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Strategies and prospects for melatonin to alleviate abiotic stress in horticultural plants

Horticultural Plant Journal 2024年第3期10卷 601-614页

作者： Xiaoyun Wang Meng Cao Hongxin Li Ying Liu Shuangxi Fan Na Zhang yangdong guo College of Horticulture China Agricultural UniversityBeijing100193China College of Plant Science and Technology Beijing University of AgricultureBeijing102206China Beijing Bei Nong Enterprise Management Co. Ltd.Beijing102206China Sanya Institute of China Agricultural University SanyaHainan572000China

Melatonin is a conserved pleiotropic molecule in animals and *** is involved in many development processes and stress responses;thus,exploring its function in plants,particularly in horticultural plants,has become a rapidly developing *** studies have revealed that phytomelatonin acts as a plant biostimulant and increase its tolerance to various abiotic stressors,including extreme temperature,drought,osmotic disturbance,heavy metals,and ultraviolet(UV).Melatonin appears to have roles in the scavenging of reactive oxygen species(ROS)and other free radicals,affecting the primary and secondary metabolism of plants,regulating the transcripts of stress-related enzymes and transcription factors,and crosstalk with other hormones under different environmental *** pleiotropy makes phytomelatonin an attractive regulator to improve resistance to abiotic stress in *** recent discovery of the potential phytomelatonin receptor CAND2/PMTR1 and the proposition of putative models related to the phytomelatonin signaling pathways makes phytomelatonin a new plant *** on relevant studies from our laboratory,this review summarizes the phytomelatonin biosynthetic and metabolic pathways in plants and the latest research progress on phytomelatonin in abiotic stress of horticultural *** study will provide a reference for elucidating the regulatory mechanism of phytomelatonin affecting the resistance to abiotic stress in plants.

关键词： Abiotic stress Biostimulant Horticultural plants Phytomelatonin

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Palm oil protectsα-linolenic acid from rumen biohydrogenation and muscle oxidation in cashmere goat kids

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Journal of Animal Science and Biotechnology 2021年第2期12卷 711-728页

作者： Xue Wang Graeme B.Martin Qi Wen Shulin Liu Yinhao Li Binlin Shi Xiaoyu guo Yanli Zhao yangdong guo Sumei Yan Inner Mongolia Key Laboratory of Animal Nutrition and Feed Science College of Animal ScienceInner Mongolia Agricultural UniversityHohhot 010018China Beijing Advanced Innovation Center for Food Nutrition and Human Health College of HorticultureChina Agricultural UniversityBeijing 100193China UWA Institute of Agriculture The University of Western Australia35 Stirling HighwayCrawleyWA 6009Australia

Background:In ruminants,dietary C18:3n-3 can be lost through biohydrogenation in the rumen;and C18:3n-3 that by-passes the rumen still can be lost through oxidation in muscle,theoretically reducing the deposition of C18:3n-3,the substrate for synthesis of poly-unsaturated fatty acids(n-3 LCPUFA)in *** vitro studies have shown that rumen hydrogenation of C18:3n-3 is reduced by supplementation with palm oil(rich in cis-9 C18:1).In addition,in hepatocytes,studies with neonatal rats have shown that cis-9 C18:1 inhibits the oxidation of C18:*** therefore seems likely that palm oil could reduce both rumen biohydrogenation of C18:3n-3 and muscle oxidation of C18:*** present experiment tested whether the addition of palm oil to a linseed oil supplement for goat kids would prevent the losses of C18:3n-3 and thus improve the FA composition in two muscles,Longissimus dorsi and Biceps *** investigate the processes involved,we studied the rumen bacterial communities and measured the mRNA expression of genes related to lipid metabolism in Longissimus *** 4-month-old castrated male Albas white cashmere kids were randomly allocated among three dietary *** three diets contained the same ingredients in the same proportions,but differed in their fat additives:palm oil(PMO),linseed oil(LSO)or mixed oil(MIX;2 parts linseed oil plus 1 part palm oil on a weight basis).Results:Compared with the LSO diet,the MIX diet decreased the relative abuandance of Pseudobutyrivibrio,a bacterial species that is positively related to the proportional loss rate of dietary C18:3n-3 and that has been reported to generate the ATP required for biohydrogenation(reflecting a decrease in the abundance of rumen bacteria that hydrogenate C18:3n-3 in MIX kids).In muscle,the MIX diet increased concentrations of C18:3n-3,C20:5n-3,C22:6n-3,and n-3 LCPUFA,and thus decreased the n-6/n-3 ratio;decreased the mRNA expression of CPT1β(a gene associated with fatty acid oxidation)and

关键词： Desaturases Docosahexaenoic acid Hydrogenation Goats Meat Oleic acid Oxidative stress

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Research Progress on Bolting and Flowering Characteristics of Cruciferous Vegetables

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Agricultural Biotechnology 2023年第6期12卷 6-11,18页

作者： Yue LIANG Xingsheng LI yangdong guo Xiaowei YUAN Huasheng Agriculture Group Co. Ltd.Qingzhou 262500China College of Horticulture China Agricultural UniversityBeijing 100193China

There are many varieties of vegetables in Cruciferae,which have a wide distribution and occupy an important position in the global vegetable *** bolting period is a crucial stage in the growth cycle of cruciferous plants,which directly affects the yield and quality of vegetable *** paper summarized the research progress on the physiological and biochemical characteristics,molecular genetic mechanisms and molecular markers of the flowering and bolting traits in cruciferous vegetables,in order to provide new ideas for revealing the regulatory mechanisms of flowering and bolting in cruciferous vegetables and to provide reference for the breeding of new varieties of cruciferous vegetables that are resistant to flowering.

关键词： Cruciferae Bolting Flowering

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Main Diseases of Zucchini and Their Prevention and Control Methods

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Plant Diseases and Pests 2023年第1期14卷 9-13,16页

作者： Xiaowei YUAN Yimin GU yangdong guo Xiping SUN Xingsheng LI Huasheng Seed Group Co. Ltd.Qingzhou 262500China College of Horticulture China Agricultural UniversityBeijing 100193China

This paper elaborates the symptom characteristics and control methods of virus diseases including squash leaf curl china virus(SLCCNV),watermelon mosaic virus(WMV),cucurbit chlorotic yellows virus(CCYV),cucumber mosaic virus(CMV)and zucchini yellow mosaic virus(ZYMV),as well as fungal diseases including zucchini powdery mildew,zucchini downy mildew and zucchini scab that harm zucchini production,in order to provide a theoretical basis and technical support for disease prevention and control of zucchini.

关键词： Zucchini Disease Virus disease Fungal disease Control

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A FMEA-Based Safety Analysis on EV's Breakdown in E/E System:a Case Study

A FMEA-Based Safety Analysis on EV's Breakdown in E/E System...

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2022中国汽车工程学会年会暨展览会

作者： guo yangdong Yuan Meng Xie Yulu guo Shanghua Si Huachao VOYAH Automotive Technology Co. Ltd.

Electric vehicles have been now occupying an increasing market volume in the recent *** its expansion,the breaking-down issues emerge *** EV customers complain the breakdowns have reduced their driving experience undesirably,especially when no dangers were perceived while the vehicle ***,little do they know that some failures might be easy to detect while the others remain hidden,let alone that breaking down also provides the highest safety level to their families,friends,and even *** this paper,the deeper reason of EV breakdowns would be elaborated,and most of the attentions would be paid on E/E system,which plays a significant role in electric vehicle's driving and controlling ***,Failure Mode and Effects Analysis(FMEA),one of the first line risk evaluation mechanism,would be employed on a typical failure,overheating,to complete its risk rating and prioritization *** FMEA modelling process,based on BMS structure,the potential fault effect is analyzed,the latent causes are listed,and the recommended safety measures are put *** the present EV technique is maturing gradually,the vehicle safety is a developable issue,for both OEMs and *** safety rating and quality assurance might mean some advanced performances need to be *** to make both ends meet is a tough game for designers,and requires more professional experts to trade-off carefully and logically.

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Isolation and Identification of Squash Leaf Curl China Virus in Zucchini

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Agricultural Biotechnology 2022年第5期11卷 1-3,14页

作者： Huixian SHI Yimin GU Xingsheng LI Xiping SUN yangdong guo Xiaowei YUAN Huasheng Seed Group Co. Ltd.Qingzhou 262500China College of Horticulture China Agricultural UniversityBeijing 100193China

[Objectives]In 2019,virus diseases occurred widely on zucchini planted in Shandong *** disease symptom was different from previous *** study aimed to identify the pathogen causing the zucchini virus disease.[Methods]Ten diseased zucchini leaves were collected in the field and used as materials for PCR and sequencing.[Results]PCR detection and sequencing showed that the nucleotide sequence of the amplified fragment had the highest identity with the squash isolate of squash leaf curl China virus(SLCCNV)(MW389919.1)in Guangdong *** were further designed for amplifying the full-length *** full-length DNA-A was 2730 bp(OM692270.1),and the full-length DNA-B was 2711 bp(OM692269.1).Through sequence alignment,it was found that the DNA-A sequence shared identity of 89.65%-99.42%with registered SLCCNV,and the identity with the SLCCNV-GDHY pumpkin isolate(MW389919.1)in Guangdong was the highest,at 99.42%.The DNA-B sequence was identical with registered SLCCNV in the range of 81.82%-97.29%,and the identity with the SLCCNV-GDHY pumpkin isolate(MW389918.1)in Guangdong,was the highest,at 97.29%.Therefore,it was speculated that SLCCNV is the pathogen of zucchini virus *** the virus was first found on zucchini in Shandong,it was named SLCCNV-SD.[Conclusions]This study provides materials for the research on the spread of SLCCNV in China and the analysis of population genetic characteristics,as well as a reference for the prevention and control of the virus in zucchini.

关键词： Zucchini Viral disease Squash leaf curl China virus

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RNA干扰在植物抗根结线虫病基因工程应用中的研究进展

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基因组学与应用生物学 2009年第1期28卷 167-173页

作者：牛俊海郭仰东简恒中国农业大学农学与生物技术学院蔬菜系北京100094 中国农业大学农学与生物技术学院植病系线虫学实验室北京100094

植物根结线虫病对农业生产的危害连年加重,以轮作和化学农药为主的传统防治难以满足现代农业生产的需要。以常规的抗性育种和表达外源蛋白为主的抗线虫转基因育种主要受限于抗性基因的匮乏。而近来RNA干扰技术的应用为抗线虫基因工程带... 详细信息

植物根结线虫病对农业生产的危害连年加重,以轮作和化学农药为主的传统防治难以满足现代农业生产的需要。以常规的抗性育种和表达外源蛋白为主的抗线虫转基因育种主要受限于抗性基因的匮乏。而近来RNA干扰技术的应用为抗线虫基因工程带来新的突破,通过构建RNA干扰载体,在转基因植物中表达寄生线虫重要基因的dsRNA或siRNA,并经口针取食被导入线虫体内,并引发线虫的系统性RNA干扰反应,导致其出现寄生、发育、代谢、运动等障碍甚至致死,从而使转基因植物实现对寄生线虫的抗性。本文综述了RNAi介导的抗根结线虫基因工程方面的研究进展,分析探讨了这种新的策略的特点并展望了它的应用前景。

关键词： RNA干扰双链RNA 根结线虫基因工程

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浅析城市园林设计中历史文化的挖掘与表达

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南方农业 2021年第29期15卷 96-97页

作者：郭扬东信丰县市政公用事业服务中心江西赣州341600

传承历史文化始终是城市园林景观设计的重要命题,目前迫切需要形成对历史文化的认同,还包括对历史文化的深入挖掘与合理运用。长期积淀的历史事件、建筑、典故都已植入人们脑海,园林设计需要避免设计的同化。基于更正历史文化借鉴的误... 详细信息

传承历史文化始终是城市园林景观设计的重要命题,目前迫切需要形成对历史文化的认同,还包括对历史文化的深入挖掘与合理运用。长期积淀的历史事件、建筑、典故都已植入人们脑海,园林设计需要避免设计的同化。基于更正历史文化借鉴的误区、挖掘历史文化表达形式,分析园林设计中历史文化表征、聚合、升华的对策。

关键词：城市园林设计历史文化价值挖掘表达

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转录因子SlMYB-related 2对番茄耐冷性的影响

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华北农学报 2022年第1期37卷 35-41页

作者：吴红红段学粉郭仰东张喜春北京农学院植物科学技术学院北京市蔬菜遗传育种与生物技术实验室北京102206 中国农业大学园艺学院北京100094

番茄属于冷敏感模式植物,在生长过程中极易受冷害进而影响其产量。为进一步揭示番茄植株抗寒的分子机制以及对选育番茄抗寒品种提供理论依据,以蔬菜遗传育种与生物技术实验室种质资源编号25的番茄品种为材料、番茄转录因子SlMYB-relate... 详细信息

番茄属于冷敏感模式植物,在生长过程中极易受冷害进而影响其产量。为进一步揭示番茄植株抗寒的分子机制以及对选育番茄抗寒品种提供理论依据,以蔬菜遗传育种与生物技术实验室种质资源编号25的番茄品种为材料、番茄转录因子SlMYB-related 2为研究对象,基于CRISPR/Cas9基因敲除载体,通过农杆菌转化法转化获得番茄阳性植株;构建VIGS瞬时沉默表达载体,以野生型和未插入目的基因片段的病毒转化植株为对照,将3组植株进行4℃(16 h光照/8 h黑暗处理,60%湿度)低温处理后,测定抗冷相关生理指标的含量,比较其耐寒性。结果表明,构建了CRISPR/Cas9基因敲除载体,共获得了CRISPR沉默阳性植株2株;构建了VIGS沉默表达载体,经低温处理后发现,随着低温处理时间的延长,SlMYB-related 2基因转化组番茄植株的脯氨酸和可溶性糖的增长趋势比对照番茄组(野生型携带TRV空载,CK)和野生组(WT)慢,且含量低于CK组和野生组,但丙二醛含量高于CK组和野生组,证明VIGS瞬时表达番茄植株抗寒性显著低于CK组和野生组。通过对基因瞬时沉默表达后番茄植株的抗寒性分析,发现SlMYB-related 2基因在低温胁迫中起到了正调控作用。

关键词：番茄 MYB转录因子 VIGS CRISPR/Cas9 沉默载体遗传转化

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腹筋对深梁受剪承载力影响的试验研究

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矿业研究与开发 2020年第6期40卷 68-72页

作者：张俊红李树山解伟郭扬东华北水利水电大学土木与交通学院河南郑州450011

在矿山选矿厂等大跨度厂房建设中需构筑深梁构件。为研究腹筋配筋率对深梁受剪承载力的影响,在集中荷载作用下对4根高强钢筋混凝土深梁进行了受剪性能试验。通过对4根深梁试件受力全过程特征、破坏形态和荷载-挠度变形曲线等变化规律的... 详细信息

在矿山选矿厂等大跨度厂房建设中需构筑深梁构件。为研究腹筋配筋率对深梁受剪承载力的影响,在集中荷载作用下对4根高强钢筋混凝土深梁进行了受剪性能试验。通过对4根深梁试件受力全过程特征、破坏形态和荷载-挠度变形曲线等变化规律的分析,明确了腹筋配筋率对深梁开裂荷载、极限荷载及破坏形态的影响。研究表明:小剪跨比深梁破坏模式为斜压破坏,受腹筋的配置与含量影响,斜压破坏表现为压杆压毁和压杆劈裂两种形态。竖向箍筋配筋率对受剪承载力影响很小。采用《混凝土结构设计规范》(GB 50010-2010)中深梁受剪承载力计算方法对4根试件梁进行计算,计算结果与试验值较为接近,验证了试验结果的可靠性。

关键词：深梁腹筋受剪承载力破坏形态

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