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miR-134通过下调叉头盒蛋白M1抑制肝细胞癌细胞增殖

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第三军医大学学报 2017年第1期39卷 1-8页

作者：姜珊熊浩君何家琳李波杨帆杨腾张艳何凤田第三军医大学基础医学部生物化学与分子生物学教研室重庆400038

目的探讨miR-134下调叉头盒蛋白M1(forkhead box M1,FOXM1)的机制及其影响肝细胞癌(hepatocellular carcinoma,HCC)细胞增殖的作用。方法应用CCK-8法检测miR-134对HCC细胞增殖的作用;经Western blot和Real-time PCR检测人正常肝细胞L02和5种HCC细胞系中FOXM1和miR-134的表达;用miR-134模拟物(miR-134 mimic)或miR-134抑制剂(miR-134 inhibitor)转染HCC细胞后,经Western blot和Real-time PCR检测FOXM1及其下游靶基因Cyclin D1的表达;应用生物信息学分析miR-134在人FOXM1 3'-UTR的可能结合位点,通过报告基因检测实验分析miR-134与FOXM1的3'-UTR的特异性结合;将miR-134 mimic和FOXM1表达质粒(无3'-UTR)共转染于HCC细胞后,经CCK-8法检测细胞的增殖情况。结果 miR-134可显著抑制HCC细胞增殖(P<0.05);与人正常肝细胞L02相比,miR-134在HCC细胞中的表达明显降低(P<0.05),而FOXM1蛋白表达明显增强,二者存在负相关(R2=0.705,P<0.05);miR-134可显著下调FOXM1蛋白及其下游靶基因Cyclin D1的表达(P<0.05);报告基因检测实验证实miR-134可特异性结合于FOXM1 mRNA的3'-UTR(P<0.01);细胞增殖实验检测结果显示,过表达缺失3'-UTR的FOXM1可显著减弱miR-134对HCC细胞增殖的抑制效应(P<0.05)。结论 miR-134通过靶向结合于FOXM1的3'-UTR而下调FOXM1蛋白的表达,从而抑制HCC细胞的增殖。

关键词：癌细胞增殖蛋白表达肝细胞癌 CyclinD1 HCC细胞 carcinoma CCK-8法人正常肝细胞

畸变磁通作用下变压器铁心模型损耗的实验研究与模拟分析

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电工电能新技术 2017年第3期36卷 65-69页

作者：刘洋杨富尧范亚娜马光韩钰陈新先进输电技术国家重点实验室全球能源互联网研究院北京102211 保定天威保变电气股份有限公司河北保定071056

对畸变磁通条件下产品级变压器铁心模型的损耗进行了实验研究,考察了谐波相位差变化对铁心损耗的影响。给出了一种能够考虑畸变磁通条件下铁心损耗的工程计算方法,该方法可有效地考虑局部磁滞回环对铁心损耗的影响。结合MagNet电磁分析... 详细信息

对畸变磁通条件下产品级变压器铁心模型的损耗进行了实验研究,考察了谐波相位差变化对铁心损耗的影响。给出了一种能够考虑畸变磁通条件下铁心损耗的工程计算方法,该方法可有效地考虑局部磁滞回环对铁心损耗的影响。结合MagNet电磁分析软件,计算了产品级变压器铁心模型的铁心损耗,通过对比实验结果证明了方法的有效性。

关键词：畸变磁通铁心损耗磁滞回环有限元分析

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内窥镜在眼科的应用现状和发展

国际眼科纵览 2012年第3期36卷 145-149页

作者：杨扬帆余敏斌中山大学中山眼科中心眼科学国家重点实验室广州510060

近20年来医用内窥镜技术发展迅速，目前应用于眼科手术治疗的内窥镜主要有应用于眼球内手术的眼内窥镜、应用于泪道手术的泪道内窥镜以及应用于视神经管减压术、泪囊鼻腔吻合术的鼻内窥镜。内窥镜手术已成为眼科手术的一个新的发展和重... 详细信息

近20年来医用内窥镜技术发展迅速，目前应用于眼科手术治疗的内窥镜主要有应用于眼球内手术的眼内窥镜、应用于泪道手术的泪道内窥镜以及应用于视神经管减压术、泪囊鼻腔吻合术的鼻内窥镜。内窥镜手术已成为眼科手术的一个新的发展和重要补充。本文就内窥镜在眼科应用的历史、眼内窥镜类型和工作原理、手术入路以及内窥镜在眼科手术中应用的适应证、疗效和并发症作一综述。

关键词：内窥镜眼科临床疗效并发症

Light tuning CO/H_(2)composition on Ag:Unraveling CO_(2)mass transfer and electron-phonon coupling in plasmon-enhanced electrocatalysis

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Nano Research 2025年第1期18卷 173-185页

作者： Rui Lin Dong fan Luca MBerger Thomas Possmayer Yu Zhou Jiexin Zhu Xingbao Chen Wen Luo Lars Allmendinger Andreas Tittl Leonardo de Souza Menezes Guillaume Maurin Shihe yang and Stefan AMaier Nanoinstitute Munich Faculty of PhysicsLudwig-Maximilians-Universität München80539 MunichGermany Institut Charles Gerhardt Montpellier UMR 5253 CNRS ENSCM UniversitéMontpellierPlace E.Bataillon34090 MontpellierFrance Guangdong Provincial Key Lab of Nano-Micro Material Research School of Advanced MaterialsPeking University Shenzhen Graduate SchoolShenzhen 518055China State Key Laboratory of Advanced Technology for Materials Synthesis and Processing Wuhan University of TechnologyWuhan 430070China Faculty of Chemistry and Pharmacy Ludwig-Maximilians-Universität München81377 MunichGermany Departamento de Física Universidade Federal de Pernambuco50670-901 Recife-PEBrazil School of Physics and Astronomy Monash University Clayton CampusMelbourneVictoria 3800Australia Department of Physics Imperial College LondonSW72AZ LondonUK

Plasmon-enhanced electrocatalysis(PEEC)is an emerging approach to mitigate CO_(2)*** mechanisms behind CO_(2)adsorption and reduction at the catalyst-electrolyte interface in PEEC still need to be further ***,we employ a well-defined Ag nanostructure to elucidate these pivotal *** shining light with wavelengths of 625,525,405 nm on Ag,an adjustable CO/H_(2)ratio from 35 to 1 can be *** reaction pathway changing under plasmonic excitation does not originate from the lowered CO_(2)mass transfer in the vicinity of Ag,as the electrochemical quartz crystal microbalance results unravel that a slightly elevated temperature in bulk electrolyte caused by light irradiation cannot weaken the CO_(2)adsorption at the Ag catalyst-electrolyte *** calculations reveal that optical excitation towards shorter wavelengths leads to a progressive lowered energy barrier for H_(2)formation together with an enhanced energy barrier for^(*)COOH *** thermodynamically suppressed,CO_(2)reduction can still be improved kinetically by optimizing the excitation wavelength and intensity,being accompanied with the enhanced *** absorption spectroscopy results further correlate the higher photocurrent with a prolonged electron-phonon coupling time,verifying that the improvement of CO_(2)reduction kinetics in PEEC can be realized by hot electron harnessing.

关键词： plasmonic catalysis photoelectrochemical CO_(2)reduction CO/H_(2)ratio hot electron generation electronphonon coupling thermal effect

维普期刊数据库评论

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TMN中Socket通信问题探讨

微机发展 2000年第2期10卷 61-63页

作者：杨振江杨帆西安交通大学计算机系统结构与网络研究所陕西省西安市710049

本文简要探讨了在TMN的开发设计中 ,用Socket技术在TCP/IP协议的基础上实现通信的方法 ,步骤 ,并给出了一个简单的采用数据报式Socket通信的实例。

关键词： TMN 电信管理网套接字 Socket通信

Soluble epoxide hydrolase:a next-generation drug target for Alzheimer's disease and related dementias

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Neural Regeneration Research 2025年第9期20卷 2585-2586页

作者： Andrew Gregory Chengyun Tang fan fan Department of Physiology Medical College of GeorgiaAugusta UniversityAugustaGAUSA

Alzheimer's disease(AD)and Alzheimer's diseaserelated dementias(ADRD)represent a significant public health challenge,with projections indicating a substantial increase in affected individuals due to the aging global *** the World Health Organization,AD/ADRD has affected more than 55 million individuals worldwide,with an additional 10 million cases diagnosed each year.

关键词： Alzheimer population drug

A novel approach to the attitude stabilization structure for ducted-fan operative aerial robots:Finding improvements for modeling error and strong external transient disturbance

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Chinese Journal of Aeronautics 2022年第2期35卷 250-264页

作者： Wei fan Bin XU Changle XIANG Yibo ZHANG Haiyang yang School of Mechanical Engineering Beijing Institute of TechnologyBeijing 100081China Beijing Institute of Technology Chongqing Innovation Center Chongqing 401135China School of Automation Beijing Institute of TechnologyBeijing 100081China

This research concerns a novel attitude stabilization structure for a ducted-fan aerial robot to work against modeling error and strong external transient disturbance,and it focuses on two main control targets:modeling error compensation,and the improvement of disturbance resistance along the rolling *** the first research objective,we proposed an adaptive nominal controller with the reconfigurable control law design based on the estimation of the modeling error found in the *** of simulations and corresponding flight tests verified that the proposed adaptive control structure is robust against both constant and time-varying modeling *** the other research objective,a SAC(Stability Augmentation Control)structure was devised based on the CMG(Control Moment Gyroscope)theory in order to provide extra moment which effectively withstands the transient disturbance beyond the CDG(Critical Disturbance Gain).Furthermore,we studied the corresponding controller for the SAC via the SMC(sliding mode control)theory,while the working mechanism and performance of the SAC were verified through a specially devised prototype.

关键词： Adaptive control Disturbance rejection Ducted-fan aircraft Modeling error Stability augmentation systems

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棉花SNC2基因的克隆及表达分析

分子植物育种 2017年第1期15卷 23-29页

作者：陈方圆王冬梅李波胡文冉杨洋李晓荣范玲新疆师范大学生命科学学院乌鲁木齐830054 新疆农业科学院核技术生物技术研究所新疆农作物生物技术重点实验室新疆农业科学院棉花分子机理与分子育种实验室乌鲁木齐830091

以陆地棉(Gossypium hirsutum)TM-1为材料,利用同源克隆技术从棉花中分离出SNC2基因,以actin基因为内参,采用半定量RT-PCR分析该基因在棉花组织中的表达,并采用实时定量RT-PCR分析不同棉花品种经大丽轮枝菌侵染后,SNC2基因在不同时段、... 详细信息

以陆地棉(Gossypium hirsutum)TM-1为材料,利用同源克隆技术从棉花中分离出SNC2基因,以actin基因为内参,采用半定量RT-PCR分析该基因在棉花组织中的表达,并采用实时定量RT-PCR分析不同棉花品种经大丽轮枝菌侵染后,SNC2基因在不同时段、不同组织的表达水平。研究分析发现该基因CDS序列长度为1 308 bp,编码435个氨基酸,蛋白的等电点(p I)为6.98,理论分子量为46.93 k D,命名为SNC2,Gen Bank登录号为AOO35322.1,SNC2基因编码多肽链中包含信号肽和跨膜结构域,且亚细胞定位于质膜,编码受体类蛋白。组织表达分析显示SNC2基因在根、侧根、茎、叶和花中均有表达,茎中的表达量较高,而在苞片组织中基本不表达。该基因在接菌后的感病品种军棉1号的根和耐病品种中棉49号的茎中呈现不断上升的趋势,而在两个棉花品种的其他组织中呈现出"升-降-升"的趋势,且在不同抗性的棉花品种中的表达存在差异,暗示SNC2基因可能在不同抗性品种抵御生物胁迫过程中扮演重要角色,研究结果为进一步研究SNC2基因的功能及应用该基因提供帮助。

关键词：棉花 SNC2 基因克隆大丽轮枝菌侵染实时荧光定量

Tackling the microlensing wave effects of strong lensing gravitational waves with TAAH

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Science China(Physics,Mechanics & Astronomy) 2025年第1期Mechanics & Astronomy) .卷 257-258页

作者： Zuhui fan South-Western Institute for Astronomy Research Yunnan University

The rapid development of gravitational wave（GW） observations since their first detection in 2015 [1] has opened a completely new window for exploring the Universe. Consequently, astronomical research has entered the multimessenger era, allowing us to use different tracers to view the cosmos from various perspectives. Among others, the appealing application of gravitational wave events from compact binary mergers is that they can act as standard sirens to determine absolute cosmic distances entirely independent of standard candles. This approach is one of the most promising means to solve the current H0tension problem.

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