing 100101China Institute of Genetics and Developmental Biology and National Center for Plant Gene Research(Beijing) Chinese Academy of SciencesBeijing 100101China Biogle Genome Editing Center Changzhou 213125China Institute of Molecular Plant Sciences School of Biological SciencesUniversity of EdinburghEdinburgh EH93BFUK Guangdong Laboratory for Lingnan Modern Agriculture State Key Laboratory for Conservation and Utilization of Subtropical Agro-BioresourcesCollege of AgricultureSouth China Agricultural UniversityGuangzhou 510642China

A key event that follows pathogen recognition by a resistance(R)protein containing an NB-ARC(nucleotide-binding adaptor shared by Apaf-1,R proteins,and Ced-4)domain is hypersensitive response(HR)-type cell death accompanied by accumulation of reactive oxygen species and nitric ***,the integral mechanisms that underlie this process remain relatively ***,we show that a gain-offunction mutation in the NB-ARC protein RLS1(Rapid Leaf Senescence 1)triggers high-light-dependent HR-like cell death in *** RLS1-mediated defense response is largely independent of salicylic acid accumulation,NPR1(Nonexpressor of Pathogenesis-Related Gene 1)activity,and RAR1(Required for Mla12 Resistance 1)function.A screen for suppressors of RLS1 activation identified RMC(Root Meander Curling)as essential for the RLS1-activated defense *** encodes a cysteine-rich receptor-like secreted protein(CRRSP)and functions as an RLS1-binding ***,their co-expression resulted in a change in the pattern of subcellular localization and was sufficient to trigger cell death accompanied by a decrease in the activity of the antioxidant enzyme ***,our findings reveal an NBARC-CRRSP signaling module that modulates oxidative state,the cell death process,and associated immunity responses in rice.

关键词： hypersensitive response NB-ARC protein cysteine-rich receptor-like secreted protein disease resistance antioxidant enzymes

A cryptic inhibitor of cytokinin phosphorelay controls rice grain size

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Molecular Plant 2022年第2期15卷 293-307页

作者： Dapu Liu He Zhao Yunhua Xiao Guoxia Zhang Shouyun Cao Wenchao Yin yangwen qian Yanhai Yin Jinsong Zhang Shouyi Chen Chengcai Chu Hongning Tong National Key Facility for Crop Gene Resources and Genetic Improvement Institute of Crop SciencesChinese Academy of Agricultural SciencesBeijing 100081China State Key Laboratory of Plant Genomics Institute of Genetics and Developmental Biologythe Innovative Academy for Seed DesignChinese Academy of SciencesBeijing 100101China Biogle Genome Editing Center ChangzhouJiangsu Province 213125China Department of Genetics Development and Cell BiologyIowa State UniversityAmesIA 50011USA

Plant hormone cytokinin signals through histidine-aspartic acid(H-D)phosphorelay to regulate plant growth and *** it is well known that the phosphorelay involves histidine kinases,histidine phosphotransfer proteins(HPs),and response regulators(RRs),how this process is regulated by external components remains *** we demonstrate that protein phosphatase with kelch-like domains(PPKL1),known as a signaling component of steroid hormone brassinosteroid,is actually a cryptic inhibitor of cytokinin phosphorelay in rice(Oryza sativa).Mutation at a specific amino acid D364 of PPKL1 activates cytokinin response and thus enlarges grain size in a semi-dominant mutant named *** of PPKL1 containing D364,either with the deletion of the phosphatase domain or not,rescues the s48 mutant ***1 interacts with OsAHP2,one of authentic HPs,and D364 resides in a region resembling the receiver domain of ***,PPKL1 can utilize D364 to suppress OsAHP2-to-RR phosphorelay,whereas mutation of D364 abolishes the *** function of PPKL1 is independent of the phosphatase domain that is required for brassinosteroid ***,editing of the D364-residential region produces a diversity of semi-dominant mutations associated with variously increased grain *** screening of the edited plants enables the identification of two genotypes that confer significantly improved grain ***,our study uncovers a noncanonical cytokinin signaling suppressor and provides a robust tool for seed rational design.

关键词： cytokinin brassinosteroid grain size signaling PPKL1 rice

Genome-wide Targeted Mutagenesis in Rice Using the CRISPR/Cas9 System

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Molecular Plant 2017年第9期10卷 1242-1245页

作者： Lu, Yuming Ye, Xiao Guo, Renming Huang, Jing Wang, Wei Tang, Jiuyou Tan, Longtao Zhu, Jian-kang Chu, Chengcai qian, yangwen Biogle Genome Editing Ctr Changzhou 213125 Jiangsu Peoples R China Chinese Acad Sci Natl Ctr Plant Gene Res Beijing Inst Genet & Dev Biol State Key Lab Plant Genom Beijing 100101 Peoples R China Chinese Acad Sci Shanghai Ctr Plant Stress Biol Shanghai 201602 Peoples R China Chinese Acad Sci Ctr Excellence Mol Plant Sci Shanghai 201602 Peoples R China Chinese Acad Sci Inst Subtrop Agr Key Lab Agroecol Proc Subtrop Reg Changsha 410125 Hunan Peoples R China

Dear Editor Since the completion of the rice （Oryza sativa） genome- sequencing project, a major goal of rice research has been the functional characterization of all annotated genetic loci in various biological processes. One of the most efficient and widely-used strategies for studying gene function is genetic mutagenesis. Several rice mutant libraries have been generated in the past decade, providing a wealth of resources for plant research （Chang et al., 2012）. CRISPR/Cas9 （clustered regularly interspaced short palindromic repeats-associated nuclease 9） has recently emerged as a powerful tool for rice research and breeding （Cong et al., 2013; Feng et al., 2013; Miao et al., 2013;

关键词： Genome-wide Targeted Mutagenesis Rice Using the CRISPR Cas9 System

Modulation of nitrate-induced phosphate response by the MYB transcription factor RLI1/HINGE1 in the nucleus

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Molecular Plant 2021年第3期14卷 517-529页

作者： Zhihua Zhang Zhao Li Wei Wang Zhimin Jiang Liping Guo Xiaohan Wang yangwen qian Xiahe Huang Yongqiang Liu Xiujie Liu Yahong Liu Aifu Li Yu Yan Junpeng Xie Shouyun Cao Stanislav Kopriva Legong Li Fanjiang Kong Baohui Liu Yingchun Wang Bin Hu Chengcai Chu State Key Laboratory of Plant Genomics Institute of Genetics and Developmental Biologythe Innovative Academy for Seed DesignChinese Academy of SciencesBeijingChina School of Life Sciences Guangzhou University.GuangzhouChina College of Plant Science Jilin UniversityChangchunChina Biogle Genome Editing Center ChangzhouChina State Key Laboratory of Molecular Developmental Biology Institute of Genetics and Developmental Biologythe Innovative Academy for Seed DesignChinese Academy of SciencesBeijingChina College of Advanced Agricultural Science University of Chinese Academy of SciencesBeijingChina Botanical Institute Cluster of Excellence on Plant SciencesUniversity of CologneCologneGermany College of Life Sciences Capital Normal UniversityBeijingChina

The coordinated utilization of nitrogen(N)and phosphorus(P)is vital for plants to maintain nutrient balance and achieve optimal ***,we revealed a mechanism by which nitrate induces genes for phosphate utilization;this mechanism depends on NRT1.1B-facilitated degradation of cytoplasmic SPX4,which in turn promotes cytoplasmic-nuclear shuttling of PHR2,the central transcription factor of phosphate signaling,and triggers the nitrate-induced phosphate response(NIPR)and N-P coordinated utilization in *** this study,we unveiled a fine-tuning mechanism of NIPR in the nucleus regulated by Highly Induced by Nitrate Gene 1(HINGE1,also known as RLI1),a MYB-transcription factor closely related to ***1/HINGE1,which is transcriptionally activated by PHR2 under nitrate induction,can directly activate the expression of phosphate starvation-induced *** importantly,RLI1/HINGE1 competes with PHR2 for binding to its repressor proteins in the nucleus(SPX proteins),and consequently releases PHR2 to further enhance phosphate ***,RLI1/HINGE1 amplifies the phosphate response in the nucleus downstream of the cytoplasmic SPX4-PHR2 cascade,thereby enabling fine-tuning of N-P balance when nitrate supply is sufficient.

关键词： rice NIPR RLI1/HINGE1 PHR2 SPXs nucleus

A rice TPR domain protein BSR-K1 negatively regulates broad-spectrum resistance via binding OsPAL1 mRNA

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

A rice TPR domain protein BSR-K1 negatively regulates broad-...

中国植物病理学会2019年学术年会

作者： Zhou Xiaogang Liao Haicheng Chern Mawsheng Yin Junjie Chen Yufei Wang Jianping Zhu Xiaobo Chen Zhixiong Yuan Can Zhao Wen Wang Jing Li Weitao He Min Ma Bingtian Wang Jichun Qin Peng Chen Weilan Wang Yuping Liu Jiali qian yangwen Wang Wenming Wu Xianjun Li Ping Zhu Lihuang Li Shigui Pamela C.Ronald Chen Xuewei Key Laboratory of Major Crop Diseases Sichuan Agricultural University State Key Laboratory of Hybrid Rice Sichuan Agricultural University

Crops carrying broad-spectrum resistance loci provide an effective strategy for controlling infectious disease because these loci typically confer resistance to diverse races of a pathogen or even multiple species of *** their importance,only a few crop broad-spectrum resistance loci have been ***,we report the identification and characterization of the ricebsr-kl(broad-spectrum resistance Kitaake-1) mutant,which confers broad-spectrum resistance against Magnaporthe oryzae and Xanthomonas oryzae *** with no major penalty on key agronomic ***-based cloning reveals that Bsr-k1 encodes a tetratricopeptide repeats(TPRs)-containing protein,which binds to mRNAs of multiple OsPAL(OsPAL1-7) genes and promotes their *** of function of the Bsr-k1 gene leads to accumulation of OsPAL1-7 mRNAs in the bsr-k1 ***,overexpression of OsPAL1 in wild-type rice TP309 confers resistance to ***,supporting the role of *** discovery of the bsr-k1 allele constitutes a significant conceptual advancement and provides a valuable tool for breeding broad-spectrum resistant rice.

关键词：

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

配方施肥对薄壳山核桃生长及土壤性状的影响

西南大学学报（自然科学版） 2025年第1期47卷 178-188页

作者：杜洋文周倩杜拾平程军勇张荣洋湖北省林业科学研究院武汉430075 湖北省林木种苗管理总站武汉430079 京山市虎爪山林场湖北荆门431815

为探索薄壳山核桃幼林生长发育的适宜施肥配方,采用氮、磷、钾3因素4水平16个处理的正交试验设计,探究不同施肥配方对薄壳山核桃幼林生长、土壤酶活性、土壤养分含量及叶片矿质养分含量的影响。结果表明:影响幼林树高和茎粗增长量大小... 详细信息

为探索薄壳山核桃幼林生长发育的适宜施肥配方,采用氮、磷、钾3因素4水平16个处理的正交试验设计,探究不同施肥配方对薄壳山核桃幼林生长、土壤酶活性、土壤养分含量及叶片矿质养分含量的影响。结果表明:影响幼林树高和茎粗增长量大小的肥料主次顺序为:尿素、氯化钾、过磷酸钙,最优水平组合为:尿素0.45 kg/株+过磷酸钙1.71 kg/株+氯化钾0.28 kg/株;影响叶片氮、磷、钾含量高低的肥料主次顺序为:氯化钾、尿素、过磷酸钙,最优水平组合为:尿素0.11 kg/株+过磷酸钙0.85 kg/株+氯化钾0.46 kg/株;影响土壤有效氮、磷、钾及有机质含量高低的肥料主次顺序为:尿素、氯化钾、过磷酸钙,最优水平组合为:尿素0.33 kg/株+过磷酸钙1.28 kg/株+氯化钾0.28 kg/株;影响土壤酶活性高低的肥料主次顺序为:尿素、过磷酸钙、氯化钾,最优水平组合为:尿素0.22 kg/株+过磷酸钙0.85 kg/株+氯化钾0.28 kg/株。树高和茎粗增长量与叶片全氮和全磷含量存在显著负相关,土壤蔗糖酶与土壤有效氮、磷、钾存在显著正相关。总体上,对薄壳山核桃幼林生长发育影响的肥料主次顺序为:尿素、氯化钾、过磷酸钙,最优施肥水平组合为尿素0.45 kg/株+过磷酸钙0.85 kg/株+氯化钾0.28 kg/株。

关键词：配方施肥薄壳山核桃生长土壤酶土壤养分叶片养分

在线全文

学校读者我要写书评

暂无评论

杉木近缘优良无性系交配子代遗传效应研究

种子 2018年第8期37卷 41-46页

作者：杜凌史秀文陈波涛毛红龙倩石扬文潘震杨月吉张成杨新烟贵州省林业技术推广总站贵阳550001 贵州省黎平县国有东风林场贵州黎平557300 贵州省林业科学研究院贵阳550011

对2个原产贵州锦屏和3个原产贵州天柱的5个优良无性系开展全双列杂交,获得25份交配组合种子;对成功培育的19个杂交组合苗木,在贵州省黎平县布置随机区组造林设计试验测定林;应用14年生的试验测定林调查数据统计分析表型变异,线性模型分... 详细信息

对2个原产贵州锦屏和3个原产贵州天柱的5个优良无性系开展全双列杂交,获得25份交配组合种子;对成功培育的19个杂交组合苗木,在贵州省黎平县布置随机区组造林设计试验测定林;应用14年生的试验测定林调查数据统计分析表型变异,线性模型分析试验测定林的遗传变异;采用双列杂交第4种方法固定模式,估算5个亲本10个交配组合子代性状的遗传参数。结果表明:20个参试家系的14年生试验测定林生长表现较差,但存在丰富变异,家系间树高、胸径和单株材积差异极显著(p≤0.01),生长性状受较强程度的遗传控制,树高、胸径和材积的家系遗传力分别为0.60、0.64和0.67。5个亲本10个交配组合间的主要生长性状呈极显著水平,交配组合与区组的交互作用明显,树高显著(p≤0.05)而胸径和材积极显著;不同亲本交配组合间的特殊配合力(SCA)极显著,树高的一般配合力(GCA)极显著而胸径和材积的SCA差异不显著。在5个亲本中,120号亲本的GCA和SCA效应较优,其次是123号和88号;120号和89号是建立双系种子园的最优亲本,5个亲本的合理配置可营建3系~5系种子园。

关键词：杉木双列杂交遗传变异一般配合力特殊配合力优良组合优良亲本

在线全文

学校读者我要写书评

暂无评论

黔杉杂种种子园种子杉木良种选育

贵州林业科技 2024年第4期52卷 14-18页

作者：石扬文潘震陈波涛张成杨月吉邓蔚松龙倩毛红贵州省黎平县国有东风林场贵州黎平557300 贵州省林业科学研究院贵州贵阳550005 贵州省核桃研究所贵州贵阳550005

“黔杉杂种种子园种子”是贵州国家杉木良种基地营建的双系和多系种子园生产的第3代杉木良种,旨在充分利用杉木第2世代选育优良组合的特殊配合力及亲本优良结实特性,保障生产性种子园高产稳产。应用选育优良组合亲本的交配关联性和固定... 详细信息

“黔杉杂种种子园种子”是贵州国家杉木良种基地营建的双系和多系种子园生产的第3代杉木良种,旨在充分利用杉木第2世代选育优良组合的特殊配合力及亲本优良结实特性,保障生产性种子园高产稳产。应用选育优良组合亲本的交配关联性和固定排列,使用4个亲本穗条嫁接营建双系种子园,使用11个亲本穗条嫁接营建多系种子园。入园无性系的优良开花结实特性得到充分体现及验证,盛产期的双系和多系种子园种子产量是同期常规种子园的3.9~6.3倍;5~6年生自由授粉鲜球果出种率约4.0%,种子发芽率高达49%。营建的双系和多系种子园仅具有良种生产功能而无育种功能,自由授粉子代优良单株不能选入下一世代育种群体,营建自由授粉子代测定进一步验证良种现实增益并为疏伐改造及良种审定提供科学依据。

关键词：杉木双系种子园多系种子园良种选育

渗透胁迫对构树叶型以及叶片分布分形维数的影响

在线全文

学校读者我要写书评

暂无评论

林业与环境科学 2022年第6期38卷 109-115页

作者：龙硕张欢欣钱志良姚靖泓王洋文刘禹含孟森胡文涛华南农业大学林学与风景园林学院/广东省森林植物种质创新与利用重点实验室广东广州510642 中国林业科学研究院热带林业研究所/国家林业和草原局热带林业研究重点实验室广东广州510520

构树Broussonetia papyrifera具有良好的抗逆性,在受到逆境时自身形态建成发生改变来应对环境。研究通过对一年生构树幼苗进行干旱胁迫和盐胁迫,测定叶型、光合气体参数、黄酮含量并采用计盒法分析构树叶片分形维数,结果表明干旱胁迫和... 详细信息

构树Broussonetia papyrifera具有良好的抗逆性,在受到逆境时自身形态建成发生改变来应对环境。研究通过对一年生构树幼苗进行干旱胁迫和盐胁迫,测定叶型、光合气体参数、黄酮含量并采用计盒法分析构树叶片分形维数,结果表明干旱胁迫和盐胁迫都能显著减弱构树光合作用,相较于对照组,干旱胁迫与盐胁迫的构树叶片胞间CO_(2)浓度、气孔导度和净光合速率分别显著下降了23.41%与41.16%、62.50%与69.72%、16.43%与17.60%。盐胁迫导致构树深裂叶占比显著增大,较对照组增加了58.96%。两种胁迫都能增加构树正视图和俯视图的叶片分形维数,尤其是两种胁迫分别使正视图的分形维数显著提高了7.90%、11.58%。同时,盐胁迫使黄酮在老叶中显著降低了17.85%。因此,面临渗透胁迫时,构树通过改变叶片形状、增加叶片占据空间的有效性和减少黄酮在老叶中的积累来应对。

关键词：干旱胁迫盐胁迫叶型分形维数构树